Table of content

Muthanna Medical Journal

مجلة المثنى الطبية

ISSN: 2226146x
Publisher: Al-Muthanna University
Faculty:
Language: English

This journal is Open Access

About

Muthanna Medical Journal (MMJ) is the official journal of Muthanna Medical College, a semiannual peer-reviewed online and print journal. The MMJ allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional/subject-based repository.
The Journal follows the ICMJE's Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals.

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Contact info

Postal Mail
Muthanna Medical School
Samawah
Tel: 0782542-5669
Email: yousif_ghaly@yahoo.com

Table of content: 2018 volume:5 issue:1

Article
Molecular detection of multidrug-resistant mycobacterium tuberculosis in Al Muthanna province

Authors: Haider H. Mitab
Pages: 1-11
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Abstract

Multidrug-resistant tuberculosis (MDR-TB) is a global problem that many countries are challenged with. Rapid and accurate detection of MDR-TB is critical for appropriate treatment and controlling of TB. The aims is detection of multidrug-resistant Tuberculosis from cultured samples by using Polymerase Chain Reaction. A total of 30 M. tuberculosis isolates from cases with diagnosed TB by GeneXpert, AFB and Culture on L. J media after incubation period from 3-8 weeks, DNA extraction from bacteria colonies. Resistant isolates were tested for characterization of mutations in the rpoB, KatG InhA1 and IhA2 genes by Real Time PCR. The results of the real time PCR showed that mutations of genes (rpoB, katG, inhA1 and inhA2) that were responsible for resistance to rifampicin and isoniazid. The test showed positive results for resistance genes (20%, 10%, 6.6%, 10% Respectively) as well as note that the values ​​of Ct for this test ranged from (12-38.25), and the melting points of the genes were between (85-88.5 Co). Real time PCR results identified three mutations of MDR (rifampicin and isoniazide) resistance genes, whereas there was one MDR mutation of molecular diagnostic results with the GeneXpertMTB/RIF test for rifampicin. When comparing the results of the Real time PCR and GeneXpert tests at the level of the genetic mutation with rifampicin, the real time PCR test showed four resistance mutations for the rpoB gene for both new cases and relapse tuberculosis as well as one rpoB mutant for under treatment patient. Both molecular tests have agreed to identify one rpoB mutant in the case of failure TB treatment.


Article
C-Reactive protein in community acquired pneumonia

Authors: Mohammed Abed Abdulhussein
Pages: 12-20
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Abstract

C-reactive protein (CRP) is an acute phase protein synthesized by hepatocytes. In response to infection or tissue inflammation, CRP production is rapidly stimulated by cytokines, particularly interleukin (IL)-6, IL-1 and tumor necrosis factor. The aim of the present study is to evaluate the prognostic value of CRP levels at admission as an indicator of the severity or complication of CAP. Fifty patients with primary diagnosis of community-acquired pneumonia during March 2013 to February 2014. A 25 patients were males and 25 females. The CRP measured by CRP-latex Slide agglutination method. Also patient underwent chest-x ray and patients were categorized into patients with CRP ≥ 100 mg/L and CRP < 100 mg/L22, and the complications (pleural effusion and lung abscess) were correlated to possible risk factors (HT, DM) and smoking. There is a significant increase in complication of CAP in patients with high CRP level (CRP ≥ 100 mg/L) ,so that CRP is highly sensitive, specific and accurate test to predict complication in CAP. The CRP affected by gender (female), age and diabetes mellitus in patient with CAP. There is no significant correlations between CRP level and smoking and hypertension in patient with CAP.


Article
Antibacterial efficacy of surgical scrubbing solutions

Authors: Bassam F. Izalddin
Pages: 21-34
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Abstract

