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مجلة ذي قار الطبية

ISSN: 19929218
الجامعة: جامعة ذي قار
الكلية: الطب
اللغة: English

This journal is Open Access

حول المجلة

مجلة ذي قار الطبية, تصدر من كلية الطب / جامعة ذي قار. تأسست في سنة 2002, تهتم بالابحاث العلمية والطبية

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معلومات الاتصال

Email: thi_unv_medi@yahoo.com

جدول المحتويات السنة: 2011 المجلد: 5 العدد: 2

Article
THE EXPRESSION OF MISMATCH REPAIR GENES (MLH1 AND MSH2) AND TUMOR SUPPRESSOR GENE (BRCA1 AND P53) IN HUMAN MELANOMA CELL LINES AFTER UVB-IRRADIATION
التعبير الجيني للبروتينات المسؤولة عن تصحيح العطب الوراثي والجينات المثبطة للأورام لخلايا الورم ألقتامي للإنسان بعد التعرض للأشعة فوق البنفسجية

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الخلاصة

ABSTRACT The aim of our study to investigate the susceptibility of melanoma to UVB-irradiation: by examine the expression of hMLh1,hMSH2,BRCA1 and P53 proteins. In our work, we cultured three human melanoma cell lines (Colo38,SK-MEL28 and SK-MEL93, ) in college of medicine university of magna-Gracia-Italy. We first, examined the expression of BRCA1, hMSH2, hMLH1 and p53 proteins by Western Blot analysis in Colo38, SK-MEL93 and SK-MEL28 cell lines. After that the cells were exposed to UV-B (10mJ/cm2)for different point time(1/2,1,3,6,9,12,and 24h). Cells at 0hr were a non-irradiation control. After exposure the expression of MLH1,MSH2,BRCA1 and p53 proteins were assessed by western Blot analysis. We found that the expression of BRCA1,hMSH2 and P53 proteins were increased at 3h after UV-B-irradiation in SK-MEL93 cells line, while, the same amount of the expression proteins in the Colo38 and SK-MEL28 cell lines were obtained . Moreover; our data detected an equal amounts of hMLH1protein in three cell lines, were involved in this study..

الكلمات الدلالية


Article
MEASUREMENT OF SERUM MALONDIALDEHYDE (MDA) LEVELS AS A MARKER OF LIPID PEROXIDATION IN NEONATAL SEPSIS
قياس مستوى المالوندايلدهايد في المصل كعلامة لأكسدة الدهون في الأطفال حديثي الولادة المصابين بالإنتان الدموي

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الخلاصة

ABSTRACT: Neonatal sepsis remains a major problem in neonates admitted to the neonatal intensive care units, with high morbidity and mortality rates despite advances in antimicrobial therapy and supportive cares, especially in developing countries. The lipid peroxidation, as a result of reactive oxygen species (ROS) production, play a significant role in pathogenesis of multiple organ failure and septic shock associated with neonatal sepsis which contribute to high morbidity and mortality of neonatal sepsis. This a prospective study carried out to measure the serum malondialdehyde (MDA) levels as a marker of lipid peroxidation in neonates with sepsis who were admitted to the neonatal care unit at Bint-Al-Huda Maternity and Children Teaching Hospital, Thi-Qar governorate, Iraq from first of April 2010 till the end of August 2011. One-hundred eight septic newborns and sixty matched healthy neonates (thirty were full term and thirty were preterm) as control group were studied. Sepsis was confirmed by clinical manifestations and blood culture. Fifty two (48%) of septic newborns were full term and fifty six (52%) were preterm. The most common microorganism isolated from septic newborns was gram negative bacteria especially Klebsiella pp. The MDA levels were extremely higher in both full term and preterm neonates with documented sepsis than that in their corresponding controls (P-value < 0.001). These results suggest that newborn infants have insufficient defense mechanisms against free radicals. Both enzymatic and non-enzymatic antioxidant mechanisms in neonates with sepsis and usage of antioxidants drugs and vitamins in the management of neonatal sepsis need further evaluation.

