Table of content

Jornal of Biotechnology Research Center

مجلة مركز بحوث التقنيات الاحيائية

ISSN: 18151140
Publisher: Al-Nahrain University
Faculty: Presidency of the university or centers
Language: Arabic and English

This journal is Open Access

About

The Biotechnology Research Center (BRC) in a Al-Nahrain University issued in 2007 the first edition of a tightly seasonal scientific journal named as the journal Biotechnology Research Center (JBRC) which got authorization in 2005 and held an impact number ISSN: 1815-1140.The journal accepts scientific researches in Arabic and English.
JBRC’s main interest is Biotechnology researches in the Medical, Molecular, Agriculture and environmental fields which have an important impact on the public and private sectors in Iraq.
JBRC’s structure consists of editing committee (headed by the manger of the BRC and the editor in chief), consulting committee (contains a well- known iraqi scientists in biotechnology) and editing secretary.
All researches are submitted to the JBRC’s regulations which is mainly is that they must be according to the journal directions and instructions , then the researches will be evaluated by three well-known scientists in the field and after that reviewing occurred by the editing committee to ensure and verify all JBRC’s instructions and regulations are taken into consideration .
A special edition of JBCR is issued to cover all researches that presented to the BRC’s scientific conferences which subjected to all regulations and instructions of publishing in JBCR.

Loading...
Contact info

brcn2012@yahoo.comيتم الاتصال عبر البريد الالكتروني لمجلة مركز بحوث التقنيات الاحيائية


0096407707766148او للاتصال تلفونيا
بسكرتارية التحرير
م.م. سعاد محمد مجيدب
محمد منير حسين

Table of content: 2013 volume:7 issue:1

Article
Optimum conditions for isoamylase production by Pseudomonas sp.
تعيين الظروف المثلى لإنتاج إنزيم ايسواميليز بوساطة بكتريا Pseudomonas sp.

Loading...
Loading...
Abstract

Different nutritional and cultural factors were studied to determine the optimum conditions for isoamylase production by Pseudomonas sp. in a batch culture of the production medium. These factors include carbon, nitrogen and phosphate sources and their concentrations, temperature and pH. Results showed that the optimum conditions for isoamylase production by Pseudomonas sp. were achieved when the production medium was supplemented with maltose 1%, peptone 0.4%, and K2HPO4 0.4% as a carbon, nitrogen, and phosphate sources respectively, at initial medium pH 6, and incubation at 28°C for 24 hours. Under these conditions isoamylase productivity reaches the maximum, at which enzyme specific activity was 0.85 U/mg proteins.

Keywords


Article
Effect crude aquatic extract of leaves Ocimum basilicum on caner and normal cell lines in vitro
تاثير المستخلص المائي الخام لاوراق الريحان Ocimum basilicum على الخطوط الخلوية السرطانية والطبيعية خارج الجسم الحي

Loading...
Loading...
Abstract

Leaves of Ocimum basilicum were extracted with distilled water to prepare aquatic crude extract, five concentration from this extract (1000, 500, 250, 125, 62.5)µg/ml were used to study the effect of extract on cancer cell lines(Larynex carcinoma hep-2, cervix carcinoma Hela and mammary gland adenocarcinoma AMN-3) and the time exposure 24 and 48 hours as well as the effect of aquous extract on normal cell line for embryonic mice fibroblast(MEF) was studied in vitro at 48hrs. exposure. The result showed that aqueous crude extract of Ocimum basilicum leaves has different effects on cancer cell lines with significante p<0.05 the high concentration 1000 µg/ml has more inhibitory effect on cancer cell line Hep-2 compared with low concentration at 24and 48 hrs.while the Hela cancer cell line has hormetic effect which recognized by contrast in low concentration inhibitory effect as compared with high concentration at 24 and 48 hr.that low ones inhibit cell proliferation while in high ones cell proliferation continue, but AMN-3 cancer cell line more affected by low concentrations from high concentrations. Normal cell line show no significant effect for all concentrations used of aquatic crude extract of Ocimum basilicum leaf except 62.5 µg/ml with high cell inhibition 16%.


