Table of content

Basrah Journal of Veterinary Research.

مجلة البصرة للابحاث البيطرية

ISSN: 18138497
Publisher: Basrah University
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

About

. Basrah Journal of Veterinary research publishes in Arabic or English Original and distinguished paper ,short communications, Case reports and reviews in the field of veterinary sciences. The journal is published two times a year.

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Contact info

Mobile;07801034925
E.mail;alaasawad1965@yahoo.com

Table of content: 2013 volume:12 issue:1

Article
COMPARATIVE STUDY OF HEMATOLOGICAL CHANGES AND THERAPEUTICAL EFFECT OF TOXOPLASMOSIS IN NINEVEH’S WOMEN AND SMALL RUMINANTS
مقارنة التغيرات الدموية والتأثيرات العلاجية في النساء والمجترات الصغيرة في محافظة نينوى المصابة بداء المقوسات

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Toxoplasma gondii is an important protozoan pathogen of human and animals, The study was conducted on 557 serum samples of pregnant women from Al-Battol, Al-Hansa, Hamdania Hospital and privet laboratories located in Nineveh between October 2011 to March 2012,suspected patients were in age ranged between 22 up to 41 years to detect Toxoplasma antibodies using latex agglutination test (LAT). Results showed infection rate of 32-43%, with titer ranged between 1 4 to 1 32 which indicated chronic infection.Significant decrease in TRBs, Hb and PCV were detected in pretreatment patients compared with post treatment .Moreover 312 sheep and goats serum samples were also used to identified Toxoplasma antibodies and results showed that (43.66%) , (37.64%) of goats and sheep respectively were infected with Toxoplasmosis. It have been concluded that Toxoplasmosis were and important infectious disease affected both woman and animals in Ninavah province . الخلاصة يعد داء المقوسات الكوندية من الاوالي المهمة والممرضة التي تصيب الانسان والحيوان، شملت الدراسة فحص 557 عينه من مصل النساء الحوامل جمعت من مستشفى البتول، الخنساء، الحمدانية وبعض المختبرات الخاصة الواقعة في محافظه نينوى من تاريخ 1-10- 2011 لغاية 31-3 - 2012 للنساء بعمر تراوح بين 22 لغاية 41 سنة للكشف عن اضداد داء المقوسات الكوندية باستخدام اختبار اللاتكس المباشر حيث اظهرت النتائج نسب اصابة عالية تراوحت بين 32-43%، واظهرت الفحوصات المناعية ان المعايير14 الى 132 كانت بأعلى النسب مما يدل على ان الاصابات كانت بطورها المزمن ، وعند اجراء الفحوصات الدموية ومنها قياس تركيزخضاب الدم وحجم كريات الدم المرصوصة والعدد الكلي لكريات الدم الحمر اظهرت انخفاض شديد في قيمها عند الاصابة بالطفيلي وتحسن قيمها بصوره تدريجية مع استخدام العلاج، حيث ان المجموعة الاولى من النساء المصابات عولجت بمستحضر البيرامايسين مع حامض الفولك والمجموعة الثانية عولجت البايريميثامين + تراي ميثوبريم + حامض الفولك. وقد أجريت الدراسة ايضا للكشف عن وجود الأجسام المضادة في مصل الدم من الأغنام والماعز المصابة بطفيلي المقوسات الكوندية في مستشفى باب سنجار البيطري والمستوصف البيطري في الحمدانية باستخدام اختبار اللاتكس المباشر في مصل 312 من العينات (170 الأغنام، و 142 ماعز) في نفس الفترة حيث أظهرت النتائج نسبة اصابة بلغت في الماعز (43.66٪) والأغنام في (37.64٪).استنتج من هذه الدراسة ان داء المقوسات يعد من الإمراض الخمجية المهمة والانتقالية إذ يصيب النساء والحيوانات في محافظة نينوى .


Article
PROTECTIVE EFFECT OF ETHANOLIC GINGER EXTRACT AGAINST CADMIUM TOXICIY IN MALE RABBITS
التأثير الوقائي للخلاصة الكحولية للزنجبيل ضد التسمم بالكادميوم في ذكور الأرانب

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This study was done to evaluate the hypoglycemic potentials of ginger (zingiber officinale ) in controlling hyperglycemia ,hypoinsulinemia and their effect on total cholesterol in cadmium-induced pancreotoxicity in male rabbits Twenty four of adult male rabbits were divided randomly into four equal groups. Group 1 was given distilled water orally and considered as control group, group 2 received 10mg/kg B.w of cadmium chloride, group 3 received 100mg/ kg B.w of ginger extract plus 10mg/kg B.w of cadmium chloride and group 4 received 200mg/ kg B.w of ginger extract plus 10mg/kg B.w of cadmium chloride. All treatments were given a daily dose for 35 days. The blood sample was taken from these animal groups after 35 days of treatment for biochemical analysis to estimate : insulin hormone, blood glucose ,total cholesterol, Aspartate amino transferase (AST) , Alanine aminotranferase(ALT) and Alkaline phosphatase (ALP). The results showed that cadmium chloride administration resulted in a decrease in the level of insulin concentration associated with an increase in glucose and total cholesterol concentration in rabbits .The enzyme activities of serum ALT, AST and ALP were also significantly increased . While , the groups of animal that received ginger showed significant decrease in serum glucose , cholesterol , AST, ALT and ALP. The level of insulin was also increased in these groups of animal.


