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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2005 المجلد: 4 العدد: 1

Article
التقنيات الإحيائية الزراعية والعالم العربي

المؤلفون: حسين فاضل الربيعي
الصفحات: 1-15
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الخلاصة

ABSTRACT It is of essential and importance ,the presence and development of a harmony between the need to increase agricultural and food production and the continuity in scientific innovation, especially in the field of agrobiotechnology to meet food demands and preserve our agro-ecosystem. This study deal with many related topics: The characteristic of biotechnology ,how to make use of the resources available in the ArabWorld, development of the conventional biotechnology and how will it affect the sustainability of agricultural and food systems, and finally the rules of Arab research centers in production and inhabitation of agro-biotechnology and spread it in the ArabWorld . PDF created

الكلمات الدلالية


Article
APPROACH FOR PREPARATION OF RADIATION ATTENUATED EIMENIA TENELLA VACCINE IN BROILER AGAINST COCCIDIOSIS
التوصل إلى تحضير لقاح ضد داء الاكريات في دجاج فروج اللحم باستعمال أكياس البيض المضعفة بأشعة كاما

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الخلاصة

ABSTRACT The aim of this research was to standardize the locally prepared vaccine against coccidiosis . The mature oocysts of Eimenia tenella were attenuated by gamma – radiation (200 gray) that did not affect sporozoits excystation . A one – day old male broilers were used which vaccinated by oral administration with two different doses (5×10 3 and 3×103 oocyst / chick ) at 14th day old .The clinical signs , mortalities , weight gains, pathological lesions, packed cell volume and number of oocyst in cecal content were examined . In order to examine the efficacy of vaccine to produce a protective immunity, the vaccinated groups were challenged on the 20th day post vaccination , with virulent E. tenella strain at dose of (5×104 oocysts) . This experiment demonstrated high levels of protection (99%) against the disease specially with 3×103 oocysts /chick . This vaccination program gave good weight gains without any clinical signs till marketing compared with infected group

الكلمات الدلالية

Vaccine --- Eimenia tenella --- Broiler.


Article
THE PHYSIOLOGICAL EFFECT OF GONADOTROPINS FOR INDUCTION OF SUPEROVULATION AND IN VITRO OOCYTE MATURATION AND FERTILIZATION IN MICE
التأثير الفسيولوجي للهرمونات الموجهة للقند في استحداث افراط الأباضة وانضاج البويضات واخصابها خارج الجسم في الفئران

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الخلاصة

ABSTRACT It was considered that the selection of appropriate superovulation program and in vitro maturation (IVM) by using culture media has important steps for successful of assisted reproductive techniques for medical or economical or research purposes. Therefore, this study was designed to: 1- Determination the best superovulation program in mice, and 2- Determination the effect of using of culture media on IVM and in vitro fertilization (IVF) and embryonic development later in mice. Four hundred and twenty females were divided into seven groups and injected with (10 or 15 or 30) IU dose of either pregnant mare serum gonadotropin (PMSG) or human menopausal gonadotropin (hMG), in addition to control group was injected with 0.2 ml of normal saline. Also, natural mating was achieved for fifty superovulated and fifty non superovulated females. Furthermore, nine hundred and ninety five oocytes were incubated within TCM-199 and RPMI-1640 for IVM. Then, matured oocytes and active spermatozoa were incubated withinMEM for IVF. Results of superovulation programs, number of births, percentages of IVM and IVF were registered. Results of the present study appeared that the injection of PMSG has results better than the hMG. Also, injection of dose 10 IU has results better than the doses 15 IU and 30 IU. Non significant differences in the number of births between superovulated and non superovulated females were reported. Also, insignificant differences in IVM between TCM-199 and RPMI-1640 were noticed. It was observed that the MEM has good ability for IVF. From the results of this study, it was concluded that the injectionof 10 IU of PMSGfor female mouse was effective to induce superovulaton. Also, the importance to use culture medium MEM for IVF of oocytes matured in vitro using culture media TCM-199 and RPMI-1640.

