Table of content

Jornal of Biotechnology Research Center

مجلة مركز بحوث التقنيات الاحيائية

ISSN: 18151140
Publisher: Al-Nahrain University
Faculty: Presidency of the university or centers
Language: Arabic and English

This journal is Open Access

About

The Biotechnology Research Center (BRC) in a Al-Nahrain University issued in 2007 the first edition of a tightly seasonal scientific journal named as the journal Biotechnology Research Center (JBRC) which got authorization in 2005 and held an impact number ISSN: 1815-1140.The journal accepts scientific researches in Arabic and English.
JBRC’s main interest is Biotechnology researches in the Medical, Molecular, Agriculture and environmental fields which have an important impact on the public and private sectors in Iraq.
JBRC’s structure consists of editing committee (headed by the manger of the BRC and the editor in chief), consulting committee (contains a well- known iraqi scientists in biotechnology) and editing secretary.
All researches are submitted to the JBRC’s regulations which is mainly is that they must be according to the journal directions and instructions , then the researches will be evaluated by three well-known scientists in the field and after that reviewing occurred by the editing committee to ensure and verify all JBRC’s instructions and regulations are taken into consideration .
A special edition of JBCR is issued to cover all researches that presented to the BRC’s scientific conferences which subjected to all regulations and instructions of publishing in JBCR.

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Contact info

brcn2012@yahoo.comيتم الاتصال عبر البريد الالكتروني لمجلة مركز بحوث التقنيات الاحيائية


0096407707766148او للاتصال تلفونيا
بسكرتارية التحرير
م.م. سعاد محمد مجيدب
محمد منير حسين

Table of content: 2010 volume:4 issue:2

Article
Effects of aqueous extract of Salvia on some parameters of sperms and histopathological changes in testes of mice treated with Methotrexate (MTX)
تاثير مستخلص المريميه المائي على بعض معايير الحيامن والتغيرات النسجية الامراضية لخصى الفئران المعاملة بعقار الميثوتركسيت

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Abstract

Methotrexate commonly used to treat cancer and causes reproductive damage in mice. The aim of this research is to study the effects of salvia aqueous extract on some parameters of sperms and histopathological changes in testis of mice treated with methotrexate. Twenty-four adult albino male mice were divided into three groups, (8 mice in each group) First group was negative control received normal saline intraperitoneally, while second group was treated with50 μg/kg, for 35 days intrapritoneally (ip). The third group was treated with methotrexate (50 μg/kg) also (ip) and extract of salvia 85 mg/kg for 35 days. The results showed significant (P<0.05) decrease in sperms motility , increased percentage of dead sperms and abnormalities of sperms in mice treated with MTX, there was significant (P<0.05) decrease in diameters of seminiferous tubules, primary spermatocytes and spermatids, increased interstitial spaces as compared with control, while in the third group result showed significant (P<0.05) increase in sperms motility , decreased percentage of dead and abnormalities of sperms and significant (P<0.05) increase in diameters of seminiferous tubules, primary spermatocytes and spermatids and decrease interstitial spaces compared with first and second groups.

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Article
Study the Biochemical active materials (Allicin, Alliin) extraction from medical plant to treat patients with atherosclerosis
دراسة التأثير البايوكيميائي لبعض المواد الفعالة (الاليسين ، الالين ) المستخلصة من النباتات الطبية في علاج مرضى تصلب الشرايين

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Abstract

Total study was concerned with the Iraqi garlic extract which should concentration of both Allicin 23.94 ppm and Alliin 17.9 ppm respectively. The highest concentration of Arginine from sixteen amino acid and is the highest concentration of vitamin (Niacin) B3 in garlic extract. The effect of some Biochemical variables for patients atherosclerosis was studied at the age between (45-65) years old using the 3 capsules a day to give a concentration 250mg/ capsule for a period of two weeks each at the level of total cholesterol, HDLc, and MDA and the effectiveness of the enzyme G.S.T. The results indicate a low level of total cholesterol, MDA and the level of effectiveness of the enzyme G.S.T. a rise in the level of HDLc.