Article
THE EFFECT OF TAURINE ON REPRODUCTIVE EFFICIENCY IN MALE RATS FED HIGH CHOLESTEROL DIET
تاثير التاورين على الكفاءة التناسلية لذكور الجرذان المختبرية المغذاة على جرعة عالية من الكوليسترول

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The study designed to use high cholesterol diet to male rats(Rattus norvegicus) and asses the ability of the different concentrations of taurine to protect the reproduction from the harmful effect of hypercholesterolemia during 4 weeks of treatment .Thirty six adult male rats were used, randomly divided into six equal groups (six for each)as control and five treatment groups. Control of animals were fed on the standard ration. First treated group was supplied with the standard ration in addition to 1.5% taurine . Second treated group was supplied with standard ration in addition to 1.5% cholesterol .Third, Fourth and Fifth treated groups were supplied with standard ration in addition to 1.5% cholesterol and 2,3and 4% taurine /kg ration respectively and were handled for four weeks. At the end of the experiment the blood serum samples were collect and FSH, LH, testosterone and estrogen levels were taken and sperm vitality was recorded in addition to the weight of testis and epididymis were recorded. The results revealed to the positive role of taurine in protection of reproductive from the pad effect of hypercholesterolemia in male rats .The taurine led to increase in Gonadotropin hormones FSH and LH in addition to the testosterone after it has been reduced due to cholesterol in the ration and as a results to gonadotropin and testosterone hormone improvement ,the sperm viability was improved as well after it was decline due to high cholesterol addition in experimental


Article
EFFECTS OF DEXAMETHASONE, ESTROGEN ADMINISTRATION ON LEPTIN, THYROID, REPRODUCTIVE HORMONE CONCENTRATION AND LIPID PROFILE OF FEMALE RABBITS SERUM.
تاثير اعطاء كل من الديكساميثازون وھرمون الاستروجين في تركيز كل من ھرمون اللبيتين وھرمونات الغدة الدرقية والتناسلية ومستوى دھون الدم في مصل دم اناث الارانب

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The aim of this study investigate the effects of long term administration of Dexamethasone (DEX.) 17 β-estradiol (EST.) on body weight, concentration of some hormones ( Leptin, thyroid and reproductive hormone) in addition to determination of lipid profile of female rabbits blood serum. Thirty local rabbits were subdivided randomly and equally into three groups. The first group was used as a control which injected with normal saline . The second group was injected daily (0.5mg /kg B.W) DEX . Intramuscularly. The third group was injected subcutaneously daily with 0.3 mg /kg B.W s/c 17 β-estradiol. All groups in this study were treated for 60 days , and blood collected every 15 days. Serum was tested for leptin, LH, FSH, T3, T4 hormones total Cholesterol(TC), HDL(height density lipo-protein), LDL (low density lipo- protein),TG (triglycerides), Body weight was taken every 15 days from the first day . The result showed significant (P≤0.05) decreased in body weight after administration of both dexamethasone (DEX.) and Estrogen (EST.), significant increased in leptin concentration with dexamethasone (DEX.) Treatment while significant decreased with Estrogen (EST.) treatment, in the other hand LH and FSH concentration showed significant decreased in both treatment dexamethasone (DEX.) and Estrogen (EST.) thyroid hormone show significant decreased with dexamethasone (DEX.) Treatment and significant increased T3 & T4 hormone with Estrogen (EST.) treatment as compare with control while the increase in the T4 it was occurred only in the periods (45and 60)days within treatment groups , lipid profile (TC,TG,LDL, HDL,VLDL) show significant increased with DEX. treatment while Significant decreased with EST. treatment


Article
DISTRIBUTION OF ECTOPARASITES INFESTED SHEEP AND GOATS IN DUHOK PROVINCE, NORTH IRAQ.
توزيع الطفيليات الخارجية الخراف المصابة والماعز في محافظة دهوك، شمال العراق

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An investigation into ectoparasites of sheep and goats was carried out in Duhok province North West region of Iraq, during six months of a year, from January to June 2010. One thousand and two hundred forty eight (1248) sheep and 954 goats in 110 flocks from 80 villages were examined. Among them overall animals (66.89%) were infested with one or more species of ectoparasites. Of 720 (57.7%) and of 753 (78.9%) sheep and goats, respectively were infested. Five different types of ectoparasites, ticks (46.7%, 34.9%), lice (3.8%, 33.8%), mites (7.1%, 0.1%), fleas (2.8%, 7.75%) and ked (1.2%, 4.5%) were identified sheep and goats, respectively. Five species of hard ticks (Ixodidae) were identified in both sheep and goats, namely Hyalomma anatolicum anatolicun (48.37%, 13.5%), H. marginatum (18.01%, 8.1%), Rhipicephalus sanguineous (21.09%, 39.93%), R. turanicus (16.8%, 49.54%) and Haemaphysalis ssp. (2.5%, 0.9%). Two species of lice were infested each one of animal these namely, Damalinia Ovis (75%) and Linognathus stenopsis (33.3%) on sheep, and D. caprae (80.74%) and L.stenopsis (19.2%) on goats. Sheep were highly infested with two species of mites, Sarcoptes scabiei (25.84%) and Psoroptes Ovis (74.15%) than the goats, which one goat was found (0.1%) infested with S. scabiei. Three species of fleas were found infested both sheep and goats. Out of 106 collected fleas (47.2%, 43.4%, 9.4%) were Ctenocephalides felis felis, Pulex irritans and Xenopsylla cheopis, respectively. However, the only one species of Ked Melophagus ovinus were infested (1.2%) of sheep and (4.5%) of goats.

Keywords

ectoparasites --- Duhok --- Goat.