Surgical scrubbing with antibacterial solutions is an important step in surgical operations to reduce the incidence of surgical site infection. The aim of study is to evaluated superiority of one of three surgical scrubbing solutions commonly used (povidone iodine, chlorhexidine and aqueous alcohol) by studying their antibacterial action on the skin of the hands of the operating personale. Ninety hands of surgeons, assistants and nurses were included in this study at Al Kindy Teaching Hospital from 1 June, 2011 to 1 Feb, 2012. Divided into three groups each with 30 hands according to the antiseptic solution used for scrubbing (chlorhexidine ( group 1); povidone iodine (group 2) and ethanol alcohol (group 3), hand prints were cultured as a baseline, immediately and 30 minutes after scrubbing for both gram +ve and gram –ve bacteria and a total of 540 culture medias were studied. In our study Staphylococcus aureus was the only gram positive transient bacteria found in the culture medias with a baseline ranged (0-5) colonies in group 1, (0-5) colonies in group 2 and (0-7) colonies in group 3 and all were removed immediately after scrubbing with all antiseptic solutions. Staphylococcus epidermidis was the only residents bacteria found in all the hands with a baseline ranged (10-60) colonies in group 1, (12-62) colonies in group 2 and (10-56) colonies in group 3. This bacterium was reduced to low levels immediately after scrubbing with all antiseptic solutions and completely removed after 30 min in group 2, 3 and reduced to lowest level in group 1. Gram –ve bacteria was not found in the entire three groups. In addition to that, fungi were found in all our study groups in a range of (0-7) colonies in groups 1, (0-8) colonies in group 2 and (0-8) colonies in group 3 and all of them were removed by scrubbing with all antiseptic solutions. We concluded that Povidone iodine was more effective in eradicating or reducing of both gram +ve resident and transient bacteria and fungi.


Article
Prevalence of urinary tract infections in diabetic patients

Authors: Mohammed Challoob Murad
Pages: 35-39
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Abstract

There is evidence that patients with diabetes have an increased risk of urinary tract infections (UTIs). UTI is the most common bacterial infection in diabetic patients. The study included 500 diabetic patients attended at a private urology clinic in Wasit south east of Iraq between January and December 2017. From the total number of patients, 60 had positive urine cultures(12%). The most frequent bacteria involved in UTI was Escherichia coli (70%). We concluded that UTIs are frequent in diabetic patients and urine culture should be performed to give proper antibiotics and to prevent recurrence.


Article
Use of halothane vaporizer for the administration of sevoflurane during general anesthesia

Authors: Basim Hrez Ali
Pages: 49-55
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Abstract

The vaporizer based on specific saturated vapor pressure for each inhalation anesthetic agent. The purpose this study is to determine the precise corresponding sevoflurane concentrations when halothane vaporizer is used for supplying it. This study is carried out by using a mathematical equation based on the difference in saturated vapor pressure, splitting ratio of halothane and sevoflurane at different delivered concentration dial of halothane vaporizer. The data of this study provides a comparative table that help to use halothane vaporizer to provide a precise known delivered sevoflurane concentration. The results concluded that halothane vaporizer is suitable to deliver sevoflurane concentrations (up to 3%) that can be used for maintaining general anesthesia.


Article
Effect of NRAMP1 Gene polymorphism on levels of (TNF-α and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq

Authors: Ghada B. Alomashi
Pages: 56-65
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Abstract

Cutaneous leishmaniasis (CL) is vector-borne disease, and endemic in most regions of Iraq especially with poor populations. Natural resistance-associated macrophage protein 1 (NRAMP1) gene play an essential role in susceptibility to CL and disease pathology, NRAMP1 influences a production and activation of pro-inflammatory cytokines (TNF-α and IL-1β). Pro- and anti-inflammatory cytokines play an essential role in susceptibility/ resistance and the immunopathogenesis of Leishmania infection, these cytokines are crucial factors in the initiation and enhances of protective immunity against Leishmania infection, this study aimed to studying effect of polymorphism in NRAMP1 genes on cytokines secretion, and their effect in susceptibility to CL infection. Samples of blood were collected from (60) patients with CL and (32) apparently healthy controls. Polymorphism of NRAMP1 (D543N) detected in patients and control groups by PCR-RFLP technique. While (TNF-α and IL-1β) cytokine concentration detected by ELIS technique using a quantitative sandwich enzyme immunoassay technique, Results indicate to effect of NRAMP1 Gene Polymorphism on levels of (TNF-α and IL-1β) cytokines and this a clearly recorded in present study were A allele is associated with lower levels of (TNF-α and IL-1β) in patients and control groups compression to that absorbed in allele G with statically significant (P ≤ 0.05).

Table of content: volume:5 issue:1