Table of content

Kufa Journal For Veterinary Medical Sciences

مجلة الكوفة للعلوم الطبية البيطرية

ISSN: 20779798
Publisher: University of Kufa
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

About

Kufa j. vet. Sci. is a scientific and refereed journal published by the college of veterinary medicine , University of Kufa , Kufa ,Iraq. It publishes in English original and distinguished research papers , communications , research notes , case reports and reviews in all aspects of veterinary sciences .
Manuscript shall be considered for publication only with the understanding that they are neither under concurrent consideration by any journal nor have been published else where . Manuscript are subjected to peer review for their scientific merit .accepted manuscript will become the property of Kufa j . vet. Sci . the journal is published two times a year.

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Contact info

البريد الإلكتروني لرئيس التحرير
vetmed@uokufa.edu.iq
أو البريد الإلكتروني للمجلة
kj.vs@uokufa.edu.iq
أو للإستفسار الإتصال بمسؤول المجلة على الرقم 07810115211

Table of content: 2011 volume:2 issue:2

Article
Study of acariasis in cattle and ticks resistance against cypermethrin in Al-Najaf province
دراسة الإصابة بالقراد ومقاومة القراد ضد السايبرمثرين Cypermethrin في محافظة النجف

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Abstract

Abstract: The present study was carried out at September 2010 to evaluated tick infestation of cattle in Al-Najaf province with diagnosis of cypermethrin resistance by using FAO larval packet test and modified larval packet test. The result showed that the infestation rate of acariasis was 54.34%. The identification of ticks revealed that 55.06% of collected ticks were Hayaloma spp., while the percentage of Boophilus spp. and Rhipicephalus spp. Were 31.86% and 13.08% respectively. According to site of infestation, the highest percentage was recorded in udder 45%, followed by perineal region 25%, head –neck 18% and others 7%. The LPT results showed there was no significant variation (P≤0.01) between the mortality rates of Boophilus (96.42%) and Hyaloma (96.72%) at recommended dose. In other hand, the MLPT method showed that the mortality rate of Boophilus larvae at recommended and double recommended doses were 94.82% and 98.04% respectively, while all the Hyaloma larvae were killed at the recommended dose.

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Article
Comparison between Cinnamoum cassia and Ocimum basilicum on some Biochemical and blood tests Rats
دراسة مقارنة نباتي القرفة والحبق على بعض المعايير الكيموحيوية والمعايير الدموية في الجرذان

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Abstract: This study was designed to compare the effect of Cinnamoum with basil on some biochemical and blood tests in adult rats. Twenty one adult males used in this experimental divided into three equal groups: Control group (c) : which was given normal water and food. Treatment Group (F): which was given Cinnamoum with water orally (8 g / kg / B. W.) for 6 weeks. Treatment with basil (L): which was given aqueous extraction of basil orally (2 ml / kg / B. W.) for 6 weeks. Blood samples were taken direct from the heart in 2 , 4 , 6 weeks from experimental design to measure WBCs, Hb , Glucose , Cholesterol. The results of this study have appears that the Cinnamoum and basil play roles in decrease Glucose , Cholesterol of blood and necessary for diabetic disease and increase in WBCs, Hb.


Article
A study effect of age of dam and sex of birth on chemical and physical composition of milk in some farm animals
دراسة تأثير عمر الأم وجنس المولود في التركيب الكيمياوي والفيزياوي للحليب في بعض حيوانات المزرعة

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Abstract

Abstract: This study was carried out at AL-Qadisiya Governorate/ AL-Shinafia district during a period from July- September 2010 to evaluate the effect of age of dam and sex of birth on physico-chemical composition of milk in some farm animals(cows ,sheep and goats). by using ultra-sonic milk Analyzer to analysis of physico-chemical composition of milk. A total of (153) samples of milk were used in this study distributed in to (54) samples for cows, (53) samples for sheep and (46) samples for goats. These samples were collected from animals directly and kept in ice box during transportation to the laboratory to analysis. Results of this study showed that milk of sheep and goats didn’t differ significantly as compared with cows milk in (PH, Density, Solid not fat).But differed significantly (P˂0.05) in other components. The study revealed that sheep milk contain the highest Ash(0.74%) while cow milk had lactose (4.25%) , but goat milk had the highest solid not fat (8.27%) and protein (3.37%). The study showed that there was no significant effect of sex of birth on physico-chemical composition of milk of cows, sheep and goats with exception the effect of it on lactose percentage in sheep. Age of dam didn’t effect on most physico-chemical composition of milk of cow and goat and some chemical composition of sheep milk (Ash and Fat).

