Table of content

Jornal of Biotechnology Research Center

مجلة مركز بحوث التقنيات الاحيائية

ISSN: 18151140
Publisher: Al-Nahrain University
Faculty: Presidency of the university or centers
Language: Arabic and English

This journal is Open Access

About

The Biotechnology Research Center (BRC) in a Al-Nahrain University issued in 2007 the first edition of a tightly seasonal scientific journal named as the journal Biotechnology Research Center (JBRC) which got authorization in 2005 and held an impact number ISSN: 1815-1140.The journal accepts scientific researches in Arabic and English.
JBRC’s main interest is Biotechnology researches in the Medical, Molecular, Agriculture and environmental fields which have an important impact on the public and private sectors in Iraq.
JBRC’s structure consists of editing committee (headed by the manger of the BRC and the editor in chief), consulting committee (contains a well- known iraqi scientists in biotechnology) and editing secretary.
All researches are submitted to the JBRC’s regulations which is mainly is that they must be according to the journal directions and instructions , then the researches will be evaluated by three well-known scientists in the field and after that reviewing occurred by the editing committee to ensure and verify all JBRC’s instructions and regulations are taken into consideration .
A special edition of JBCR is issued to cover all researches that presented to the BRC’s scientific conferences which subjected to all regulations and instructions of publishing in JBCR.

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Contact info

brcn2012@yahoo.comيتم الاتصال عبر البريد الالكتروني لمجلة مركز بحوث التقنيات الاحيائية


0096407707766148او للاتصال تلفونيا
بسكرتارية التحرير
م.م. سعاد محمد مجيدب
محمد منير حسين

Table of content: 2014 volume:8 issue:1

Article
Effect of electromagnetic and Ultra Violate rays on production of Cucumis sativus L.
تأثير المجال الكهرومغناطيسي والاشعة فوق البنفسجية في انتاجية ثلاثة هجن من الخيار Cucumis sativus L.

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Three American cucumber (Cucumis sativus L.) hybrids namely Lot Number (EM 285), labeled number 1, Hybrid super Green F1, labeled number 2 and Hybrid Top CAEEN F1, labeled number 3 were studied. Seeds of these hybrids were exposed to four frequencies of electric current (0, 1, 2, 2.5 ) amber(Am.) for 5 minutes and four periods of exposure for ultra-violate rays (UV) at 224 nanometer (nm) for (0,30,60 and 90) second. The treated seeds were sown on 2010 in open field that prepared and fertilized. The effect of electronic current Am and UV on the production of these hybrids was studied. Flowers number/ plant were recorded after 35 days of sowing; length, radius and weight of fruit were recorded after 46 days of sowing. Results showed significant differences between the hybrids in the studied parameters. Hybrid 3 showed a significant increase compared with hybrids 1 and 2 since it gave the highest number of flowers (19.97 flowers. Plant-1), tallest fruit length and radius of fruit (14.09 and 7.93 cm.fruit-1 respectively) and highest fruit weight 46.97 gm.fruit-1. While hybrid 2 gave 16.72 flower.plant-1, 10.35 cm.fruit-1, 5.72.fruit-1 cm (length and radius of fruit respectively ) and 30.2 g.fruit-1.The results also indicated a significant interaction between electric current and UV in all studied parameters. The 2 Am frequency and 60 sec of UV exposure gave highest flowers number (24.09 flower. plant-1) with an increase 85.16% compared with 2.5 Am frequency and 90 sec. of UV exposure. The results also showed a significant interaction between hybrids, electric current and UV. Hybrid 3 treated with 2 Am and 60 sec exposure gave highest fruit weight 86.33gm.fruit-1 with a significant increase for all tri- interactions.


Article
Cytotoxic effects of Ammi visnaga volatile oil on some cancer cell lines
التأثير السمي للزيوت الطيارة لعشبة الخلة البلدي على بعض خطوط الخلايا السرطانية

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Ammi visnaga a herbal plant is rich with important active constituents that make the plant to be described in traditional medicine and among them is the volatile oil. The oil extracted by distillation and then study it's cytotoxic effect on two cell line: The human pelvic rhanbdomyosarcoma (RD) and The mouse cell line( L20B) which expresses the genes for human cellular receptor for Polio viruses. Results showed that there were potent toxic effects on both cell lines RD&L20B specially at the concentration (100,50 and 25)µl/ml of the essential oil ,then decreased as the oil concentrations decreased.


