Table of content

Jornal of Biotechnology Research Center

مجلة مركز بحوث التقنيات الاحيائية

ISSN: 18151140
Publisher: Al-Nahrain University
Faculty: Presidency of the university or centers
Language: Arabic and English

This journal is Open Access

About

The Biotechnology Research Center (BRC) in a Al-Nahrain University issued in 2007 the first edition of a tightly seasonal scientific journal named as the journal Biotechnology Research Center (JBRC) which got authorization in 2005 and held an impact number ISSN: 1815-1140.The journal accepts scientific researches in Arabic and English.
JBRC’s main interest is Biotechnology researches in the Medical, Molecular, Agriculture and environmental fields which have an important impact on the public and private sectors in Iraq.
JBRC’s structure consists of editing committee (headed by the manger of the BRC and the editor in chief), consulting committee (contains a well- known iraqi scientists in biotechnology) and editing secretary.
All researches are submitted to the JBRC’s regulations which is mainly is that they must be according to the journal directions and instructions , then the researches will be evaluated by three well-known scientists in the field and after that reviewing occurred by the editing committee to ensure and verify all JBRC’s instructions and regulations are taken into consideration .
A special edition of JBCR is issued to cover all researches that presented to the BRC’s scientific conferences which subjected to all regulations and instructions of publishing in JBCR.

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Contact info

brcn2012@yahoo.comيتم الاتصال عبر البريد الالكتروني لمجلة مركز بحوث التقنيات الاحيائية


0096407707766148او للاتصال تلفونيا
بسكرتارية التحرير
م.م. سعاد محمد مجيدب
محمد منير حسين

Table of content: 2008 volume:2 issue:1

Article
Study the effect of Trigonella Foenum-Graecum Foenugreek on some parameters of sperm and serum in Alloxan diabetic mice
دراسة تأثير مستخلص نبات الحلبة على بعض قياسات الحيامن والمصل في الفئران المستحدثة داء السكري بالالوكزان

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Abstract

The influence of fenugreek injected (IP) on male fertility by using following tests (measurement of testosterone level, sperm viability, activity, motility and abnormalities) and on GOT, GPT, Al.ph. and Lipid profile status was studied in normal and alloxan diabetic mice. Alloxan diabetic mice were injected with Trigonella extract for 5 weeks at a dosage of 2g/kg body weight. The Alloxan diabetic mice exhibited enhanced decrease significantly in glucose level in serum. Increased testosterone level and decreased dead sperms, abnormalities and increase motility , decrease in GOT, GPT, AL.PH. and lipid profile, in alloxan diabetic mice

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Article
Using of Corn Gluten Growing of Trichoderma harzianum and production of acid protease
استخدام كلوتين الذرة كوسط ملائم لتنمية الفطر Trichoderma harzianum وانتاج انزيم االبروتييز الحامضي

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Abstract

The study was conducted to evaluate the utilization of corn cluten which is one of the agricultural wast products "especially the insoluble solid phase" for the first time as a culturing medium for Trichoderma harzianum and production of acid protease enzyme by solid state fermentation.

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Article
Effect of China berry crude extracts on broad mites in vivo and in vitro
تاثير المستخلصات النباتية الخام لنبات السبحبح على الحَلَمْ ذو الرسغ الشعري متعدد العوائل Polyphagotarsonemus latus خارج وداخل الجسم الحي

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Abstract

Tissue culture were established from leaf and stem of china berry (Melia azedarach ) tree . Using MS media the best regulator to form callus were 6mg/l BAP, all other concentrations did not give callus . The crude extracts from leaves and callus established from leaves were extracted with water and ethanol with different concentrations. In ethanol extracts the least concentration 0.0001 half of the treated parasites were killed in 24 hours while the number increase as the concentration increase . However in callus the ethanol extracts were much higher about 8.5 were killed in the above concentration . In water extracts the least concentration 0.0001 killed half of the treated parasites in 24 hours .This number was increased 8 or 9 in 48 and 72 hours respectively . These results give us preliminary idea about the biological control of this dangerous parasite.


