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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2012 المجلد: 11 العدد: 1

Article
VACCINE PRODUCTION AGAINST Trichophyton Verrucosum ISOLATED FROM MAN AND CATTLES
إنتاج لقاح ضد الفطر Trichophyton verrucosumا المعزول من الإنسان والأبقار

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الخلاصة

ABSTRUCT This study consist of two parts, the first part includes isolation and diagnosis of dermatophytes from infected human and animals. Ninety two samples were collected from young and adult males and females patients The percentage of fungal infections from human dermatological cases was 59.78%. Skin scraping and infected hair samples were obtained from 106 infected animals (cattles and calves) with varying ages and sex, It appeared that 44.33% of cases were infected with ringworm. After exact diagnosis of these samples, T. verrucosum was one of dematophytes that could be isolated from human and animal, and since it represent the major cause of ringworm in cattle with increased proportion of transmission to human, the second stage of this study was aimed by production of vaccine against dermatophytes to decrease the number of cases in animals and then in human. The vaccine was prepared by production of soluble antigens from sonication of veruilent strain of fungus and then inoculated in guinea pigs by using special vaccination program, and after administration of challenge dose they showed resistance at 100%. The whole cell extract of T. verrucosum was used in used in separation process by Gel Chromatography technique with using Sephadex G 100 for separation of it’s protein according to molecular weight, so 2 peaks were observed which used in induction of cell mediated immunity in sensitized animals and for identification of humeral immunity by using passive heamagghitination test to evaluate of efficacy. So the 2’nd peak was found to be possess Biological properties and antigenic ability to induce cellular immune response and serological test, to demonstrate humeral immune.

الكلمات الدلالية

Trichophyton verrucosum; Vaccine.


Article
MOLECULAR DIAGNOSIS OF KLEBSIELLA PNEUMONIAE K1 AND K2 SEROTYPES ISOLATED FROM IRAQI CHILDREN INFECTED WITH SEPTICEMIA
لبكتیریا الكلبسیلا الرئویة ( K و 2 K التشخیص الجزیئي للأنماط المصلیة ( 1 المعزولة من الأطفال العراقیین المصابین بتسمم الدم الجرثومي

المؤلفون: Yasameen A. Kareem Norrya A. Ali
الصفحات: 14-23
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الخلاصة

ABSTRACT Different bacteria were isolated and diagnosed from 200 samples infected with septicemia, taken from children attended IBN–ALBALADY hospital. For bacterial diagnosis the Bact/ALERT 3D and VITEK 2 were used. PCR with specific primers was used to detect the serotypes K1 and K2 of K. pneumoniae. Different bacterial genera in addition to K. pneumoniae were isolated such as Staphylococcus aureus, Enterococcus faecium, Salmonella typhi, Enterobacter cloacae, Acinetobacter lwoffii, E. coli. It was found that Staphylococcus aureus was the more dominant among gram positive bacteria (15 isolates) while K. pneumoniae showed the highest isolated number among the gram negative bacteria (17 isolates). Results of K. pneumoniae identification using PCR technique showed that of 17 K. pneumoniae isolates two isolates belong to K1 serotype, and two isolates belong to K2 serotype which gave DNA band of 1282 bp and 535 bp in size representing magA gene and rmpA gene respectively

الكلمات الدلالية

magA --- rmpA --- Serotypes.


Article
STUDY OF THE RELATIONSHIP OF CagA TOXIN AND IT'S PHOSPHORYLATION SITES WITH THE OUTPUT OF DIFFERENT STRAINS OF Helicobacter pylori INFECTIONS
دراسة لعلاقة السم CagA ومواقع فسفرته مع مخرجات الإصابات بسُلالات مختلفة من Helicobacter pylori

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الخلاصة

ABSTRACT Helicobacter pylori causes different digestive system diseases. H.pylori has different virulence factors, among them CagA which is phosphorylated at tyrosine residue located in special motif of five amino acid "EPIYA". CagA toxin may have different number and EPIYS(s). Relationship between the CagA(s) with EPIYA or without and diseases in different countries was used by using the sequences in public databases. The analyses revealed that there is positive correlation between increasing number of EPIYA(s) and different ulcers; the correlation coefficient r reached +0.866 in peptic ulcer. The relation of EPIYA(s) number and cancer(s) showed inverse relationship as well with gastritis and unknown diseases and r values were negative (-0.421,-0.537respectively). It has been concluded that presence of motifs and their phospharylation sites are not the chief reasons for disease induction such as cancer