الكلمات الدلالية

Fertilization --- Superovulation Program --- Gonadotropins


Article
STUDY THE OPTIMAL CULTURAL CONDITIONS FOR ALGINIC ACID PRODUCTION BY LOCAL ISOLATE OF AZOTOBACTER VINELANDII USING SOLID STATE FERMENTATION
دراسة الظروف المزرعية المثلى لانتاج حامض الالجنيك من عزلة محلية بوساطة تخمرات الحالة الصلبة . Azotobacter vinelandii لبكتريا

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الخلاصة

ABSTRACT Five alginic acid producing isolates of Azotobacter vinelandii were obtained from local soil samples. One isolate, designated as isolate No.1,was selected for the present study , based on it,s high productivity to alginic acid among the others when grown on agar media. Wheat bran was shown to be a supporting substance for alginic acid production by isolate No.1 using solid suitable state fermentation system. Some optimal cultural conditions for alginic acid production by solid state fermentation were determined. These included , the moisture ratio, kind and concentration of carbon and nitrogen sources, and incubation period. It has been shown that the highest amount of alginic acid was recorded , when wheat bran was moistened with a liquid medium containing 4 % Zahdi date extract , as carbon source and 0.75% baker yeast as nitrogen source, the moisture ratio was 5:1 (ml:g wheat bran), and the incubation period was 6 days at 28 ºC. _______________________________________________________

الكلمات الدلالية

Azotobacter --- Alginic acid --- Solid State Fermentation


Article
THE EFFECT OF PULSED NITROGEN LASER ON THE ELONGATION OF E.COLI MM294 AND pRP4 CONJUGABILITY
دراسة تأثير ليزر النتروجين النبضي على تطاول السلالة E.coli MM294 PRP وعلى القابلية الاقترانية للبلازميد 4للبلازميد

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الخلاصة

ABSTRACT This study analysed the effect of pulsed nitrogen laser: 337.1 nm wave length , 1.5 millijoule pulse energy,10 nanosecond pulse width and pulse repetition rate range from (1-50) pulse/ second on the viability of E.coli . Results indicated that the irradiation of E.coli MM294 containing pBR322 with repetition rate (8,16,32) pulse /sec for (1,5,10,25) min , respectively ,led to significant decrease in cell number proportional to the exposure time and number of pulses per second accompanied with elongation of some cells as expression for their sensitivity to UV laser radiation .The effect of N2- Laser on the conjugability of the resistance plasmid pULB113 was also studied . the plasmid carries resistant genes for Ampicillin , Tetracycline and Kanamycine sulphate. It also carries the transposon mini Mu ( pRP4::mini Mu). In this respect E.coli JMP39 containing pULB 113 was exposed to pulsed N2 laser for 20 min with pulse repetition rate of 30 pulse/ sec . The strain was then used as a donor in mating experiments with E.coli HB101 . The Millipore filters technique revealed that non irradiated transconjugants were grown on plates selective for the antibiotic markers with high frequency( 1.33 x 10ˉ³ ) but irradiated transconjugants were grown at much lower frequency (1x10¯5) which may indicates inactivation of the cells and mutation in the tra- genes.

الكلمات الدلالية

Keywords: Laser --- Plasmid --- Conjugation


Article
PRUIFICATION OF DNASE PRODUCED BY LOCAL STREPTOMYCES ISOLATES
تنقية الانزيم الهاضم للحامض النووي DNA المنتج من بكتريا الستربتومايسس المحلية

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الخلاصة

ABSTRACT The DNase enzyme which produced from local (Streptomyces sp.SH-10) was purified by fractionation by aqueous two phase system followed gel filtration through Sephacryl S-200 by two steps. The final purification folds was 4.33and the yield of the enzyme was 28.8% . The molecular weight of the purified enzyme determined by gel filtration was 19950, The optimum pH for enzyme activity and stability were 7.0 and 6.5-7.5 respectively, and the optimum temperature for enzyme activity was 35oC . ___________________________________________________

الكلمات الدلالية

DNase --- Purification --- Streptomyces --- Two phase phase


Article
PRODUCTION OF YEAST EXTRACT FROM SPENT BREWER’S YEAST
إنتاج مستخلص الخميرة من خمائر البيرة المستهلكة

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الخلاصة

ABSTRACT The production of yeast extract was studied because of its importance in the process of preparing and producing the culture media which used in medical and microbiological laboratories. The raw material used to produce this material is the spent brewer’s yeast that always thrown in the river and causes the contamination, therefore the purpose of using spent brewer’s yeast is to remove the contamination and to produce an important material for preparing the culture media. Ibnulbitar Center and the Central Public Health Laboratory checked the quality of the yeast extract and the results was accepted. A basic design of a pilot plant was done. The design includes selection of equipment, process flow diagram (PFD), process instrument diagram (PID). Process of production yeast extract was extremely economical because the raw material was available and production running cost is nearly nil, on the other hand the cost of building the pilot plant was very cheap in comparison with importing price of this material, The residue of yeast grinding and was used as a forage.