Article
Antitumor and Immunomodulatory Activities of Peganum harmala Extracts
فعاليات مستخلصات نبات الحرمل المضادة للاورام وكمعدلات مناعية

Loading...
Loading...
Abstract

This study was designed to search the antitumor potential of Peganum harmala methanolic extracts and to evaluate their role in retardation the immunological side effects pre-and post-treatment which were carried out on 30 Albino male mice and included total and absolute count of leucocytes, micronucleus formation and phagocytic index of peritoneal cells. Our study showed that P. harmala derivatives have considerable antitumor activity with high efficacy in immobilization of the immunological side effects evoked following treatment with antitumor drug. In conclusion, P. harmala possess antitumor activity increases with concentrations increasing. Pre-treatment with these extracts exert higher retardation to the side effects than post-treatment.

Keywords


Article
Effect of magnetic field of both north and south pole on the growth and viability of the local isolate Aspergillus flavus sh1 and its capability for the production of aflatoxin
تأثيرالمجال المغناطيسي للقطبين الشمالي والجنوبي على نمو وحيوية العزلة المحلية للفطر وانتاج سم الافلاتوكسين Aspergillus flavus sh1

Loading...
Loading...
Abstract

This study included the investigation of the effect of magnetic field of both north and south pole on the growth and viability of the local isolate of Aspergillus flavus, and its capability for the production of aflatoxin.The results showed that the north pole effect of the growth and viability of the tested isolate and it's capability for aflatoxin production. The colonies diameter were (50,60,70 )mm to the (north pole, south pole, north and south pole) treatments and the control was (60)mm. The numbers of spores to the same treatments were (12×107 ,50×107,31×107) spore/100 ml while the control was (30×107) spore/100ml.The dried weight of the same treatments were (0.11,0.36,0.25)gm while the control was (0.23)gm.The south pole show an efficiency in staining Aspergillus flavus sh1 comparing with the north pole. And so the North Pole was reduced the production of aflatoxin, while the South Pole increased the aflatoxin production.

Keywords


Article
Screening of genetically modified Corn Zea mays in Iraqi market
التحري عن محصول الذُرة Zea mays المحورة وراثياً في الاسواق العراقية

Loading...
Loading...
Abstract

Detection of the genetically modified crops could be done by screening certain markers usually used in modification. In this study polymerase chain reaction (PCR) technology was used to investigate the presence of the promoter P35s and nos terminator in the genetically modified corn Zea mays. 72 samples of the maize crop collected from inside Iraqi market from various sources, including imported crops and other local strains used for agriculture or for the production of animal feed. DNA extracted from the corn seeds by two methods, the efficiency of extraction was compared between the two procedures, the purity of DNA samples extracted ranged between 1.4- 1.8 of the samples studied, while the ranged values for concentrations ranged from (500-2400) ng /µl, specificity of the DNA extracted was confirmed using Zea mays specific gene responsible for production of Zein protein, a storage protein. Results shows that all the samples were positive for this gene, results of the investigation of sequence responsible for regulating gene expression for promoter P35s and T-nos terminator, should that 10 samples 13.9% of the total 72 samples studied are genetically modified and gave positive results for the amplification of PCR using primers specialized for each of the P35s and T-nos. The results indicated that (9 out of 47) represent 19.14% of the samples studied imported for the government institutions were genetically modified. Multiplex PCR technique used for the detection of two types of the targets at the same reaction to reduce the time and efforts. Multiplex PCR successfully applied for two combinations of either zein and P35s or zein and nos.

