إلاكثار الدقيق لنباتات الأناناس Ananas sativas باستخدام تقنية زراعة الأنسجة

Abstract

Lateral buds of Ananas sativas were extracted and cultured on MS medium after sterilized. All cultures were incubated at 25±2°c with 16hr/day (1000 Lux) for 60 days. Data of leaves, roots, shoots per plant, their length, fresh, dry weights and percent of rooting were investigated. The results revealed that addition of BA at concentrations ( 0.24 , 0.5, 1 )mg/L caused significant increasing in branches per plant and fresh weight. Concentration at 1g/L showed higher mean of branches per plant (5.6) as compared with control (2.4), While 0.5 mg/L showed higher mean in leaves per plant (9.4) as compared with control which gave higher mean in length (3.78cm).Rooting results showed that adding NAA to medium effected significantly on percentage and roots per plant, fresh and dry weights. Concentrations at 0.5, 1, 1.5 NAA showed higher rooting percent (100%) after 4 weeks.Results of Acclimazation showed that petmose gave higher success percentage (100%) after two months, this percent was decreased according to the petmose percent.