Article
Effects of foliar application of dried yeast solution on population density of broad mite, Polyphagotarsonemus latus (Banks) on potato
تأثير الرش بمحلول خميرة الخبز الجافة في كثافة الحلم ذو الرسغ الشعري Polyphagotarsonemus latus (Banks) على نباتات البطاطا

Authors: لؤي قحطان العاني
Pages: 17-23
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Abstract

This study were conducted at the fields of College of Agriculture in Abu-Ghraib, to investigate the effects of foliar application with different concentrations of dried bread yeast on the population density of the broad mite, P. latus on potato plants, cultivar (Desari) during the fall seasons of 2006-2007. potato plants were treated at the age of 4-5 true leaves then the application was repeated twice intervals of ten days. Results indicated that the numbers of eggs, larva and adult were significantly different in treated plants. The number of broad mite in 4th week of plant age was 3.66, 6.00, 7.33 eggs, larva, adult / leaf respectively moreover, the density was significantly increased with the other yeast treatment. The higher density for eggs and adults was in the 10th week of plant egg however, in the 8th week the higher density was 17.33 larva / leaf in the concentration 8 g/L. In the 14th week the density was reduced as the

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Article
Using Lactobacillus as Probiotic to Inhibit Growth and Adhesion of Proteus mirabilis Causing Urinary Tract Infection
استخدام بكتيريا Lactobacillus ك probiotic لمنع نمو و التصاق بكتيريا Proteus mirabilis المسببة لالتهاب المجاري البولية.

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Abstract

The aim of the study was to use lactic acid bacteria (LAB), as probiotic, to treat growth and adhesion property of Proteus mirabilis isolated from patients suffering from urinary tract infection (UTI). For this purpose, one P. mirabilis isolate (P.M.9) was selected out of 9 isolates obtained from 150 urine specimens. Due to its resistance to 11 antibiotics tested, this isolate was treated with three-fold concentrated filtrates of two lactobacillus isolates (as probiotic). Results after treatment, showed that the filtrates exhibited significant inhibitory effect against the pathogenic P.M.9 and its adhesion property especially when only an average of 3-10 bacteria /cell were adhered to each epithelial cell compared to 44-55 bacteria/cell.

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Article
Field study on the effect of the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuil. On the cotton leaf worm Spodoptera littoralis (Boisd.) on potato plant.
دراسة حقلية حول تأثير الفطر Beauveria bassiana ( Bals.) Vuil. في دودة ورق القطن Spodoptera littoralis ( Boisd. ) على نبات البطاطا

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Abstract

A field study were conducted to evaluate the efficacy of the entomopathogenic fungi Beauveria bassiana as abiocontrol agent for the cotton leaf worm Spodoptera littoralis infesting potato plants. Results indicated that soil treatment of the entomopathogenic fungi significantly reduced the percentage of plant infestation to 64.3, 60.7 % after 14, 21 days. Results of the infection intensity also significantly reduced in treatment of entomopathogenic fungi in soil to 18.7, 9.9 pore/leaf after 14, 21 days of treatment . The results revealed the higher efficiency of entomopathogenic fungi on plant yield, it become 633.3 gm in entomopathogenic fungi in soil treatment.


Article
Histopathological study on the antifungal activity of Salix plant extract on some dermatophytes
دراسة مرضية نسيجية لفعالية مستخلص نبات الSalix المضاد للفطريات على بعض الامراض الجلدية الفطرية

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Abstract

The study was designed to determine the activity of methanolic Salix extract as an antifungal agent. Rabbits were divided into three groups. The first group represents the control (6) while the second and the third groups were infected with fungal inoculums of the rabbit skin. After development of skin lesion, the infected area of the second group was treated with Salix extract until healing. The histopathological study revealed recovery from infection in the infected animals, which manifested by well-developed epithelial tissue similar to the control group.

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Article
In vitro Callus induction and Shoot proliferation in Jojoba Simmondsia chinesis (Link) Schn.
تحفيز تكوين الكالس وظهور النموات الخضرية في نبات الهوهوبا Simmondsia chinesis (Link) Schn. خارج الجسم الحي in vitro

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Abstract

From economic site;jojoba Simmondsia chinesis (Link) Schn. is very important shrub as its seeds contain high percent of oily wax . This wax composes natural and chemical charachteristics which makes shrub to be involved in many trade, manufacture and medical uses. In this study several interactions between auxins and cytokinins and gibbrellin were implemented to find out the best concentration for callus induction, vegetative growth and differentiation for experiments. BA at(2 or 5) mg/l, NAA at (0.018, 2, 2, 5) mg/l and GA3 at (0.1 or 0.2) mg/l were used. Results showed that highest percent for callus induction were (50 and 70) % respectively in the treatment (2) mg/l BA and (2) mg/l for both BA and NAA. The results revealed that using Kin. plus NAA with (0.1 or 0.2) mg/l GA3, was the best concentration lead to high percent of differentiation for vegetative shoot were (40 and 44) % respectively However, the length of vegetative shoots were (2 and 2.5) cm respectively. , this indicates the importance of the balance between these phytohormones required for shoot growth.

