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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2007 المجلد: 6 العدد: 2

Article
Study about Stem Cells and Some Application Therapy
الخلايا الجذعية وبعض تطبيقاتها العلاجية

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الخلاصة

Stem cells characterize as a primitive cells and have the ability to divide and make new stem cells similar to it or produce different specialized cells , so these cells remained unspecialized until receive special signals push it to evolutes into special cell. Most studies on the animal cell indicated that the presence of this stem cells which able to differentiation to any kind of body cells. Experiments revealed the ability of these cells to transform to any tissue or special organ in the body like, heart, muscle and nerve cells, and these discoveries bring back the hopes to use stem cells medically to compensated the damage part and curing some dangerous diseases like , autoimmune diseases , diabetes , Parkinson's , and other diseases , in addition knowing other different kind neither it is embryonic , germen , adult, or cancer cell, and cellular organization ways and modern techniques about discover it through use surface marker or genetic engineering techniques and prevent moral fears that connected to take these cells from the embryo. This still continuous to find many molecular properties which characterizes stem cells and investigate new mechanism for different cellular treatment of dangerous disease which still under research and discovery. ___________________________________________________________

الكلمات الدلالية

Keywords: stem cell --- embryonic cell --- diabetes.


Article
THE EFFECIENCY OF HENNA LEAVES EXTRACTS AND SOME FUNGICIDE TO REDUCE THE FUNGAL CONTAMINATION OF DATE PALM (Phoenix dactylifera L.) TISSUE CULTURES
كفاءة مستخلص أوراق نبات الحناء وبعض المبيدات الفطرية في تقليل التلوث الفطري في مزارع أنسجة نخيل التمر Phoenix dactylifera L.

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الخلاصة

This study was included to identify the fungal contamination associated with callus tissue culture of date palm (Phoenix dactylifera L.) and the attempt to find the fittest methods to decrease it. The isolation and identification results of fungi explained the isolation of ten fungal genera. The fungus Asperillus niger recorded the highest percentage of frequency which was 16.8%, followed by Penicillium sp.1, while the frequency percentages 10,8,7 and 5% were recorded in Alternaria alternata ; Epicoccum sp.; Trichoderma harzianum and Nigrospora sp., respectively. The results proved the high antifungal activity of ethanolic Henna (Lawsonia inermis) leaves extracts on the radial growth inhibitory percentage which was 85.15%, with significant difference than Methanolic extract (53.16%). The extract concentration 1% recorded the highest percentage of inhibition (81.10%), also the results showed the failure grow of Epicoccum sp. and Nigrospora sp. on the liquid media (PD Broth) which treated with ethanolic extract concentrations 0.5 and 1%. The lowest inhibition percentage (65.15%) of dry growth was recorded in T. harzianum at concentration 0.5%. The results of fungicides (Benlate; Beltanol and Cryptanol) survey elucidated the high inhibitory efficiency of Benlate (1gm/L), which led to inhibite the growth of contaminated fungi on solid and liquid media completely. The results revealed that the fungicides Benlate (1gm/L) had no effect on the studied parameters of embryogenic callus of date palm (Berhi and Ashkar cv.). The results of fresh, dry weight of embryogenic callus and the number of somatic embryoes had no significant difference than control. Also the treatment of Benlate led to decrease the percentage of fungal contamination which was 100% and reached 16.6% in Benlate, while the treatment of ethanolic Hennaleaves extract (0.5 and 1%) had a negative effect on the growth of callus (Berhi and Ashkar cv.). The fresh and dry weight of embryiogenic callus reduced significantly in the concentrations 0.5 and 1% in contrast with control treatment results. The number of somatic embryoes was 4.16 in control, reduced significantly and reached 0.66 embryoes in conc. 0.5 and 1% of ethanolic extract. Key words: Date Palm, Henna, tissue culture, Phoenix dactylifera , fungal contamination

الكلمات الدلالية

Date Palm --- Henna --- tissue culture --- Phoenix dactylifera --- fungal contamination