Article
CROSS AND RADIOLOGICAL STUDIES OF THE SALIVARY GLAND IN CATTLE
دراسة عيانية وشعاعية للغدد اللعابية في الابقار

Authors: S . AL Sadi سمية الساعدي
Pages: 65-75
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The present work includes morphological and radiological studies of the salivary gland , saliva is the mixed secretion of these gland the secretion of saliva in ruminants is continuous ,it has been moistest oral mucosa, provide medium for dissolved food and control bacterial flora of the oral cavity , the aim of present work to report more detailed information about the salivary gland and duct in cattle which may be help in both anatomy and surgery aspect, for value impartment can easily removal all salivary gland tissue during surgical operation, the large salivary ducts occasionally cannulated to remove obstructions or to inject a contrast medium for radiographic examination and to be able to palpate the parotid and mandibular salivary gland and different the mandibular salivary gland from lymph nodes ,to be able to trace the ducts of the salivary glands and palpate the parotid duct in the cheek of cattle , to be extirpate the mandibular and monostomac sublingual salivary glands . Collected from (6) fresh preserved heads were used in this study, there were equally divided into two groups : first group to study of shape ,position and relation of major salivary gland also study the morphology of the minor salivary gland and second group study morphological and radio graphical of parotid , mandibular and sublingual duct in cattle.The study revealed that the three major paired salivary glands(parotid ,mandibular and sublingual gland ) in addition four minor salivary glands ( buccal ,lingual ,palatine and labial gland ) into the oral cavity and oropharynx in cattle the mandibular gland ,unlike that of other domestic animals , the mandibular salivary gland is larger than the parotid ,distinctly lobulated and lies in the curve along the medial side of the angle of the mandible and which divides into superficial and deep loop, is easily palpate in the inter mandibular space, the duct open in the sublingual caruncle , the parotid gland has been described having (5) processes ( three superficial and two deep ) ,sublingual gland this is smallest of the major salivary gland , sometimes consists of two parts (compact and diffuse ) it is the almond shaped gland lies deep to the floor of mouth ,un like the parotid and mandibular gland the sublingual gland has no true facial capsule also it has a single duct.Un like the major salivary gland, the minor salivary gland lack a branching network of draining ducts , buccal glands are well developed and arranged in three groups (dorsal, middle and ventral )in cattle , the lingual gland are small lobules under the mucosa and embedded in the musculature, the caudal third of the hard palate is not ridged and bears numerous orifice of the palatine gland ,the labial glands a compact mass near the angle of the mouth , the most commonly occurred radiological findings using surface landmarks parotid duct lies mid way between the facial tubercle and corner of the mouth ,the ducts of the mandibular and compact (monostomatic) sublingual glands open on the floor of the mouth at the sublingual caruncle, they run below the mucous membrane that connects the side of the tongue with the gums.


Article
EFFECT OF VITAMIN C AND ZINC ON SOME BIOCHEMICAL PARAMETER IN ALLOXAN INDUCED DIABETIC RABBITS
تأثير فيتامين سي والزنك على بعض المعايير الكيموحيوية في الأرانب المصابة بداء السكري المستحدث بالالوكسان

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Diabetes mellitus (DM) a disease which is characterized by hyperglycemia, lipoprotein abnormalities and oxidative stress. This study was undertaken to investigate the beneficial effect of vitamin C and Zinc on some biochemical parameter when induced diabetic in rabbits by alloxan. Male rabbits groups divided into four groups: Group I (Negative control) received normal saline orally, alloxan diabetic rabbits : Group II (Positive control) received normal saline orally , while Group III were orally administered 50 mg/kg body weight of vitamin C and group IV were orally administered 100 mg/kg body weight Zinc for 15 days. The results showed that vitamin C administration and zinc significantly (P< 0.05) reduced the serum glucose, total cholesterol, triglyceride, ALT and AST concentration, while there were significant changes (p< 0.05) was observed on the serum levels of total protein, albumin, globulin and albumin/ globulin ratio in diabetic group treated with vitamin C, Zinc respectively.

Keywords

Diabetes mellitus --- Rabbits --- Alloxan


Article
DIAGNOSTIC STUDY OF EHRLICHIOSIS IN CATTLE OF MOSUL-IRAQ
دراسة تشخيصية للايرليخيوسز في الماشية في مدينة الموصل –العراق

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Ehrlichiosis (Ehrlichia spp.) was detected in 132(70 Iranian,32 Turkish and 30 local breed)calves (10-14 months old) and 18 dairy cows of local breed (3-5 years old). Animals were brought to Veterinary Teaching Hospital, College of Veterinary Medicine, University of Mosul –Iraq from different farms of Mosul city-Iraq, with clinical signs of fever(40-41C0),anorexia ,emaciation, decrease of milk yield in dairy cows ,paleness of mucous membrane, lymph node enlargement and ticks were detected on different body regions .All blood and buffy coat smears of calves were positive for morula like structures inside the cytoplasm of neutrophils , lymphocytes and monocytes when staining by Giemsa and Wright stains. For all animals , the appearance of Ehrlichia spp. Organisms in leukocytes coincided with erythropenia, leukopenia, and thrombocytopenia and a decrease in hematocrit and hemoglobin concentration. For eighteen dairy cows, Ehrlichia spp. organisms were identified in leukocytes of blood and milk samples. This is apparently the first evidence for Ehrlichia spp. infection in cattle in Iraq. In conclusion, the present study documents that Ehrlichia infection exists in Mosul-Iraq and indicates that the bacterium has been present but unnoticed in the area .Further investigation will be needed in order to characterize different species of Ehrlichia infection in cattle, and explain the role of vector and imported animals in introduction of the disease to our country

Keywords

Ehrlichiosis --- milk. Cows


Article
DETECTION OF BOVINE TRICHOMONIASIS OF BULLS IN BASRAH SLAUGHTERHOUSE
الكشف عن الترايكوموناسز البقري في العجول في مجزرة البصرة

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Bovine trichomoniasis is a venereal disease of cattle caused by the protozoan Trichomonas fetus . The study based on collection and diagnosis 200 samples of preputial wash from bulls that send to the Al-Tuaisa slaughter house in Basrah private by made a slide smear stained with Gemza stain . The study starts at 2011 and extend to 2012 . The result shows that the percentage of infection in bulls that slaughtered in a slaughter house of Basrah private was 2% .