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Article
Optical Rhizobium Growth Density Responding to different levels of Mo and K and their interaction in the Liquid Media
استجابة كثافة نمو بكتريا الرايزوبيوم الضوئية لمستويات مختلفة من Mo وK والتداخل بينهما في الوسط الغذائي السائل

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An experiment was carried out to study the effects of potassium and Molybdenum on Rhizobium growth. The objective of the experiment, which conducted under laboratory conditions was to investigate the interaction effects of using three levels of Molybdenum (0, 0.25, 2.50) mg Mo .L-1 and four levels of potassium (0, 25, 50, 100) mg K.L-1 on the optical density of Rhizobium growth in the liquid medium (broth media), which is belong to R. leguminosarum after three days of incubation at 28°C.

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Article
Establishment of HPRT and DHFR Gene Mutation Assays as biomarkers In Sheep lung fibroblasts (SLF)
فحص الطفرة الوراثية لجين الـHPRT و DHFR كمؤشرات وراثية في خلايا رئة الاغنام

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Abstract

The aim of this research is to establish a gene mutation assay for examining the integrity of animal cell genome for nuclear transfer technique. Lung fibroblasts which were expanded from 4 months old female lamb were selected as target cells. These cells were coded (SLF) as Sheep lung cells. Growth characterization, doubling time, chromosomal number and structural integrity were checked after their growth in RPMI-1640 medium. For HPRT-gene mutation assay, the cells were plated at density of 1×103cells/plate and grown in medium containing toxic concentrations of 6-thioguanine; while for DHFR -gene mutation assay, toxic concentration of methotrexate was used as a selective agent. Those cells were grown for 15 days; mutant colonies either 6TGr or MTXr were reinoculated in a selective medium for further 8 weeks for checking the stability of phenotypic expression of mutant cells. The results revealed that SLF cells has spontaneous frequencies of HPRT, and DHFR gene mutations equal to 16 and 22×10-3 event/ generation/ cell, respectivel.These levels are normal comparing with other animal cell types, and these assays could be applied on other somatic cells.

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Article
Identifcation of some local wheat varieties by high performance liquid chromatography technique
تمييز بعض أصناف الحنطة الناعمة المحلية باستخدام جهاز الفصل الكروموتوكرافي السائل عالي الاداء

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Gliadin protein of three soft wheat cultivars namely Abu-Ghraib, IPA 99 and AL-Littefia was extracted and separation by high performance liquid chromatography HPLC. Result showed that there were significant differences between the gliadin percentage ratio with the order of AL-Littefia 34.02% , IPA99 30.20% and Abu-Ghraib 31.00% . Also the result of HPLC analysis revealed that the gliadin of the test cultivars has 3 distinguish patterns of separation. Thus it appears that HPLC provides efficient tool for identification of wheat cultivars. Abu-Ghraib gliadin showed 6 peaks while IPA99 and AL-Littefia showed 15 and10 peaks respectively.

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Article
Effect of Colocynthis (Citrillus colocynthis) plant extract on leukemic cells
دراسة تأثير نبات الحنظل على الخلايا السرطانية

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Abstract

The present research concentrated on evaluating the biological activity of Colocynthis (Citrillus colocynthis) plant oil extract on peripheral blood cells from leukemic patients using some cytogenetic parameters. when it is added to this cell in culture media in different concentration and incubated for 72 hours at 37C, the results pointed out that cell exposed to the Citrillus extract in culture media have mitotic index near to normal ranges, and number of chromosomal aberrations in cells exposed to the Citrillus extract is less than in the leukemic cells. This study considered as an attempt in survey for anti-tumor agents in medicinal plants.

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Article
Purification and Characterization of Nitrate Reductase (NAR) from Pseudomonas sp. SH7 Isolate
تنقية وتوصيف انزيمNitrate Reductase (NAR) المنتج من بكتيرياPseudomonas sp. العزلة SH7

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Abstract

Sixty five soil samples and fifteen water samples were collected from different places in which previously explosions were occurred in Iraq. Seven isolates showed ability to utilize 0.1mM trinitrotoluene (TNT) and/or 0.2mM glycerol trinitrate (GTN) as a sole carbon and nitrogen source and one of these isolates showed the highest nitrate reduction which was classified and coded as Pseudomonas sp. SH7. The highest nitrate reductase activity extracted by sonication while optimum conditions for enzyme production in minimal media pH 7 containing 0.25mM GTN at 35oC for 3 days under aerobic condition. Nitrate reductase was purified by 40-60% ammonium sulphate, ion exchange and gel filtration. Nitrate reductase molecular weight determined by SDS-PAGE was 115 kD. The characterization of purified enzyme activity and stability was higher at a pH between 6.5-7.5 and. Maximum activity was at 35oC and stable at 30-40oC for 15 min., while for heat sensitivity 100% activity observed at 45oC for 20 min. Treatment with 200 µM azide and 500 µM cyanide inhibited the activity by 76 and 91% respectively.