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Article
The Effects of Waste Products of generators exhausts on Foam Gland in Male Japanese Quail (contunix conurnix) Anatomical and Histological study
تأثير مخلفات عادم المولدات على الغدد الرغوية في ذكور طائر السلوى , دراسة تشريحية ونسجية

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Abstract

Abstract: This study had been made to detect the gross and histological changes of cloacal gland (foam gland) in Fourty males of Japanese quail which exposed to waste products of generator exhausts with different periods. The Fourty males Japanese quail were divided into four groups showed that, a mild foam production of cloacal glands with mild atrophy was observed in third group which exposed to exhaust for a period of 20 days. The cloacal glands were atrophied, and no foam production was appeared in the first and second groups which exposed to exhaust for a period of 40 and 60 days, while normal glandular size and heavy foam production was noticed in the fourth group (control group). The histological changes of cloacal gland in the first and second groups revealed atrophy of its secretory units and the lining of these secretory units were flat epithelial cells. Beside, the septa and the interstitial connective tissue were thick. The lining secretory units of the cloacal gland in the third group showed low cuboidal cells with slightly thickened of the lamina properia and the interstitial connective tissue. While the cloacal gland of control group showed features of normal glandular histology were their secretory units lined by simple columnar epithelium. These cells have negative PAS stain granules. The lamina properia in the control group was thick with well vascularized connective tissue.

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Article
Physiological Effects of Zinc Oxide in Some of Reproductive and Blood Parameters in Female Rabbits
التأثيرات الفسلجية لأوكسيد الزنك في بعض معايير الخصوبة والمعايير الدموية لدى إناث الأرانب

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Abstract

Abstract: This study was performed to identify the physiological effects produced by addition of zinc oxide at 96 mg/ kg diet for 40 days in some of reproductive and blood parameters in female rabbits. Sixty local domestic rabbits of both sexes were used (20 males and 40 females), the males separated from females while females divided into two group, first group: (20 female) represented the control, the second group: (20 female) represented the treatment group in which ZnO2 added at 96 mg/ kg diet along the experiment period which lasted from 20/4/2008 to 30/5/2008, after 10 days from the beginning of the trail, mating occur between half males number (10) with the females of the control group and the other half of males with the females of treatment group, the males selected randomly (1male:2 females), then the males removed and the following parameters counted: gestational ratio, mean period of gestation, delivery ratio, mean of the numbers of offspring, the offspring mean weights, red blood cell level, white blood cell level and hemoglobin. The results revealed elevation in the above parameters in the females of treated group than the females of the control group.

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Article
Occurrence of Escherichia coli in Patients with Urinary Tract Infections in Najaf City
تواجد الايشريكيه القولونية في المرضى المصابين بالتهابات المجاري البولية في مدينة النجف

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Abstract

Abstract: The study was designed to determine E.coli as etiological organism for UTI in some patients & its antibiotics sensitivity pattern. The urine samples suspected with bacteriuria were collected from 130 subjects. A total of 19 isolates from E.coli were diagnosed. Susceptibility testing for 13 antimicrobials was done. The results were analyzed statistically& showed that infection rate in females was higher than males (significant ). Also infection rate in aged groups were significantly higher in 20-29 years ,& 30-39 years .Infection rate in urban individuals had complete ratio in contrast of rural individuals . Our data also revealed that infection rate in housewife women was the highest( significant ),but it was the less in other jobs. The results of sensitivity test appeared that most of used antibiotics were unaffected on E.coli isolates with exception of amikacin ,meropenem ,& ciprofloxacin respectively. Since, cefoxitin was unaffected on all E.coli isolates absolutely.