Article
Evaluation of cellular immune response provoked by experimental infection with Cryptosporidium spp. in mice
تقييم الأستجابة المناعية الخلوية الناتجة عن الخمج التجريبي بطفيلي الابواغ الخبيئة في الفئران

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Experimental infection was induced in immunosupreesed and non-immunosupressed mice using inoculation of two different Cryptosporidium spp. isolates: faecal origin and river water origin; then the infectivity rate and cellular immunological status were examined in these groups of mice. The results indicated that these two isolates induced experimental infection in immunosuppressed and non-immunosuppressed mice with no significant differences in infectivity rate between groups. Immunosuppressed mice infected with feacal or water origin oocyst showed low and significant values of transformed lymphocytes 8.68% ± 0.65%, 9.18% ± 1.09 respectively and phagocytic index 10.1% ± 1.66 and 11.2% ± 0.19 respectively. It concludes that lymphocyte transformation and phagocytosis index are good indicators to evaluate the cellular immune response in infected experimental mice with Cryptosporidium.


Article
Effect of nutrient solution and foliar fertilizers on the characteristics of growth and yield of potato plant Solanum tuberosum L. by sand culture systems
تأثير المحلول المغذي والأسمدة الورقية في صفات النمو الخضري والحاصل لنبات البطاطا Solanum tuberosum L. في الزراعة الرملية

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Abstract

This study was carried out in green houses at the Twaitha site of Agricultural research directorate, Ministry of Science and Technology during 10 December 2010 to 20 February 2011. The study included effect of combination to fertilizer nutrient solution and foliar application on plants potato in the developing sand culture system Randomized Complete Block Design RCBD were adapted this study 8 treatments of fertilizer. F0 without fertilizer, F1nutrient solution, F2 spray megafol activated amino, F3 spray magnum urea & phosphate, F4 spray agroleaf composite balanced, F1F2 nutrient solution + spraying megafol, F1F3 nutrient solution +spraying magnum and F1F4 nutrient solution + spraying agroleaf . L.S.D. were used to compare the means at 5% level of significances: - The results showed superiority of all treatments fertilizer (nutrient solution with the fertilizer) to the attributes under study compared with control treatment F0, treatment of F1F4 significantly increased number of stem, high of plant cm, leaf area Dcm2 and dry weight of shoots of sand culture reached 4.33 stem/plant ,61.67 cm ,97.35 Dcm2 , 30.40 g Compared with the control treatment. And treatment F1F4 was significantly increased the yield of plant reached 672.00g and no significantly difference with F1F3, F1F2 treatments, while the ratio decreased in the F0 treatment reached 229.00g.


Article
Evaluation of some growth promoting bacterial strains exist on Eggplant root Solanumm elongena L. against Rhizoctonia solani
تقييم السلالات البكتيرية المحفزة لنمو النبات ضد الفطر Rhizoctonia solani على نبات الباذنجان Solanumm elongena L.

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Abstract

In this study, six plant growth promoting bacterial strains were tested against eggplant root rot disease caused by Rhizoctonia solani. The bacterial strains were evaluated for their ability to promote growth and control R. solani in eggplant under greenhouse conditions. The results of antagonistic activity of the bacterial strains against R. solani showed that the tested strains controlled the radial growth of R. solani ranging from 24.66 to 40.33 mm, of these, Bacillus subtilis was the most promising strains which recorded 24.66 mm. Results of the treatment eggplant seeds with the bacterial suspension of the six strains showed that all tested strains significantly increased the percentage of seeds germination as compared to control treatment, B. subtilis strain was the best which recorded 92.16% as compared to 69.56% for control treatment. The greenhouse experiment revealed that the plants treated with B. subtilis recorded maximum (Shoot length, root length, fresh and dry weight of plant, rate of fruits weight, plant productivity). All these parameters were increased by 93.83 cm, 26.50 cm, 589.30 g/ plant, 163.03 g/ plant, 101.63 g, 1180 g/ plant respectively, also the results showed B. subtilis significantly decreased disease incidence and severity of eggplant infected by R. solani which recorded 34.06, 0.23 respectively as compared to both positive without pathogen and negative with pathogen control treatment (4.4 %, 77.33), (0.07 %, 0.71) respectively.