Article
Investigation of the Antimicrobial Activity of Some Streptomyces spp. Isolated from Nineveh Province
التحري عن الفعالية المضادة للجراثيم لبعض عزلات Streptomyces المعزولة من محافظة نينوى

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Abstract

Atotal of 62 different Streptomyces isolates were recovered from 17 samples of soil collected from different sites of Nineveh Province. Only 23 isolates showed activity against test Gram-positive and Gram-negative bacteria. One isolate showed high antimicrobial activity against Staphylococcus aureus, was selected and identified as Streptomyces lavendulae on the bases of microscopic, morphologic, biochemical tests and its sensitivity to some antibiotics. The bioactive compound produced by S. lavendulae was isolated on TLC plate (Rf 0.85). The UV spectrum of the active compound in methanol showed one peak at 280 nm. From these data it could be concluded that the active compound probably belongs to macrolide antibiotics group.


Article
Study of some Cytogenetic effect of licorice extracts (Glycyrrhiza glabra) on blood lymphocytes of Awassi ewes and embryos
دراسة تاثير بعض مستخلصات عرق السوس ( Glycyrrhiza glabra ) الخلوية الوراثية في خلايا الدم اللمفاوية للنعاج العواسية وخلايا سلي اجنتها

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The effect of licorice extract [Glycyrrhiza glabra] on somatic cells chromosomes from 30 Awassi ewes (local breed) before and after pregnancy to improve soil fertility through the use of chromosome analysis of lymph blood cells and on their embryos from amniotic cells chromosome which drain by laparotomy of the maternal and fetal. That is the aim of this study. The blood taken from ewes treatment [0.5ml] for each sample incubate in media Tc-199 with Brdu for 72 hours worked in Cytogenetic analysis of the models Rd cells of embryos derived from the total ewes experience a way to open the abdomen of the pregnant ewes )12-13( weeks to demonstrate the effects of licorice extract chromosomes in blood lymphatic cells of mothers and fetuses after treatment. Cytogenetic analysis revealed that licorice extract could not induce numerical nor structural abnormalities in chromosomes from ewes blood lymphocytes, after treatment or on their embryos from amniotic cells chromosomes which drain by laparotomy under their experimental designs.

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Article
Synthesis and Antibacterial Studies of Novel Phosphorus Ligand 5-(2-diphenylphosphino) phenyl-1,2-dihydro-1,2,4-triazole-3-thione and its Metal Complexes with some Transition Metals
تحضير ودراسة الفعالية الحيوية لمركب الفوسفور الجديد 5-(2- ثنائي فنيل فوسفينو) فنيل-12,-ثنائي هيدرو-4,2,1-ترايازول-3-ثايون ومعقداته مع بعض العناصر الإنتقالية

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This study involves the chemical synthesis of the novel ligand 5-(2-diphenylphosphino) phenyl-1,2-dihydro-1,2,4-triazole-3-thione (DPDTT) by the reaction of 2-diphenylphosphino benzoic acid with absolute ethanol that yield ethyl 2-diphenylphosphino benzoate and by cyclization of this compound with thiosemicarbazide, DPDTT will be produced. The chelating complexes of this ligand with Cr(III), Co(II), Ni(II), Cu(II) and Cd(II) were also prepared and studied. The new complexes were characterized by FT-IR, UV/visible spectra, and room temperature magnetic susceptibility. The stability for the prepared complexes was also measured using the density function theory and it was found that the cadmium complex is the most stable and the chromium complex is the least stable. Free ligand and its metal complexes have been tested in vitro against a number of microorganisms, like gram positive bacteria Staphylococcus aureus and gram negative bacteria E. coli, Proteus vulgaris, Pseudomonas and Klebsiella in order to assess their antimicrobial properties. All complexes showed considerable activity against all the studied bacteria.

