Table of content

Jornal of Biotechnology Research Center

مجلة مركز بحوث التقنيات الاحيائية

ISSN: 18151140
Publisher: Al-Nahrain University
Faculty: Presidency of the university or centers
Language: Arabic and English

This journal is Open Access

About

The Biotechnology Research Center (BRC) in a Al-Nahrain University issued in 2007 the first edition of a tightly seasonal scientific journal named as the journal Biotechnology Research Center (JBRC) which got authorization in 2005 and held an impact number ISSN: 1815-1140.The journal accepts scientific researches in Arabic and English.
JBRC’s main interest is Biotechnology researches in the Medical, Molecular, Agriculture and environmental fields which have an important impact on the public and private sectors in Iraq.
JBRC’s structure consists of editing committee (headed by the manger of the BRC and the editor in chief), consulting committee (contains a well- known iraqi scientists in biotechnology) and editing secretary.
All researches are submitted to the JBRC’s regulations which is mainly is that they must be according to the journal directions and instructions , then the researches will be evaluated by three well-known scientists in the field and after that reviewing occurred by the editing committee to ensure and verify all JBRC’s instructions and regulations are taken into consideration .
A special edition of JBCR is issued to cover all researches that presented to the BRC’s scientific conferences which subjected to all regulations and instructions of publishing in JBCR.

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Contact info

brcn2012@yahoo.comيتم الاتصال عبر البريد الالكتروني لمجلة مركز بحوث التقنيات الاحيائية


0096407707766148او للاتصال تلفونيا
بسكرتارية التحرير
م.م. سعاد محمد مجيدب
محمد منير حسين

Table of content: 2014 volume:8 issue:4

Article
Use of GADD45A and CDKN1A Gene Expression Changes as Biomarker in Assessment of DNA Damage for Ionizing Radiation Exposure
استخدام التغايرات في التعبير الجيني لـ CDKN1A و GADD45A كمؤشر بيولوجي في تقدير الضرر في جزيئه الدنا عند التعرض للأشعة المؤينة

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The present study aims to determination of GADD45 and CDKN1A expression genes as a biomarkers for ionizing radiation in white mice Mus musculus Balb/C by using the real-time quantitative PCR assay. Seventy- two white mice (36 males and 36 females) were divided into two groups; their whole body was exposed to 5 cGy and 100 cGy of X-ray radiation at a dose rate of 200 cGy/min, in addition to the control group. Total RNA was isolated using Trizol method from liver samples of mice after 6, 48 hours and 10 days of exposure to radiation as well as of the control group. Complementary DNA was used in amplification of genes used in the present study, two types of primers pairs were selected for the genes amplification Growth arrest and DNA-damage inducible A (GADD45A) and Cyclin-dependent kinase inhibitor 1A (CDKN1A), which have a relation with ionizing radiation in addition to the primers for internal control (β-actin) gene. The size amplified product were 95 bp and 162 bp nitrogen-base pair for GADD45A and CDKN1A genes, respectively. The existence of significant elevation p <0.05 in the amount of gene expression of the GADD45A gene in samples of mice liver exposed to doses 5 cGy and 100 cGy after 6 hours of exposure to radiation. It was found that this gene having up-regulation level after 6 hours in the liver of mice exposed to these doses in comparison with the control group. The presence of a significant reduction (p <0.05) in the amount of gene expression of the CDKN1A gene in samples of mice liver exposed to doses 5 cGy and 100 cGy after 6 hours of exposure to radiation and this reduction continued after 24 hours and 10 days. Moreover, it was found that this gene had a down-regulation level after 6 hours in the liver of mice exposed to these doses in comparison with the control group. The organizational level in the high dose of 100 cGy is higher than that at the low dose 5 cGy. In conclusion, the results indicated that there is a possibility of using the changes in the level of GADD45A and CDKN1A genes expression as useful biomarkers in assessment of DNA damage for low and high radiation exposure.