Article
Phoenix dactylifera L.توصيف انزيم اليورييز المنقى من نوى تمر الزهدي

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الخلاصة

Characterization of urease extracted from Zahdi seeds showed that the molecular weight was 409,260 and 89,125 Daltons as determined by gel filtration and SDS- polyacrylamide gel electrophoresis, respectively. This indicates that the enzyme is composed of five similar units bounded by disulfide bonds, the isoelectric point (pI) was 5.1. The optimum pH for the enzyme activity and stability were 8 and 7.5-8.0 respectively. The optimum temperature of the enzyme activity was 40 oC and that for stability was 35 oC for 15 minutes, using sodium phosphate buffer at pH 8.0 and the maximum activity of the enzyme was at ionic strength of 0.25M. Kinetic studies showed that Km and Vmax values were 10.53 and 1110 mmole/ min, respectively, when urea was used as substrate


Article
التحري الوبائي عن جرثومة السالمونيلا

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الخلاصة

ABSTRACT This study enrolled 350 infants and young children less than 10 years aged with bloody diarrhea, seeking medical advice at AL- Alwia children hospital – Baghdad during a period extending from the 1st of June till the end of October 2001. The results showed isolation of 34 isolate belong to Shigella , Salmonella, EHEC and Campylobacter depending on routine biochemical test in addition to use Api system. An epidemio logical case index was applied in the investigation of small out break caused by 13 isolate of S. typhimurium which emerged during the study period. Markers used were bio-typing, sero- typing and plasmid profile. Also the results of plasmid profile indicated differences in the plasmid bonds arrangement isolates and no differences in 2 isolates.

الكلمات الدلالية


Article
EXTRACTION, PURIFICATION AND CHARACTERIZATION OF CAMELS PEPSIN (Camelus dromedarius
64 استخلاص وتنقية وتوصيف الببسين المعزول من المعدة الرابعة للجمال Camelus dromedarius

الصفحات: 64-76
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الخلاصة

ABSTRACT The results of the current study revealed that chymosen and pepsin are the major enzymes constituents of the camel aqueous abomasal homogenates. Enzymes were precipitated with ammonium sulphate at 35-80% saturation, and further purification involved chromatography on DEAE cellulose-32 and gel filtration on Sephadex G-100. Three activity peaks (P1, P2 and P3) were eluted at 0.24, 0.43 and 0.54 M NaCl, and P2 and P3 completely inactivated ribonuclease confirming that they are pepsin (A and B), whereas P1 showed very little activity indicating that it is chymosin. In addition to that, the milk clotting activity was distributed in order of 11.62, 52.98 and 35.40% for chymosin, pepsin A and B, respectively. Pepsin A and B had molecular weight approximate 35000 and 36500 Dalton respectively. Also, pepsins are not glycoproteins, but contained 0.7 and 1.1 mol of organic phosphate per 1 mol of protein, for pepsin, A and B respectively. The results also showed that both isoenzymes neither serin or thiol nor metallo proteases, and tyrosine may not participating at the active site of the pepsins. Key words: Camel pepsin, Purification, Camelus Dromedarius.

الكلمات الدلالية

Camel pepsin --- Purification --- Camelus Dromedarius.


Article
THE EFFECT OF OIL EXTRACTED FROM THYME Thymus vulgaris IN CANDIDASPECIES AND BACTERIA IOSLATED FROM CHILDREN INFECTED WITH ORAL THRUSH
تأثير المستخلص الزيتي لأوراق نبات الزعتر Thymus vulgarisفي نمو الخمائر والبكتريا المعزولة من أفواه الأطفال المصابين بداء السلاق الفمي

الصفحات: 77-87
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الخلاصة

ABSTRACT A total of 120 sawps were collected from children (age between new born and 10 years) who infected with oral thrush. Five candida species which belong to C. albicans; C. tropicalis; C. guillermonbdii; C. glabrata; C. kefyr and six bacterial isolates which is Staphylococcus aureus ; S. epidermidis; Streptococcus pyogenes; S. pneumoniae; Escherichia coli; Klebsiella spp. were diagnosed. The oil extracted of thyme was obtained by hydrodistillation method using Glvengar apparatus; agar well diffusion method was used to study the effect oil extracted against yeasts and bacterial isolate. The highest percentage of inhibition was 63.4%, 61.7%, 60.5%, 59.7%and 59.7%within 30%concentration for yeasts C. Albican; C. Tropicalis; C. Glabrata; C. kefyr and C. guillermondii respectively. Also the highest percent of inhibition was 39%, 36.1% and 27.5% with 20% concentration for Staphylococcus aureus, Streptococcus pyogenes and E. coli respectively. KeyWords: Thymus vulgaris , candida sp. , Oral thrush