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Article
Evaluation of antioxidant activity for pure curcumin in albino mice
تقييم الفعالية المضادة للأكسدة لمركب الكركمين النقي في الفئران البيض

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he study was carried out in order to determine the toxic, oxidant and antioxidant effects for one compounds of the Curcrcuma longa L. in comparison with vitamin C against the mutagenic effect of carbon tetrachloride CCl4, which is a chemical compound that damages hepatic cells and has mutagenic effects. The effect was studied in a mammalian system. This in vivo system depended on evaluating the enzymatic activity of two anti oxidant enzymes: Catalase and Glutathione reductase. Two concentrations of pure curcumin 200 and 400 mg /kg were evaluated to chose the suitable concentration, which remembered the negative control. In order be used in The interaction experiments, included two types of treatments pre – CCl4 and post-CCl4 in order to determine the mechanisms of the pure curcumin in preventing or reducing the antioxidant effect of CCl4. The pure curcumin showed no toxic and oxidant effects in biological system, and instead it showed highly performance in preventing or reducing the oxidant stress effects of CCl4. It increased the Catalase and Glutathione reductase antioxidant activity, especially in dose 200 mg/kg. The positive effect was higher when pure curcumin was used as pre-CCl4 treatments, and to less extent in post-CCl4 treatment. Therefore, the plant extracts can be considered as adesmutagen at the first degree and a bioantimutagen at the second degree.

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Article
Influence of sucrose and tyrosine on callus induction and morphine and codeine production on Papaver somniferum In vitro
تأثير السكروز والتايروسين في استحثاث الكالس وانتاج المورفين والكودائين من نبات الخشخاش Papaver somniferum خارج الجسم الحي

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Abstract

A study was conducted at the tissue culture lab. College of Agriculture/ University of Baghdad and Drug Research Center/ Health Minstry during 2006 - 2007. Results indicated that MS medium supplemented with 30 g/l of sucrose gave the highest values (630.3, 61.6) mg of both fresh and dry weight of callus respectively. However, values of both parameters were reduced with increasing sucrose concentrations up to 60, 90, 120 g/l (478.2, 346.5, 217.6) mg respectively. MS medium supplemented with 30 mg/l of tyrosine gave the highest values of callus fresh and dry weight (429.7, 42.6) mg respectively. MS medium supplemented with 30 g/l of sucrose and 30 mg/L tyrosine gave the highest values (688.0, 67.5) mg of both fresh and dry weight callus. MS medium supplemented with 30 mg/l of tyrosine and 90 g/l of sucrose gave the highest values (2.9, 2.8) mg/g of morphine and codeine from fresh callus weight respectively while the lowest values of morphine and codeine (0.5K 0.2) mg/g from fresh callus weight respectively of control treatment .

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Article
Influence of various levels of L-asparaginase II purification on the cytotoxicity, DNA level, and apoptosis in Hep-2 cells
تأثيرالمستويات المختلفة من التنقية لأنزيم L–asparaginase II في السمية الخلوية ، مستوى إلـ DNA وعملية الموت المبرمج للخلايا السرطانية Hep–2

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The genetic effects of several concentrations of L–Asparaginase II (ASNase II), produced by Proteus vulgaris strain Pv.U92, at various levels of purification (ultrasonication, precipitation, ion-exchange chromatography and gel filtration chromatography) on cancer cells line of Hep–2 were studied. This bacterial enzyme with concentration 4 U/ml at gel filtration level was revealed a putative cytotoxicity against cancer cells in comparison with other concentrations and steps of purification were used in this work. Moreover, 4 U/ml of ASNase II at gel purification level has a distinguished role on arrest cancer cells division of Hep–2; it was reduced the content of DNA at each phase of cancer cell cycle particularly at G2/M phase, the level of DNA was 3%. On the other hand, the partial purified enzyme, L–ASNase II, was induced apoptosis by both levels of purification ion–exchange and gel filtration, the apoptotic fractionation was 0.86 and 0.7 respectively .


Article
In vivo and in vitro Study of Probiotic Effect of Lactobacillus acidophilus on Pathogenecity of Proteus mirabilis isolated from Urinary Tract Infection (UTI)
دراسة تاثير الراشح الطبيعي المستخلص من بكتيريا اللبن على امراضية بكتيريا المتقلبات المعزولة من التهاب المجاري البولية

