جدول المحتويات

المجلة العراقية لبحوث الأجنة والعقم

ISSN: 22180265
الجامعة: جامعة النهرين
الكلية: ابحاث الاجنة ومعالجة العقم
اللغة: English

This journal is Open Access

حول المجلة

المجلة العراقية للأجنة وبحوث العقم هي مجلة منشورة سنوياً من قبل المعهد العالي لتشخيص العقم والتقنيات المساعدة على الانجاب/جامعة النهرين،
وتنشر المخطوطات في مجالات الطب التناسلي.

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معلومات الاتصال

E-mail: haydermossa@gmail.com

E-mail: ijeir.info@ierit.nahrainuniv.edu.iq

Website : http://www.ijeir.nahrainuniv.edu.iq

جدول المحتويات السنة: 2012 المجلد: 2 العدد: 4

Article
Correlation between in vitro sperm preparation techniques, endometrial thickness, hormonal profile and successful pregnancy rate following IUI: retrospective and prospective study
العلاقة بين تقنيات تنشيط النطف ,عدد الجريبات وحجمها, سمك بطانة الرحم , قياس الهرمونات وبرنامج تحفيز المبايض المستخدم في عملية التنمية داخل الرحم مع معدلات الحمل :دراسة استرجاعية ومستقبلية

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الخلاصة

Background: The intra uterine insemination (IUI) still an easy, simple and effective method to overcome the infertility that resulted from different causes. Although the equipments and methods of assisted reproductive technologies (ART) were highly developed, the percentage of successful IUI live-birth not exceed 20- 30%. Objective; The present study was aimed to elucidated the cut off values of in vitro sperm preparation technique, follicular number and size, endometrial thickness and hormonal profile that lead to successful pregnancy rate (PR)following IUI. Materials and Methods: In retrospective study, one hundred cases of infertile couples were in who were became pregnant following IUI at the Institute of Infertility Diagnosis and Assisted Reproductive Technologies, through the period from January 2007 to January 2010. Depending on the results of retrospective study, IUI was achieved for 100 infertile patients between August 2010and June 2011. The mean of significant prognostic variables were measured in both studies namely: age, type of infertility, type of sperm activation techniques, the protocol of ovulation induction medicine, the mean of hormonal status of Luteinizing Hormone (LH), Estradiol(E2), and number with diameter of follicles, endometrial thickness a cycle day before hCG injection Results: The protocol of ovulation induction in IUI cycles by using clomiphene citrate(CC) and recombinant gonadotropin (rFSH) was significantly(p<0.05) improve the IUI results compared to CC alone or CC with Pergonal®. The mean values obtained by ultrasonography namely; two follicles, and dominant follicle of 19.5mm in size with endometrium thickness of >9.5mm were gave significantly successful IUI outcome compared to other values. In prospective study similar results were obtained .therefore the overall pregnancy rate was 37% per cycle. Conclusion: The calculated data of successful IUI outcomefrom retrospective study gave a best pregnancy rate in prospective study which was 37%.Consequently, these results can be considered as guideline for physician who interest in assisted reproduction to have a success in IUI.


Article
Cryopreservation of ovarian cortex and vitrification of immature oocyte
حفظ قشرة المبيض بالقرى والتجميد السريع للخلية البيضية غير الناضجة

المؤلفون: صفاء الحسني --- تهاني العزاوي
الصفحات: 15-21
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الخلاصة

Background: Unlike sperm production in men which is continuous, women are born with all their eggs and they do not produce any more. Technique to bank eggs would allow girls and women to have the same reproductive options as men when faced with a serious disease and the long term complications of chemotherapies that threatens to destroy their eggs. Objective: The aim of this study is to improve the efficiency of existing ovarian cryopreservation, by following the most recent method named needle immersed vitrification (NIV) beside the slow freezing method. In this review we discussed also the historical description of the methods used, freezing and vitrification of mature and immature oocytes, this include comparison of principles, procedures and results as reported in the literature. Materials and Methods; A total of 285 fragments of human ovarian tissues were isolated and cryopreserved from 36 women aging 26 - 55 years old in the Reproductive Unit, Lubeck-Germany. Ovarian tissue strips of 13- mm thick of ovarian cortical biopsies obtained from patients. The collected human ovarian cortex fragments were assigned to two different cryopreservation procedures, slow-freezing and NIV. On the otherhand, a total of 80 patients (100 cycles) with polycystic ovaries (PCO) were underwent in vitro maturation (IVM) treatment. The number of inspirated immature oocytes was 536. vitrification of immature oocytes were carried out. Results: Successful freezing and storing were performed on 285 fragments of ovarian cortex tissue from 36 patients by two cryopreservation protocols as mentioned before but these tissues do not thaw and use yet. Concerning the IVM, the percentage of maturation, fertilization, survival, embryo transfer, and pregnancy rates were 55.2%, 44.5%, 96.2%, 88.1%, and 10.6%, respectively. Conclusion: Recent advances in freezing technology, modifications of conventional protocols used and continuing optimization of vitrification have efficiently improved the method. These results may lead to increase the possibility of preserving fertility by cryopreservation of ovarian tissue. Ovarian tissue banking can offer hope for cancer patients who want to safe guard their fertility against sterilizing chemotherapy and radiotherapy. Immature oocytes can be successfully isolated from the oophorectomy specimen regardless of the day of menstrual cycle, and undergo IVM and cryopreservation.


