Inovo injection of water extracted propolis on hatchability and some physiological characteristics of broiler chickens

Abstract

The research was conducted to investigate the effect of water extracted propolis on hatchability,body weights of hatched chicks as well as some hematological traits which were represented by packed cell volume,Hemoglobin concentration ,Heterophil lymphocyte ratio,in addition to evaluation of glucose, cholesterol and plasma protein .A total of 300 embryonated eggs,10th day of incubation were used in the experiment , those eggs were randomly allocated in to 5 treatments , each treatment included 2 replicates , 30 eggs of each replicate . Eggs were injected by water extracted propolis in to 3 treatments meanwhile ,other 2 treatments were left as control,treatments were arranged as follow:T1: Negative control treatment .T2 :Posative control treatment(0.1 ml of distilled water was injected in each egg). T3: Eggs in this treatment were injected with 50 mg /ml of water extracted propolis (0.1 ml/egg) .T4: Eggs in this treatment were injected with 100 mg / ml water extracted propolis (0.1 ml/egg).T5: Eggs in this treatment were injected with,150 mg / ml water extracted propolis . (0.1 ml/egg) .Results showed no significant effect of water extracted propolis on hatchability and percentages of deformed chicks , mean while, the extracted propolis led to a highly significant effect (p > 0.01) on body weights of hatched chicks which were 44.35, 44.60 and 45.75 gm / chick in the third ,forth and fifth treatment respectively. The results indicated significant effect of the extract on some hematological values such as packed cell volume , hemoglobin concentration,whereas, the Heterophil /Lymphocyte ratio was significantly improved(p>0.01)which were 0.29,0.28 and 0.26 in the third ,forth and fifth treatment respectively . No significant effect of the extracted propolis was noticed on the plasma glucose but the plasma cholesterol was significantly reduced to 166.76 , 164.14 and 158.80 mg /100 ml in the third ,forth and fifth treatment respectively, plasma protein was elevated significantly (p>0.01) due to the injection of the extract as well ,it was 3.19, 3.93 and 5.06 gm/100ml in the third ,forth and fifth treatment respectively .It was concluded that water extracted propolis had an important effect in increasing body weights of chicks post hatching as well as improving H/L ,reducing plasma cholesterol and elevation of plasma protein . Key words : Proplis. In ovo injection.