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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2007 المجلد: 6 العدد: 1

Article
البوليمـرات المتعادلة الشـحنة غير المكثفـة لـ DNA

المؤلفون: عمر محمد عباس السامرائي
الصفحات: 1-18
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الخلاصة

الكلمات الدلالية


Article
PRODUCTION OF DIAGNOSTIC KIT FOR SYPHILIS
إنتاج عدة تشخيصية لمرض السفلس

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الخلاصة

ABSTRACT This study involves the use of bull myocardium and egg yolk as a source for obtaining cardiolipin and lecithin respectively, which are considered as elementary materials in the preparation of the diagnostic kit of the syphilis disease (Rapid plasma reagin circle card test). The findings have also shown the effectiveness of the antigen prepared in this study with both sera of the syphilitis patients and the standard sera as well as comparing the effectiveness of that antigen prepared in this study with the antigen imported from Biokit Spanish company. The antigen prepared in this study has been evaluated by the directorate of teaching laboratories at the hospital of Medical city. The results of this evaluation is that the antigen prepared in this study is good and obtained good results with both syphilitic serums and standard sera. In addition the anti-cardiolipin antibodies prepared in this study have proved effectiveness towards the antigen prepared in this study and towards the antigen imported from Biokit. _______________________________________________________________ Keywords: Syphilitis patients, Biokit, anti-cardiolipin antibodies.

الكلمات الدلالية


Article
دراسة دور حامض السالسيليك في تحييد بلازميدات المقاومة لنوعين من بكتريا الزحار

المؤلفون: سهاد ناجي كلف
الصفحات: 31-41
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الخلاصة

ABSTRACT Two strains of Shigella (Sh.sonnei, Sh.dysenteriae) were isolated from patients suffering from diarrhea. The strains were screened for their resistance to some antibiotics, and the screening results indicated that they were resistant to Ampicillin, Cefotaxime, Kanamycin, Streptomycin and Nalidixic acid. Resistance to: Tetracyclin and Chloramphenicol varied between the strains. One of the strains (Sh.sonnei) was found to have the ability to produce bacteriocin. The two strains (Sh.sonnei and Sh.dysenteriae) contained large plasmid band and various small bands. Results of conjugation experiments between the Shigella as a donor and the standard E.coli strain (MM294) as a recipient indicated the transfer of resistance of Tetracyclin , Streptomycin , Nalidixic acid and Ampicillin in addition to bacteriocin production , this may refer to their location on a conjugative plasmid. Resistance to Cefotaxime was not transferable by conjugation, but by transformation. Transformation also expressed resistance to Ampicillin , Nalidixic acid and Tetracyclin which may indicate the presence of another copy of resistance genes on a non-conjugative plasmid in Shigella. The study utilized the salicylic acid (150mg/ml) effects on the growth and as a curing agent , the results showed there was no effect of this acid on the growth of Shigella spp , while the results found a curing effect on the resistance genes of both species of Shigella. Keywords: Salicylic acid, curing, shigella. PDF created

الكلمات الدلالية

Salicylic acid --- curing --- shigella


Article
PURIFICATION OF GLUCOSE AND GALACTOSE SPECIFIC LECTINS FROM NIGELA SATIVA SEED
تنيقة اللكتينات المتخصصة للارتباط بالكلوكوز والكالاكتوز من بذور الحبة السوداء(N.sativa)

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الخلاصة

ABSTRACT Glucose specific lectin (GL) and galactose specific lectin (GaL) were purified from Nigela sativa at the first time in country. Purification steps included fractionation with ethanol (50-75%), Dialysis and affinity chromatography in sephadex (G-50) column for (GL) specific lectin and sepharose (4B) Column for (GaL) specific lectin. Lectin were purified to homogeneity as indicated by the presence of one band in polyacrylamid gel electrophoresis in the absence of denaturing conditions. The molecular weight of purified lectins measurements by gel filtration suggest values of 25000 and 50000 dalton for (GL) and (GaL) specific lectins respectively, and 25.000, 50.000 dalton by Polyacrylamide gel (SDS) electrophoresis. Keywords: Glucose specific lectin, galactose specific lectin affinity chromatography, polyacrylamid gel. PDF created


Article
EVALUATION OF ANTIMICROBIAL ACTIVITY OF WATERY AND ALCOHOLIC EXTRACTS FOR MATRICARIA CHAMOMILLA ON INHIBITION OF GROWTH OF GRAM POSITIVE PATHOGENIC BACTERIA ISOLATED FROM PHARYNGITIS AND TONSILLITS CASES
تقييم فعالية مستخلص ازهار البابونج المائي و الكحولي على تثبيط نمو البكتريا المرضية الموجبة لصبغة كرام المعزولة من حالات التهاب البلعوم واللوزتين