Article
HISTOPATHOLOGICAL STUDY OF SEPSIS EXPERIMENTALLY INDUCED BY CECAL LIGATION AND PUNCTURE IN RATS
د ا رسة مرضية نسجية للانتان المحدث تجريبياً بواسطة ربط الاعور وثقبه في الجرذان

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The aim of this study was to investigate the histopathological lesions in the liver,kidney and lung sections in rats with sepsis that experimentally induced by cecal ligation and puncture CLP procedure to induce intra-abdominal infection. It was found that CLP models leads to higher mortality rate comparisons to sham-operated and un– operated animals. Also there were severe lesions in the liver , kidney and lung of rats with sepsis , characterized by severe massive necrosis in hepatic cells , while the histopathological changes in kidney of CLP rats revealed severe glomerulonehritis with infiltration of inflammatory cells , in lung , severe interstitial and bronchial pneumonia , with presence of severe thickening of blood vessel wall with thrombi . In conclusion, the results of this study have demonstrated that CLP induces high mortality rate , an acute systemic septic state with multiple organ dysfunction especially the Liver , kidney and Lung based on histopathological observations . These features were not observed in sham - operated or un-operated rats .

Keywords

sepsis --- CLP --- glomerulonehritis


Article
HISTOPATHOLOGICAL STUDY OF QUAILS LIVER EXPERIMENTALLY INDUCED BY AFLATOXIN
دراسة مرضية نسجية لكبد السمان المعرض تجريبيا لسموم الافلا

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This study to evaluate the toxic effects of aflatoxin (AF) at a rate of 2.5 and 5 ppm on liver histopathological appearance of local quails.sixty quail chicks at one day old randomly divided into two pen replicates of ten chicks each were assigned to each of three dietary treatments. 1) basal diet containing no AF/kg diet (control); 2) basal diet supplemented with 2.5 ppm diet and 3) basal diet supplemented with 5 ppm diet. The chicks were individually weighed on a weekly basis, feed consumption and body weight gain were recorded weekly. The trial period was 5 weeks and quails were maintained on 24-h continuous light schedule and ad libitum access to diets and water. Feeding Aflatoxin to quails included liver fatty changes, necrosis, bile duct hyperplasia and aggregation of lymphocyte . The intensity of these changes were increased with increasing AF level in the diet. The results indicated that local quails are sensitive to AF

Keywords

aflatoxin --- quail --- liver.


Article
MORPHOMETRIC AND DISTRIBUTION OF COLLAGEN FIBERS IN DERMIS OF LOCAL CANINE SKIN IN BASRAH PROVINCE
دراسة شكلية قياسية لتوزيع الالياف الغراوية في ادمة جلد الكلاب المحلية في محافظة البصرة

Authors: Sawsan A. Ali سوسن عباس علي
Pages: 127-134
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The present study was carridaed of normal skin of local dogs canins lupus familiaris age of animals was ( 8 -24 mounths). The range and distribution of collagen fibers was studed. The results showed that the posterior limbs recorded the highest values for distribution and thickness compare with abdomen region. Also the results confirmed that the orientations of collagen fibers were variations between the regions because of stress factors.


Article
STUDY THE EFFECT OF AGE AND SEX ON SOMEHAEMATOLOGICAL PARAMETERS IN GOLDEN LOCAL QUAIL
دراسة تأثير العمر والجنس على بعض المعايير الدموية في السمان المحلي الذهبي

Authors: Muhammad H.K محمد حميد خلف
Pages: 135-141
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Effect of age and sex were measured during and before sexual maturation of golden local quail males and females. Maels erythrocytes count showed higher value compared with that of adult females . Haemoglobin and haematocrit of adult males were significantly higher than those of adult females. However total leukocyte count and H/L ratio of the adult female were higher than those of males. Body weight of both sexes increased with age. As well as many of the haematological parameters differed significantly (P<0.05) in accordance to the age and sex .


Article
ANTIBACTERIAL ACTIVITY OF PIPER CUBEBA LINN. FRUIT EXTRACTS AGAINST SELECTED BACTERIAL PATHOGENS IN BASRAH CITY
الفعالية الضد البكتيرية لمستخلصات نبات piper cubeba على ممرضات بكتيرية معزوله في مدينة البصرة