Article
Evaluation of PSA tumor marker in some Iraqi women with polycystic ovarian syndrome.
تقييم المستضد البروستاتي النوعي لدى بعض النساء العراقيات المصابات بمتلازمة تكيس المبيض المتعدد

المؤلفون: زينب فائق رزيج --- نوال خيري العاني
الصفحات: 22-24
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الخلاصة

Polycystic ovarian syndrome (PCOS) is the most common cause of hyperandrogenism anovulatory infertility; it affects 5-10% female reproductive age.The present study aims to investigate the total prostate specific antigen levels, total serum testosterone, FSH and LH in women with PCOS and compare the results with control group of normal fertile females of corresponding age group on Iraqis PCOS patients. Materials and Methods: Seventy patients with PCOS diagnosed depending on three criteria: Menstrual history: Oligomenorrhea, Ultrasound reveals polycystic ovaries and Biomedical and I or clinical hyperandrogenism.Twenty normal fertile females who serve as control group in this study. Blood samples were aspirated from all individuals from 24- day of menstrual cycle to measure total prostate specific antigen(PSA), total testosterone and FSH, LH . Results: Patients with PCOS and controls differed significantly in PSA, Total serum testosterone (p<0.05). Patients with PCOS and controls have highly significant difference in LH level, the mean was (7.88+ 1.83 vs 3.90+ 0.73) respectively (p<0.001 )and, highly significant difference in LH/FSH ratio and BMI parameters. (p<0.001). No significant differences were found in FSH (p>0.05). Positive correlation between PSA and testosterone, PSA and BMI. Conclusion: Total serum prostate specific antigen levels are higher in patient with PCOS.Total testosterone levels are higher in patient with PCOS.Serum PSA measurement might be marker of hyperandrogenism in females suffering from PCOS.


Article
Serum leptin level in women with polycystic ovarian syndrome
دورة مصل اللبتين في النساء اللواتي يعانون من متلازمة تكيس المبايض

المؤلفون: بتول حسين كاظم --- وليد حميد يوسف
الصفحات: 25-28
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الخلاصة

Background: Leptin, a key hormone in energy homeostasis and neuroendocrine function, has a permissive role in the pathogenesis of reproductive dysfunction. Objective: To assess the role of serum leptin in women with PCOS and to evaluate leptin and insulin levels in PCOS women before and after treatment with metformin. Materials and Methods: Sixty women of reproductive age (18- 38years) were allocated to four groups: 15 obese women with PCOS (BMI >30 kg/m2), 15 obese controls, 15 non-obese women with PCOS (BMI 18 -30 kg/m2), and 15 non-obese controls. Serum leptin and insulin levels were measured and compared between case and control subjects also comparison done pretreatment and after treatment with metformin. Results: There was a significant increase in leptin in non-obese PCOS group(8.2+ 2.73) compared to non-obese control (5.64+1.43), (P value=0.0032), insulin level was significantly higher in PCOS group (15.87 ±6.65) than control (5.47 +1.68), (P value<0.001). There was significant decrease in BMI ,leptin and insulin levels after 12 month of metformin treatment in obese and non-obese PCOS subjects. Conclusion: Leptin level increased remarkably with increasing body weight and it is higher in non-obese PCOS women in comparison with non-obese healthy women.


Article
Role of leptin in infertile men before and after treatment with clomiphene citrate and vitamin E
دور اللبتين في الرجال العقيمين قبل وبعد المعالجة بالكلومفين ستريت وفيتامين ه

المؤلفون: وليد حميد يوسف --- احلام حمادي عيسى
الصفحات: 29-33
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الخلاصة