Keywords


Article
Detection of anti-Myalin and anti-axonal auto-antibodies in Iraqi patients with multiple sclerosis
دراسة الاجسام المضادة الذاتية للمايلين والمحاور العصبية لمرضى تصلب الاعصاب المتعدد في العراق

Loading...
Loading...
Abstract

Multiple Sclerosis (MS) is characterized by demyelination of different parts of the central nervous system (CNS) leaving scars (sclerosis) which leaves the neuron and the axis highly attenuated. It is one of the most important inflammatory diseases of the CNS that causes deficits with the progression of neurological disease. This study included 65 MS patients (37 relapsing-remitting (RR), 21 secondary-progressive (SP), 7 primary-progressive (PP) and 50 apparently healthy individuals controls. The results showed low level of auto-antibodies to myelin associated glycoprotein (anti-MAG) in MS patients, while anti-axon antibodies had high level. This means that the production of these auto-antibodies in different stages of the disease increase its effect in the diagnosis and the possibility that it had some role in the progress and development of MS, so the use of anti axonal antibodies test in the diagnosis is an important marker.


Article
Biofilm production as a virulence factor in Uropathogenic bacteria and yeasts
انتاج الغشاء الحيوي كعامل ضراوة في البكتيريا والخمائر الممرضه للجهاز البولي

Loading...
Loading...
Abstract

This study includes isolation and identification of different uropathogenes (bacteria- yeasts) collected from catheterized patients suffering from complicated urinary tract infections. Three hundred and fifty urine samples obtained by swabs from catheterized patients were identified for the presence of uropathogenes (bacteria and yeast). 221(63.13%) samples were obtained from females and 118 (33.71%) samples from male, 339(96.85%) sample were identified by culturing as a positive result, while 11(3.14%) sample were negative result. The 339 positive isolates include 303 (89.38%) bacterial isolates and 36 (10.61%) yeast isolates. Results of biochemical tests and Api 20 system for bacterial and yeast isolates reveals E.coli, Proteus spp, Klebseilla spp, Pseudomonas spp and Candida spp represented the main causative uropathogen infect urinary system and causing a complicated type of infection. The determination of bacterial and yeasts ability to form biofilm was carried out using test tube method , 306 isolates which represented (90.26%) were capable to form biofilm with differ in the thickness of formed layer. Pseudomonas spp formed the thicker biofilm followed by E.coli, Candida spp, Proteus spp, and Klebseilla spp.

Keywords


Article
Role of Staphylococcus aureus cell wall protein A in the pathogenesis of the bacteria
دور بروتين A المستخلص من جدران خلايا بكتريا Staphylococcus aureusفي امراضيتها

Loading...
Loading...
Abstract

This study dealt with 20 diagnosed isolates of Staphylococcus aureus. The haemagglutination test was used to investigate the presence of protein (A) in all S.aureus isolates. One isolate has been chosen as an optimal isolate to accomplish this study, because of its high content of protein (A). Crude protein (A) was extracted by two steps. The first is "Suspending in sodium azide"; the second was precipitation by ammonium sulphate. In order to get a purified protein (A) extract from crude protein A, purification was accomplished by two steps: Ion – exchange chromatography by DEAE – cellulose column was used in the first step, while Gel – filtration chromatography by Sepharose CL – 6B column was used in the second step. Double immunodiffusion in gel was used to investigate the preservation of the crude and purified protein (A) with its full function. Bradford method was used to estimate protein (A) extracts concentration (Crude, Partial purified, Purified). White mice (Balb C) were used to investigate the pathogenic effects of purified protein (A) .The injected mice with purified protein (A) showed general weakness which was very clear after the sixth injection . There were no phenotypic dermal change in the injection area and no dead mice as well. After killing and dissecting of all the mice, the organs of heart, spleen, liver, kidney, intestine, stomach and lung were eradicated. There were a great enlargement in spleen and a little in liver in all injected mice in comparison with the control mice. No phenotypic changes appeared in heart, intestine and stomach, but black spots were shown on the outer surface of kidney and liver tissue which were presented in all the injected mice. The most important results in this study are the great size of pathogenic effects in lung. The tissue seemed faint black with which was obvious in seven mice. The lung of the three others (some parts) showed during anatomy covered by blood. The histological check for heart, intestine and stomach showed no pathogenic effects resulted from protein (A) injection. In the spleen, there was a huge expanding in the white pulp region, with blood congestion while the spreading of megacaryocytes has increased. The liver showed mild degenerative change in liver cells with monocytes infiltration especially in the entrance region which appeared expanded. Also there was an increase in kupffer cells in all liver tissue. Kidney showed increase in mesengial cells which are considered the forming units of glomeruli. The histological check of lung included bloody congestions, oedema, inflammatory cells infiltration and alveolar spaces expanding forming ''Emphysema '' The lung was the most effected organ that showed tissue injury among other all organs.