الكلمات الدلالية


Article
EXTENT OF RISK FACTORS AMONG PREGNANT WOMEN IN THI-QAR AT 2010
الحد من عوامل الخطر للنساء الحوامل في محافظة ذي قار في عام 2010

المؤلفون: dr. Ali Abd Sa'adoon د. علي عبد سعدون
الصفحات: 18-25
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الخلاصة

ABSTRACT A descriptive cross sectional epidemiological study including 1463 pregnant women in Nasseriyah center of Thi-qar governorate extended from the 1st week of January 2009 till the last week of December 2010was under taken to estimate the extent of high risky pregnancy among antennal care attendants and to relate risk factors with selected socio-demographic characters. The study covered pregnant women attending 6 primary health care centers randomly selected from a list containing all the health centers in the city at. A systematic sample of record- based was used selecting every 5th recorded women with a random starting point. Anaemia, abortion, Cesarean section, hypertension, birth interval less than 2 years were the most prevalent risk factors among pregnant women attending health care centers. There was a strong positive association between socio-demographic characters of pregnant women with the risk factors. High prevalence of risk factors in this study than the other comparative studies. More health education, bettering of recording systems, more attention for the at risk pregnancy were the recommendations of the study.

الكلمات الدلالية


Article
PREVALENCE OF SUBCLINICAL HYPOTHYROIDISM IN PATIENTS WITH CHRONIC RENAL DISEASE IN AL-NASSIRYAH CITY
معدلات هبوط افراز الغده الدرقيه غير السريري عند مرضى عجز الكليتين المزمن في مدينة الناصريه.

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الخلاصة

ABSTRACT Background : Subclinical hypothyroidism is highly prevalent in the general populations especially in elderly, however ,the prevalence of subclinical hypothyroidism in persons with chronic kidney disease is not well studied . Design : Cross sectional data from ( 245 ) persons , 110 persons of them considered as a control group and other persons (135 )with chronic kidney disease , all of them were collected from ( 1-9-2010 to 1-7-2011 ) in Al – Hussein teaching hospital ( out patient and inpatient ). All patients and control group were routinely referred for thyroid function tests , renal function test ( in form of plasma creatinine) and estimation of glomerular filtration rate ( GFR ). Multivariable logistic study was used to evaluate the independent association between prevalent subclinical hypothyroidism and estimated GFR . Results : Among ( 245 ) adult participants with serum creatinine , GFR and thyroid function test results , the mean age for both groups were ( 58 ± 4 ) year , 148 male while 97 of those were female. The prevelance of subclinical hypothyroidism increased specially with lower GFR ( in unit of ml / min. / 1.73 m2 ) , occurring in 7.5 % of subject with GFR (60 – 90 ml /min.) while those patients with GFR less than ( 60 ml / min.) the prevalence were increased to 16.5 % ( P < 0.001 ). The control group were 110 persons , only 3 participant with subclinincal hypothyroidism ( 2 % ). A significant difference when we compared patient with chronic kidney disease and control group ( P < 0.001 ).

الكلمات الدلالية


Article
EARLY APPENDECTOMY DURING PREGNANCY
استئصال الزائده الدوديه المبكر عند الحوامل

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الخلاصة

ABSTRACT Background: Appendectomy for presumed acute appendicitis is the most common surgical emergency during pregnancy, acute appendicitis occurs at the same rate in pregnant and non pregnant women, but pregnant women have a higher rate of perforation. Patients &methods: This prospective study done 42 pregnant women between age 20 to 41 years all of them were complained from signs and symptoms of acute appendicitis arrived ER of Al Hussain teaching hospital in AL Nassyria during period 2010 either came directly or referred from gynecologist ,they underwent appendectomy early after diagnosis . Results: Most of patients succeeded pregnancy 38 patients (90.4%) { in spite of 3 patient (7%) have threatened abortion anther 3 patients have preterm uterine contraction but they continue of pregnancy successively},only 4 patient (9.5%) end with abortion.Most of women [21patients (50%)] complained from acute appendicitis during second trimester .Most of patients who did not delay operation till 48 hours can pass pregnancy successfly with some problems , while who delayed more liable to abortion [4 of 10 patient (40%)] . Aim: reduce fetal loss after appendectomy during pregnancy. Conclusion: we advices early operation in pregnancy with out delay, no place for conservative management in acute appendicitis .