Background: Leptin is an adipocyte -secreted protein that participates in the regulation of energy homeostasis. It prevents the body from storing fat, controls hunger and cravings, regulates food intake and energy expenditure, provides the body with an index of nutritional status and controls the whole body fat metabolism. Leptin is a product of ob gene created by adipocytes. It seems to signal metabolic information to the reproductive system. Objective: The aim of this study is to evaluate the relationship between serum leptin and infertility in oligozoospermic men. Materials and Methods: Seventy men were investigated; fertile normozoospermic as a control (n = 35) and infertile oligozoospermic (n =35). The patients underwent estimation of body weight (kg), height (cm), body mass index (BMI), semen analysis, serum FSH,LH, testosterone and leptin, lipid profile and serum malondialdehyde (MDA). The infertile group was given a treatment course of clomiphene citrate (50 mg) and vitamin E (400mg) and then all the previous parameters were reevaluated after 3 months. Results: Mean body weight was significantly higher in infertile oligozoospermia compared to controls.The height showed no significant difference between the two groups.Hormonal profile revealed significant (P=0.0001) difference in FSH between control group and oligozoospermic group (7.04+ 4.20 versus 3.35+ 1.47 mill/ ml), but after treatment with clomiphene citrate and vitamin E there is a slight non-significant (Pz0.231) decrease in the level of FSH in oligozoospermic group.The level of testosterone showed a statistically significant (P=0.0001) difference between fertile normozoospermic (5.52 +1.29 ng/ml) and infertile oligozoospermic (2.40 ±0.96 ng/rril).This hormone showed a significant (P=0.0001) increase in its level after treatment with clomiphene citrate and vitamin E (3.48± 1.56 ng/ml). Other hormone showed non significant difference between the two groups pre and post treatment. Lipid profile showed no significant difference between the two groups. There was no significant (P=0.477) difference in serum MDA between the control and the infertile groups (4.69+ 1.32 vs. 5.18± 1.19 pmol/L) respectively,but after treatment the results showed a mild significant (P=0.026) decrease (4.30± 0.67 pmol/L) in comparison to the level in the control group and also a significant (P=0.0001) decrease in comparison to the level before treatment. Infertile oligozoospermic had significantly (P<0.0001) higher serum leptin level (8.03± 1.22 ng/ml) than control (3.62 + 1.00 ng rnl). Conclusion: Serum leptin demonstrated a significant positive correlation with age, body weight, BMI and a significant inverse correlation with serum testosterone. It had nonsignificant correlation with the height and sperm concentration. These results are suggestive of a link between the adipocyte derived hormone leptin and male reproduction.


Article
Measurement of serum Inhibin B as a predictive evaluation of ovarian response following ovulation induction program in intrauterin insemination
قياس هورمون الانهيبين (ب) في مصل الدم كمقياس تقييمي لتخمين استجابة المبايض في برامج تحفيز الاباضة المستخدمة في التقنيات المساعدة على الانجاب

المؤلفون: ليلى خضر غالب --- نوال خيري
الصفحات: 34-39
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الخلاصة

Background: The evaluation of ovarian reserve has been, and still, the focus of substantial clinical research. The assessment of ovarian reserve is valuable for determining stimulation protocols, and because of limited predictive value of age alone, or other passive hormonal analysis in estimating response to the exogenous stimulation, Dynamic research was done on serum inhibin B. Objective: Measurement of serum inhibin B as a predictive evaluation of early ovarian response following ovulation induction program in intrauterine insemination(IUI). Materials and Methods: Thirty couples were involved in IUI program. They divided into two groups: group (1) 15 women were treated by Gonadotropin(Gn) recombinant follicle -stimulating hormone (rFSH). While group (2), 15 women were treated by Clomiphene citrate (CC). Treatment started at day 3 of the menstrual cycle. Serial hormonal profile tests namely; inhibin B, Estadiol (E2), Follicle-stimulating hormone (FSH), Luteinizing hormone (LH) were done at days 3, 7 of the menstrual cycle and the day of human chorionic gonadotropin hormone (hCG) injection with recurrent ultrasonograghy (U/S) to confirm the number and development of the follicles. Results: There was a significant difference in the level of inhibin B (P< 0.05) between day3 and 7 of the cycle with the mean of the follicle number 1.67± 0.18 in group(1), while in group (2) there was a non significant difference (P>0.05) in the level of inhibin B between day 3 and 7 of the cycle with the mean of the follicle number 2.14 ±0.17. Percentage of pregnancy rate in IUI in this study was 19.23Z.Percentage of pregnancy rate in group (1) was 25% .while in group (2) was 14.28% ,but there is a significant difference (P<0.05) in the pregnancy rate between the two groups. Conclusion: It was concluded that serum irihibin B obtained at day 7(day 5 of (Gn) therapy) offers an early and accurate prediction of ovarian response to (Gn) stimulation while there was no significant change in the level of inhibin B between day 3 and 7 of the cycle in patients treated by CC .


