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المجلة العراقية لبحوث الأجنة والعقم

ISSN: 22180265
الجامعة: جامعة النهرين
الكلية: ابحاث الاجنة ومعالجة العقم
اللغة: English

This journal is Open Access

حول المجلة

المجلة العراقية للأجنة وبحوث العقم هي مجلة منشورة سنوياً من قبل المعهد العالي لتشخيص العقم والتقنيات المساعدة على الانجاب/جامعة النهرين،
وتنشر المخطوطات في مجالات الطب التناسلي.

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معلومات الاتصال

E-mail: haydermossa@gmail.com

E-mail: ijeir.info@ierit.nahrainuniv.edu.iq

Website : http://www.ijeir.nahrainuniv.edu.iq

جدول المحتويات السنة: 2013 المجلد: 3 العدد: 5

Article
Effect of cryopreservation on some sperm parameters of infertile patients
تاثير الحفظ بالقري على بعض معايير النطف للمرضى العقيميين

المؤلفون: محمد باقر محمد رشاد --- اسراء مجيد رشيد
الصفحات: 9-15
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الخلاصة

Background: Alt men have the natural desire to have their own biological children. Cryopreservation of male gametes is an important aspect of human fertility preservation. With the advancement in assisted reproductive technology, indication for sperm cryopreservation is expanding. Objectives: To evaluate the effect of cryopreservation by using Sperm Freeze TM kit on some sperm parameters for infertile patients. Materials and methods: Ninety five semen samples were taken from male patients. The seminal fluid analysis was performed according to the standard criteria of WHO (1999). The patients were divided into four groups depending on their sperm concentration and motility: Normozoospermic, oligozoospermic, asthenozoospermic and oligoasthenozoospermic patients. Cryopreservation in liquid nitrogen was done by using Sperm Freeze TM kit for the samples and thawing was performed after three months. Results: In general, the post-thawing study revealed a considerable highly significant (P <0.001) decrease in the sperm parameters which include the means of sperm concentration. sperm motility, progressive motility and normal sperm morphology as compared to that of pre-cryopreservation. Whereas the percentage of immotile sperm was highly significant (p<0.05) increase as compared to pre-cryopreservation in all groups except for oligozoospermic patients in which there was a significant (p<0.05) increase. Conclusions: After semen cryopreservation, all parameters were significantly decreased.


Article
Evaluation of Different Cryopreservation Protocols of the Testis Using 8-Hydroxy 2-Deoxyguanosine as Marker of DNA Damage.
تقييم الانظمة المختلفة لحفظ الخصية بالقري باستخدام 8 هيدروكسي 2 دي اوكسي كوانوسين كمؤشر لضرر الدنا

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الخلاصة

Background: Chemotherapy and radiotherapy can destroy or severely reduce spermatogenesis and thereby jeopardize fertility in the long term. There is still no medical treatment that guarantees fertility preservation after chemo- and radiotherapy. Now with recent improvement of assisted reproductive technologies (ART) and possibility of using testicular spermatozoa or epididymal spermatozoa at in vitro fertilization (IVF) by intracytoplasmic sperm injection (ICSI), cryopreservation of testicular tissue is an option in fertility preservation for males who will lose spermatogenic cells as a result of chemo-and radiotherapy and for males with azoospermia. Objective: This study is an attempt to evaluate oxidative DNA damage in the mice testicular tissue after cryopreservation/thawing cycle when using different types of cryoprotectants as well as to investigate the changes that occur in mice testicular tissue after cryopreservation/thawing cycle as a model for human being. Methods: Fifty mature fertile male mice between 8- 12 weeks old were used in the current study. For each mouse, one testis was evaluated histologically and immunohistochemically (using 8-Hydroxy 2>-Deoxyguanosine as marker of oxidative DNA damage) without cryopreservation (control group). The other testis was evaluated histologically and immunohistochemically (using 8-Hydroxy 2>-Deoxyguanosine as marker of oxidative DNA damage) after six weeks of cryopreservation using different types of cryoprotectants (cryopreserved group). Results: The microscopically observation of slides obtained from cryopreserved testis differs according to the type of cryoprotectant (glycerol, 1,2 propanediol and dimethylsulfoxide), in the dimethylsulfoxide group, tissue sections showed no major differences versus control group, but in the histological sections obtained from tissue cryopreserved by glycerol as cryoprotectant showed moderate morphological and structural changes as compared with the control group. While, the tissue samples subjected to 1,2 propanediol as cryoprotectant displayed the severest morphological and structural changes as compared with the control group. Immunchistochemical results of the present study showed a highly significant (P<0.001) increase in oxidative DNA damage in the cryopreserved testis (46.78%, 63.45% and 32.59% represent cryoprotectant glycerol, 1, 2 propanediol and dimethylsulfoxide, respectively) compared with control group (19.27%) after six weeks of cryopreservation. Conclusion: From the results of the current study, it was concluded that there was alteration in testis histology after cryopreservation/thawing cycle and increase the level of oxidative DNA damage after cryopreservation/ thawing cycle. As well as dimethylsulfoxide cryoprotectant provide good protection for testis histology and DNA.