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ﺺﻠﺨﺘﺴﻣ ﻲﻫﻭ (Piper cubeba) ﺔﺑﺎﺒﻜﻟﺍ ﺕﺎﺒﻧ ﺭﺎﻤﺜﻟ ﺕﺎﺼﻠﺨﺘﺴﻣ ﺔﻌﺑﺭﺃ ﺮﻴﻀﺤﺗ ﻢﺗ ﺔﺳﺍﺭﺪﻟﺍ ﻩﺬﻫ ﻲﻓ ﻩﺬﻫ ﻪﻳﻮﺘﺤﺗ ﺎﻣ ﻦﻋ ﻒﺸﻜﻟﺍ ﻢﺗ .ﺔﻴﻜﻴﻧﺎﻜﻴﻤﻟﺍ ﺔﺑﺍﺫﻹﺍ ﺔﻘﻳﺮﻄﺑ ﺩﺭﺎﺑ ﻲﺋﺎﻣﻭ ﻲﻣﺭﻮﻓﺭﻮﻠﻛﻭ ﻲﻧﻮﺘﻴﺳﺍﻭ ﻲﻟﻮﺤﻛ ﻮﻤﻧ ﻂﻴﺒﺜﺗ ﻲﻓ ﺕﺎﺼﻠﺨﺘﺴﻤﻟﺍ ﻩﺬﻬﻟ ﺔﻳﺮﻴﺘﻜﺑ ﺪﻀﻟﺍ ﺔﻴﻟﺎﻌﻔﻟﺍ ﺭﺎﺒﺘﺧﺍ ﻢﺗ ﻚﻟﺫ ﺪﻌﺑ ﺔﻴﺋﺎﻴﻤﻴﻛ ﺕﺎﺒﻛﺮﻣ ﻦﻣ ﺕﺎﺼﻠﺨﺘﺴﻤﻟﺍ ﺔﺜﻟﺎﺜﻟﺍﻭ Pseudomonas aeruginosa ﻭ Escherichia coli ﻡﺍﺮﻛ ﺔﻐﺒﺼﻟ ﺔﺒﻟﺎﺳ ﻦﻴﻨﺛﺍ ﺎﻳﺮﻴﺘﻜﺑ ﻉﺍﻮﻧﺍ ﺔﺛﻼﺛ ﺮﻴﺛﺄﺗ ﺎﻬﻟ ﻥﺎﻛ ﺔﻌﺑﺭﻻﺍ ﺕﺎﺼﻠﺨﺘﺴﻤﻟﺍ ﻥﺍ ﺞﺋﺎﺘﻨﻟﺍ ﺕﺮﻬﻅﺍ Staphylococcus aureus ﻲﻫﻭ ﻡﺍﺮﻛ ﺔﻐﺒﺼﻟ ﺔﺒﺟﻮﻣ ﻲﻣﺭﻮﻓﺭﻮﻠﻜﻟﺍ ﻭ ﻲﻧﻮﺘﻴﺳﻻﺍﻭ ﻲﻟﻮﻧﺎﺜﻳﻻﺍ ﺺﻠﺨﺘﺴﻤﻠﻟ ﺔﻴﻠﻀﻓﻻﺍ ﺖﻧﺎﻛ ﺚﻴﺣ ، ﺔﺳﺍﺭﺪﻟﺍ ﺪﻴﻗ ﺎﻳﺮﺘﻜﺒﻟﺍ ﻰﻠﻋ ًﺎﻄﺒﺜﻣ ﻭ E.coli ﺎﻳﺮﺘﻜﺑ ﻰﻠﻋ ﻲﺋﺎﻤﻟﺍ ﺺﻠﺨﺘﺴﻤﻟﺍ ﺮﻴﺛﺄﺗ ﻥﺎﻛ ﺎﻤﻨﻴﺑ S. aureus ﺎﻳﺮﺘﻜﺒﻟﺍ ﺪﺿ ﻲﺋﺎﻤﻟﺍﻭ In this study extracts of Piper cubeba fruit were prepared using mechanical method. These extracts include alcoholic, acetonic, chloroformic and water extract. The chemical composition of each extracts were analyzed also. After that the antibacterial activity of these extracts were tested against gram negative (G-) Escherichia coli, Pseudomonas aeruginosa and gram positive (G+) Staphylococcus aureus. All extracts show antibacterial activity on these bacteria, but ethanol and acetone extracts were show the best antibacterial activity S. aureus, followed by chloroform extract and then water extract, on the other hand water extract showed inhibition activity against E.coli and P. aeruginosae followed by ethanol, acetone and chloroform extract. MIC was determined for each extracts against the three pathogenic bacterial strain.


Article
MORPHOLOGICAL AND HISTOLOGICAL STUDY FOR LIVER IN LOCAL COOT BIRDS FULICA ATRA
دراسة شكلية نسيجية للكبد في طائر الغرة البيضاء المحلي

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The results of this study showed that the liver of coot bird fulica atra is closely resample to the liver of fowl that it lies in the ventral part of the body cavity and seems red-brown in color and divided into two lobes, right and left . Histologically the liver of coot bird enclosed by thin capsule of connective tissue that continue to subdivided the liver into lobules . The hepatocyte which arranged radially around the central vein as hepatocord in two cells thickness . These cells are polygonal in shape and have rounded nucleus and there is present of sinusoids between hepatocords which lined by flattened endothelial cells .

Keywords

Coot bird --- liver --- lobules


Article
EXPERIMENTAL INFECTION OF CALVES BY GIARDIA LAMBLIA CYST ISOLATION FROM HUMAN
الإصابة التجريبية للعجول بطفيلي الجيارديا المعزول من البشر

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Conducted an experimental study on the possibility of a calf infection by Giardia parasite isolated from human. Six calves were selected from the field of the Faculty of Veterinary Medicine at the University of Basrah and were divided into two groups, the first was a control group consisting of 2 calves (n=2) , and the second was group that infected and composed of four calves (n=4) . After the experiment done the percentage of infection was 100%. This study determined the amount of the dose that can cause infection to the calves and are (7- 21) cysts which were approach to those causing infection to human. The study also identified the incubation period for the giardiasis disease in calves, which were (7-10) days. This study is the first one in the Basrah and Iraq.


Article
A COMPARATIVE STUDY OF GRASS CARP (CTENOPHARYNGODON IDELLA) WHEN CULTIVATED IN POLLUTED AND NON POLLUTED AQUATIC ENVIRONMENT
175 دراسة مقارنة بين معايير الدم لاسماك الكارب العشبى المستزرعة فى بيئة مائية ملوثة و اخرى ملوثه.