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Article
Aetiological agent in community acquired pneumonia in patients requiring hospitalization
المسببات الجرثومية لذات الرئة المكتسب من المجتمع للمرضى الداخلين للمستشفى

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Abstract

This study was carried out to determine the etiological agents in patients with community-acquired pneumonia (CAP). Ninety-three patients with radiologically confirmed pneumonia admitted to the Medical City, Baghdad Teaching Hospital through the period extended from October 2001 till March 2002. Also to investigate a possible correlation between etiological agents in patients with CAP and comorbid factors including age. The etiological agents were identified from 50 (53.8%) patients with CAP, while no agents were detected in 43 (46.2%) patients. Blood samples were taken from all patients for identification of Chlamydia pneumoniae and Mycoplasma pneumoniae infection. Sputum samples for culture were taken from 76 (81.7%) patients; bacterial species were isolated from 22 (23.7%) samples. Streptococcus pneumoniae was the most frequent typical bacterial pathogen isolated from 17 (18.3%) patients. Atypical pathogens (C. pneumoniae and M. pneumoniae) using ELISA technique were identified in 22 (23.6%) and 20 (21.5%) respectively. Atypical pathogens were a most common causes of CAP identified from (39.8%) cases. Sixteen (17.2%) of the patients had mixed infections (two pathogens were identified in 12 (75%) and three pathogens in 4 (25%) patients). C. pneumoniae and S. pneumoniae were the most common mixed organisms found in 6 (37.5%) of patients.

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Article
Micropropagtion of two Strawberries (Fragaria anariassa L.) Cultivars
الاكثار الدقيق لصنفين من الشليك .Fragaria anariassa L.

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Abstract

Shoot tips from two strawberry (Fragaria anariassa L.) cultivars, Gabi and Edpeeren were cultured on MS medium supplemented with 0, 0.5, 1.0, 1.5 or 2.0mg /1 of BA to study their effects on number, length, fresh weight and dry weight of shoots. The study also included measuring the effect of different concentrations of IBA (0 , 0.1 , 0.3 , 0.5 or 1.0) mg / l on rooting percentage, roots number and length. This study also included the acclimatization processes for plantlets in sand and a mixture of sand and peat moss media. Results indicated that addition of BA to the nutrient medium led to a significant increase in all investigated traits in comparison with the control with obvious superiority of 2 mg /l which reflected positively on the mean number of shoots (20.0) shoots / plant, the fresh and dry weight were 1237.73 and 136.4 mg respectively, especially with Gabi cultivar, the result showed that all the levels of IBA in the nutrient medium led to 100% rooting after 6 weeks of planting of both cultivars compared with 50% of in control. It was also noticed significant increases in the roots number and shoot lengths when IBA concentration was increased in the medium. The best growth for the rooted plantlets was found to be 1:1 sand: peat moss which showed survival percentages of 80 and 90% after 4weeks of planting Erdpeern and Gabi cultivars respectively.

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Article
Bioactivity of Origanum oil on Staphylococcus aureus
الفعالية الحياتية لزيت الاوريكنوم على بكتريا المكورات العنقودية الذهبية

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Abstract

The studies revealed that plants oil possesses Antimicrobial properties, the origanum oil organized by a broad spectrum of antimicrobial activity. This oil extract from the Origanum vulgare found in medeternian mountain especially in Turkey. biological activities were studied to determine the effect of origanum oil on Staphylococcus aureus the result revealed that origanum oil were significantly inhibited the growth of the selected bacteria . The study showed that origanum oil has Bactericidal of effect. The inhibition zone (Hz) was determinant by using different concentration from oil as (0.5, 0.25, 0.125)mg/ml . Minimal inhibitory concentration (MIC) of oil was estimated by using different concentration as (1/10, 1/20, 1/30, 1/40, 1/50) µg/mL, the MIC of bacteria was 1/30.

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Article
Study the effect of aqueous extracts of Pimpinella anisum , Foeniculum vulgara and agroup of different medical herbs on growth of bacteria ( Staphylococcus aureus) isolated from Sputum of bronchiolitis patients .
دراسة تأثير المستخلص المائي لبذوراليانسون ، الشمر ومجموعة من الأعشاب الطبية في نمو بكتريا Staphylococcus aureusالمعزولة من قشع مرضى مصابين بالتهاب المجاري التنفسية