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Article
Effect of Toxoplasmosis on some biochemical parameters in aborted Women in Al-Najaf Al-Ashraf Governorate
تأثيرات الإصابة بداء المقوسات على بعض المعايير الكيموحيوية في النساء المجهضات في محافظة النجف الأشرف

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Abstract

Abstract: The present study was conducted to evaluate the effects of infection of women with toxoplasmosis on some serum biochemical parameters. 180 samples of blood were collected from aborted women . The percentage of toxoplasma infection depending Enzyme- Linked Immunosorbent Assay (ELISA) to the antibodies IgG and IgM were 42.77%. The results of biochemical analysis showed that infection with toxoplasma caused significant elevation of Nitric Oxide, Malondialdehyde, copper, alanine transaminase and aspartate transaminase enzymes. Aswell as the concentrations of Gluthanione, calcium and zinc were reduced . Where as the activity of alkaline phosphatase did not show any significant difference between aborted women and control group.

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Article
Histopathological observations about the effect of pure nicotine in different doses on ovaries of albino rats
ملاحظات نسيجية – مرضية حول تأثير النيكوتين النقي بجرع مختلفة على مبايض الجرذ الأمهق

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Abstract: The aim of present study was detected the effect of pure nicotine on the histological structure of rat ovaries cortex . In this study was used (24 ) good healthy adult female rats were divided into three groups each group includes (8) animals : The 1st. experimental group was injected (4) mg of pure nicotine subcutaneously ( s/c ) twice daily .The 2 nd. experimental group was injected ( 8 ) mg of pure nicotine ( s/c ) twice daily , while the 3 rd. control group was injected physiological normal saline (0.9 NaCl 2). The experimental period was ( 8 ) weeks . The histopathological observations in the 1st. experimental group was appeared as follow, degenerative changes and disarrangement in the follicular cells & distribution of granulose cells in the follicular fluid .In the 2 nd. experimental group the histopathological changes was include , failure in development of the primary follicles into growing follicles & spread of granulose cells in the antrum within the follicular fluid , as well as vasculization of the follicular fluid & fibrosis was happened in some growing follicles , also the number of atretic follicles was found in the cortex of the ovary .

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Article
Evaluation of inhibitory activity of extracts of Apium gravelens, Coriandrum sativum and Cuminum cyminum against number of pathogenic bacteria
تقييم الفاعلية التثبيطية لخلاصات الكرفس والكزبرة والكمون ضد نمو عدد من الجراثيم المرضية

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Abstract

Abstract: Natural resources especially plants are useful bactericides for the control of bacterial infection .To evaluate the antibacterial potential of three types of plants, essential oils were extracted from the seeds of Apium gravelens, Coriandrum sativum and Cuminum cyminum and assayed in vitro for antibacterial activity aganist most prevalent pathogens Staphylococcus aureus, Salmonella spp.,Escherishia coli and Pseudomonas aeruginosa at 37 º C and 25 ºC.The antimicrobial effect was assessed using agar diffusion method by applying ethanolic solutions of extracts using it in two different temperatures 37 ºC and 25 ºC. The result shows that the extract of Apium gravelens, Coriandrum sativum and Cuminum cyminum alcoholic extract at 25 ºC exhibited an inhibition zone on S. aureus in the concentration 200 mg/ml and had a exhibited significantly (p < 0.05) greater than that produced by gentamicine,also the extract of Apium gravelens at 25 ºC in the concentration 100 mg/ml had a significant antibacterial activity on S. aureus, and the extract of Apium gravelens alcoholic extract at 25 ºC and 37 ºC pronounced antibacterial activity against Pseudomonas aeruginosa in the concentration 100 and 200 mg/ml respectively moreover the inhibition produced by gentamicine. While the antibacterial effect Apium gravelens and Coriandrum sativum at 37 ºC on S. aureus in the concentration 200 mg/ml show very similar effect with gentamicine. The antibacterial activity against Salmonella spp.and E. coli were moderate in action It was found that the investigated extracts of Apium gravelens , Coriandrum sativum and Cuminum cyminum were exhibited a considerable inhibitory effect against S. aureus and extracts of Apium gravelens and Coriandrum sativum against P. aerogene. The significant antibacterial activity appears promising.