الكلمات الدلالية

acute appendicitis --- pregnancy --- appendectomy


Article
EFFECT OF GOOD GLYCEMIC CONTROL ON LIPID PROFILE IN TYPE 2 DIABETES MELLITUS PATIENTS IN AL HUSSEIN TEACHING HOSPITAL

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الخلاصة

ABSTRACT: Background: diabetes markedly increases the incidence of macrovascular complications. For example, the observed 2- to 3-fold greater risk of myocardial infarction with diabetes rises to 8-fold in the presence of hypertension and to nearly 20-fold if both hypertension and dyslipidemia are present; smoking increases these risks even further. As a result, the diagnosis of diabetes mellitus should quickly prompt both an exhaustive search for coexisting cardiovascular risk factors and the initiation of aggressive preventive measures. Objective: The present study was conducted to evaluate the initial benefit of good glycemic control in patients with type 2 Diabetes Mellitus related dyslipidemia . Patients and methods: Fasting blood sugar, random blood sugar and lipid profile among 150 patients with type 2 Diabetes Mellitus who were regularly attending diabetes and internal medicine clinic in An Nasiriyah General hospital were measured and observed for the period of the study . Results: We found that the level of serum cholesterol especially LDL was significantly low in those with good glycemic control in comparison with those with poorly controlled DM . This reduction in serum cholesterol and LDL would be expected to reduce the risk of atherosclerosis and ischemic heart diseases.

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Article
lINTRAOPERATIVE INTRAVITREAL TRIAMCINOLONE ACETONIDE DURING CATARACT SURGERY FOR THE PROPHYLAXIS AND MANAGEMENT OF POSTOPERATIVE DIABETIC MACULAR EDEMA
حقن مادة التريامسينولون داخل الجسم الزجاجي اثناء عملية المياه البيضاء للوقاية و العلاج من وذمة الشائبه الصفراء

المؤلفون: Dr Wajida Saad Bunian د. واجدة سعد بنيان
الصفحات: 45-59
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الخلاصة

ABSRACT Cataract extraction in diabetic patients is commonly indicated, both for visual rehabilitation and for improved visualization of the fundus. Unfortunately the visual prognosis for diabetic patients undergoing cataract surgery is guarded, mainly because of the risk for worsening retinopathy levels and exacerbation of macular edema. The Aim of this study is to evaluate the efficacy of intravitreal Triamcinolone acetonide injected during cataract surgery in the prophylaxis and management of postoperative macular edema following uneventful cataract surgery in diabetics. The study included 2 groups, Group A included 15 patients divided into 2 subgroups 1)-Diabetic patients without any excising macular oedema., 2)-Diabetic patients with pre-existing macular edema. Patients in Group A were subjected to phacoemulsification with Posterior chamber intraocular lens implantation. Subjects and methods: Group B included 15 diabetic patients with or without preoperatively existing macular edema including patients with previous macular laser treatment with visually significant cataract. Patients were subjected to Phacoemulsification with PCIOL implantation and Intravitreal triamcinolone injection (dose of 8 mg in 0.2 ml will be injected slowly through the inferior pars plana). Results: The results of this study showed that phacoemulsification with intravitreal TA in patients with CSME appears to be a safe intervention to avoid the postoperative exacerbation of the edema in patients with dense cataract precluding macular laser treatment. TA may serve as mean to control postoperative inflammation and prevent exacerbation of the macular edema. Postoperative laser treatment may be needed in some cases to augment the effect of intravitreal TA.


Article
THE POSSIBLE ROLE OF SURVIVIN IN TRANSITIONAL CELL CARCINOMA OF BLADDER
دور السيرفايفين SURVIVIN في سرطان الخلايا الانتقالية للمثانة البولية

