Study of the inhibitory effect of Ethanolic extract of( Quercus robur ,Cinnamomum zeylanicum and Thymus vulgaris ) on the growth of Staphylococcus aureus isolated from clinical mastitis in cow

Abstract

The present study was designed to evaluate the inhibitory effects of three local plant ethanolic extracts (Quercus robur , Cinnamomum zeylanicum and Thymus vulgaris) against the growth of Staphylococcus aureus isolated from milk of cow infected with clinical mastits in culture media by using of agar well diffusion method. For this purpose graduate concentrates for each extract (50 , 100 , 200 , 400 )mg/ml prepared and tested .The result showed that the extract of Quercus robur was more effective followed by Thymus vulgaris and Cinnamomum zeylanicum.The statistical analysis by using ANOVA with LSD at level (p<0.05) showed that there was no significant differences between the effect of the studied concentrations of Quercus robur , Cinnamomum zeylanicum while for Thymus vulgaris we find that the concentration 100 mgml had a significant difference with the other studied concentration in inhibition of the growth of the tested bacteria. We also find that between the antibiotics the largest zone of inhibition was given by LOM followed by SPV,PI,NV,RA and CX.