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The aim of the study was to treat the pathogenesity and adhesion property of Proteus mirabilis isolate, obtained from urinary tract infection (UTI) patients. After the identification of Proteus mirabilis it was found that the pathogenic bacteria possessed the adhesion ability both in vivo and in vitro bioassays, with lactobacillus acidophilus filtrate as Probiotic. Proteus mirabilis was subjected to different concentrations of lactobacillus acidophilus filtrate to investigate its adhesion property and pathogenesity. Three concentrations (25, 50,100) % of Lactobacillus acidophilus concentrated filtrate were used in vitro bioassays against pathogenic bacteria. Results showed that the third fold 25% was the most effective concentration in reducing the adhesion intensity of the bacteria. This concentration was selected to be used in in vivo for detection of infectivity to the animal tissues (Kidney and liver) and also the effect of Proteus mirabilis and Probiotic on the level of hormones in liver; Serum glutamate pyruvate transaminase, Serum glutamate oxoloacelate transaminase and Alkaline phosphtases (GPT, GOT , ALP) and kidney enzyme (Urea) in serum of mice was investigated. It was found that the levels of liver hormones and kidney enzyme increased at the time of infection, and these levels returned to normal or decrease after treating the animals with 25% of concentrated filtrate of Probiotic.

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Article
Effect of some Transition Metals and Selenium on germination of Eruca sativa Seeds
تأثير بعض العناصر الانتقالية والسيلينوم في انبات بذور الجرجير Eruca sativa

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The effect of some transition metals, Nickel, Copper, Zinc and Selenium elements on Eruca sativa seeds germination was studied. Concentration (1, 10, 100, 500, 1000) ppm in sterile distilled water were used to cover the natural concentrations and the concentrations in polluted environments. Germination was recorded after 5 days. Results showed that there were inverse relationships between the number of germinated seeds and increasing in concentration of elements. The effect was mainly due to the elements, while the pH effect was very low and not exceeds 7 % except for Zinc solution which was about 50%. The correlation coefficient between germination and concentration were with negative values, it was for Nickel element -0.842, Copper -0.965, Zinc -0.928 and for Selenium -0.603. The values were statically significant except for selenium. According to the obtained results the elements can be ranked Nickel the less effect < Copper < Zinc < Selenium.

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Article
Effect of Laser on Some Biological Performance of Ephestia cautella Walk
تأثير الليزر في بعض جوانب الأداء الحياتي لحشرة عثة التين Ephestia cautella Walk

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he effect of different doses of laser on some biological performance of Ephestia cautella exposed as larvae of 5- 6 days old and pupae of 1 , 5 days old were studied. Both lasers have significant effect on the larvae, pupae period emerged adults emerged and life span of emerged adult. Furthermore the number of eggs laid and its percent of hatch were investigated for different mating types.

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Article
Molecular diagnosis of Iraqi chronic myeloid leukemia patients using quantitative real-time PCR
التشخيص الجزيئي لمرضى سرطان الدم النخاعي المزمن في العراق

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hronic myeloid leukemia (CML), also known as chronic granulocytic leukemia, is a form of leukemia characterized by the increased and unregulated growth of myeloid cells in the bone marrow and the accumulation of these cells in peripheral blood. Total RNA extraction, cDNA and quantitative real-time PCR (Q-RT-PCR) were done for thirty CML Iraqi patients. Hematology tests (hemoglobin, platelets and WBCs counts) were done for the same samples in the same time. The results of this study show that some samples with normal hematology values have BCR-ABL (p210) fusion transcript with molecular analysis by Q-RT-PCR. This indicates the importance of this technique in the diagnosis and monitoring the therapy of CML patients.

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Article
Preparation of shrimp shells chitosan and use it in extending eggs shelf life
تحضير الكايتوسان من قشور الروبيان واستخدامه في أطالة العمر الخزني للبيض

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This study aimed to examine the performance of chitosan prepared from chitin. By treating the shrimp shells with 3.5% sodium hydroxide then with 1 N hydrochloric acid. The chitin produced was used to prepare two types of chitosan by treating it with concentrated solution of sodium hydroxide 50% with heating at 100c°for 4 hr. (type A) and 20 hr. (type B). The molecular weights of samples A and B as determined by viscometer were 1155 and 720 KDa. The performance of the two chitosans was examined in preserving eggs quality and extending its shelf life after coating them with 2%of chitosan and keep it at 25C°for 4 week. The results indicate that both samples showed better performance than control (non-coated eggs and eggs coated with 1% acetic acid) as A and B contributed in preserving albumen and yolk quality, whereas, Hough Unit of coating sample decreased from 100 (in the 1st day) to (76.3, 76.8), respectively after 4 wk of storage while the control (non coated eggs and 1% acetic acid coated eggs) was (68.2, 67.9), respectively. The yolk index decreased from 0.6 (in 1st day) to 0.27 and 0.28 for eggs coated with chitosan A and B, respectively and to 0.21 and 0.22 for none coated and 1% acetic acid coated eggs respectively after 4 week of storage. A and B coated samples contributed in decreasing the albumen pH increasing which increased from 7.9 ( in the first day) to 10.4 and 10.8, respectively as compared to 11 for the control. B sample was the best compared to A sample and control in preventing decreasing weight losses which were (5.51, 6.12, 6.33, 7.69) %, respectively. Therefore A and B coated eggs showed high activity to improve the internal quality of eggs and to extend its storage period.