Article
Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on testis histological traits of roosters subjected to oxidative stress induced by hydrogen peroxide
تأثير اضافة مسحوق بذور الجرجير Eruca sativa الى العليقة في الصفات النسجية للخصية للديكة المعرضة للإجهاد التأكسدي المستحث ببيروكسيد الهايدروجين

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Abstract

This study was conducted to evaluate the effect of adding rocket salad seeds powder to the diet on testis histological traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed on 5 treatments with 3 replicates each treatments were as follows: T1: males were fed control diet and normal water, T2, T3, and T4: males were fed control diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide 0.5%/ litter of water, 0.5 ml hydrogen peroxide 0.5%/ litter of water, and 1 ml hydrogen peroxide 0.5%/ litter of water, respectively. The T5: males were fed control diet and drank water supplemented with 1 ml hydrogen peroxide 0.5%/ litter of water. Males were treated with hydrogen peroxide 6% and rocket salad for 12 weeks starting from 59 week of ages. Results revealed that water supplementation with hydrogen peroxide without adding rocket salad powder to the diet (T5) resulted in significant deterioration as regards testis weight, measurements of seminiferous tubules, and volume density and relative weight of active components of seminiferous tubules and interstitial tissue as compared with T1 group. However, supplementing the diet of roosters subjected to oxidative stress induced by hydrogen peroxide with rocket salad seeds powder (T2, T3, and T4) resulted in significant improvement concerning histological traits involved in this experiment in comparison with T5 group. In conclusion adding rocket salad powder to the ration of roosters could limit to a considerable extent the damages on histology of the testis that originate from oxidative stress. Therefore, rocket salad powder could be used as one of active tools for improving reproductive performance of roosters.


Article
Detection of Perforin in suspension of leukemia lymphocyte culture
الكشف عن البيرفورين (الثاقب) في عالق المزرعة ابيضاض الدم اللمفاوية

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This work aimed to study the concentrations and hemolytic assay of perforin in serum and culture suspension of lymphocyte for acute lymphocyte leukemia ALL; chronic lymphocyte leukemia CLL, and control. To achieve this goal, blood samples were collected from 20 leukemic cases 10 ALL and 10 CLL, and isolation of lymphocyte done then proliferation of leukemia lymphocyte in culture for the detection of perforin concentration by ELISA kit and hemolytic activity of perforin in suspension lymphocyte culture and serum were impacted. The results, showed significant increase in the level of concentrations of perforin in both CLL and ALL as compared with the control, however, showed significant lower in level of hemolytic activity of perforin in these groups as compared to the control. The conclusion is that expression of perforin is strongly associated with leukemia patient in Iraqi population.


Article
Study the Effect of Temperature on the Performance of Hollow Fiber Membrane Bioreactor in Wastewater Treatment
دراسة تأثير درجات الحرارة في اداء مفاعلات الاغشية الحيوية لمعالجة مياه المجاري

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A membrane bioreactor (MBR) is one of the modifications to the conventional activated sludge process, since it is the combination of a membrane module and a bioreactor. In the present study, 100 liters lab-scale aerobic MBR was seeded with 1.5 Liter activated sludge and municipal wastewater from AL-Rustumiya municipal wastewater treatment station, two hollow fibers sample (MI,MII) manufactured in the University of Technology/ Chemical Engineering Department, were used as biomembranes. Trans membrane pressure TMP was studied and it was found that the optimum value of TMP was 10 cm Hg vacuum which gave optimum effluent flux 400 ml/hr for MI and 350 ml/hr for MII. The experimental work involves the effect of temperature 25, 35, 45°C on the performance of the MBR fibers sample (MI, MII) and its effect on biomass growth and removal efficiency of the COD, BOD. Both samples show good performance in 25°C.


Article
The effect of Hydrogen Peroxide on the viability of Beauveria bassiana and their ability for radial growth on Chitin Agar
تأثير بيروكسيد الهايدروجين في حيوية الفطر Beauveria bassiana (Bals.) Vuill. وقابليته على النمو القطري في الوسط الزرعي اكار كايتين

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This study aimed to evaluate the effects of five concentrations of hydrogen peroxide (50, 100,200,300,400) ppm on the viability of Beauveria bassiana spores after 1,2,3,4 days of exposure at 28 ± 1C° and on the radial growth ability of the fungus on chitin agar medium after 11 days of incubation at 26 ± 1C°.This fungus was also used to control the Date palm dubas Ommatissus lybicus. The results showed that the viability of B. bassiana spores of control (distill water with fungal spores) of a rate ranged between 157 – 257 colony/ petri dish on potato dextrose agar and the rate 257 colony/petri dish was higher than those of other treatments, while the rate 91.7 colony/petri dish at 50ppm concentration of hydrogen peroxide was the best p= 0.05 in comparison with the viability rate of other treatments in which the spores were exposed to highest concentrations of hydrogen peroxide. It was also seen that spores lost their viability after exposure to 400 ppm of hydrogen peroxide. On the other hand, the results showed that the rate of radial growth 3.46cm in control group after 4 days of incubation in comparison with this in the other group was higher. The fungus showed lowest rate of radial growth 0.43cm after one day of incubation p= 0.05, while the one day exposure of spores to each of 50,100 ppm of hydrogen peroxide showed 3.47,3.5cm respectively which were significantly higher p= 0.05 in comparison with other concentrations, improving which the higher concentration the lower activity of fungal degrade chitin.