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الخلاصة

ABSTRACT Background: Bladder cancer is third most common malignant tumors in both men and women in Iraq. Transitional cell carcinoma ( TCC) account for > 90% of bladder cancer. Survivin, family member of inhibitor of apoptosis (IAP) that play important role in both cell division and inhibit of apoptosis, Survivin express in vast majority of human cancer but not in normal adult tissues. Objective: to evaluate the possible role of Survivin in patient of transitional cell carcinoma of bladder. Materials and methods: formalin fixed, paraffin embedded tissue from (45) patients with transitional cell carcinoma of bladder from Al-Kadhimiya Teaching Hospital in Baghdad were included in this study. In addition, (16) apparently normal bladder biopsies were as a control groups. Tissue blocks were sectioned for detection of Survivin by Immunohistochemistry(IHC). Results: Survivin was not expressed in normal bladder urothelium, and it was over expressed in 71.1% (32/45) of TCC patients (p=0.001) . Overexpression of survivin was associated with tumor grade (p=0.029), although there was no significant association (p=731), but there was different in reading between Survivin expression and muscle invasion. Conclusion: Survivin is highly expression in TCC in compared with normal urothelium, and play key roles in survival of neoplastic cells by negative regulating apoptosis, and its correlated with poor prognosis in cancer.

الكلمات الدلالية

Survivin --- TCC --- IHC


Article
ANTIPROTOSCOLICES ACTIVITY OF NONADECOIC ACID ; PHTHALIC ACID, DIFLOROPHENYL UNDECYL STER AND 1,2- BENZENDICARBOXYLIC ACID , BIS (2-ETHYLHEXYL ) ESTER EXTRACTED FROM CLADOPHORA CRISPATA AND HAPALOSIHON AUREUS COMPARED WITH ALBENDAZOLE
الفعالية الضد الرؤيسات الاولية للمركبات الدهنية (Nonadecoic acid و Phthalic acid, diflorophenyl undecyl ster و Benzendicarboxylic acid , bis (2-ethylhexyl ) ester) المعرزولة من الطحلب الاخضر Cladophora crispata والطحلب الاخضر المزرق Hapalosiphon aureus مقارنة بالالبندازول

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الخلاصة

ABSTRACT: The present study has aimed the hydatid disease , which is endemic in Iraq especially in Basrah city . Fatty acids compound (Nonadecoic acid ; Phthalic acid, diflorophenyl undecyl ster and 1,2- Benzendicarboxylic acid , bis (2-ethylhexyl ) ester ) extracted from Cladophora crispata and Hapalosihon have used and compared with albendazole . The results of the present study have found that compounds have activity against the protoscolices of hydatid cyst similar to the activity of albendazole and in low concentration .

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Article
THE EFFECT OF BODO SALTANS BACTERIAL GRAZING IN ENHANCEMENT OF E. COLI O157:H7 POLLUTED TIGRIS RIVER WATER QUALITY
تأثير الرعي الجرثومي للكائن الابتدائي السوطي Bodo saltans في تحسين نوعية مياه نهر دجلة الملوثة بالبكتيريا القولونية من النمط المصلي O157 : H7

المؤلفون: dr. Talib Hassan Ali د. طالب حسن علي
الصفحات: 82-92
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الخلاصة

ABSTRACT We studied the influence of pathogenic bacteria grazing by bactreiovorous nanoflagellates in a bacterial model community under defined experimental conditions to obtain further insights to understand the ability of using these species as biological control approach against pathogenic bacteria polluted fresh water. By another word, to investigate the ability of genera of (Bodo saltans) bacteriovorous nanoflagellates to graze allochthonous pathogenic, diarrhea causing bacteria strain (Escherichia coli O157:H7) in Tigris River. The investigation was done by monitoring and quantifying the removal (clearance rate) of artificially concentrated bacteria from experimental river water ecosystem at eight selected time points. Our result indicated that pathogenic bacteria consuming as food was greatly sustain the studied nanoflagellate life. Bodo saltans grazing rates of different enteric bacteria species combinations (E. coli + Salmonella typhi) or (E. coli + Shigella flexneri) in mixed culture suggested that Bodo saltans flagellate do have differential feeding behavior among different species of Enterobacteriaceae family. Key words: nanoflagellate, bactreiovory, Bodo saltans

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Article
INFLUENCE OF SOME WEAK ACIDS, WEAK BASES AND SALTS AGAINST SOME PATHOGENIC MICROORGANISMS
تأثير بعض الحوامض الضعيفة والقواعد الضعيفة والأملاح تجاه بعض الحياء المجهرية الممرضة