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Article
Role of nature and number of parturition in the incidence of uterine prolapse in dairy cows in north Iraq
دور طبيعة الولادة وعدد مراتها في حدوث تدلي الرحم في أبقار الحليب لمنطقة بلد شمالي العراق

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Abstract

Abstract: This study was conducted in the specialized vet. clinic in reproductive diseases and artificial insemination, in Balad region in Saladin province in the northern Iraq. This study included72 cows have uterine prolapse, the number of multipara cows that have uterine prolapse was 49( 68.06%). While the primipara cows with uterine prolapse were23(31.94%), with significant difference between two groups at (<0.01). This study showed that the difficult male parturitions in multipara cows was 23(71.88%)and the normal male parturitions in this group was 9(28.12%). These results were significantly difference between two groups at( p<0.01). The total percentage of uterine prolapse that accompanied the male parturitions in multi para cows was 65.31%. While the number of difficult female parturitions was 12(70.59%) in this group of cows, and the number of normal female parturitions was 5(29.41%). With significant difference at (P<0.01).and the total percentage of uterine prolapse associated with female parturition in the same cows was 34.69%. the study indicated that the number of difficult male parturitions in primipara cows was 9(69.23%), and the number of normal male parturitions was 4(30.77%) in this group of cows , with significant difference between two groups at(P<0.01).and the total percentage of uterine prolapse accompanied male parturitions in primipara was 56.53% .While the number of difficult female parturitions in primipara cows was 7(70%), and the number of normal female parturitions was 3(30%). These results were significantly difference at (P<0.01). with total percentage of uterine prolapse related to female parturitions in this group was 43.47%. Conclusion: it is concluded from this study that the number of parturitions increases the probability of incidence of uterine prolapse, hence the uterine prolapse is more commonly in multipara cows, and the dystocia for both sexes of fetus play an important role in its incidence.

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Article
The effect of cinnamonum aqueous extract on the experimental infection with S.typhimurium & productive parameters &some biochemical blood parameters in broilers
تأثير المستخلص المائي لنبات الدارسين على الاصابة التجريبية بجرثومة S.typhimurium وبعض المعايير الانتاجية وبعض صفات الدم الكيميوحيوية في دجاج اللحم

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Abstract: The study was conducted to detect the inhibitory effect of the cinnamon aqueous extract on the Salmonella typhymurium infection of broiler chicks. A total of 60 Hubbard flex broiler chicks one day old have been raised for 35 days all chicks infected with S.typhimurium(3X104 )cfuml orally at third day of age , randomly divided in to two groups first group is the treatment group(T) received the aqueous extract while the second group conceders as control (C). The results showed significant increasing(p<0.05)for group T in weight gain and feed consumption as compared with control ,also there is an improvement in feed conversion ratio & decreasing in mortality rate but its not significant, the total cholesterol & glucose level significantly decreases while total protein & globulin significantly increased,but no significant differences between tow groups in the concentration of albumin, calcium and phosphorus ,the results showed that the bacterial count decreased significantly at 10,20 days of age for the cecal content count and cloacal swabs samples and decreased numerically at day 20 of age for the cloacal swabs samples, we can conclude that cinnamonum aqueous extract have positive effects of some of the productivity and biochemical parameters and its negative effect on the S. typhimurium.

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Article
Investigation of some diseases of carp at Al-Shamiya city/Iraq
التحري عن بعض الأمراض في اسماك الكارب في مدينة الشامية/العراق

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Abstract: The present study was conducted during (March,April,May,June ,July and August-2009) months to investigate some diseases infected the carp fish at Al shamiya city/Diwaniya provence,715 samples of common carp ,grass carp and silver carp were examined from local market and fish farms . The diagnosis based on case history, clinical& necropsy findings , bacterial culture and wet mount . Recorded results shows, there are 46(6.43%) cases of acute and chronic form of heamorrhagic septicemia , 57(7.97%)caeses of bacterial enteritis, 76 (10.63%)of anchor worms( Lernaea),4 (0.56%) of lice infestation, 3 (0.4%) of cestodes and 65 (9%) cases of Diplostomum spp., the total infection are 251(35.1%) from 715 investigated samples. The study showed there are diversity forms of diseases among carp spp. in which the percentage of parasitic infections is the most abundant form of diseases(20.7%) followed by diseases caused by bacterial infections (14.4%) .

