Extrication and Identification of some flavonid compounds from grape seeds Shada Sodda, Bedha Vitis vinifera L. and waste of grape juice by chromatographic liquid high-performance (HPLC)

Abstract

Four Flavonid compound have been extracted from the seeds of two different species of grapes named( Shadaa Sodda and Bedha) and waste of grape juice, the leaves were hydrolyzed to break the glycosides linking of the Flavonid glycosides by Soxhelt (Petroleum ether) at temperatures from 60 to 80 m for a period of 6 hours, followed by extracting the powder to the device for 8 hours by a mixture of solvents consisting of methanol: water: acetic acid (0.5:9.5:90), and diagnosed in the extracts as Catechin and λ- procatechin in black grape seed, and Epicatechin in white grape seed,and Catechol in the waste of grape juice, using the technique chromatographic liquid high-performance, (HPLC) , used a column separate from the type of O.D.S and the volume of minutes 10 mm (Mm) (250 mm x 2 mm) , Mobile phase is bytanol: acetic acid: water (374:1:11) h / h , a Flow rote1.5 ml / min, Temperature 30 m, and the wave length) the user is 280 nano-meter of the detector.