الكلمات الدلالية


Article
THE IMMUNE RESPONSE TO DIPHTHERIA TOXOID IN CHILDREN VACCINATED WITH DPT VACCINE
الاستجابة المناعية لذوفان الخناق في الأطفال الملقحين باللقاح الثلاثي

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الخلاصة

In this study, 64 blood samples were taken from the healthy children after 2-4 weeks of vaccination by DPT. The children were divided into three groups according to the number of the doses of vaccine .The aged of children of the first three groups ranging from 6-55 months while the age of the fourth group ranging from 6-12 years, received the fifth dose of vaccine before 2-8 years. The control group included fifteen healthy children. They were aged between 11-85 months and they had not been received DPT vaccine before. Neutralization test was used to assess the concentration of the diphtheria antitoxin (DAT). The results showed that the serum first three groups of children contain a concentration of DAT higher than 0.1 IU/ml at the percentage 98.4% while the results of the fourth and control groups showed reduce of the DAT concentration less than 0.1 IU/ml at the percentage 60.86% and 100% respectively. Also the result showed an increase in the concentration of DAT as the number of the vaccine doses increased. Where the fifth dose showed significant differences in DAT concentration (p ≤ 0.001, 0.01, 0.05) in comparison with control, the third and the fourth doses respectively. The results showed negative direct correlation between the age of fourth group and the concentration of DAT (r= -0.730) and also there was positive direct correlation between the number of days after the vaccination and the concentration of DAT (r = 0.532) for the first three groups of the vaccinated children.

الكلمات الدلالية

Key words: DPT --- Vaccination --- DAT --- Neutralization test


Article
EVALUATION OF INHIBITION ACTIVITY OF Ocimum basilicum LEAVES EXTRACTS ON SOME PATHOGENIC MICROORGANISMS
تقييم فعالية مستخلصات أوراق نبات الريحان Ocimum basilicum في تثبيط بعض الأحياء المجهرية المرضية

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الخلاصة

This study included the qualitative chemical tests of some basic ingredients in Ocimum basilicum leaves. The results showed that the leaves contain glycosides, alkaloids, tannins, resins, saponins, comarins, flavonoides and phenols. The inhibition activity of O. basilicum leaves extracts were studied on some pathogenic microorganisms like Staphylococcus aureus, Salmonella typhi, Escherichia coli, Vibrio cholerae, Aeromonas hydrophila, Serratia marcescens, Klebsiella pneumonia and Candida albicans. The results showed that the inhibition activity of the extracts of O. basilicum leaves varied with different extraction solvents and microorganisms. The organic phase of petroleum ether showed the best effect of inhibition activity on the growth of S. marcescens and A. hydrophila which was 21 and 25 mm respectively. While the aqueous phase of chloroform extract had the best effect on S. aureus and A. hydrophila which reached to 19 and 22 mm respectively.

الكلمات الدلالية

Key words: Ocimum basilicum --- pathogenic microorganisms


Article
PREPARATION AND EVALUATION OF LOCAL MEDIUM FOR CULTURE AND GROWTH OF Trichomonas vaginalis ISOLATED FROM PATIENTS IN MOSUL, IRAQ
تحضير وتقييم وسط محلي لزرع ونمو طفيلي المشعرات المهبلية المعزول من المرضى في الموصل / العراقTrichomonas vaginalis

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الخلاصة

ABSTRACT This study was conducted to prepare a local culture medium from orange leaves for cultivation of Trichomonas vaginalis. Five culture media, namely: Oxide Trichomonas media (CM 161), CPLM, Barbarowski, Protose pepton and Pavlova, which are used for clinical diagnosis of vaginal trichomoniasis were compared in vitro to this new medium. Growth studies using fresh clinical isolates from different patients showed clear ability of Trichomonas to grow on this medium. The organisms inoculated initially (25´l04 cm-3) into this media reached a population of (78´l04 cm3) organism in 72 hours. Although (CM 161) and (CPLM) media allows more prolific growth over a shorter period of time (24 hours). It was obvious that the orange leaves media (OLM) was suitable for detecting T. vaginalis in patients with vaginitis.

الكلمات الدلالية

Key words: Trichomonas vaginalis --- culture --- orange leaves.

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