Article
Effect of cryopreservation on DNA normality of mice epididymal sperms following in vitro preparation with pentoxifylline and Glycyrrhiza glabra
تاثير الحفظ بالقري على طبيعة الحامض النووي للنطف البربخية للفئران بعد التحضير في الزجاج باستخدام البنتوكسيفلين وعرق السوس

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الخلاصة

Background: Many studies conducted on the pentoxifylline (PX) and Glycyrrhiza glabra (G.glabra) as motility I stimulants showed positive effect on sperm preparation in vitro improving the progressive forward movement. However, it was not known the impact of these stimulants on the nature of | genetic material, especially after cryopreservation. Objective: The experiment was designed to find out any harmful effect of a medium containing a mixture of PX and aqueous extract of G.glabra on the sperm DNA before and after cryopreservation. Materials and Methods: Fifty mature fertile male mice 812-weeks old were used. Epididymal sperms were obtained from caudal region and prepared in vitro by direct activation technique using four media namely;PX alone, G.glabra alone, a mixture of PX with G.glabra and Ham's F-12 (as control medium). Certain sperm function characteristics were examined as well as evaluation of DNA integrity | by acridine orange test and comet assay before and after cryopreservation. Results: The results showed a highly significant (P<0,001) improvement in the sperm forward movement, with a highly significant (P0.001) decrease in DNA abnormality using acridine orange test | and comet assay, following in vitro activation by the four media compared to before activation. Following in vitro activation and cryopreservation, the results of epididymal sperm showed a highly significant (P<0.001) decrease in DNA abnormality by using PX medium alone, G.glabra medium alone and the mixture of PX and G.glabra compared to control medium and the results before cryopreservation. Conclusion: According to the results of present study, it has been found no toxic effect of medium containing PX and/or G.glabra on the epididymal sperms DNA after activation in vitro and cryopreservation using acridine orange test and comet assay. The best result obtained when using a medium containing a mixture of PX+ G.glabra together. These data could be applied for preparation and activation of epididymal and testicular sperms obtained from obstructive azoospermic men.


Article
The Role of E-cadherin in the embryo implantation and its site in the endometrium
دور مادة الايكادهرين في عملية انبات الجنين ومكانه في بطانة الرحم

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الخلاصة

Endometrial receptivity is defined as a temporary unique sequence of factors that make the endometrium receptive to the embryonic implantation. Histological evaluation, now considered to add little clinically significant information, should be replaced by functional assessment of endometrial receptivity. A large number of functional molecular mediators have been identified to date, including adhesion molecules. Thus, endometrial biopsy samples can be used to identify molecules associated with uterine receptivity to obtain a better insight into human embryo implantation. Objectives: to investigate E-cadherin expression in the endometrium throughout the menstrual cycle of fertile females Patients, Materials and Methods: Under simple analgesia, fractional endometrial biopsies from anterior, posterior, fundal walls, and the cervix of (32) fertile females(age= 29.91 ± 5.9 year, parity= 3.62.1±) were taken at different days of the menstrual cycle. The immunohistochemical (IHC) stained tissues were analyzed using computerized image analysis for measurement of PI of E-cadherin which is ratio of the number of stained cells to the number of total cells. PI were measured in three regions: stromal, membranous, and glandular epithelium. Rcsiilts: E-cadherin expression in the endometrium is up-regulated in the proliferative phase and down-regulated in the secretory phase of the menstrual cycle. The PI of E-cadherin in the glandular epithelium of the anterior wall of the endometrium was significantly lower than that of posterior and fundal walls (P=0.027). The PI of E-cadherin in the endocervical epithelium was significantly higher than that in the anterior, posterior, and fundal walls of the endometrial cavity (P=0.004). Conclusion: Conclusion: E-cadherin might have a role in embryo implantation


Article
Studying the risk factors for ectopic pregnancy among spontaneous and assisted conception
دراسة عن عوامل الخطورة للحمل خارج الرحم الناتج عن الحمل الطبيعي والحمل المساعد

المؤلفون: فاخر سلمان العاني --- هدى مالك محمد
الصفحات: 38-43
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الخلاصة

ackground: An ectopic pregnancy, or eccyesis, is a complication of pregnancy in which the pregnancy implants outside the uterine cavity. Theoretically, differences between conception via ART and natural conception may affect the risk of ectopic pregnancy. Data on the risk factors for developing ectopic pregnancy after IVF are still inconsistent. There is controversy whether ART increase or decrease the ectopic pregnancy. Objectives: To describe the occurrence or risk factors of ectopic pregnancy following IUI, IVF and spontaneous pregnancy Putlcnts and Methods: The recorded cases of ectopic pregnancy in the period between January 2010 to January 2011 in four teaching hospitals and two private hospitals were surveyed. New cases in the period between July 2011 to July 2012 in two teaching hospitals were also included in this study. The data was collected from medical records and interview. Results: A total of 86676 pregnancies were recorded during the period of the study with, the number of ectopic pregnancy out of total pregnancies in Baghdad was 408 during the period of this study. It was found that the type of conception was significantly associated with infertility (P < 0.001), ovulation induction (P<0.001), dilatation and curettage (P = 0.016), and history of contraception (P =0.002). Conclusion: Although several risk factors for ectopic pregnancy are known, the causes of increasing incidence of ectopic pregnancy remain unknown. The risk factors of ectopic pregnancy following IUI, IVF and spontaneous pregnancy included history of infertility, medication used for ovulation induction, history of contraception, and dilatation curettage as these factors are tightly linked with the types of conception. Increasing knowledge of risk factors for ectopic pregnancy may improve understanding of the causes of infertility.