Keywords


Article
Prevalence of hepatitis G virus (HGV) among hepatitis-B patients and others suspected for PT-hepatitis in Baghdad
دراسة انتشار فيروسHGV بين مرضى HBV واخرين مشكوك اصابتهم بالتهاب الكبد الفيروسي المنتقل عن طريق الدم في مدينة بغداد

Loading...
Loading...
Abstract

The aim of this research is to evaluate the prevalence of HGV among three different categories, group1, composed of hepatitis –B patients (HB- patients), group 2, included people having hepatitis-like symptoms with sera negative for HBV and HCV markers, referred to as suspected for hepatitis (SUS-patients) while the third group is the control group. Serum samples were assayed for anti-HGV antibodies using ELISA technique-indirect method. Results revealed that HGV coinfection detected in only few number of HB-P 9.8%, 23.5% of HB-P having anti-HGV antibodies with titer lower than the cut-off value (COV) which are said to be in the shadow zone and the other 66.6% of the group are reported as negative cases, reporting a highly significant difference P˂0.001. Among the second group (SUS- patients), HGV was detected in only 4.8%, 58.5% detected as shadow cases while the other 36.5% were found to be negative for HGV.When both HB-P and SUS-P are gathered in one group a low percentage 7.6% of HGV infection was recorded, 39.1% of HB-/SUS-patients were in the shadow zone while the majority of this group 53.2% were detected as negative cases. In conclusion HGV play only a minor role as a confection agent with HBV and as a responsible agent among non-A-E hepatitis cases. Remarkable high percentages of shadow cases are reported in the three groups especially among non-A-E hepatitis patients.

Keywords


Article
Efficiency of some bacteria to degrade direct Blue Dye in water
كفاءة استخدام بعض انواع البكتيريا لتحطيم صبغة Direct Blue في المياه

Loading...
Loading...
Abstract

Bacteria Bacillus sp., Lampropedia hyaline and Azotobacter chroococcum were used to degrade Direct Blue dye which is use in textile factories due of its toxic effect from water in concentrations (0.005, 0.01, 0.03) gmL in laboratory by measuring Absorbance (Abs), Transmition T% for 24 hr period treatment with bacterial growth density of (3500 cellml), (17500 cell5ml) and (175000 cell10ml) for each bacteria. Azotobacter and Bacillus showed high ability to reduce dye concentrations of 0.005 and 0.01 gmL by 50-80% and 30-60% in 24 hr treatment. While, Lampropedia showed less ability to reduce all dye concentrations by 2-20%. Bacillus and Azotobacter showed less ability to reduce concentration of 0.03 gmL by 20-50% and 15-40%.