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Fourty fresh water grass carp fish weighing about (35.75 ± 0.60) g were obtained from fish aquaria by, Marine Science Center, University of Basrah, Iraq. First the. Fish were individually examined for external necrosis and infections. Healthy and see it very active in the study. The physiochemical characteristics of water used for acclimation in control and polluted water that were used for the fish of experiment were daily recorded. The results of the present study show that the blood parameters ( RBC and WBC counts and Hb content) for fresh water grass carp fish were greater than those values of polluted water grass carp fish and also show increased number of dead fish among polluted grass carp, in reverse of fresh water ones. Physicochemical characteristics of water did not affect the result

Keywords

grass carp --- necrosis


Article
LEPTIN EFFECTS ON , THYROID HORMONES AND SOME BIOCHEMICAL PARAMETERS OF FEMALE RABBITS (LEPUS CUNICULUS) SERUM.
تاثير اعطاء هرمون اللبيتين على مستوى هرمونات الغدة الدرقية وبعض المعايير الكيميوحيوية لمصل دم اناث الارانب

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Abstract

Thirty local female rabbits were divided randomly and equally into three groups. The 1st group used as control which injected with phosphate buffer salin. The 2nd group was injected daily with leptin (5μg/animal) S/c. The 3rd group was injected subcutaneously daily with (10μg/animal) S/c. All groups were treated for 60 days, and blood samples were collected every 15 days, serum was tested for T3, T4 hormones, total Cholesterol (TC), HDL (height density lipo-protein) , LDL (low density lipo- protein), TG (triglycerides) and Body weight was taken every 15 days during the experiment . The result show significant (P≤0.05) decreased in body weight after administration of leptin in two doses. Significant increased in Thyroid hormone after leptin administration. While lipid profile (TC, TG, LDL, HDL,VLDL) show significant decreased with leptin treatment(5 and 10μg/animal) S/c.

Keywords

rabbits --- T4 hormones --- Cholesterol


Article
THE EFFECT OF BISMUTH CHLORIDE ON SOME BLOOD AND BIOCHEMICAL PARAMETERS IN MALE LABORATORY RATS (RATTUS-RATTUS)
تأثير كلوريد البزموث على بعض المعايير الدمية والكيموحيوية في ذكور الجرذان المختبريه Rattus-Rattus

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The present study amid to characterized the potential toxic effects of bismuth chloride through oral administration on blood and biochiemical parameters of laboratory rats.Solutions of bismuth chloride were chronically feed by stomach tube to rats in(2.5mg/kg and 5 mg/kg).Animals were anesthetized after two months and blood samples were obtained,blood and biochemical parameters were appreciated between control and experimental Groups.The investigation of blood parameters included red blood cells count (RBC) hemoglobin concentration,packed cell volume PCV and total white blood cell count(WBC)biochemical parameters included total serum cholesterol(TSCH),alanin aminotransferase (ALT) and aspartate aminotransferase(AST).The results showed significant decrease (p≤0.05) of R.B.C count ,Hb concentrations PCV value and total W.B.C count in contrast ,there was significant increase(p≤0.05) of the total serum cholesterol(,ALTand AST) activities.

Keywords

bismuth --- rats --- blood parameters


Article
PATHOLOGICAL STUDY OF LETHAL CONCENTRATION OF N-ZNO IN COMMON CARP CYPRINUS CARPIO L.
د ا رسة مرضية للتركيز المميت لصغائر اوكسيد الخارصين في اسماك الكارب الشائع Common carp (Cyprinus carpio L.)

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The lethal concentration of N-ZnO was determined in this study ,juvenile Cyprinus carpio L.mean weight 150±10g which were exposed to different concentration of N-ZnO (10-50 PPM) for 24 hrs ,the mortality rate of fish reached to 100% at 50 ppm concentration while the percentage decreased to 50% when the fish exposure to 30ppm concentration of N-ZnO.The histopathological lesions in the kidney were characterized by hemorrhage in an interstitial nephrotic tissue ,infiltration of inflammatory cells , there was cloudy swelling in the renal tubules . In the gill there were hyper trophy of pillar cells and necrosis in the secondary gill filaments .There were necrosis in the hepatic tissue and hemorrhage and sloughing in the pancreatic tissue.

Keywords

mortality --- necrosis --- N-ZnO.


Article
IDENTIFICATION OF LANCIFIELD SEROGROUP G STREPTOCOCCUS CANIS BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS (PCR-RFLP) OF 16S RIBOSOMAL RNA GENE
( Streptococcus canis تحديد المكورات السبحية لجين (RFLP) و ال PCR بواسطة سلسلة تفاعل البلمره (Lancifield serogroup G 16S ribosomal RNA

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In this study S. canis and 12 various species and serogroups of streptococci including: S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G), S. uberis, S. parauberis, S. phocae, S. suis, S. equi subsp. equi, S. equi subsp. zooepidimicus, S. porcinus and S. pyogenes were used and identified reliably by PCR-restriction fragment length polymorphism analysis (PCR-RFLP) of 1.43 kb of 16S ribosomal RNA gene using universal oligonuclotide primers and subsequent digestion with the restriction endonucleases including RsaI, MspI and AvaII. The PCR-RFLP results showed that RsaI restriction RFLP pattern of S. canis appeared different with all streptococci species baring the S. equi subsp. equi and S. equi subsp. zooepidimicus. The MspI restriction RFLP pattern of S. canis could be differentiated from S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroups C and L), S. dysgalactiae subsp. equisimilis (serogroups G), S. phocae, S. suis, S. porcinus and S. pyogenes. The AvaII restriction RFLP pattern of S. canis could be distinguished from S. dygalactiae subsp. dysgalactiae (serogroup C and L), S. dygalactiae subsp. equisimilis (serogroup G) S. parauberis, S. phocae and S. suis. In conclusion, PCR-RFLP method using restriction endonucleases RsaI, MspI and AvaII could be useful method for identification of S. canis from S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G) and other related streptococci species. It can be concluded that RFLP method might help to determine the prevalence of S. canis in animal and human infections .


Article
EFFECTS OF ETHANOLIC GINGER EXTRACT ON OXIDATIVE STRESS AND SOME TRACE ELEMENTS IN CADMIUM – INDUCED TOXICITY IN MALE RABBITS
تأثيرالمستخلص الكحولي للزنجبيل على الإجهاد التأكسدي وبعض العناصر النادرة في ذكور الأرانب المعرضة للكاميوم.