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Article
Cytogentic and bacteriological study of raw and magnetic milk
دراسة الوراثة الخلوية والميكروبية للحليب الخام والممغنط

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his study was conducted to investigate the efficiency of magnetic system in milk sterilization. This was done using bacteriological, (total microbial count) and cytogenetic (mitotic index and chromosomal aberration of bone marrow) investigations. Results indicated a reduction in total milk microbial count. On the other hand, mitotic index was increased and chromosomal aberration in bone marrow was decreased in magnetic milk as compared with raw milk. In conclusion, milk sterilization might performed using magnetic field application.

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Article
0Production of Procyanidin compound in seeds , callus and in regenerated plants from vitis vinifera L.
إنتاج المركب الفينولي Procyanidin من البذور والكالس والنباتات المتولدة من الكالس في نبات العنب Vitis vinifera L

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This experiment was conducted in Science and Agriculture College labs, Kufa University, on 2008 to describe methods for extraction and purification of Procyanidin compound from each of grape seeds mother plant(Vitis vinifera L.) ,callus induced from Axillary buds by using MS medium supplemented with 2,4-D at concentration of (1,2,3)mg/l and from leaves of regenerated from callus by using MS medium supplemented with ( IAA )at concentration of 2mg/l and (BA) benzyl adenine at concentration of 0.2 mg/l and from cocoas powdered (control) as well these Procyanidin. The content of Procyanidin were done by using HPLC (high performance liquid chromatography) and compare of the quantitatively of Procyanidin with these seeds in mother plant and callus tissue. This study include alcohol extraction by using mixture of ethanol, methanol, D.W and HCL. The result revealed that, the superiority of Procyanidin in seeds content than the content in callus and regenerated plant and increase the content of these compound in the leaves of regenerated plant with the content in callus tissue.

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Article
Partial Purification and Characterization of Inulinase Produced from a local isolate of Aspergillus niger J3
تنقية وتوصيف انزيم الانيولينيز جزئياً المنتج من العزلة المحلية للعفن Aspergillus niger J3

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Inulinase was produced from local isolate of Aspergillus niger J3. The inulinase was purified by two steps included precipitation by amonium sulphate at (30-80) % sa-turation and gel filtration on sephadex G100. The final purification folds and the yield of the enzyme were 3.15 times and 28.24%, respectively. The purified enzyme has the following characteristics: The optimum pH of the enzyme activity was 5.5. The enzyme was most stable at pH (4.5 - 6). The optimum temperature for its activity was 45c. The enzyme retained its original activity when incubates at (30-55) c for 20 minutes. Mercury chloride inhibited the enzyme completely at concentration of 10mM, cupper sulphate and calisium chloride inhibited the enzyme at concentrations of 85% and 7% respectively. It was revealed that the enzyme had the efficiency to hydrolyze 87% of 5% inulin solution when treated at 45c for 120 min.

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Article
Using PCR Technician in Prenatal Diagnosis of Fetal Gender
استخدام الـ PCR لتشخيص جنس الجنين قبل الولادة

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whole blood samples were obtained from 30 pregnant women at 15 –24 weeks of gestation. DNA was extracted from each plasma or serum sample. To detect the Y-chromosome specific marker DYS14 in the maternal blood, (Polymerase Chain Reaction) PCR were carried out for each DNA extract. The PCR products were analyzed by 1.5% agarose gel electrophoresis and ethidium bromide staining. The results compared with fetal gender after delivery. The result of delivery revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus and DYS14 was detected in all plasma and serum samples obtained from pregnant women and revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus. The PCR sensitivity for detecting the gender of fetus from maternal whole blood at 15–24 weeks of gestation was 100% in both plasma and serum, DYS14 was not detected in the DNA from any of the 17 pregnant women carrying a female fetus. The results showed that PCR analysis of maternal plasma and serum can be used to diagnose fetal gender.