Keywords


Article
Extraction and identification of phenol compounds from Bitter Melon Momordica charantia fruits and their role as antioxidants
استخلاص وتشخيص بعض المركبات الفينولية من ثمار نبات الكيريلا Momordica charantia وفعاليتها المضادة للأكسدة

Loading...
Loading...
Abstract

The antioxidant and free radical scavenging activities of Bitter Melon Momordica charantia exracts including phenolic compounds, ethanolic and aqueous were studied. Phenolic compounds were extracted, purified and identified by High Performance Liquid Chromatography (HPLC) method. The main phenolic constituents, which were present in the fruit extract of bitter melon, were gallic acid, protocatechuic acid, gentisic acid, catechin, chlorogenic acid and epicatechin. The results clearly indicated that phenolic compounds have an effective antioxidant activity by using Ferric Thiocyanate (FTC) method. Phenolic compounds caused 91.25% lipid peroxidation inhibition of linoleic acid emulsion. This activity was greater than ethanolic extract 82.5%, - tocopherol 70% and aqueous extract 49.58%. Also the phenolic compounds revealed obvious activity for H2O2 scavenging 68.8% in comparison with - tocopherol 45.3%, ethanolic extract 52.6% and aqueous extract 36.2%. These results confirmed the important role of phenolic compounds as antioxidants and the most antioxidant activity of bitter melon fruits belong to these compounds.


Article
Biodegradation of organic pollutants in oil industry waste water by Bacillus Bacteria
التحلل الحيوي للملوثات العضوية في مياه مخلفات الصناعات النفطية باستخدام بكتيريا Bacillus

Loading...
Loading...
Abstract

Bacillus apiarus, Bacillus filicolonicus and Bacillus laterosporus were used to degrade organic pollutants from final discharge of Al Dura wastewater treatment plant in Baghdad and reduce their concentrations by measuring Chemical Oxygen Demand (COD), Total Organic Carbon (TOC), Total Nitrogen (TN) and Total Phosphorus (TP) in laboratories conditions of 25o c, pH 5.8-6.8 in batch culture system and 18hr period treatment, the bacteria Bacillus apiarus, Bacillus filicolonicus and Bacillus laterosporus showed ability to reduce COD concentration by(82, 88.5, 94)% and TOC concentration by (73, 82 ,84)% and TN concentration reduced by (86, 91, 81)% in 18 hr period treatment with highly significant differences P<0.01 for all bacterial treatments, the bacteria in the current study showed high ability to reduce TP concentration by 100% with highly significant differences P<0.01 for all bacterial treatments in 18hr period treatment.

Keywords


Article
Histological study for the biological effect of Rosemary Rosmarinus officinalis L. essential oil on liver and kidney tissues
دراسة نسيجية للتأثير البايولوجى لزيت نبات اكليل الجبل على انسجة الكبد والكلى

Loading...
Loading...
Abstract

Many herbs and plant extracts are added to the diet not only for their aromatic properties but they possess an important biological activity. The aim of the present investigation is to examine the biological activities (describes the beneficial or adverse effects of a drug and materials on living matter) of rosemary essential oil on liver and kidney tissues. The tissues treated with different doses of essential oil of rosemary (100, 200, 300, 400) ml. Histological examination of liver tissue treated with 400 ml of rosemary revealed normal architecture with rate of stimulating cells more than the other concentrations, without dilated and congested of portal vein.


Article
In vitro propagation of Gerbera (Gerbera jamesonii Bolus)
اكثار نبات الجربرا باستخدام تقنية زراعة الانسجة النباتية

Loading...
Loading...
Abstract

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

Keywords


Article
Effect of static magnetic fields against some virulence factor of C.albicans: germ tube formation, adhesion ability and biofilm formation
أثر المجال المغناطيسي الثابت على بعض عوامل امراضية Candida albicans: تكوين انبوب الانبات ، التصاقها وقابليتها على تكوين الغشاء الحيوي