Article
Curing of Escherichia coli Antibiotic Resistance Plasmids by Aspirin
تحييد بلازميدات مقاومة مضادات الحيوية من بكترياايشريشيا القولون بواسطة الاسبرين

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Abstract

An isolate of Escherichia coli (E. coli) was isolated from urine sample due to person infected with urinary tract infection (UTI).The isolate was resistant to the following antibiotics: Ampicillin, Cotrimoxazole, Chloramphenicol and Tetracycline. Agarose gel electrophoresis of its plasmids content has revealed the presence of single large plasmid and two small plasmids bands. The large plasmid was conjugative and contained the resistance genes for four antibiotics. In vitro curing of this plasmid was achieved by treatment with salicylic acid (aspirin) with 150,200,250 and 300 µg/ml as indicated by the elimination of resistance, also by absence of large plasmid band following agarose gel electrophoresis. In vivo curing was conducted using New Zealand rabbits. UTI was induced by bacterial inoculation via urethral catheterization. The E. coli from urine samples of the rabbits, the count of which was proportional to the type of treatment. Minimum number of colonies was associated with group treated with metheprim was aspirin 300mg/kg daily dosage. This result may indicate that the side effect of metheprim was in its maximum with aspirin. Survivor bacteria may indicate incomplete exposure to the drug.


Article
Exploitation of solar energy as an alternative energy for milk sterilization from microbes
استغلال الطاقة الشمسية كطاقة بديلة في تعقيم الحليب من المكروبات

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Abstract

In this research the milk had been pasteurized by solar energy. It is clean energy and friendly to environment as well as available in Iraq. Circle option dish reflector was used with diameter1.5 m and focal length 80 cm, reflectivity gave at 80% and heat temperature about 200-350c. Milk container location was in optical focus of optical dish reflector. The milk had been heated at different times 10,20,30 minutes and we showed that after implanting samples tested to three genus of bacteria-contaminated milk (Staphylococcus, Klebsiella, Enterrobecter), the bacteria did not appear in 30 min heating.


Article
Cytotoxicity of myriocin against axenic culture of Leishmania mexicana
التحري عن سمية الميريوسين في المزروع المختبري لطفيلي Leishmania Mexicana

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Serine Palmitoyltransferase SPT is the key enzyme in the de novo sphingolipids biosynthesis pathway in eukaryotes, including the intracellular parasite Leishmania. Previous studies showed that this enzyme SPT is expressed only in divided promastigote forms and it is non-essential in the amastigotes form of Leishmania major, which is known as the old world leishmaniasis. In this study we have studied the viability of new world lesihamniasis, Leishmania mexicana. Cytotoxicity test used to determine the effect of the SPT inhibitor myriocin which did not significantly affect the viability of the two forms of the in vitro cultures of the parasite p<0.05, procyclic promastigotes and amastigotes, in which cell viability for miltefosine treatment 100, 50, 25, 12.5, 6.25µM against procyclic promastigotes form was 133.7, 115.8, 103.7, 94.98, 94.78 respectively for 24 hours incubation, while for amastigotes, cell viability for miltefosine treatment was 114.77, 114.34, 104.21, 132.95, 102.74 respectively for the 24 hours incubation and was 81.46, 81.25, 71.40, 68.38, 66.51 respectively for the 48 hours incubation and was 78.99, 90.22, 85.59, 95.18, 84.43 respectively for the 72 hours incubation. While in the old world leishmaniasis, Leishmania major, it has been found that ceramide production is absent and non-essential for the proliferation of intra-amastigotes.


Article
Study the effect of some acids on the viability of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae and their growth ability on chitin agar
تأثير بعض الاحماض في حيوية الفطرين Beauveria bassiana (Bals.) Vuill. و Metarhizium anisopliae. وقابليتهما في النمو على وسط الكايتين

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This study aimed to evaluate the effects of three acids; Citric, Oxalic and Boric acids 0.05% on the viability of Beauveria bassiana and Metarhizium anisopliae spores after 1,2, 3 days of exposure 27±1 C° and the growth on chitin agar was obtained after 5 days at 26 ± 1C°.The results showed that the rate of viability of B. bassiana and M. anisopliae with boric acid was 312.3,209,150.3 colony/ml and 318,294.7,157.5 colony/ml respectively during three period time of in comparison with control and spore suspensions with the other acids. Spores of both fungi B. bassiana and M. anisopliae showed maximum rate of viability in the suspension supplied with citric acid which were 266 and 293.6 colony/ml, respectively after 1day of exposures to this acid in comparison with viability of control and other periods. Spores viability was lost with oxalic acid. On the other hand, control recorded highest rate of radial growth in the control was 1.92, 4.4 cm on the medium chitin agar after 3 days of incubation in comparison with other treatments. Both fungi showed best rate of radial growth 3.12, 1.88cm after 1and 3 days of exposure to citric acid, respectively in comparison with other treatments. There were no such growths on chitin agar after exposure the oxalic acid.


Article
Analysis of common mutation for GALT gene in newborns with galacatosemia Nineveh governorate
تحديد الطفرات الوراثية التي تصيب ألجين GALT لدى الأطفال المصابين الكلاكتوسيميا في محافظة نينوى

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Iraq contains many diseases that have never been counted or examined, including diseases related to food, which has deteriorated in recent years, and has rapid and direct impact especially on the children category, one of these diseases is galactosemia. Classical galactosemia, deficiency of galac tose-1-phosphate uridyltransferase GALT, is characterized by acute symptoms of hepatomegaly, jaundice, sepsis, cataract, vomiting, and diarrhea and growth retardation. Our previous molecular study showed that the most common mutation of the GALT gene is a missense mutation of Q188R (replacement of glutamine-188 by arginine in exon 6 and N314D mutation replacement of aspargen-314 by aspartic acid) in exon 10. The aim of this study was to determine the possibility of diagnosing galacatosemia, and to search for galactosemia mutation Q188R and N314D in Iraqi population. Blood samples were collected from babies admitted to the children's hospitals in Mosul City depending on the clinical symptoms of disease and then serum was taken. Measuring the Galactose-1-Phosphate uridylytransferase GALT enzyme activity and galactose -1- phosphate in serum by ELISA technique was done. DNA samples were analyzed by the polymerase chain reaction followed by digestion with restriction endonuclease HpaΙΙ and AvaΙΙ for Q188R and N314D mutation. The results showed a significant decrease in the level of the GALT enzyme in children with galactosemia 21.7 ± 0.45 and among non-diagnosed children 79.93 ± 1.44 compared with control group 160.33 ± 0.93 as well as a significant decrease in the level of the enzyme among mothers 20.5 ± 1.92 was observed. Gal-1-P level was significantly higher in the cases than that of the control group, while that of the not diagnosed children and mothers groups showed inconsistent difference. Also the result showed absence allele frequency for Q188R mutation and present allele frequency for N314D mutation in Iraqi population. In conclusions It is possible to depend on measurement of Galactose-1-Phosphate as indicator in the diagnosis of Galactosemia in newborn, the main mutation in GALT gene causes galactosemia is N314D in Iraqi population.


Article
Influence Soaking Gibberellin acid on seed germination for Citrus limetta and Citrus limonum and their seedlings growth
تأثير النقع بالجبريلين في إنبات بذور الليمون الحلو Citrus limetta والليمون الحامض Citrus limonum وفي نمو الشتلات الناتجة

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Study was undertaken to test the effects of soaking seeds Citrus limetta and Citurs limonum in Gibberellic acid (GA3) solutions 0, 100, 500, 1000 mg / L for 24 hours prior on the rate of germination, some morphological characteristics for seedlings also were investigated in this study. Factorial experiment was carried out 4×2×5 in a randomized complete block design with five replicates for each type of seed during the period of March to September 2011. The results showed significant differences in most traits, as it reached the highest percentage of germination for the treatment with 500 mg/l for both types of seeds, while the highest dry and fresh weights of shoot and root, main root length, highest shoot, leaves number and leaf area index were in 1000 mg /l treatment. No significant effect of GA3 treatment on the amount of A, B and total chlorophyll in the seedlings leaves. The best treatment was soaking seed in 1000gm/l Gibberellic acid. The information documented the stimulating effects of seedling be used to increase the ratio of germination. Also gibberellins stimulated effects continues for some seedlings morphological characteristics and progressively with gibberellins seed treatments influence vary according to genotypes of citrus seeds, that can increase concentrations which used in soaking seeds solutions.


Article
Using Random Amplified Polymorphic DNA (RAPD) analysis to investigation of genetic diversity, and relationships among a set of clinical Aspergillus fumigatus isolates
استخدام مؤشرات ال RAPD في تشخيص التغايرات الوراثية والعلاقة بين عزلات سريرية من الرشاشيات الدخناء

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This study is an attempt to determine the genetic diversity and relationships among fourteen local isolate isolated from patients with Aspergillosis (Aspergillus fumigatus) by using the Random Amplified Polymorphic DNA (RAPD) technique. Twelve universal primers used in this study produced 94 bands across fourteen isolates. Of these bands, 67 bands or 71.2% were polymorphic. The size of the amplified bands ranged between 100-2000 bp. The genetic polymorphism value of each primer was determined and ranged between 33-100%. In terms of unique banding patterns, determine the finger print for six isolates the most characteristic banding pattern was for the (AFU1, AFU2, AFU3, AFU4, AFU8 and AFU14) with primer (OP F-16 , OP I-06, OP F-16, OP X-01, OP X-01and OP A-06). Genetic distances ranged from 0.12419 to 0.64404 among A. fumigatus isolates. Cluster analyses were performed to construct a dendrogram among studied A. fumigatus isolates. The cluster analysis places most of the A.fumigatus isolates isolated from patient come from yhe same area into a close relation (subcluster) showing a high level of genetic relatedness and were distinct from isolates from another area (the other subcluster). Interestingly, a number of isolates originating from the same sources did form well defined groups, indicating association between the RAPD patterns and the geographic origin of the isolates. The information generated from this study can be used in the future for controlling of Aspergillosis programs.


Article
Plymorphism of CYP17 for Polycystic Ovarian Syndrome in Women of Salah Al-Din Provence/ Iraq
ارتباط تعدد اشكال الجينCYP17 مع متلازمة تكيس المبايض PCOS لدى نساء محافظة صلاح الدين/ العراق

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CYP17 gene is the key in the metabolic pathway of sexual steroids, it codes for two enzymes 17–alpha hydroxylase and 17, 20 lyase who play an important role in the production of sex steroid hormones. The current study included study of the relationship of this gene with PCOS women in reproductive age. Blood samples were collected from two groups of women: first group consists of 98 women infected with polycystic ovaries syndrome, the second consists of 25 healthy women to detect the presence of mutation within the gene, then calculate allele frequency of mutant and wild allele for this gene, then repeat genotypes in infected women and compared with healthy once, were calculated body mass index BMI of two groups and measure the level of sex hormones (LH, FSH, Prolactin, Testosterone) in both groups, were the results showed significant differences between patients and healthy women in BMI and the level of hormones (LH, Prolactine) while there is no significant differences in the (FSH, Testosterone) hormone. Three pattern of genotype were obtained: TT, homozygous wild, (TC) much lower than the frequency in the healthy 0.82 while the frequency of allele C among patients 0.44, which is more than double than in healthy 0.18. The current results showed absence CC genotype in healthy women and appeared only in women with PCOS. This monitor the presence of link between this gene and PCOS as may be considered the mutation -34 (T C) in the promoter of gene CYP17 marker of the disease.


Article
n vitro micro propagation of Spilanthes acmella (L.) Murr
الإكثار الخضري الدقيق لنبات .Spilanthes acmella (L.) Murr خارج الجسم الحي

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This study was aimed to In vitro propagation of Spilanthes acmella L. Murr. It is a medicinal plant not cultivated in Iraq. Seeds were sterilized and cultured on MS medium. Indole acetic acid IAA, Benzyladenin BA growth regulators’ were used at the initiation stage. The combination between IAA and BA was used in multiplication stage. Indole butyric acid IBA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. A node exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1mg/l. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding BA at 0.5, 1.0 or 1.5 and IAA at 0.1 mg/l. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 10, 84, 10.00 respectively. Adding 0.1, 0.5, 1.0 mg/l IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots respectively with mean length of 3.25, 3.80, 3.80 cm respectively. Results of acclimatization stage showed that addition of 1:1 Patmos and loamy soil gave the highest rate of survival 100% after 4 weeks of acclimatization. This study showed the ability of in vitro propagation of Spilanthes acmella (L.) Murr