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This study was conducted to evaluate the antioxidant activity of ginger (zingiber officinale) in preventing oxidative stress ,lipid peroxidation and changes in trace elements induced by cadmium toxicity in male rabbits . For this purpose twenty four of adult male rabbits were divided randomly in to four groups ( 6 in each). Group 1 was given distilled water and considered as control group, group 2 received ( 10mg/kg B.w) of cadmium chloride , group 3 received ( 100mg/ kg B.w) of ginger extract plus (10mg/kg B.w) of cadmium chloride , group 4 received (200mg/ kg B.w) of ginger extract plus of cadmium chloride(10mg/kg B.w) . All treatments were orally given a single dose for 35 days. The water and food were provided add libitum. After the end of the treatment blood samples were taken for biochemical analysis to estimate serum Malondialdehyde ( MDA) , Antioxidant activity (Glutathione , Superoxide dismutase and Catalase ) and Trace elements (Zinc, Copper and Iron ) . The result showed that the administration of cadmium chloride resulted in a high concentration of malondialdehyde ( MDA) and caused a significant decrease in both superoxide dismutase and catalase and reduced glutathione. Serum levels of trace elements were also significantly decreased. While , ginger extract administration and cadmium chloride increased and restored their levels to near normal in comparing with cadmium chloride treated rabbits.

Keywords

antioxidant --- cadmium --- rabbits


Article
STUDY THE BIOCHEMICL EFFECT OF GUM ARABIC IN LIVER INJURY AND BLOOD SERUM OF MICE INDUCE BY GENTAMICIN
دراسة تأثير الكيموحيوي للصمغ العربي في أكباد ومصل الدم لذكور الفئران المصابة بالتسمم الكبدي المحدث بالمضاد الحيوي جنتاميسين

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Forty adult male Bulb/c mice weighing between 25-30 GM reared in wire cages were randomly divided into four groups (10 mice each group). Group (G1) received the regular mice diet and (1 ml/kg Saline p.o.) for 8 days maintained as a control group. Group (G2) treated daily intra peritoneal (i.p) injection of gentamicin (40m g/kg bwt /day) for 8 days. In group (G3) received Gum Arabic (GA) (10 gm /kg bwt /day) for 8 days orally by using stomach tube. Group (G4) is received gentamicin (40 mg/ kg bwt /day) (i.p) plus (10gm /kg bwt / day) orally of Gum Arabic for 8 days. Blood samples were collected after overnight fasting 12-24 hours for determination some biochemical marker including AST, ALT and ALP activities, creatinin, uric acid , urea and total bilirubin in serum . Assay the total protein, Deoxyribonucleic acid DNA, ribonucleic acid RNA in liver tissue of mice. The results revealed that treatment with gentamicin (G2) increased in AST, ALT and ALP activities in serum, creatinine, uric acid, urea and total bilirubin value in serum also there are elevation the level of DNA, RNA and total protein in liver tissue when a compared with the control group (G1).Treatment of GA along with gentamicin (G4) and administration of GA alone (G3) decreased in the level of serum creatinine, serum uric acid, serum urea and serum total bilirubin , the level of DNA, RNA and total protein in liver tissue also decrease, as compared to the gentamicin treated group (G2) but not reach in both groups(G3,G4) to normal level as in control group. In conclusion Gum Arabic exhibit hepatoprotective activities probably through free radical scavenging properties. Altered level of hepatic markers such as AST, ALT, and ALP with gentamicin exposure was reversed towards normalization with Gum Arabic. Similarly, biochemical parameters were also restored by Gum Arabic. Bioactive of Gum Arabic ameliorated the oxidative damage and had increased the regenerative and reparative capacity of liver.

Keywords

Gentamicin --- Stomach tube --- Uric acid


Article
MORPHOLOGICAL STUDY OF ADRENAL GLAND IN PRENATAL AND POSTNATAL PERIODS OF DOMESTIC RABBIT (ORYCTOLAGUSCUNICULUS)
دراسة شكلية للغدة الكظرية في الفترة المحيطة بالولادةللارانب المحلية

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The developmental study of adrenal gland has been done on the local rabbit fetuses and neonate, which including a morphological and histological description of the adrenal gland. The study revealed that the differentiation of the adrenal gland began in the rabbit between twenty two and twenty four days from pregnancy as adrenal cortex and medulla and relatively completed it is histologically pictures at twelve days old. Also the study showedthat the adrenal cortex composed of three zones: zonaglomerulosa or the zonaarcuata , the zona fasciculate and the zonareticularisglomerulus, whilethe medulla have dark and light cells.The purpose of our study was to provide a more complete quantitive description of the histomorphology of the adrenal gland in rabbits during prenatal and postnatalperiods.

Keywords

Adrenal gland --- fetuses --- pregnancy


Article
THEILERIOSIS IN NEWBORN CALVES IN MOSUL, IRAQ
داء الثايليريا في العجول حديثة الولادة في الموصل- العراق