Loading...
Loading...
Abstract

In vitro, inhibitory effects of static magnetic fields in the type of two poles and negative and positive pole on some virulence factors belong to C.albicans as pathogenic microorganism was investigated in this study. According to the findings of this study static magnetic fields in the type of two poles and negative pole displayed a variable degree of inhibitory effects against this microorganism via its virulence factors such as: germ tube formation, adhesion ability and biofilm formation in which negative pole showed more strongly effect than two poles as inhibitory values ranged from 35% as germination ability, 30% adhesion ability after 3hrs of treatment and 2.2mg/disk dry weight as biofilm formation after 72hrs of treatment compared with the inhibitory values for two poles ranged from 30% as germination ability, 25% adhesion ability after 3hrs of treatment and 2.7mg/ disk dry weight as biofilm formation after 72hrs of treatment . Following our earlier demonstration, the effects of positive pole also investigated in this study as 10 % for germination ability, 10% adhesion ability after 3hrs of treatment and 3mg/ disk dry weight as biofilm formation after 72hrs of treatment. These results revealed that static magnetic fields in the type of negative pole may be useful as antifungal agent against Candida albicans.

Keywords


Article
Induction of murine malignant lymphoma by 1-(2-chloro-ethyl)-3-cyclo-hexyl-1-nitrosourea, In vitro partial characterization
استحداث سرطان اللمفوما الفاريه باستخدام الاثل نايتروزويوريا وانشاء مزرعه خلوية اولية منه

Loading...
Loading...
Abstract

From 20 mice administered ethyl nitrosourea for over 5 months, 2 mice showed lung lymphoma, one of them is a murine malignant lymphoma which cultured and the cell line was newly established as short term culture. The cells were round in shape and had a tendency to make groups of floating clusters. Later these cells developed to proliferate as adherent cell culture. Our study showed that ethyl nitrosourea is selective carcinogen to induce lymphoma and lymphoma cultured cells is very useful for lymphoma studies.


Article
Development of SYBR Green Real time PCR for identification methicillin-resistance Staphylococcus aureus (MRSA)
الكشف عن البكتريا العنقودية الذهبية المقاومة للمثسلين Methicillin-Resistant Staphylococcus aureus باستخدام طريقة التضاعف التسلسلي المزدوج SYBR Green Real time PCR

Loading...
Loading...
Abstract

The increasing resistance of staphylococci to ß -lactam antibiotics has become a major clinical problem. Development of rapid and sensitive techniques for detection of MRSA is an important aim for public health. A duplex PCR were established for specific identification of methicillin-resistance Staphylococcus aureus (MRSA) in clinical samples. In this work a duplex SYBR Green real time PCR was developed for rapid identification of MRSA in local methicillin-resistance S. aureus isolates. Twenty methicillin-resistance S. aureus isolates, as determined by disc diffusion method, were subjected to DNA extraction and PCR amplification. Two genes were amplified successfully, mecA (533bp) and femA (314bp), as targets for methicillin-resistance and specific identification of S. aureus, respectively using conventional PCR. Sensitivity of the duplex PCR showed that the minimum concentration of DNA that gave positive results for the two genes was 30ng/µl. In order to develop rapid and sensitive test for identification of MRSA, serial dilutions of purified DNA were amplified gradually according to their concentrations using SYBR Green real time PCR. These results indicated that the SYBR Green real time PCR can be used for identification of methicillin-resistance S. aureus (MRSA) in clinical


Article
Increasing serum level of transforming growth factor beta 2 (TGF-β) in patients with colorectal and gastric cancer in Iraq
إرتفاع مستوى Transforming growth factor beta2 (TGF-β) في مصل مرضى سرطانات القولون والمستقيم والمعدة في العراق