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Article
Effect of pyocyanine extracted from P. aeroginosa on other pathogenic bacteria in vitro
تأثير البايوسيانين المستخلص من P. aeroginosa على بعض الجراثيم المرضيه الاخرى بالوسط الزرعي

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Abstract: Clinical isolates of Pseudomonas aeroginosa was tested for their inhibitory effect on growth of other pathogenic bacteria ,Pyocyanine(PCN) is the major metabolites of this bacteria ,the pigmented strain were found to have stronger antimicrobial activity on Gram's +ve and Gram's–ve bacteria than unpigmented one .Our results revealed the PCN have strongly active against Staph. aureus & E.coli ,but weakly effect of Klebs. & Proteus , other strains of P.aeroginosa are not affected by PCN .

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Article
Diagnostic study of coccidiossis in sheep of AL-Kufa
دراسة تشخيصية لداء الكوكسيديا في الأغنام في قضاء الكوفة

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Abstract: The study were conducted on the percentage of infection of coccidiosis in 175 head of sheep in fefferents area of kuff city and evaluate the effects of age ,sex, and , season during the period from the beginning of (December 2010 to end of June 2011). Samples were collected as 94 samples of meal and 81 samples female. Sifnificant difference were recorded in the percentage infection among the different areas . the highest percent in abbassia and alhurria (85% and 82% ) while the lowest in city center and albarakia ( 76.3% and 73% )the results revealed that the overall percentage of infecting was 78.71% and higher prevalence rates were recorded in sheep less than 7 mouths of age 84.2% the result show differed significantly among different months , the highest percentage was on January and February ( 85.5% and 89.20% ) respectively.

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Article
Potentiate the anticancer effect of Methotrexate by Zingiber officinale Roscoe extract
زيادة تأثير عقار الميثوتريكسيت المضاد للسرطان بمستخلص الزنجبيل

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Abstract: This project considered an explorer study for the effect of crude extracts of Zingiber officinale on two cell lines, one was cancer cell lines, and the other was normal cell lines as well as study its potentiating effect on anti-tumor activity of methotrexate. The aqueous extract was prepared from dried rhizome of Z. officinale yields of extraction was 10%. Study the cytotoxic activity of the prepared extract on cancer cell lines mouse breast 4TI and normal cell line (human embryo kidney HEK293 in ten concentrations of two fold dilution begin with 4000 μg/ml until 7.81 μg/ml for extract and starting with 500 μg/ml until 0.976 μg/ml for methotrxate, at 48 hours of exposure. After the end of exposure of each cell lines to extract, the microtitration plates were washed and treated by crystal violet stain and the optical density of the plate wells were read by the ELISA reader at wave length of 495 nm used as parameter for the viability of cell line. The results showed that the aqueous extract exhibited significant concentration-dependent, specific inhibitory effects on 4TI malignant cell line in comparison with control and the cytotoxic effect increased according to the concentration. The extracts produced little cytotoxic effects on normal HEK293 cell line, when compared to mthotrexate which produced inhibitory effects on normal HEK293 cell line. In this study was showed that the cytotoxic effect of Methotrexat on 4TI cell line enhanced when given with Z. officinale extract together.


Article
Study the effect of coloration diet on Broiler Performance
دراسة تأثير تلوين العلف على الأداء الإنتاجي لفروج اللحـم

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Abstract: The Effect of coloration feed on body weight & feed intake of broiler chickens. The objective of the study was to investigate the colcration of the feed by uses four colours feed (red,yellow,green and blue) on the body weight & feed intake of broiler chickens A total of 50 chicks of one-day fawbro broiler chicks as hatched were divided in to 5 equal group & equal in sex and added one of colour feed in to feed of the one of group and leave tee last group as acontrol group (given anormal feed not coloration). The mean body weight of red feed group was increase (286.3) gm comparative with the mean body weight of control group & (107.9) gm between red feed group and blue food group beside red feed group , (165.8)gm between red feed group and green feed group beside red food group ,(188.1) gm between red feed group and yellow feed group beside red feed group . The mean of feed intake was increas significantly in group of red feeds over control group in all weeks of test & on colour group in more weeks of test.