الكلمات الدلالية

Yeast extract --- Spent brewer’s yeast --- Yeast biotechnology


Article
OPTIMUM CULTURAL CONDITIONS FOR THE PRODUCTION OF ANTIMALARIAL AGENT BY STREPTOMYCES ISOLATES
الظروف المزرعية المثلى لانتاج المادة المضادة لطفيلي البرداء المعزولة محليا Streptomyces من بكتريا

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الخلاصة

ABSTRACT Twenty Six local isolates of Streptomyces spp. were screened for their antimalarial activity . Four isolates showed activity against P.vivax , Streptomyces sp.1 have the highest activity against all parasite (P.vivax) stages during 48 hours in compareson with other isolates, the inhibition percentage was 88.38%. Optimum cultural conditions for production of antimalarial agent by Streptomyces sp1 were studied. The maximum production of antimalarial agent was obtained by using modified (S) medium, which consist of 1.0% starch (carbon source), 1.0% broad bean powder (nitrogen source), 1.0% NaCl , pH 7.5, inoculum volume 3%, and incubated at 30 ° C with shaking (150 rpm) for 5 days. _________________________________________________________________

الكلمات الدلالية

Keywords:Cultural conditions --- Antimalarial agent --- Streptomyces


Article
GROUPING CHICKENS INTO RESISTANT AND SENSITIVE TO STRESS ON THE BASES OF HETEROPHIL/LYMPHOCYTE RATIO FOR GENETIC AND IMMUNOLOGICAL STUDIES.
توزيع الدجاج الى مقاوم وحساس للاجهاد على اساس دليل الخلايا في الدراسات الوراثية والمناعية H/L المتغايرة الى اللمفية

المؤلفون: وليد خضير المراني
الصفحات: 144-150
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الخلاصة

ABSTRACT 1- The Heterophil/Lymphocyte ratio (H/L) was used to separate chickens into Resistant (R) and Sensitive (S) to S.typhimurium, using the 99% lower confidence limit for selection. 2- Five groups of chickens were constructed, 20 each, varying the number of R and S in each group, from all R, (15R+5S), (10R+10S), (5R+15S) and all S. Mean H/L ratio, before and 9 day post Salmonella typhimurium infection were compared. 3- Difference in H/L ratio group means, within "before" and "after" infection were significant due to different constructions. 4- Comparison of "before and "after" H/L mean, within each group, showed a different outcome according to group construction. 5- It was concluded that individuals should be separated into R and S, using the H/L ratio as an indicator and criterion for selection for resistance before grouping the chickens for genetical and immunological studies


Article
IDENTIFICATION AND CHARACTERIZATION OF S-LAYER PROTEINS IN AROMONAS HYDROPHILA AND LACTOBACILLUS ACIDOPHILUS
توصيف و التعرف على طبقة البروتين السطحية في بكتريا Aeromonas hydrophilaِِِ وبكتريا Lactobacillus acidophilus

المؤلفون: بشرى الامين
الصفحات: 151-169
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الخلاصة

Lyophilization of Aromonas hydrophila and Lactobacillus acidophilus destabilized the H-bonds involved in binding of surface layer protein (S-layer) to the cell wall, glycerol but not water protected the bonds and prevented the loss of this layer. S-layer was extracted and purified by gel filtration on Sephadex G-100, only a single band was observed on polyacrylamide gel electrophoresis under non denaturing conditions. The molecular weight of the purified protein were estimated by Sodium dodecyle sulfate polyacrylamide gel electrophoresis, it was about 52000 dalton for A. Hydrophila and 43000 dalton for L. acidophilus. The transmission electron microscopy (TEM) showed that the glycerol protected this layer but not water.

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