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Abstract

This study was conducted to determine the inhibitory dose of the hot and cold aqueous extracts of Pimpinella anisum (PA) , Foeniculum vulgara (FV) , and agroup of different medical herbs on growth of bacteria , Staphylococcus aureus isolated from the sputum of bronchiolitis patients . Minimum Inhibitory concentration (MIC) method was used to test the sensitivity of the bacteria toward the above extract . The hot aqueous extract of (PA)in concentration of 23.8 and 28mg/ ml gave a strong inhibitory ability on the growth of the bacteria at MIC 0.01 mg/ ml compared with the highest concentration of 47.6 mg/ml which did not inhibit the bacterial growth at MIC 0.02 mg/ml . The cold aqueous extract of PA at 125 mg /ml concentration also did not inhibit the bacterial growth up to MIC 0.12 mg / ml . This study also revealed that a concentration of 17.12 mg / ml of hot aqueous extract of FV seeds had no inhibitory effect on growth up to MIC 0.008 mg / ml . The combined hot aqueous extract of PA and FV seeds in a ratio of 1gm to 0.5 gm respectively an 1gm to 1 gm (24and 40.32mg / ml respectively ) also did not have any inhibitory effect of bacterial growth up to MIC 0.002 and 0.004 mg / ml, respectively . The results of this experiment also revealed that hot aqueous extract of similar quantities and concentrations ( 17.28 and 31.74 mg /ml ) of a mixture of several medical herbs like ( PA , FV , Anthemis nobilis , Thymus bovei ,Gum Arabic, Nigella sativa , Althaea , and Cydonia vulgaris ) did not show any inhibitory action on bacterial growth up to MIC of 0.004 and 0.007 mg / ml . It could be concluded that the inhibitory ability of the hot aqueous extract of PA seeds is the best compared to the other extracts used . The inhibition of bacterial growth may need a carefully selected medical herbs in different concentrations and combination .

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Article
The effect of garlic (Allium sativum) extract on gram positive and negative bacteria
تأثير مستخلص الثوم Allium sativumكمضاد للبكتريا الموجبة والسالبة لصبغة كرام

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Abstract

The antibacterial effect of garlic (Allium sativum) extract was evaluated by in vitro study testing the growth of various Gram-Positive and Gram-Negative bacteria. The bactericidal activity of this extract was analyzed by serial dilution in tubes. This study, found that Gram-Negative and Gram-Positive bacteria susceptible to very low garlic concentrations. On the other hand, Gram-Negative bacteria were more susceptible than Gram-Positive bacteria, the minimal bactericidal concentration of Gram-Negative bacteria was 2 mg ml-1 but minimal bactericidal concentration of Gram-Positive bacteria was 4 mg ml- that means double inhibition concentration of Gram-negative bacteria. This study suggest that garlic have inhibition effect about Gram-Positive and Gram-Negative bacteria and garlic was natural material found in Iraq and world wide with little side effect.

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Article
The possibility of using Glycyrrhiza glabra L. Callus extracts as an alternative to plant growth regulators in plant tissue culture experiments
امكانية استعمال مستخلص كالس نبات عرق السوس Glycyrrhiza glabra L. بديلأ لمنظمات النمو النباتية في المزارع النسيجية

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Abstract

Liquorice plant is considered one of the important medicinal and economical plants. It is rich with many compounds, minerals, vitamins, and even plant hormones. This research is aimed to study the possibility of using callus tissue extracts as an alternative to plant growth regulators added to the culture media. A factorial experiment was implemented to find out the appropriate combination between 2, 4-D and BA for callus induction on Liquorice node explants. It was found that a combination of 2 mg/l 2, 4-D with 2.5 mg/l BA is the best one for callus induction and maintenance using MS medium. Water and alcoholic extracts were prepared from callus tissue at concentrations (0, 2, 4, 6, 8 or 10) ml/l then added to culture medium as an alternative to plant growth regulators. The effect of these concentrations on growth and development of tissues and organs for some plants was studied using soya bean, potato and wheat plants for this purpose. Results showed that water extract induced shoot proliferation from potato single nodes. Both types of extracts increased soya bean callus fresh weight significantly. It was found also that water extract was more effective than alcoholic one in increasing vegetative and root parts in germinating wheat seeds.


Article
Study on the isolation and identification of Pasteurella species from farm animal and human
دراسة عن عزل وتوصيف جراثيم Pasteurella multocida من الأنسان والحيوانات الحقلية

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Abstract

This study was interested in isolation and identification of Pasteuralla species in animals and human , also studing it's sensitivity to antibiotic with comparison to it's pathogenicity for laboratory animals . Tow groups of samples were collected and investigeted, 1st group consisting of one handered thirty six samples which were collected from animals ( cow .sheep , goats , and chikens ) . 2nd group was also consisting of 136 samples that were colleeted from human ( wound , urine , and sputum ) . These samples were cultured in selective enrichement brain heart infusion broth and then in the new Pasteurella multocida selective agar medium .

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