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Article
An evaluation of neurotoxic effect of metronidazole in rabbits
تقييم السمية العصبية للمترونيدازول في الأرانب

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Abstract: The present study was undertaken to assess the neurotoxic effect of metronidazole in rabbits .Eighteen rabbits were randomly divided into three equal groups ,first group was injected with metronidazole at the therapeutic dose 20 mg/kg.B.W. , second group was injected with metronidazole at double therapeutic dose where as the last group serve as a control .Each animal in all groups was administered intraperitoneally twice daily for (20) days . The evaluations markers have used in this study were monitering of clinical symptoms and determination of histopathological changes in brain and sciatic nerve. The careful observation of clinical signs ,reflexes (patellar ,cross extensor ,and papillary light) and certain responses (pupil size ,nystagmus and a menance response) in first and second group showed a remarkable nervous system dysfunction has improved by histopathological examination of brain and sciatic nerve .there were sever vacuolation in brain ,where the lesion was characterized by spongiform changes ,and degeneration of the nerve bundles in sciatic nerve were detected in rabbits treated with metronidazole at therapeutic dose ,where as there is degeneration and selective loss of parkinjii cells with sever congestion in brain as well as demylination and degeneration in sciatic nerve in rabbits treated with metronidazole at double therapeutic dose .This study revealed that metronidazole has a neurotoxic effect in rabbits (both centrally and peripherally ) with a severity depended on its dose and duration of administration.

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Article
Comparison the effect of Various Cinnamon plant Extracts with Metformin in Blood Glucose level of alloxan-induced diabetic laboratory rats
مقارنة تاثير مستخلصات متنوعة لنبات Cinnamon مع Metformin في مستوى سكر الدم في الجرذان المختبرية المعاملة بالالوكسان المستحدث لمرض السكري فيها

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Abstract : The present study aime to compare the hypoglycemic activity of blood sugar of three types of cinnamon plant extract.Which is methanol,hexane and chloroform extract with metformin drug which is used for type 2 diabetic mellitus in laboratory rats of . The study showed presence of significant p≥0.05 hypoglycemic activity in all cinnamon plant extracts compared with control group.The result also showed highly significant p≥0.05 hypoglycemic activity of hexane extract compared with metformin drug than other extracts after (4,6,9)hr of the treatment.

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Article
Effect of sulphuric acid and formalin on sporulation of Eimeria bovis oocysts
تأثير حامض الكبريتيك والفورمالين على تبوغ أكياس البيضة للـ Eimeria bovis

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Abstract: Eimeria bovis oocysts were isolated and identified after obtained the fecal sample from infected calf. Three different concentrations of sulphuric acid and of formalin were examining to observe their effect on E.bovis oocysts sporulation in addition to 2.5% potassium dichromate as a control. All different concentrations of both chemical solutions (sulphuric acid and formalin) were induced the sporulation of oocyst in addition to the control group (potassium dichromate).

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Article
Detection of biofilm formation among the mastitis isolates of Staphylococci by evaluation of three different screening methods
الكشف عن إنتاج الغشاء الحيوي في المكورات العنقودية المعزولة من التهاب الضرع باستخدام تقييم ثلاث طرق مسح مختلفة

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Abstract: Internalization and biofilm formation is an important step in staphylococcal mastitis pathogenesis . Twenty five staphylococcal isolates of coagulase negative and coagulase positive were collected from clinical mastitis in cows, and biofilm production were studied for these isolate by three different methods. Tube method , tissue culture plate and Congo red agar method. The first two methods were used to study effect of glucose addition as source of polysaccharides that important in biofilm formation. The results showed significant increase in biofilm formation by addition of glucose in staphylococci coagulase negative diagnosed by tissue culture plate method. Congo red agar method results showed increase number of positive isolates by increasing incubation time. The results showed that tissue culture plate method (Spectrophotometric method) was the best method for the diagnosis of biofilm formation in comparison with both tube method and Congo red agar method.

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Article
Actinomycin- Production Assay: A simple Bacteriological Method For Antitumor Activity Screening In Vitro
اختبار إنتاج الاكتينومايسين : طريقة بيولوجية بسيطة لاختبار الفعالية المضادة للأورام خارج الجسم الحي

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Abstract: We present and evaluate the actinomycin-production assay, a bacterial test for detecting DNA-damaging agents as a primary step for detecting the antitumor activity of new synthesized, modified or isolated compounds. It’s a simple test based on the activity of the agents against actinomycin antibiotic production of Streptomyces antibioticus, bacteria whose value is monitored by the inhibition activity of E. coli and S. aureus bacterial growth using two standard anticancer drugs cisplatin and flurouracil. In this assay, production of actinomycin antibiotic is measured as a specific indication of the ability of an agents to directly or indirectly damage DNA and that are of potential interest in carcinogenesis and cancer chemotherapy. Because in vivo tumor test systems using mammalian cell cultures were slow and expensive, we tested a novel cytotoxicity test, simple, sensitive, inexpensive and amenable to large-scale screening using bacteria cultures, as well as, it will offer another practical application in addition to cytotoxicity screening, the assay is used for testing antibacterial of the test compounds.. Key words: Streptomyces antibioticus, Actinomycin, Cytotoxicity test, antitumor activity, antibacterial activity.


Article
The pathogenesis of experimental infection by Staphylococcus aureus in rabbits
أمراضية الإصابة التجريبية للمكورات العنقودية الذهبية Staphylococcus aureus في الأرانب

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Abstract: The present study was carried out on experimental infected rabbit with Staphylococcus aureus to study some aspects of pathogenesis of staphylococcal infection. Twelve rabbit were randomly divided into two equal groups: group1 (treated group) were inoculated subcutaneously with (0.5ml) of bacterial inocula contain (30x1010) CFU and group2 (control). Blood samples were taking to evaluate some of blood parameters include: WBC counts,(LYM., Mon.and GRA.) percentage, Mon.#,GRA.#,RBC counts, HB, PCV, MCV, MCH, MCHC, RDW, PLT counts, MPV, PCT and PDW. Most clinical signs noticed in treated group were: mild fever, depression, decreased appetite, respiratory distress and abscess formation in some cases at site of injection. Also the P.M. lesion included: congestion of internal organs (liver, lung) and signs of pneumonia. Histopathological investigations were carried on liver, lung and bone. The statistical analysis revealed that there were significant increased in WBC count and GRA % (P<0.05) in treated group as compared with control. The MCV, MCH, MCHC and RDW (P<0.05) showed significant differences between groups. The PLT counts and PCT (P<0.05) revealed significant decreased in treated group as compared with control. Other parameters studied did not show significant differences between two groups. Histopathological study revealed that there were: hydropic degeneration, necrosis and occluded of bile sinusoid with bile pigment in the livers of infected animals, in lung: there were edema, hemorrhage and necrosis with thickening of the wall of alveoli and infiltration of inflammatory cells and in bone there were sever necrosis of osteoblast, osteoclast and osteocyte cells in the infected animals.

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Article
Morphological and microscopical comparison features of Bacillus cereus isolates
الصفات الظاهرية والمجهرية لعزلات البابلس سيرس

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Abstract: Fifty seven samples of milk and infant formula were taken to isolate Bacillus cereus. There were 21.55% in milk and 22.5% in infant formula. Polymyxine pyruvate egg yolk mannitol bromothymol blue agar (PEMBA) and blood agar were conducted to grow B. cereus in this study. Colony morphology on PEMBA and Blood agar were used along with gram staining and spore staining methods to compare the morphological and microscopically features with the standard strain. The importance of intensity and severity of this organism in food poisoning due to ingestion of incriminated milk and milk products led to this study. The understanding of the presence and distribution of B. cereus discovered the contamination spots in Baghdad markets and the farms in Baghdad districts to make the authorities able to diagnose these contamination areas in order to control and prevent the infection. The aim of this study is to reveal the availability of B. cereus in milk and infant formula in the local dairy farms and the markets of the districts in Baghdad.

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