دراسة وظيفية ووراثية عن بعض تأثير ازالة المبايض على الدماغ والحبل الشوكي في اناث الارانب المحلية

Abstract

The present study was conducted to determine the impact ovarectomy in rabbits on the nervous tissue of the brain and spinal cord and some changes to the standards physiological and molecular genetic of the gene Gadd 153.16 animals were divided at randomuy from the adult rabbits into two groups rabbits( 8 / group ) were considered the control group G1 ) ( while the second group was treated to remove the ovaries and considered G2 for six months )Samples collected by direct cardiac puncture (heart puncture)and drag Alambashrell-CSF on order to the following criteria:-High density lipoprotine-cholesterol HDL-c, Low density lipoprotine-cholesterol LDL-c, Very low density lipoprotine-cholesterol VLDL-c, Total cholesterol TC, TriacylglycerolTG, Estrogen hormone E2, glucose and total protein of CSF and serum Blood in the brain and spinal cord in addition to study changes in the molecular genetic of GADD153 gene in ovarictomized female rabbits ovaries removed using local technology. Real time PCR The results of the current study : -- Showed significant decrease E2andprotein andHDL-c (P>0.05) in G2. also there ware significant increase (P>0.05)glucose andLDL-c and ,TC,TG, VLDL-c in G2 compared with the control group G1.The result also - Showed significant decrease(P>0.05) the level of glucose and a significant increase (P>0.05)glucose in the cerebrospinal fluid of G2 group compared with the control group G1.- the molecular Genetic study results indicated higher gene expression to gene Gadd 153 compared with the control group G1.