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الخلاصة

ABSTRACT: This study was designed to investigate of antibacterial activity of many chemical compounds includes : 4 % imidazole, 2 % imidazole, 1% imidazole , 2.5% sodium citrate, 5% sodium citrate, 5% sodium acetate,2.5% acetic acid, 5% acetic acid, 5% citric acid, 5% magnesium chloride (MgCl2.6H2O2), 5% magnesium sulphate (MgSo4), 5% sodium carbonate (Na2Co3), 5% potassium dichromate (K2Cr2O7), 5% potassium permanganate (KMnO4) and 5% sodium hydrogen carbonate (NaHCO3 ) have been carried out against (Escherichia coli, Pseudomonas aeruginosa , Klebsiella oxytoca , Proteus mirablis and Serratia liquefaciens ) that diagnosed by API 20 E technique and Staphylococcus aureus , Pathogenic fungal strains (Aspergillus niger, A. flavus, A. fumagatus, Fusarium sp. and Candida albicans ). The results showed 4 % imidazole, 2 % imidazole, 1% imidazole, sodium acetate , 2.5% acetic acid, 5% acetic acid, 5% citric acid , 5% Na2CO3, K2Cr2O7 have different antibacterial activity according to types of bacteria, whereas 4 % imidazole, 2 % imidazole , 2.5% acetic acid, 5% acetic acid and 5% Na2Co3 only have antifungal activity, and showed compounds: 4 % imidazole & K2Cr2O7 have antimicrobial activity more than standard positive control antibiotic.

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Article
ASSESSMENT OF HEALTH QUALITY OF LIFE UPON SCHOOL AGE CHILDREN (6 – 12 ) WITH THALASSEMIA AT THALASSEMIA CENTRE IN IBN- BALADI HOSPITAL
تقييم نوعية حياة المرضى المصابين بالثلاسيميا في عمر المدرسة (6-12) سنة وعلاقتها ببعض المتغيرات في مركز الثلاسيميا في مستشفى ابن بلدي

المؤلفون: Dr.Khetam Mutsher AL-Mosooi د. ختام مطشر الموسوي
الصفحات: 100-108
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الخلاصة

ABSTRACT Objectives: assess the QOL of thalassemia school age children and relation ship with some variables. Methodology: Non- probability (purposive) sample of (40) patient school age children with thalassemia hospitalized for management were selected .The questionnaire format designed for the purpose of the study, two instrument were used include : sample demographic characteristics QOL domain of thalassemia school children . Data were analyzed by using frequencies, percentage. and multi-regression analysis Results: The result of the study indicated that the majority of the sample was the age group (9-11) and (6-8) and 75% of the subject of the study have (1-2) affected brothers . Regarding the age of onset shows that the majority of thalassemia have the disease between the (6-10) month of there life ,57.5% of the patient suffer from thalassemia major and 67.5% had family history. Over half (72.5%) of patient feel tired when he or she gait due to sign and symptom of there disease , majority of the sample 87.5% depended on blood transfusion and 85% of thalassemia patient need to desferal. While the majority of the patient 85% feel bad because can't do what their friend doing and 70% of patient felt comfort from other supported (nurse, doctor) emotional and psychologically in the center and provide what they need. Recommendation: The study recommend further studies should consider more generic measure of psychological and social functioning related QOL. In addition need for Educational program for those patients should be designed to help them live a better life with hemophilia disease , the patients emphasize the importance of how to cope with their problems for a good QOL.

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Article
MATHEMATICAL MODEL BASED ON SPECTROSCOPY MEASUREMENT AS: A NEW TECHNIQUES OF CARBOXY-HEMOGLOBIN DETERMINATION IN BLOOD
النموذج الرياضي المعتمد على القياس الطيفي ( تقنية جديدة لتحديد نسبة الكربون في الهيموكلوبين الدم)

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الخلاصة

ABSTRACT Simple accurate, non distractive and quick method of detreming the concentration of carboxy- hemoglobin (COHb) in blood has been develop from mathematical model. The method uses two chosen wave length by any spectrophotometer and measure the optical density with other parameter derived from the mathematical model.