Loading...
Loading...
Abstract

Gastrointestinal cancers (GITs) are worldwide problem particularly in highly developed countries. In Iraq, gastric cancer(GC) is the 9th most common cancer while colorectal cancers (CRC) is considered as the 7th most common cancer among all cancer patients in both males and females. The Objective of this study was to estimate the serum level of transforming growth factor beta 2 (TGF-β) in Iraqi patients who are complying from gastric and colorectal cancers. Fifty four serum samples were collected starting from the 1st of March till the mid of May of 2011 to investigate the TGF-β serum level by using ELISA technique. Thirty eight samples were gastric (H.pylori +ve) and colorectal cancer patients (GC=17, CRC=21) while the other 16 samples considered as a healthy control group. The results showed that TGF-β serum levels of both GIT tumors were increased significantly (p<0.05) compared to the healthy control group. In conclusion, the presented study showed elevated serum level of TGF-β in Gastric cancer patients which could point out to use this elevation as a biomarker for tumor prognosis; while in colorectal cancer it may evade the immune system cancer killing mechanisms. We recommend further studies concerning the correlation between serum level of TGF-β in Gastric cancer patients with staging and grading, and more immunological techniques can be implied to know the exact immunological evading mechanism of colorectal cancer cells.


Article
Partial purification and characterization of a bacteriocin produced by Lactobacillus acidophilus
التنقية الجزئية وتوصيف البكتريوسين المنتج من بكتريا Lactobacillus acidophilus

Loading...
Loading...
Abstract

Bacteriocin produced by Lactobacillus acidophilus was partially purified by ammonium sulfate precipitation 60% saturation. The bacteriocin exhibited activity of 320AU/ml against Serratia marcescens. Characterization of bacteriocin showed that its active principle was proteinaceous in nature since it was inactivated by proteolytic enzymes but not by other enzymes. Treatment of bacteriocin with organic solvents 5% concentration did not affect the activity of it, but its activity was reduced to the half at 10% concentration of the solvents. Bacteriocin activity was stable at pH 4 -7, half of its activity was lost at pH8, and whole activity was lost at other pH values. Bacteriocin was stable at 40-100°C for 10min, but it retained only 50% of its activity at 40 and 60°C for 30min. whole activity was lost at 80 and 100°C for 30min and at 121°C for 15 min.

Keywords


Article
Study of the Antimicrobial effect of Melia azedarach L. plant
دراسه للفعالية ضد الجرثوميه لنبات السبحبح Melia azedarach L.

Loading...
Loading...
Abstract

This study was aimed to evaluate the antimicrobial activity of the leaf extracts of Melia azedarach L. plant against different pathogenic microorganisms such as (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, klebsilla sp., Candida albicans). Two plant extracts (Aqueous and ethanolic) under four different concentrations (25, 50, 75, 100) mg/ml were used by Agar-well diffusion method. Chemical detection of extract showed that the extracts contain tannins, flavonoids, terpins, steriods, alkaloids, and saponins. Aqueous leaf extract showed no effect against all tested microorganisms at (25, 50, 75, 100) mg/ml concentration except Candida albicans which was sensitive to 100mg/ml concentration. While Ethanolic (80%) leaf extract showed sensitivity on Staphylococcus aureus at 100mg/ml concentration.


Article
Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601.
انتاج الجذور الشعرية المحَولة وراثيا المنتجة للحامض الاميني الاكروبين (Agropine) في نباتات الحلبة بوساطة Agrobacterium rhizogenes 1601

Loading...
Loading...
Abstract

Inoculation of leaves and stems explants excised from field-grown Trigonella foenum-graecum L. with engineered Agrobacterium rhizogenes 1601 inoculum of optical density 1.90, 2.06 and 1.96 led to the formation of hairy roots on these explants. The highest percent of infection with inoculum of O.D 1.90 was 20% in leaves, and was 53.3% in stems using inoculum of O.D 2.06. Infection percent was 50% in hypocotyl compared with non-inoculated explants. The results showed that inoculation of seedlings lacking roots were slow in hairy root formation and take long time compared with their formation in intact seedlings inoculated with the same inocula. Results of paper electrophoresis of these root proved the incidence of their genetic transformation. Moreover, these transformed roots grow happily in liquid WP medium with an average fresh weight 5.9g after four weeks of culture, whereas fresh weight of normal roots was 1.02g under the same conditions.

Keywords

Table of content: volume: issue: