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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2008 المجلد: 7 العدد: 2

Article
GENETICAL AND MOLECULAR ANALYSIS OF FAMILIAL HYPERCHOLESTEREMIA DISEASE
التحليل الوراثي والجزيئي لمرض فرط الكوليسترول العائلي

المؤلفون: عبدالحسين مويت الفيصل
الصفحات: 133-146
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الخلاصة

The mendelian autosomal dominant diseases are a group of diseases which raised by a dominant mutated allele of a single gene. Most of these dominant genes are heterozygous where the homozygous are uncommon . All of these genes are located on autosomal chromosomes and autosomal dominant disorders affect both sexes and can often be traced through many generations of a family ( Vertical Transmitting) . The heterozygous affected parents will transmit the gene for the disease to half their offspring, whether male or female. Estimation of risk is therefore apparently simple by using family pedigrees but in practice several factors such as expressivity and penetrance may cause difficulties in families counseling Familial Hypercholesteremia – FH- is represented as an example for autosomal dominant disorders .This disease raised from deleted or damaged low density lipoproteins -LDL-receptor resulted in defective clearance and hence elevated levels of LDL including cholesterol which can cause different types of coronary and heart diseases and increase severity with early death. The FH disease is due to a different types of genetic defects ranging from a single mutation to deletion in the LDL- receptor gene which is located on the short arm of chromosome 19 (p13.1-13.3).

الكلمات الدلالية

Autosomal --- FH --- LDL


Article
THE EFFECT OF SODIUM FLUORIDE ON HISTOLOGICAL CHANGES
تأثير فلوريد الصوديوم NaF في التغييرات النسجية للمبيضين وقرني الرحم في الفئران

المؤلفون: حسام جاسم حسين بنانة
الصفحات: 147-156
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الخلاصة

The present study was conducted to identify the effect of oral administration of sodium fluoride (NaF) on histological changes of ovarian and uterine horns in mice. Sixty four mature female Swiss albino mice, Balb/C were divided into four major groups including control (C) group and treatment groups (T1 , T2 and T3) according to NaF dose as following 0 ppm, 200 ppm, 400 ppm and 600 ppm , respectively. Each major group was subdivided into two minor groups according to the period of administration in 10 and 15 weeks. Therefore, each minor group contains eight females. Histological changes were assessed includes ovarian diameter, number and diameter of growing ovarian follicle (GOF), Graafian follicles (GF) and corpus luteum (CL), diameter of uterine horn (UH), thickness of epithelial cell layer (ECL) lining the UH and diameter of uterine glands (UG). The results of ovarian histological changes showed a highly significant (P<0.01) reduction in the ovarian diameter, number and diameter of GOF, GF and CL for all treated groups T1, T2 and T3 as compared to control for both treatment periods (10 and 15 weeks). Within same treatment periods, histological examination of uterine horn observed highly significant (P<0.01) reduction in the diameter of UH, thickness of ECL and UG for all treated groups as compared to control. It was concluded that the administration of NaF to female mice have harmful effects on histological changes of ovaries and uterine horns; and consequently may have a negative effects on female fertility and fecundity.

الكلمات الدلالية

Sodium fluoride --- female mice --- fertility.


Article
GROWTH OF STAPHYLOCOCCUS AUREUS AND PRODUCTION OF HEMOLYSIN IN DIFFERENT PLANT AND ANIMAL WASTES MEDIA
نمو بكتريا Staphylococcus aureusوانتاج حال الدم (hemolysin) في أوساط من مخلفات نباتية وحيوانية مختلفة

المؤلفون: شذى سلمان حسن
الصفحات: 157-167
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الخلاصة

Four different media prepared from plant and animal wastes included rice bran, sunflower meal, potato peels and chicken bones, further more Brain Heart Infusion was used as commercial medium. The media was prepared as solid media (with agar and blood) and liquid media in two concentrations 5% and 10%.The media were cultured with S. aureus, growth and hemolysin were detected in solid media and quantitatively estimated in liquid media. S. aureus grew and produced hemolysin in all these media. The highest growth was observed in 5% and 10% rice bran. Hemolysin activity in 5% rice bran was more than in other media, followed by sunflower meal, ckicken bones and potato peels respectively. The bacterial growth in 10% concentration was more than in 5% in most media, while hemolysin activity was nearly equal in the two concentrations, except in rice bran in which hemolysin in 10% was more than in 5%. These results indicated that the plant and animal wastes used are suitable for bacterial growth and hemolysin production, rice bran is the best one for this purpose.