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الخلاصة

ABSTRACT The effects of Matricaria chamomilla plant extract were studied in respect to their of gram positive bacterial growth inhibition. were isolated from cases of pharyngitis and tonsillitis. alcoholic and hot water extracts of the plants as well as their dried powders were prepared. The preliminary chemical tests revealed acidic pH of all extracts. The dried powder, watery and alcoholic extracts of M. chamomilla contained resins,tannins,couumarines ,phenols, flavonoids and avery little amount of glycosides in its watery extract and dry powder, with a small amount of alkaloids in its alcoholic extract.The alcoholic extract of M. chamomilla showed more patent inhibitory effect on resistant bacteria than its watery extract and the best effect was on growth of Strept.pneumoniae and Strept.pyogenes inhibition zone diameter 27.0 mm. In the present study, the Minimum inhibitory concentration (MIC) and Minimum bacteriocidal concentration (MBC) of the plants extracts were measured for the more predominant gram positive isolates and the results varied according to different kinds of plant extracts and different types of bacteria. The least values of MIC and MBC were for alcoholic extract of M. chamomilla on Streptococcus pyogenes and Streptococcus pneumoniae which valued 20% , 30% respectively. It has been noticed that the watery extracts of M.chamomille, have a sedative effect when given dosing 5 g/kg for laboratory mice , these extracts however, showed a synergistic sedative effect when mixed with pentobarbitone and caused prolongation of sleeping time in experimental mice .Neither the alcoholic nor the watery extracts of the plants showed any toxic effect on the laboratory mice after oral dosing of 2.5 –15 g /Kg B.W. Key word: Watery and alcoholic extracts, Matricaria chamomilla , Pharyngitis ,Tonsillitis.


Article
INHIBITION ACTIVITY OF BARK CASSIA CINNAMON EXTRACT AGAINST SOME MICROORGANISMS
التأثير التثبيطي لمستخلصات قلف نبات القرفة (الدارسين) الصيني في بعض الأحياءالدقيقة

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الخلاصة

ABSTRACT The study was conducted to evaluate the inhibitory activity of the Cassia cinnamon bark extracts (cold watery, hot watery, ethanolic and oily extracts) on some bacterial tested strains, which include three gram negative strains Escherichia coli 25922, Salmonella typhimurium and Ps. aeruginosa 15442, and one gram positive strain Staphylococcus aureus 25923, in addition to the yeast Candida albicans 10231, using the well diffusion method yeast. It was noticed that the inhibitory activity of the four extracts in the microorganisms tested strains was different according to the difference of the extract solvent and the microorganism. The effect of oily extract in a concentration of 25% was highly significant effect in accordans to the rest extracts in the inhibition of the microorganism tested strains as the diameter of the growth inhibition areas 25.67 , 24, 25, 24 and 33 millimeter in the bacteria E. coli, Staph. aureus, Sal. typhimurium, Ps. aeruginosa and yeast C. albicans respectively. Coming next is the ethanolic extract in a concentration of 25% while the cold water extract in 35% concentrate showed the lowest inhibitory effect. Sal. typhimurium showed higher resistance to the cold water 35%, hot water 35% and ethanolic 25% extracts activity in comparison to the other tested strains. Key words: Cinnamon, Extracts, microorganisms, Inhibition activity. PDF created

الكلمات الدلالية

Key words: Cinnamon --- Extracts --- microorganisms --- Inhibition activity. PDF created


Article
THE EFFECT OF CHANGES IN CULTURE MEDIA, pH AND TEMPERATURE ON IN VITRO FERTILIZATION AND PREIMPLANTATION EMBRYONIC DEVELOPMENT OF MICE
أثير التغييرات في الأوساط الزرعية والباهاء ودرجة الحرارة على الاخصاب الخارجي وتطور الأجنة قبل الانغراس في الفئران

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الخلاصة

ABSTRACT The present study was conducted to investigate the effect of different types of culture media, degrees of temperature of culture medium and wide range of pH within culture medium on the percentages of in vitro fertilization of mouse oocytes and embryonic development. Nine hundred and twenty four oocytes were recovered from eighty one superovulated mice. Groups of mature oocytes were incubated with active spermatozoa within different culture media (Ham’s F-10, Medi-Cult. IVF, TCM-199, Earl’s + TCM-199 and RPMI-1640) at different degrees of temperature (36, 37, 38 and 39), as well as, the pH of culture medium was changed (range: 6.9-8.1) in 5% CO2 for 18-20 hour. Percentages of in vitro fertilization and abnormal embryonic development were assessed. The best results for IVF and normal embryonic development were achieved with RPMI-1640 medium at temperature 37 oC and acidity (7.3-7.4) of medium. However, best results for in vitro development of mouse embryos were obtained by using TCM-199 medium and Ham’s F-10 medium at temperature 36 oC and acidity (7.3-7.4). In conclusion, the mouse spermatozoa and oocytes are highly sensitive to changes in the environmental conditions. In addition, conditions of culture medium and its selection are may be the main limited factor for improvement in vitro fertilization and embryonic development in mice. Further biochemical and molecular studies are recommended to assess the effect of culture medium on content of RNA and protein synthesis of different stages of mouse embryo. Key words: Superovulation, in vitro fertilization and embryonic development. PDF created