Article
Effect of Citrullus colocynthis aqueos extract on in vitro fertilization and early cleavage stages of mice embryos: a model for mammals
تاثير استخدام مستخلص بيات الحنظل على الاخصاب في الزجاج ومعدل التطور في اجنة الفئران :كنموذج للبائن

المؤلفون: نبا عفيف --- saad s. aldujaily سعد صالح الدجيلي
الصفحات: 40-45
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الخلاصة

Background: Citrullus colocynthis(CC) is an herbal medicine used in the treatment of a wide range of diseases. Recently, the effect of this plant on the reproductive system has been studied .However, its role in vitro still unclear. Objective: The present study was designed to investigate the possibility of using CC extract for in vitro sperm direct activation technique, in vitro fertiIization(IVF) and early embryonic development using the mice as a model for mammals. Materials and Methods: Citrullus colocynthis extract (0.05mg/ml culture medium) was used for in vitro direct sperm activation technique. The same medium was used for oocytes insemination and for culturing the embryos after 2448-hours of insemination. The oocytes were collected from superovulated female mice and divided into two groups: first group, 343 oocytes were inseminated and cultured in CC- free Ham's F-12 medium (the control group).The second group, 345 oocytes were inseminated and cultured in 10% CC - Ham's F-12 medium (treated group). Each 4 oocytes were inseminated with the same sperm concentration (1 -2 ><105sperm/IVF well).The fertilization rate was recorded after 24 hours of insemination .while embryonic development rate was recorded after 24 and 48 hours of insemination. Results: In vitro activation of epididymal sperms with 10%CC has shown positive effect on sperm concentration, sperm motility, and grade activity of progressive forward movement. There was a highly significant (P<0.004) increase in FR of the treated group (61.5%) compared to control group (50.4%) after 24 hours of insemination. Embryonic developmental rate was significantly increased after 24 and 48 hours of insemination in treated group compared to control group. Conclusion: It is concluded that adding the 10%CC to the culture medium of the epididymal sperm and in vitro inseminated lead to improvements in certain sperm function parameters and sustain the FR and early embryonic development rate.


Article
Study the effects of glutathione on fertility potential in vitro of male diabetic induced mice
دراسة تاثير الكلوتاثايون على فعالية الاخصاب الخارجي لذكور الفئران المصابة بداء السكر

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الخلاصة

Background: Diabetes has been found to induce various subtle molecular changes important, for sperm quality and function: The mammalian spermatozoa are rich in polyun- saturated fatty acids, making them particularly sensitive to the deleterious effects of lipid peroxidation, which may result in irreversible loss of motility and a low level of fertilizing capacity. An antioxidant, glutathione (GSH) a polypeptide is important in biological oxidation-reduction reactions and improves sperm motility. Objective: The study was aimed to investigate the effects of GSH injection on healthy and diabetic male mice fertilization potential in vitro following in vitro fertilization (IVF) Materials and Methods: Inducing diabetes in mice through intra peritoneal alloxan injection. The treatment with GSH divided into two periods 10 and 20 days, studying the in vitro fertilization outcomes. Results: Glutathione daily IV injected in a period of 10 to 20 days for the diabetic induced male mice significantly increase the sperm concentration and motility and decrease the abnormal and dead sperms percentages in treated group compared to untreated group. The Fertilization rate was significantly (P<0.05)increased in mice treated with GSH compared to untreated one. Conclusion: Glutathione injections may decrease the oxidative stress caused by diabetes and enhance the in vitro fertilization potentials in male mice.


Article
Effect of penicillin on uterus in mice
تاثير البنسلين على الرحم في الفئران

المؤلفون: ضحى اكرم ابراهيم --- محمد باقر فخر الدين
الصفحات: 52-57
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الخلاصة

Background: Background: The reproductive failure is a significant public health concern. Although relatively little is known about factors affecting fertility, there is sufficient evidence to hypothesize that antibiotic may influence the fertility. Objeclive: This study was established to explore the individual impact of different doses of penicillin on some reproductive parameters. Materials and Methods: Forty adult female albino mice (12-18 weeks) and weight (25- 28) gm divided into four groups. Control group (G1) was treated daily with normal saline, and other three groups that treated with different doses of penicillin G2 (2mg/kg/B.wt), G3 (3mg/kg/B.wt), G4 (4mg/kg/B.wt)for four cycles twice daily (IM) injection at proestrus phase and sacrificed at estrus phase. Parameters were assessed include, thickness of endometrium, length of epithelial cells, number and diameter of uterine gland using histological section and measurement by (motic image plus). Results: The results of this study demonstrate that there is significant decrease (P<0.05) in thickness of endometrium, height of epithelial cell, number and diameter of uterine glands of female mature mice after treatment with high doses (3mg/kg/B.wt), while, there is no significant decrease (P>0.05) after treatment with (2mg/kg/B.wt); (3mg/kg/B.wt) compare to control group. Conclusion: The result showed higher doses for long period of penicillin has impact of some reproductive parameters of mature female mice.

الكلمات الدلالية

penicillin --- reproduction --- mice

جدول المحتويات السنة: 2012 المجلد: 2 العدد: 4