الكلمات الدلالية

Staphelococcus aureus --- growth --- hemolysin --- waste media


Article
RESPONSE OF DIATOM NITZSCHIA PALEA CULTURE TO DIFFERENT CONCENTRATIONS OF NITROGEN, PHOSPHORUS AND SILICON
استجابة مزرعة الدايتوم palea Nitzschia

المؤلفون: ثائر إبراهيم قاسم1
الصفحات: 168-185
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الخلاصة

The effect of essential nutrients (nitrogen, phosphorus and silicon) was tested on growth of algae (Bacillariophyceae) Nitzschia palea (Kuetz.) W. Smith under control conditions (temperature 25 ºC and light intensity 260µE/m²/sec). Modified Chu-10 was used as growth medium. The results showed that nitrate was the best for algal growth than other nitrogen sources (urea and ammonia). Four different concentrations of nitrate (15,20,30,40mg/L) as well as control medium which contain 8.7mg/L of nitrate were tested. The highest growth rate (0.55 cell/h) and the lowest doubling time (12.95 h) were recorded in 15mg/L of nitrate. The diatom was cultivated also in four different concentrations of phosphorus (5,10,20,30mg/L) as well as 1.78mg/L of phosphate in control medium. The treatment of 5mg/L phosphate showed highest growth rate (0.57 cell/h) and lowest doubling time (12.75 h). The third essential element (silicon)of the diatom was tested in five concentrations (10,15,25,35,45mg/L) within the control treatment 5.7mg/L. The best for both growth rate (0.83 cell/h) and doubling time (8.72 h) were recorded at 10mg/L. So, new medium was suggested for cultivation of this alga.


Article
HAEMATOLOGICAL STUDY OF -THALASSAEMIA AMONG IRAQI POPULATION
دراسة خصائص الدم لمرضى الثلاسيميا نوع بيتا بين المجتمع العراقي

المؤلفون: زهير محمد علي الاسدي1
الصفحات: 186-197
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الخلاصة

Blood samples of 80 clinically thalassaemic patients were collected from three thalassaemic centres in Iraq, in addition to 56 apparently healthy individuals were collected as a control group. Haematological examinations were applied for some parameters. The blood groups were determined for all samples, the relationships between blood groups and both gender and phenotypes of β-thalassaemia were studied. The results showed that there were no significant relationship at (p > 0.05) between blood groups and both parameters (gender and phenotypes) for patients in comparison with the control group. The estimations of Hb concentrations, quantitative and qualitative Hb analyses by Hb electrophoresis in order to determine the Hb types (HbF, HbA, and HbA2) and reticulocyte count, were adopted for 31 β-thalassaemic patients and 20 apparently healthy individuals. According to the haematological examinations data, the relationships of gender, blood groups, and phenotypes with Hb concentrations, Hb types, and reticulocyte count were studied.

الكلمات الدلالية

Beta-Thalassaemia --- Haemoglobinopathies --- Hb electrophoresis --- Iraq


Article
EVALUATION OF HEPATITIS B VIRUS VACCINATION AMONG CHILDREN IN BAGHDAD CITY
تقييم لقاح الكبد البائي عند الاطفال في مدينة بغداد

المؤلفون: أمنة نعمة الثويني
الصفحات: 198-209
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الخلاصة

This study attempt to determine the protective level of antibodies against hepatitis B surface antigen among vaccinated and unvaccinated children in Baghdad city, age ranging 1- 10 year. A total of 250 blood samples have been collected and tested for detection of serological markers of hepatitis B virus (HBsAg, anti-HBs and anti-HBc). They included 190 children represented the vaccinated group and 60 children represented the unvaccinated group. Prevalence of anti-HBs was higher in vaccinated children (70%) than in unvaccinated children (3.3%.) Anti-HBc was not found in vaccinated children, while 3.3% in unvaccinated children. The distribution of protective level is divided to less than 10; 10-100; and 100-more than 500 m.i.u./ml among studies groups that have anti-HBs 13(6.8%); 21(11%); and 99(52.1%) of vaccinated and (0.0%); (0.0%); and 2(3.3%) of unvaccinated group. Finally, prevalence of protective anti-HBs level according to the doses received by vaccinated group has revealed that 77.2% of those who received the three primary doses.Hepatitis, Hepatitis B virus, vaccinatio

الكلمات الدلالية

Hepatitis --- Hepatitis B virus --- vaccination


Article
MOLECULAR CHARACTERIZATION OF TWO SHIGA-LIKE TOXIN PRODUCING ESCHERICHIA COLI ISOLATED FROM MINCED MEAT IN IRAQ
التوصيف الجزيئي لعزلتين من بكتريا Escherichia coli المنتجة للذيفان المشابه للشيكا المعزولة من اللحم المفروم في العراق

المؤلفون: عبدالأمير محمد غريب
الصفحات: 210-217
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الخلاصة

Two strains of Escherichia coli O157: H7 were isolated from raw minced meat samples on sorbitol MacConkey agar supplemented with Cefixme and Tellurite (SMAC-CT) after selective pre-enrichment of the samples in enriched EHEC broth (EEB). The diagnosis was confirmed biochemically and serologically by latex agglutination. Resistance profile to antibiotics was almost similar in the two strains. Both strains were susceptible toward the probiotic Lactobacillus spp. Both contains single large plasmid but varies in their small plasmid content. The two strains contained shiga-like toxins stx1, stx2 as evidenced by amplified (224, 227 bp) DNA fragments. One strain contained eaeA gene 1087 bp amplified fragment. The results indicated that derivatives of E. coli O157:H7 containing stx may circulate in this type of local processed food. Stx genes can be targeted as molecular markers for epidemiological studies in the region.

