تأثير الصدمة الكهربائية في تحفيز انقسامات خلايا المزارع الخلوية المشتقة من كالس السيقان تحت الفلقية لنباتات زهرة الشمس والمزروعة في قطرت الاكار المتعددة

Abstract

Sunflower friable callus used in obtaining cell suspension culture in liquid MS medium containing 2,0 mg /L.NAA and 1.0 mg/ L BA, The cell started their first division after 24hours and continued their division reaching to 7,33-8.50 x 103 cell / ml after 4 days of incubation.Culturing of cell suspension using Multiple Drop Array (MDA) technique promote the divisions of cells and the formation of cell colonies which developed to form callus primordia,Electro treatment of cell suspension enhanced division of cells, and the first division, occurs during 3-5 days the. total number of cells colonies reach to 380. Callus formed cell suspension from sunflower did not overcome the regeneration problem in this plant,