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Article
MALIGNANT RENAL TUMORS IN IRAQ (CLINICAL & EPIDEMIOLOGICAL STUDY)

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الخلاصة

ABSTRACT Purpose: The purpose of this study is to highlights any changes in the clinical and epidemiological aspects of malignant renal tumors in Iraq in the last ten years. Methods: 86 patients included in this study , 30 patients in Tikrit center and 56 of them in Basra center, All have renal tumors and radical nephrectomy done for them. Results: The highest incidence is in age group 40-60 years old with younger age group in Basra. The commonest presenting symptom is haematuria and cigarette smoking is the major risk factor. Discussion: The male: female ratio is 1.3:1 in Iraq this may give better prognosis. Regarding age of the patients younger age in Basra and that might be attributed to higher exposure to radiation. The smoking increased the incidence of Renal Cell Carcinoma .We have in Iraq higher incidence (40%) of Renal Cell Carcinoma in comparism to European countries and this, unfortunately, may have poorer prognosis. The incidental finding of Renal Tumors (19%) in this study in comparism to 50% in European countries, this percent can affect the prognosis and can be improved by improving the health care system and population health education.We have higher incidence of big tumors (10-15 cm) in comparism with other studies and also may affect the prognosis.

الكلمات الدلالية


Article
EFFECT OF THE STORAGE ON THE AVTIVITIES OF THE ENZYMES CREATINE KINASE AND LACTATE DEHYDROGENASE IN SERUM AND BLOOD
تأثير الخزن على فعاليه انزيم الكرياتنين كاينيز ولاكتيت ديهايروجنيز في الدم ومصل الدم

المؤلفون: Entessar Abdul-Lateef انتصار عبداللطيف
الصفحات: 127-132
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الخلاصة

ABSTRACT: Objective: This study was carried out to investigate the effect of storage on the activities of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH).For this purpose we measured the levels of CK and LDH activities in the blood and serum samples ,stored at 4C° ,during 0 time, and 24 hour. Method: Total of 51 subjects (32 males and 19 females),mean age was 20 years (range:18-22years) were selected for the study. They were non-smoker, apparently ,healthy persons, their weights within normal range, and they had no family history of diseases and no drug had been taken in last week. Blood samples were collected after an average fasting for 12 hours, and the level of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH) were determined immediately (i.e the zero time) and then later after 24 hours .All samples were stored at 4C°. Results: In serum sample and during 24 hours of storage ,at 4C° the levels of the CK and LDH enzymes approximately remained constant. In plasma, analysis was made immediately after blood collection and after 24 hours . ASignificant increased in the levels of CK and LDH enzymes activities . Conclusion: Based on these results, we can conclude that a contact between blood cells and plasma stored at 4C° for an overnight ,my produce changes in the activities of CK and LDH enzymes.

الكلمات الدلالية

Storage --- blood --- serum. Enzymes activities


Article
THE VALUE OF EARLY PREGNANCY SINGLE SERUM PROGESTERONE MEASUREMENT IN RELATION TO THE FIRST TRIMESTER VIABILITY
استخدام قياس قيمة هرمون البروجستيرون في دم الامهات الحوامل في بداية الحمل كمؤشر لحيوية الحمل

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الخلاصة

ABSTRACT The diagnosis of pregnancy require a multi factorial approach using three main diagnostic test, these are: clinical , laboratory & ultrasound examination. There is no single test currently available which immediately differentiate continuing from non continuing intra uterine or tubal pregnancy(1). Aim of the study To assess the use of single serum progesterone measurement in early pregnancy and its relation to the 1st trimester fetal viability. Patients and methods A prospective study conducted in Basrah maternity and child hospital to assess and predict 1st trimester fetal viability using a single serum progesterone measurement for women who attend the hospital either as their 1st antenatal booking or hospitalization for vaginal bleeding or abdominal pain whom were followed for the end of 13th week of gestation and progesterone levels were compared to the fetal viability using ultrasound examination and results were analyzed statistically. Result The study group enrolled 78 pregnancies, 44 pregnancies had continue viable till 13th week of pregnancy and 34 ended with spontaneous abortion. Serum progesterone level was 5.7+10.9 in continuing pregnancy and 6.7+4.8 in spontaneous abortion. The difference progesterone level was highly significant (p value<0.0001). Conclusion Serum progesterone measurement is a reliable biochemical test in establishing the diagnosis of early pregnancy failure and a predictive test for pregnancy continuation.

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