الكلمات الدلالية

eaeA --- stx genes --- O157 --- Iraq


Article
A SPECTROSCOPIC STUDY ON EFFECTS OF BENZENE UPON HUMAN DNA IN THE PRESENCE OF
دراسة تأثيرات البنزين والاشعة فوق البنفسجية النوع باء في الحامض الديوكسي رايبوزي للانسان باعتماد المطياف الضوئي

المؤلفون: بروين هاشم سليم1
الصفحات: 218-225
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الخلاصة

The free radical generated substances like Benzene (Bz ) showed to be caused a significant (P<0.01) elevation in absorbance of human DNA mixture ( DNA , TE buffer and Bz) after previous exposure to UV-B light with different time periods in comparesom with control groups. The changes investigated with two types of UV spectrophotometer (manual and screening types) while the exposure done with manually designated UV-B light source. The changes in absorbance observed with usual UV spectrophotometer and with λ max scanners UV spectrophotometer could be attributed to formation of more doubled bonds with basic nitrogenous bases of used DNA as a sequels to the presence of free radicals which generated by Benzene and U.V.- B in aqueous solution .The elevation of absorbance reduced by cooling the mixture with 4оC after the last period of UV – B light exposure. These results reflected the dramatic changes in human DNA directly in case of presence of Benzene and UV – B light which may be explained the wide correlated diseases as well as presented a possible prophylactic method by using cooling which reduce these effects and for that may delayed the development of such diseases . Lately the research presented an easy method to investigate the possible changes in the human DNA under the circumstances of the experiment rather than these of difficult , expensive and more dangerous methods .

الكلمات الدلالية

Human DNA --- Benzene effect --- UV-B light


Article
HYDROGEN PEROXIDE EFFECTS ON IMMUNE RESPONSES (CELLULAR AND HUMERAL ) IMMUNITY OF ADULT MALE RABBITS
تأثيرات بيرو كسيد الهيدروجين على الاستجابة المناعية( الخلطية و الخلوية) في ذكور الأرانب البالغة

المؤلفون: خالصة كاظم خضير
الصفحات: 226-238
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الخلاصة

This study was designed to investigate the effects of 0.5% hydrogen peroxide H2O2 and levamizole drug on immune response of adult male rabbits. Twenty four mature male newzeland were randomly divided into two groups and were treated as follow for four weeks: Control group(C) and H2O2 treated group (GI) which were daily intubated with 0.5% H2O2 in drinking water. After cessation of H2O2 intubation (4weeks), animals of GI group where intubated (each 72 hrs) with levamizole (5mg /Kg B.W.) in drinking water for another four weeks . Blood samples were taken at zero, 4 and 8 weeks of the experiment to determine some blood and immune response parameters including: differential leukocytes count, total leukocytes count (TLC), active and T- lymphocytes, neutrophil/lymphocyte (N/L) index, platelets count , phagocytic activity and IgG concentration. The results conducted that H2O2 treated group showed significant decrease (p<0.05) in some blood and immune response parameters manifested by leucopenia, lymphocytopenia, thrombocytopenia and a significant increase (P <0.05) in N/L index with significant suppression in the percentage of active and total lymphocyte, phagocytic activity and serum IgG concentration. On the other hand, levamizole treatment leading to a significant increase (P<0.05) in the immune response parameters and a significant decrease (P0.05) in N/L index.

الكلمات الدلالية

H2O2 --- IgG --- Immune response --- ROS.


Article
FACTORS AFFECTING AERATION IN FERMENTORS WITH AIR- SUCTION TYPE IMPELLER
العوامل المؤثرة في عملية التهوية للمخمرات ذات الخلاطات الساحبة للهواء

المؤلفون: علاء كريم محمد
الصفحات: 239-249
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الخلاصة

The rate of gas induction was measured in gas-inducing type mechanically agitated contactors provided with two impellers. A reactor of 0.5 m i.d. was used with a working capacity of 60 liters of liquid. Tap water was used as the liquid phase, and air was used as the gas phase. The bioreactor mixing system consists of two equal diameter stirrers; the top impeller is shrouded-disk/curved-blade turbine with six evacuated bending blades, while the bottom impeller was disk turbine. The impeller speed was varied in the range of 50 to 800 rpm. The ratio of impeller diameter to tank diameter (D/T) and the submergence (S) of upper impeller from the top were varied. The effects of clearance of lower impeller from the tank bottom (C2) and the impeller spacing (C3, distance between the two impellers) were also varied over a wide range. Rate of gas induction (Q) was measured for all these combinations. It was found that the rate of gas induction increases with an increase in the impeller diameter and with a decrease in the impeller clearance, while it decreases as the liquid submergence increases and as the clearance from bottom increases.

الكلمات الدلالية

Fermentor --- Air-suction impeller --- Bioreactor

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