Imunocytochemistry Detection of M1CA Gene Receptor in Human Patients with Brucellosis

Abstract

Background: The most relevant cytokine involved in the response against the genus Brucella is interferon gamma (IFN-γ) that is mainly produced by NK cells and stimulated by the binding between their receptors NKG2D receptor and its ligand M1CA gene expressed on Brucella – host infected cells.Aim: To evaluate the relationship between host susceptibility for Brucella infection and the expression of MICA gene which demonstrated by imunocytochemitsry staining technique .Methods: Rose Bengal test was applied for 340 patient serum then Brucella blood culture was carried out for Rose Bengal positive samples. Imunocytochemistry technique was used to check the expression of MICA gene receptor on human infected cells. Moreover, the level of IFN-γ was measured in patient sera by using of ELISA technique.Results: Rose Bengal Test (RBT) revealed that 155 patients were positive. Brucella- Blood culture revealed that only eight samples gives positive for blood culture. Five samples expressed MICA gene receptors. The level of IFN-γ revealed a significant increasing(p ≤ 0.05 ) in positive samples for MICA gene receptors as compared to negative samples for MICA gene receptors.Conclusion: There is a good relationship between the expression of MICA gene receptor and immune response against Brucella that represented by an increasing of IFN-γ.