Article
UTILIZING NOSTOCLINKIAALGA IN REDUCING CONCENTRATIONS OF DYES PRODUCED FROM TEXTILE INDUSTRIES AND DRAINING TO THE AQUATIC ENVIRONMENT
إستعمال الطحلب Nostoc linkia في خفض تراكيز الأصباغ المستخدمة في الصناعات النسيجية والمصرفة للبيئة المائية

المؤلفون: أمل حمزة حمود --- أحمد عیدان --- أنعام نوري علي
الصفحات: 38-50
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الخلاصة

ABSTRACT One genus of algae Nostoclinkiaas dried and livingwas used in reducingthe concentration of direct blue and reactive red dyes and color in textile factories. The proper concentration 1 gm/L was prepared for each dye, however 0.005. 0.01, 0.03g/L were prepared from this concentration and used for 4 days of treatment. The results confirmed that the efficiency of 1g from dried alga in reducing concentrations of dyes increased during period of treatment which recorded removal percent for direct blue at 0.005. 0.01, 0.03g/L concentrations as 83.11%, 73.05%, 63.6% after 4 days of treatment,while the removal percent for the same dye with 0.5 g of alga was57.44%,71.57% ،56.23% respectively. Living filamentous alga was recorded higher removal percent for reactive red at 0.005, 0.01, 0.03g/L concentration after 96 hrfrom treatment 72.88%, 61.63%, 34.75% respectively. The results illustrate increasing in algal biomass with increasing period of treatment and algal biomass in treatment, algal biomass of 1 g after 96 hr from treatment with direct blue dye were recorded 2.682,2.564,2.33g while algal biomass at 0.5 g after the same period of treatment 1.531, 1.395, 1.274grespectively. Also, the living alga recorded increasing in biomass 47.73, 43.73, 34.97mg after 96hr from treatment with reactive red

الكلمات الدلالية

Textile industries --- Biological treatment --- Algae --- Industrial dyes.


Article
IDENTIFICATION OF TRICHOMONAS VAGINALIS USING MOLECULAR METHODS IN IRAQI INFECTED WOMEN
تشخیص طفیلي في النساء العراقیات المصابات به بالطرق الجزیئیة Trichomonas vaginalis

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الخلاصة

ABSTRACT There are different methods for identification and detection of Ttrichomonas vaginalis which causes trichomonasis. In this study Polymerase chain reaction (PCR) for amplification of 18S rRNA gene was used. Three hundred and four vaginal swabs were collected during the period (December 2010- September 2011) from hospitals of north east of Baghdad city from ladies complained of genital tract infections. There were 148 cases suspected to be trichomonasis, among these suspected cases there were 60 cases (40.5%) of real infections with the parasite. These infections distributed on different age groups, and the highest was in the reproductive age group (21-30) years and it was 40%of cases, and the parasite was detected in complicated cases such as inflammation and other symptoms (30%), followed by pregnancy(18.33%). Trichomonasis combined with elevation of vaginal pH and most of cases (31 cases out of 60 cases) detected at pH 6. Trichomonasis combined with elevation of WBCs count/ml (12000-15000 WBCs/ml) blood and pus cells in vaginal discharge، 31 pus cell/field. Using Polymerase chain reaction for amplification of 18S rRNA showed sensitivity at 100% and specificity 100% to amplify segment 312 bp. Positive results with Polymerase chain reaction appeared in samples which were negative in other tests such as direct microscopy، pH, Wiff test, Rapid test based on the immunity

الكلمات الدلالية

Trichomonas vaginalis --- Molecular PCR --- 18S gene.


Article
THE EFFECT OF INHIBITORS ON PURE UREASE ISOLATED FROM LOCAL ISOLATE PROTEUS VULGARIS
Proteus vulgaris تأثیر المثبطات الیوریز المنقى من البكتریا المتقلبات المعزولة محلیاً

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الخلاصة

ABSTRACT Examination the urease activity which purified from Proteus vulgaris in the presence of six inhibitors were thiourea, hydroxy urea, EDTA, ß-Mercaptoethanol, flurofamide and Para - Benzoquinon has been observed that all these compounds had been urease inhibitors, results showed that all of them where competitive inhibitors except p- benzoqinon non-competitive. The mechanism inhibition were studying of all inhibitors compounds, there ß-Mercaptoethanol has regained enzyme effectively with the p - benzoquinon, which indicates the existence of a sulfhydryl group (SH) on-site effective in not affected by the act of each thiourea and hydroxurea and the EDTA, either when using ions nickel, all inhibitors may affected by their work, except ß -mercaptoethanol and p-benzoqinon and not recovery the enzyme activity, which indicates that the binding site inhibitors is effective, and the results of the current study appeared that the inhibitor flurofamide was the most effective inhibitors of the urease.