Article
Saccharomyces boulardii as effective probiotic against Shiegella flexneri in mice
دراسة القدرة العلاجية التعزيزية لخميرة Saccharomyces boulardii ضد بكتريا Shiegella flexneri

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This study was designed to evaluate the ability of Saccharomyce buolardi as effective probiotic against Shiegella flexneri. Mice treated with S. boulardii and infected with Sh. flexneri, then serum levels of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) of treated mice were measured and histological sections were made from liver to evaluate protective effect. Results showed that mice treated with S. boulardii exhibited no significant p≤0.05 differences in serum level of AST and ALT 131,67 respectively U/L in comparison with their levels in serum of control group 113.2, 72.86 U/L. Mice infected with Sh. flexneri showed a significant increase in serum level of AST and ALT 198, 101 U/L in comparison with their levels 113,72 U/L in control group. Mice treated with S. boulardii and infected with Sh. flexneri showed a significant decrease in serum level of AST and ALT in comparison with their levels in mice infected with Sh. flexneri 80.13,78.26 U/L vs. 198 and 101 U/L respectively. Histopathological study showed that infection with Sh. flexneri caused a necrosis, degenerative changes and inflammatory cells infiltration as compared with control, while treatment with S. boulardii prevented the histopathological effect of Sh. flexneri.


Article
Early detection of Breast Cancer by Tumor Marker CA15.3, CA27.29 and relationship with P53 and Vitamin D
الكشف المبكر لسرطان الثدي بواسطة الدالات الورمية 29.CA15.3, CA27 وعلاقتها مع بروتين P53 وفيتامين D

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Abstract

Breast cancer is the second most frequent cancer of women. Tumor markers are substances that can be found in the body when cancer is present. The classic tumor marker is a protein that can be found in the blood in increased levels when the cancer is present.The present study have been investigated the levels of cancer antigen CA15.3, CA27.29, P53 and Vitamin D in women with breast cancer, aged were 30-75 years old, they divided into four groups, each group composed of 50 women included breast cancer patients aged 30-59 year, breast cancer patients aged 60-75 years, milking nodules groups 30-60 year and control groups. This study showed significant P<0.05 increasing in the level of CA15.3, CA27.29 and P53 concentration in milking nodules and both breast cancer groups, also showed significant P<0.05 decreasing in vitamin D concentration in milking group and breast cancer group and significant difference in serum Ca concentration.


Article
Identification of Archaea species from hot spring water in Hammam AL-Allel at Mosul Governorate
تشخيص بعض انواع الـ Archaeaمن مياه الينابيع الحارة في حمام العليل بمدينة الموصل بالاعتماد على الصفات المظهرية والفحوصات الايضية و تفاعل البلمرة المتسلسل PCR

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Abstract

Isolate, Identify of some species belonging to Archaea from water of hot and sulphid springs in Hammam Al-Allel City/ Nenavah Governorate. Seventy five samples were collected from hot springs during 2012. The Archaea species were isolated using ordinary and selective media then identified according to morphological characters such as shape, colour, consistency of colonies,The isolated Archaeal species showed optimum growth at temperatures between 37-40 C°, while Halobacterium spp. grown at 30-50 C°, and Sulfolobus spp. between 35-70 C°. and when grown in different levels of pH. The results showed the ability of Halobacterium spp. to grow at pH between 4-7 and optimum growth at 3.5-4.5, while Sulfolobus spp.was shown good growth at 3-7 and optimum growth at 3-4, when grown in different concentrations of sodium chloride.The results showed that Halobacterium spp.were grow well in concentrations between 4.5-5.5% while Sulfolobus spp.were grown in concentrations between 2-3%. PCR technique based on amplification of genomic DNA using Specific primers for Archaeal species, was used to confirm the identification of Archaeal and the result indicated one distinct band with molecular size of 930-950 bp in comparison with DNA ladder after electrophoresis on agaros gel.The result of identification showed three species of Archaea in these studied springs, Sulfolobus solfataricus, Sulfolobus acidocaldarius, Halobacterium salinarum.


