In Vetro Cell Divission of Pelargonium Pith Tissue

Abstract

Culture media were used to develop a rapid production system for geranium (Plargonium X hortorum) in vitro. Pith tissue was excised from the stem and induced to divide on White medium supplemented with10-6 M 2,4-D and kinetin. Once they have been induced to divide, they maintained on Murashige and Skoog medium supplemented with 2,4-D and kinetin, at 10-6 M. Non differentiated organs are produced in the established pelargonium pith callus. Pelargonium pith callus were obtained on medium was very friable. By gentle shaking, free cells were obtained and small cell clumps, which can be used as inocula for plante cultures. The plating efficiency has been brought to about 33% on Murashige and Skoog medium supplemented with 10-6 M 2,4-D and 6-dimethylallylaminopurine. Single cells have been observed to divide in the presence of other cells and cell clumps, but never happen in complete isolation