Article
BIOLOGICAL TREATMENT OF HYDROCARBON COMPOUNDS IN OIL REFINERY WASTE WATER
المعالجة الحیویة للمركبات الهیدروكربونیة في میاه الصرف لمصافي النفط

المؤلفون: Hasanain A. Husein --- Essam F. AL-Jumaily --- Alaa K. AL-Dulaimy
الصفحات: 77-89
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الخلاصة

ABSTRCT Three types of microorganisms are used in this study to show the difference between their ability in consumption of the hydrocarbon residual in the industrial waste water of the Al-Doura refinery. These types are: Protozoa (taken from Al-Doura refinery) and two local isolates: P. aeruginosa and P. fluorescens. These have been already tested boichemically, physiologically and according to API 20E system method. Box-Wilson method is used to find the mathematical relations between the variables (temperature, pH and inoculate amount) with biomass amount during the hydrocarbon residual consumption. The obtained experimental results are fitted to a polynomial function of second order. The experiments are carried out using batch culture for both the three isolates (Protozoa, P. aeruginosa, P. fluorescens) and the mixed isolates (P. aeruginosa+ P. fluorescens). The best incubation period for the three isolates is 72 hours. the optimum operation conditions for mixed isolated are biomass 5.22 g/L, BOD5 15 mg/L, TSS 95 mg/L, TDS 4500 mg/L and S.T 25 m.N/m. These results have show that mixed isolates are the best for consumption of hydrocarbon residuals

الكلمات الدلالية

Bioreactor --- Pseudomonas --- Waste water --- Biotreatment.


Article
PROTECTIVE EFFECT OF FLAVONOIDS EXTRACTED FROM PARSLEY (PETROSELINUM SATIVUM L .) LEAVES ON LIVER FUNCTION IN MALE RATS EXPOSED TO CADMIUM CHLORIDE
التأثير الوقائي للفلافونيدات المستخلصة من أوراق البقدونس على وظيفة الكبد في الجرذان المعرضة لكلوريد الكادميوم

المؤلفون: Aous K. Ahmad --- Khalisa K. Khudiar
الصفحات: 90-108
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الخلاصة

ABSTRACT This study was carried out to investigate the protective and antioxidant effects of the flavonoids extracted from (Petroselinum sativum) parsley leaves on hepatic function in adult male rats exposed to 50 ppb cadmium chloride (CdCl2) in drinking water. Crude flavonoids were extracted from parsley leaves. Further purification of flavonoid was performed by gel permeation column chromatography on Sephadex LH-20. Then it was identified by thin layer chromatography (TLC). The results showed that the yield of crude flavonoids from parsley leaves was approximately 2.68 % of dry leaves. Purification of crude flavonoids on Sephadex LH-20 clarified three peaks activity and the proportions of the purified fractions P1, P2 and P3 were 14.06, 82.43 and 3.5 % respectively. Thin layer chromatography confirmed that P2 was pure apigenin depending on its Rf value which is similar to that detected previously by Harborn(1984). According to available literature, apigenin was purified in this study for the first time in Iraq comprised 0.47% of dry parsley leaves. Thirty adult Albino male rats were randomly and equally divided into three groups (10 rat/group) and were treated daily for 60 days as follows: Group C (control), Group T1: Rats of this group received drinking water containing 50 ppb of CdCl2, Group T2: Rats of this group were given orally 150 mg/kg B.W. of flavonoids (apigenin) extracted from parsley in addition to 50 ppb of CdCl2 in drinking water. Fasting blood samples were collected by cardiac puncture technique at 0, 30 and 60 days of experiment to measure the following parameters: Serum glutathione (GSH) and malondialdehyde(MDA)concentrations, Serum aspartate aminotransferase (AST) and alkaline phosphatase(ALP)activities.The results revealed that animals exposed to 50 ppb of CdCl2 in water for 60 days suffered hepatic damage manifested by a significant elevation in the serum AST and ALP activities, as well as a case of oxidative stress as indicated by the significant elevation in serum MDA and suppression in serum GSH concentrations. Intubation of flavonoids concurrently with CdCl2 caused a significant correction of the above mentioned studied parameters. Moreover, occurrence of liver damage as a result of cadmium exposure characterized by inflammatory cell infiltration as well as hypertrophy and hyperplasia of hepatic cell has been emphasized. While oral intubation of flavonoids caused regression of the lesion. In conclusion the results of this study have confirmed the deleterious effect of Cd on liver functions and documented the hepatoprotective and antioxidant effects of flavonoids extracted from parsley

الكلمات الدلالية

Oxidative stress --- CdCl2 --- Apigenin --- Liver functions.