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The objective of the present study were to evaluate hemogram, acute phase response, acid base balance and blood gas analysis in newborn calves infected with Theileria annulata . The study was conducted on 55 local newborn calves (male and female), 1-9 days old. The study was carried out in Mosul , Iraq. Forty five newborn calves were infected with Theileria annulata. and Ten clinically normal calves served as controls. Diseased calves showed sings of fever ,unilateral or bilateral visible swelling of superficial lymph nodes. pronounced upward bulging of temporal fossa, outward bulge of conjunctivae with exophthalmos, paleness and/or icteric mucus membranes with petechial hemorrhages on the conjunctiva, lacrimation, dyspnea and depression. There was a significant reduction in the mean values of erythrocytes count, Hb and PCV,in diseased newborn calves infected with Theileria annulata and anemia was of Macrocytic hypochromic type. The percentage of hemoparasitism (parasitemia) ranged between (3-18%) with a mean of (11.88%). Results also indicated significant increase in total leukocytes count as results of significant increase lymphocytes. Diagnosis of Theileriosis were confirmed by microscopical examination of Giemsa stained lymph node aspirate and peripheral blood smears. Results were also showed significant decrease in blood pH,Pco2,Bicarbonate, Base access and Oxygen saturation percent(So2) ,However significant increase in Anion gab was found and Titritional metabolic acidosis were indicated. Moreover results show significant decrease in haptoglobin and fibrinogen in diseased newborns calves than in controls.It have been concluded that Theileria annulata infection in newborn calves resulting in serious effects and health deterioration, leading to substantial significant economic losses


Article
STUDY THE EFFECT OF EXOGENOUS ESTROGEN ADMINISTRATION ON PROSTATE GLAND OF LABORATORY WHITE MICE (MUS MUSCULAUS)
دراسة تاثير الحقن الخارجي للاستروجين على غدة البروستات في الحيوانات المختبرية البيضاء

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A substantial advance in our understanding on the estrogen signaling occurred in the last decade. Estrogens interact with two receptors, ESR1 and ESR2, also known as ERα and ERβ, respectively. ESR1 and ESR2 belong to the nuclear receptor family of transcription factors. 24 male mice divided into four groups as following : negative control, low dose group, intermediate dose group and high dose group, the groups of treatment were injected with estrogen at different doses while the negative control group were injected with normal saline (by method of intramuscular injection for all groups) and after 6 weeks all animals had been killed and the prostate was picked up and sectioned to reveal the histopathological changes. the study found that the injection of exogenous estrogen result in marked hyperplasia of the prostatic tissue and the hyperplasia increased significantly with elevation of the estrogen dose. In conclusion injection of exogenous estrogen lead to prostate hyperplasia through its action on the estrogen receptors which present in both stromal and epithelial component of the prostate

Keywords

estrogen --- ESR1 --- prostate


Article
EFFECT OF OMEGA3+VITAMIN E ON EXPERIMENTAL INFECTION OF LABORATORY MICE WITH PSEUDOMONAS AERUGINOSA AND KLEBSIELLA PNEUMONIAE
تأثير ألأوميغا – 3 وفيتامين ه على أصابة الفئران المختبرية التجريبية بجراثيم Klebsiella pneumoniae وPseudomonas aeruginosa

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In present study, 40 adult male mice were used and divided into 5 equal groups of 8 mice each. The first group was the control group, in which the mice were fed with standard ration along the period of experiment; the second and third groups were fed on fats free ration for 14 days while the fourth and fifth groups were fed on Omega3+Vitamin E supplemented ration for 14 days too. The test organisms were suspended in phosphate buffer solution (PBS) and 10-folds serial dilutions were done for (CFU) count. Then, the animals of the second and fourth groups were injected intramuscularly with 22×105 (CFU) of Pseudomonas aeruginosa bacteria, while the animals of the third and fifth groups were injected intramuscularly with 18×106 (CFU) of Klebsiella pneumoniae bacteria. After 24 hours all mice of the third group and 3 mice of the fifth group died; their thigh muscles, livers, lungs, spleens and stomachs were taken, weighed and homogenized with phosphate buffer solution (PBS) to count the (CFU) in these organs. 2 days later, the remainder 5 mice of the fifth group were euthanized and the previous processes were done. For the second and fourth groups, after 48 hours of the bacterial injection all mice of the second group and 4 mice of the fourth group died, the previous processes were done for the bacterial count. 2 days later the remainder 4 mice of the fourth group were euthanized for the bacterial count purpose. The results revealed that the use of Omega3+Vitamin E as a supplements to ration increased the survival of mice and the organs bacterial count of the Omega3+Vitamin E fed mice decreased significantly as compared to those of the fats free fed animals at (P≤0.05).


Article
UTILIZATION OF DIET CONTAINING POULTRY EXCRETA BY CATTLE AND BUFFALO RUMEN MICROORGANISMS IN VITRO
الاستفادة من العلائق الحاوية على فضلات الدواجن من قبل الاحياء المجهرية لكرش الابقار والجاموس مختبريا

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A trail was conducted to determine utilization of diet containing poultry excreta with different sources of nitrogen by cattle or buffalo rumen microorganisms in vitro. The aim was to find alternative sources of protein and low prices ruminant diets. Commercial broiler house excreta were used as 10% of a ration. Supplemented diets were basal diet with 10% poultry excreta alone or either with 3 g/kg yeast or 1.5 g urea/kg feed or both as well as 2% molasses. The basal diet comprised 40% concentrates and 60% wheat straw on dry matter (DM) basis as a control diet. All diets were inoculated with rumen fluid of cattle or buffalo. Digestibility of DM was not significantly influenced by both species and diets. However, organic matter (OM) digestion varied significantly with different diets, the highest value was obtained by yeast, urea, poultry excreta and molasses group (64.70%). Species and diets showed significant differences in neutral digested fiber (NDF) digestion. The highest values were obtained by buffalo and the yeast, urea, poultry excreta and molasses group (65.09%). Ammonia production was significantly increased by adding urea to the diet (12.64 and 13.79). Total volatile fatty acid and acetic acid % were produced significantly higher by cattle and the group of poultry excreta only. A buffalo rumen fluid produced higher propionic acid% and less acetic: propionic percent than cattle. The total number of microorganisms influenced significantly by diet only, as yeast, urea, poultry excreta and molasses group obtained the highest number.

Keywords

poultry --- Ammonia --- wheat straw

Table of content: volume:12 issue:1