THE ANTIMICROBIAL ACTIVITY OF BACTERIOCIN FROM PSEUDOMONAS FLUORESCENS AGAINST PATHOGENIC BACTERIA

Abstract

In this study four isolates of Pseudomonas fluorescens were tested for bacteriocin production by the agar block method. Clinical bacterial isolates like; Aeromonas hydrophilia, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Streptococcus faecalis and Vibrio cholerae(enaba) were used as indicator isolates. All the isolates were positive as produce with a wide range effect on gram positive and negative bacterial growth, with diameter (8-21) mm.The results showed that Pseudomonas fluorescens P1 inhibit the bacterial growth of the tested isolates with a range of inhibition zone (8-18) mm. except Vibrio cholerae(enaba) while P2 inhibit the bacterial growth of the tested isolates and the range of inhibition zone was (7-21) mm . except Vibrio cholerae(enaba) and Streptococcus faecalis .On the other hand P3 inhibit the growth of the isolates with a zone of inhibition between (10-16) mm. except Streptococcus faecalis and Klebsiella pneumoniae .The isolate P4 inhibited the growth of all the tested isolates with a range of inhibition zone between (8-19) mm. Escherichia coli was the most affected bacteria by bacteriocin of P. fluorescens, followed by Salmonella typhi , Staphylococcus aureus , Streptococcus faecalis, Klebsiella pneumoniae , Vibrio cholerae(enaba) and Pseudomonas aeruginosa