Article
PHOTODYNAMIC THERAPY OF SUBCUTANEOUS MURINE MAMMARY ADENOCARCINOMA
العلاج الحركي الضوئي بالحقن داخل ورم سرطان الغدد

المؤلفون: Ahmed M. H. Al-Shammari2 --- Ahmed S. K. Al-Khafaji1
الصفحات: 109-120
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الخلاصة

ABSTRACT Photodynamic therapy (PDT) of cancer is a treatment based on the accumulation of a porphyrin-related photosensitizer in tumor cells, and their subsequent destruction based on exposure to light source of specific wave length. Hematoporphyrin derivative has been shown a selective localization in malignant tissues and causing their destruction by generated singlet oxygen when it's activated by appropriate wavelength (λ) of irradiation. Singlet oxygen species are produced and then caused membranes' and organelles' damage leading to cell death and tumor ablation. In this study, Female mice transplanted with AM3 (mouse mammary adenocarcinoma transplantable tumor line) randomly divided into four groups of 10 mice each, the mice of first group were intratumorally injected with 30 mg HPD/kg of body weight and exposed to 10 minutes of irradiation from 20mW He-Ne laser (λ= 632.8 nm). The mice of second group were intratumorally injected with 30 mg HPD/kg of body weight without irradiation. Third group mice were received 10 minutes exposure time of He-Ne laser irradiation without HPD injection, while the fourth group was leaved as a control. Photodynamic therapy, which includes HPD and irradiation, has had the most powerful effect on the tumor growth, while the other groups have not showed any significant response. With more investigations PDT can be promising anti-breast cancer arsenal


Article
DETERMINATION OF TESTOSTERONE, INFLAMMATORY PROTEINS AND SEVERAL BIOCHEMICAL PARAMETERS IN SERA OF PATIENTS WITH ATHEROSCLEROSIS
تحديد مستوى التيستيرون والبروتينات الإلتهابية وبعض الدلائل الكيميائية في مصل مرضى تصلب الشرايين

المؤلفون: Noora S. Mubder2 --- Asmaa M. Salih1 Fayhaa M. Kaleel2
الصفحات: 121-132
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الخلاصة

ABSTRACT The present study aims to investigate the concentration of testosterone with some inflammatory proteins ( Ceruloplasmin and Albumin ) and Lipid profile in order to evaluate their role in Atherosclerosis and the relationship between them. The study included 33 male patients with age range (40-75) years, 25 healthy males to be used as a control group with age range (39-63) years. Testosterone showed significant decreasing level compared to control group, while Ceruloplasmin and total serum protein showed significant increasing levels with non- significant decreasing level of albumin in sera of the same patients compared to control. At the same time, Cholesterol and Triglyceride showed significant increasing levels while decreasing level of high density lipoprotein in sera of patients group compared to control group was observed. In conclusion, the present results shows negative correlation between Testosterone with inflammatory proteins and between Testosterone with other Biochemical markers which may show a relationship between low level of Testosterone, inflammatory proteins and other markers


Article
CLINICAL IMPORTANCE OF INTERLEUKIN-8 CONCENTRATION IN IRAQI BREAST CANCER PATIENTS
الأهمية السريرية لتركيز الأنترليوكين الثامن عند مريضات سرطان الثدي العراقيات

المؤلفون: Siaf ALdeen M. Mohsin --- Siaf ALdeen M. Mohsin --- Amina N. Al-Thwani
الصفحات: 133-140
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الخلاصة

ABSTRACT The aim of this study is to estimate the role of interleukin-8 in breast cancer Iraqi women and to assess the correlation between the serum levels of IL-8 with the progression of disease stage. Fifty patients with proved diagnosis of breast cancer by clinical examinations presented to the Al alwyah hospital for gynecological diseases and obstetrics (Breast cancer early detection clinic) in Baghdad. All were females and didn’t have received any chemo/or radiological treatment .Also, 20 samples of apparently healthy women were involved as a control. The blood samples (5ml) were drown from all of the studied cases in order to be used for measuring their serum level of IL-8 by using Enzyme–Linked Immunosorbent Assay (ELISA). The results investigated that Serum IL-8 level were increased with high significant in breast cancer patients and showed significant correlation in early disease stages compared to control group with the highest level in stage I group of patients. As a conclusion, serum level of IL-8 is highly correlated with breast cancer and might have detecting and diagnostic usefulness

الكلمات الدلالية

Interleukin-8 --- Breast cancer --- Iraqi women. Stages of breast cancer

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