Article
Response of four medical plants of Euphorbia species in callus initiation in vitro
استجابة اربعة نباتات طبية من جنس Euphorbiaلاستحثاث الكالس في خارج الجسم الحي

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Abstract

Callus cultures were initiated for four Euphorbia species. Nodule explants cultured on Murashige and Skoog (MS) medium, supplemented with different concentrations 0,0.5,1,1.5,2 mg/l of the auxin 2,4-Dichlorophenoxy acetic acid 2,4-D. Half of the cultures were incubated for16 hrs/day photoperiod, while the other half was incubated under complete darkness. The incubation temperature was 25±1ºC. Observations on number of nodule explants initiated callus were recorded at 2,4,6,8 weeks of culture. For callus maintenance, 50mg of callus produced were re-cultured on MS medium supplemented with different concentrations of 2,4-D 0,0.4,0.8,1.2,1.6 mg/l. Callus fresh and dry weights were recorded after 4 weeks. Results showed that nodule explants of Epeplus and Ehirta incubated under light conditions achieved the highest response to callus initiation 75-100% compared with the other species under experimental conditions. E.Helioscopia incubated under light conditions achieved the lowest response for callus initiation 25-75%. Results also showed significant differences between Euphorbia species in fresh and dry callus weights, E. Ehirta produced the highest fresh and dry weight of callus reaching 1.410 and 0.046 mg respectively. The amount of fresh and dry weight of callus produced under dark conditions was significantly higher than that produced under light conditions.


Article
Ability of the fungus Aureobasidium pullulans to produce polysaccharide (pullulan) with presence of different Carbon and Nitrogen sources
قابلية الفطرAureobasidium pullulansعلى انتاج السكرالمتعدد(البوليولان) بوجود مصادر كاربونية ونايتروجينية مختلفة

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Abstract

The effect of incubation period, different carbon and nitrogen sources on the production of the extracellular polysaccharide pullulan by Aureobasidium pullulansNRRL58560 was examined. The results showed that the maximum production of pullulan was obtained 13.76 after 96 hours of incubation. Sucrose as a carbon source gave the highest production of pullulan 31.92 g/l while the highest biomass was obtained when starch was used as a carbon source 16.30 g/l. The amino acid glycine as a nitrogen source gave the highest production of pullulan 34.61 g/l and the highest production of biomass 14.49 g/l was obtained when ammonium chloride was used.


Article
Influence of hot alcohol extraction of Celery fruits on renal stones dissolution in vitro.
تاثير المستخلص الكحولي الحار لثمار نبات الكرفس على تفتيت بعض انواع من حصى الكلى خارج الجسم الحي

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Abstract

This study was carried out to investigate the effect of hot alcohol extraction activity of Apium graveolens fruits on the common Renal stones dissolution (Ca-OX ,Uric acid) kinds and volatile oils concentrations on Apium graveolens via HPLC were estimated. Fourteen compounds which were (α-pinene, Cineole, P- cymene, Terpinene, α- phellandrene, β-pinene, Geraneole, α-terpinole, Linalool, Menthone, Limonene, Myrcene, Camphen, Rutin) were measured. In regard to concentration, α -pinene has the highest concentration since it reaches 19.2 µg/ml, while the lower concentration was achieved with Camphen which was 6.3 µg/ml. Results of Ca-Ox renal stones treated with extract of Apium graveolens showed significant decrease in stone weight since highest weight decrease percentage, using the hot alcoholic extract reaches 17.02 % at 5 % concentration and in comparison with Rowantinex drug, the results showed a high similitude between the hot alcoholic extract at 5% concentration and Rowantinex drug in respect of percentage of renal stones dissolution, so hot alcohol extraction of apium gravedens in 5% concentration was the best and active in dissolution Ca-OX at first degree and uric acid at second degree.


Article
Characterization of Saprolegnia spp. isolates from infected eggs, fry and adults of common carp Cyprinus carpio L. based on molecular data in Al-Manahel and Al-Wahda fish hatcheries, in middle of Iraq
توصيف انواع مختلفة من الفطرSaprolegnia من بيوض وافراخ وبالغات اسماك الكارب الاعتيادي Cyprinus carpio L . بالاعتماد على البيانات الجزيئية في مفقسي اسماك المناهل والوحدة وسط العراق

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Abstract

The aim of this study was to identify Saprolegnia spp. isolated from inffected common carp Cyprinus carpio L. eggs and fry in two fish hatcheries (Al-Manahel and Al-Wahda) in the middle part of Iraq, during the period from March-June 2009. It was evident from molecular diagnosis (PCR Technique) that isolates of the genus Saprolegnia, shared one feature (production of secondary zoospores with long hooked hairs). These isolates were divided into four groups based on the findings of molecular diagnostics PCR. Isolates of Saprolegnia spp. were characterized genetically and physiologically. The majority (25 from45) of the isolates in both hatcheries were almost genetically identical as assessed by RAPD-PCR. The remaining isolates belonged to three different groups.

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