Article
Relationship between potency of hypo osmotic swelling test and antisperm antibody assay for fertile and infertile men in Thi-Qar governorate
العلاقة بين فعالية فحص كفاءة النطفة البشرية تحت الضغط الازموزي الواطئ مع فحص الضداد المناعية المضادة للنطف للرجال الاسويا والعقيمين في محافظة ذي قار

المؤلفون: باسم خميس كوتي الركابي
الصفحات: 44-48
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الخلاصة

Background; The sperm plasma membrane activity and absence of antisperm antibodies in infertile patients ejaculate significantly associated with fertilization potential of human spermatozoa. The sperm viability detected by hypoosmotic swelling test (HOST) and autoimmune infertility detected by antisperm antibody assay presented diagnostic and prognostic tools for male infertility factors. Objective: This study was devised to compare the results of hypoosmotic swelling test and antisperm antibody assay for normozoospermic men and infertile patients affected by autoimmune infertility Methods: Thirty semen samples were collected by masturbation and prepared by direct layering technique. Hypo-osmotic swelling test was performed by mixing 0.1 ml of semen with 0.9 ml of a 150 mOsmI L NaCI as a hypo-osmotic solution and direct immunobead assay were used to determine the presence of Antisperm Antibodies (ASA) bound on sperm surface. However, sperm concentration, sperm motility, and normal sperm morphology were evaluated according to World Health Organization (WHO) criteria and subjected to HOS test and ASA assay. The sperm prepared and incubated for 30 minute in 5% C02 at 37°C after in vitro sperm preparation. Result*: The results of the present study reported that the percentage of HOS test score in antisperm antibodies positive sperm samples (52.30 ± 5.2) was significantly lower than that noticed in antisperm antibodies negative (70.30 ± 3.4) sperm samples. Conclusion: It was concluded that the use of HOS test as a simple and diagnostic and prognostic tools for identify male infertility. Further studies are suggested to assess the effect of ASA on sperm plasma functional integrity after ICSI and in vitro fertilization and embryo transfers (IVF-ET).


Article
In vitro effects of terpinoid compounds of Euphorbia helioscopia L. extracts on rats epididymal sperm activity rats
تاثير المركبات التربينية لمستخلص نبات Euphorbia helioscopia L في نشاط نطف الجرذان في الزجاج

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الخلاصة

Background: Many plants have medical importance due to the presence an active compounds in plants extraction acting as a drugs for treatment of several diseases in human and animals. Euphrobia helioscopia extract was used to treat diabetes, removal the warts of the skin, stimulate the nervous system and also used against constipation. Objective: Study the effect of terpinoid extract of E.helioscopia on rat epididymal sperm activity and fertility efficacy of male rats. Methods: This study was conducted at Biol. Dept., College of Science/ University of Babylon and Medical College, Mahidol University- Thailand. The study was designed to purify the active compounds of the Terpinoid of Euphorbia helioscopia extract by Nuclear Magnetic Resonance (NMR), and studying their effects on sperm motility and grade activity of the epididymal sperms of the rats. Six main active compounds were isolated from the terpinoid extract of E. helioscopia due to the differences in color, weight and nature by using NMR. The main terpinoid compounds were named A,B,C,D,E and F. Two concentration (0.5 mg/ml and 1 mg/ml) of each active compound of £. helioscopia extract were added to sperm suspension in vitro and incubated for 30 minutes , then sperm motility and grade activity were assessed and the results were compared to those values before adding the active compounds (control). Results: The results showed that 0.5 mg/ml of C and D active terpinoid compounds caused significant (p<0.05) decrease in sperm motility compared to before adding the extract, while the same concentration of B,C,D,E and F active terpinoids compound caused a significant (p<0.05) decrease in grade activity. The addition of 1 mg/ml of terpinoid active compounds (C,D and F) caused a significant (p<0.05) decrease in sperm motility, while the grade activity significantly (p<0.05) decreased by adding B,C,D,E and F active terpinoid compounds compared to control. The sub - compounds (C1,C2,D1,D2,D3 and D4) had a powerful decreasing effect on the sperm motility and grade activity compared to control and other active compounds. Conclusion: It was concluded that terpinoid compounds of E. helioscopia extract have adverse effects on rat epididymal sperm activity.

جدول المحتويات السنة: 2013 المجلد: 3 العدد: 5