Article
PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE IV FROM PSEUDOMONAS AERUGINOSA
التنقية الجزئية وتوصيف البروتييز Pseudomonas aeruginosa منIV

المؤلفون: رباب عمران
الصفحات: 94-106
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الخلاصة

Clinical strain Pseudomonas aeruginosa No.3 was isolated from human corneal ulceration. The bacterial cells secreted the extracellular protease in liquid culture. The enzyme was partial purified 191 fold from culture filtrate by sequential steps such as salting out with ammonium sulfate precipitation (80% saturation), ion exchange CM- Cellulose Chromatography, and by Sephadex G-75 Gel filtration. Characterization study of the partially purified enzyme revealed that the enzyme had an optimum activity at pH 9.5 and the activity was stable in the alkaline pH range (8- 10 )for 30 min. Enzyme activity toward casein increased with temperature raise up to 35°C and maximal activity was attained at 45° C. The enzyme was stable at temperature under 30˚C and approximately 90% of the activity was abolished by incubation of the enzyme at 60 ° C for 40 min or at 80 ° C for one min. Protease IV activity was partially inhibited by phenylmethylsulfonyl fluoride (20%) and Diisopropyl fluorophosphate (75%). EDTA at 50mM caused a 22% inhibition of protease activity, which suggested that the enzyme is a serine protease. The reducing agents dithiothreitol (1.0 mM) and 2- mercaptoethanol (150-mM) also demonstrated complete inhibition of the enzyme, which suggests that the enzyme protein containing disulfide bonds could be important in maintaining the molecular conformation required for activity. __________________________________________________________


Article
KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE IN THE MUCOSA OF MOUSE AND RAT SMALL INTESTINE
الخواص الحركية لإنزيم فوسفو فركتوكايناز في غشاء الأمعاء الدقيقة المخاطي للفأروالجرذي

المؤلفون: صاحب علي الأطرقجي
الصفحات: 107-118
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الخلاصة

ABSTRACT The regulatory properties of mouse jejunal mucosa phosphofructokinase have been studied in crude extracts freed from low molecular weight effectors on Sephadex G-100, and compared to those of the rat. Both isoenzymes displayed cooperativity with respect to fructose 6-phosphate at pH 7.0 in the presence of inhibitory concentrations of ATP. The rat isoenzyme was activated to a greater extent by AMP and P, and inhibited to a greater extent by creatine phosphate than the mouse isoenzyme. Both isoenzymes were strongly activated by glucose 1,6-diphosphate and strongly inhibited by citrate. The general similarity in properties of the two isoenzymes reflects the fact that both rat and mouse intestinal mucosa are characterised by high rates of aerobic glycolysis. keywords: Phosphofructokinase isoenzyme, fructose 6-phosphate. PDF created


Article
PARTIAL PURIFICATION AND SOME PROPERTIES OF PROTEASE FROM MICROSPORUMCANIS
التنقية الجزئية وبعض صفات البروتيز من Microsporum canis

المؤلفون: حيدر شخير --- رباب عمران
الصفحات: 119-131
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الخلاصة

ABSTRACT Clinical strain Microsporum canis was isolated from specimen of patient with Tinea corporis in arm and it has ability to produce extra cellular keratinolytic protease in a broth containing human hair. The enzyme was partial purified 35.8- fold from culture filtrate by sequential steps through salting out with ammonium sulfate precipitation (80% saturation), ion exchange Chromatography by batch wise ion exchange by CM- Cellulose cation resin and anion resin by DEAEcellulose column. The partially purified enzyme had an optimum activity at pH 9 and maximum activity at pH11. The activity was stable in the alkaline pH 9 for 30 min at 25°C. Enzyme activity toward casein increased when temperature raised more than 20°C and maximal activity attained at 55° C. The enzyme was stable at temperature under 25˚C and approximately 80% of its activity abolished by incubation of the enzyme at 60 ° C for 30 min. Protease had activation energy equal 3.314 Kcal/Mole that to be able to transform casein to product. On the other hand, the activation energy for denaturation was equal to 49.675 Kcal/Mole. The result of this experiment demonstrated that the enzyme is heat labile. _________________________________________________

الكلمات الدلالية

Keywords: Microsporum canis --- Protease --- purification --- properties

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