Article
The Effect of different crude methanolic extracts against some gram negative pathogenic bacteria
تاثيرالمستخلصات الميثانولية الخام لبعض النباتات ضد بعض البكتريا السالبة الممرضة

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This present study was conducted to evaluated the antibacterial activity of crude extracts (Methanolic) of punica granatum peel, Menthe longifolia and Olea eurpeae leaves against some gram negative bacteria like Pseudomonas aerugenosa, Serratia marcesens, Escherichia coli, Proteus vulgaris, Klebsiella pnemunoniae. The Agar-well diffusion was used to know the ability of inhibititory of crude extracts against bacteria. Results showed different susceptibility response of bacteria under study. It was observed that Pseudomonas aerugenosa the most susceptible to Methanolic extracts to all plants in (25, 50) mg/ml-1 concentrate. While the bacterial strains shown no responding against mint and pomegranate extracts in 10 mg/ml-1 except Pseudomonas aerugenosa and Escherichia coli, was inhibited with Olive extracts only. That ability to inhabitation less in concentrate 10 mg ml-1.

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Article
Effect of some chemotherapy drugs) vincristine and vinblastine) on females albino mice fertility and sex hormones level
تاثير بعض ادوية العلاج الكيمياوي (الفنبلاستين والفنكرستين) على خصوبة اناث الفئران ومستوى الهرمونات الجنسية

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The aim of this study was to determine the effect of chemotherapy (vincristine and vinblastine) on the fertility and sex hormones level of female mice. Twenty four female albino mice were divided into three equal groups. The First group injected with vincristine 0.05 mg/kg intraperitoneally daily for 3 weeks. The second group injected with vinblastine 0.05 mg/kg intraperitoneally daily for 3 weeks. The third group treated with normal saline intraperitoneally daily for 3 weeks as control group. Blood samples were collected by using heart puncture within plastic syringes and transferred to plastic tubes. Blood centrifuged and serum was harvested for hormonal assay. The animals in each group were killed by dislocation of cervical vertebrate in third week of the treatment. Ovaries weighed and fixed with Bouin solution for histological study. Results showed significantly (P<0.05) decreased in ovaries weight on groups treated with vincristine and vinblastine as compared with control group. The mean numbers and diameters of primary, secondary follicles and corpus luteium, and hormones level [follicular stimulating hormone (FSH), lutenizing hormone (LH), estrogen and progesterone] were significant declined in groups treated with vincristine and vinblastine as compared with control group. Results indicated that fertility and sex hormones level reduced in females treated with chemotherapy drugs vincristine and vinblastine


Article
Estimation levels of Indol acetic acid (IAA) and Gibberellic acid (GA3) from dry bakery yeast Saccharomyces cereviciae
تقدير مستوى هرموني اندول حامض الخليك IAA وحامض الجبريليك GA3في خميرة الخبز العادية الجافة Saccharomyces cereviciae

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Abstract

The level of endogenous free, bound and total auxin (Indol-3-acitic acid, IAA) and gibberellic acid (GA3) were examined in dry bakery yeast. For determination of the levels of these plant growth regulators level, used spectrophotometer. The results show that Saccharomyces cereviciae was produced IAA and GA3. IAA level was 29.86 µg/ml, 198 µg/ml at 222nm and 280nm wavelengths respectively and GA3 level was 799 µg/ml at 254nm.

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Article
Immunomodulation of mice blood cells following treatment with free extract of Lactobacillus acidophilus cell
التعديل المناعي لخلايا دم الفئران البيض بعد المعاملة بالمستخلص الخالي من الخلايا لبكتريا حامض اللاكتيك التعايشية النوع Lactobacillus acidophilus

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Abstract

A study aimed to examine immunomodulatory effect of concentrated cell free filtrate (CCF) of Lactobacillus acidophilus on immune system of mice by detecting the total and absolute counts of leucocytes. It was shown that CCF of Lactobacillus acidophilus caused a significant increase in total leucocyte count for all concentrations used in comparison with negative control. However, in absolute leucocyte counts the, CCF of L.acidophilus at doses (500 , 750) mg/kg caused a significant increased in lymphocyte, neutrophil and monocyte counts, while there was no significant differences in eosinophils and basophil cells number of mice treated with bacterial filtrate. The interaction study showed that the post-treatment with CCF of L. acidophilus caused a significant increase in total leucocytes count and absolute counts of all types of leucocyte cells except basophils and esinophils which showed no significant differences in comparison with pre-treatment

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