Article
Role of Gibberellic acid in mitigating the adverse effect of sodium chloride on some grow parameters of fenugreek plant Trigonellafoenum-graecum L. by using Hydroponic Technique
دور حامض الجبرلين في تقليل التأثير السلبي لكلوريد الصوديوم في بعض مؤشرات النمو لنبات الحلبة Trigonellafoenum- graecumL. باستعمال تقنية الزراعة المائية

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An experiment was conducted by using the nutrient solution unit in the green house of the Biology Department, College of Education Ibn Al- Haitham/ Baghdad University during the growing season of 2008-2009 by using fenugreek plant under effect of three concentrations 0,50,100 mM. Lˉ¹ of sodium chloride and four concentrations 0,25,50,100ppm of giberellic acid of studied some growth parameters of plant diameter of root, leaf chlorophyll content, number of flower and pud's,sodium and chloride concentrations in shoot The experiment was accomplished as a completely randomized design(CRD) by using three replicates including 36 plastic pots in nutrient solution unit, the results showed the increase in sodium chloride concentration from 0_100Mm.L ̄1 in nutrient solution negative effects in mentioned parameters growth above. Results also that giberellic acid showed role in decreasing the harmful effects of sodium chloride in studied parameters.


Article
Effect of Inoculation with Psudomones fluorscens bacteria and Different Levels of Phosphate rock on the Growth and Yield of the Plant Vigna radiata.L
تأثير التلقيح ببكتريا Psudomones fluorscens ومستويات مختلفة من صخر الفوسفات في نمو وحاصل نبات الماشVigna radiata. L

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The pots experiment has been conducted to investigate the effect of inoculation of Psudomones fluorscens bacteria with two levels of Phosphate rock (60 and 120 Kg. ha-1) on growth and yield of livestock plant Vigna radiate. L. Two strains of Psudomones fluorscens that dissolves phosphor have been isolated from the rhizosphere area of okra and corn crops which are symbolized as P1 and P2. xpectivly results revealed surpass bacteria strains over the example strain in most characteristics and P1 has surpassed P2 in the studied chriteria as the rate of nodes (4.67 node plant-1) and the dry weight of the nodes has come to 12.63 mg plant-1. The strain P1 has surpassed in the number of pods and the number of seeds in a pod and the rates of (11.33 node plant-1) and (6.78 seed pod-1) have been recorded. The results have showed the surpassing of the level (120 Kg ha-1) over the level( 60 Kg ha-1) in most studied chriteria as the dry weight of the greenery total recorded by the level (120 Kg ha-1) has been (3.56 gr plant-1) and the rate of the absorbed Nitrogen amounts to 7.41 mg gr.-1 while the rate of the absorbed phosphor is( 1.01 mg gr.-1). The of pods that the level (120 Kg ha-1) has recorded is( 11.0 pod plant-1) . The interaction treatment between the bacteria Psudomones fluorscens and phosphate rock has surpassed in all studied characteristics. The interaction treatment between the strain P1 and the level (120 Kg. ha-1) has recorded the highest rates and in most characteristics as it has recorded the highest rates in the number of root nodes and its dry weight which amount to (10 node plant-1) and( 26.89 mg plant-1). It has also recorded the highest rates in the number of pods and the plant yield of seeds which amount to(13.0 pod plant-1) and (2.1 gr plant-1) respectively.


Article
Study of Protective effect of Glutathione Peroxidase (GSH-Px), Vitamin E and Selenium on Iraqi children with Leukemia
دراسة التأثير الوقائي للكلوتاثايون بيروكسيديز GSH-Px وفيتامين E والسلينيوم Se على مرضى سرطان الدم في أطفال العراق

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The present study have been investigated the levels of antioxidant substances which including the enzyme glutathione peroxidase GSH-Px, vitamin E (α- tocopherol), and selenium, in order to estimate their roles of preventing the body cells against oxidation processes by free radicals, hydrogen peroxides, hydrofattyperoxides, reactive oxygen species, which attack the cell membrane causing damage of these cells resulting in mutation of DNA and accidence of various cancer diseases such as leukemia. By studying 30 female and 30 male, 2-14 years old, they were divided into three groups, each group formed 20 patients. These groups included two kinds of leukemia are acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The concentration level of glutathione peroxidase GSH-Px, and concentration level of α- tocopheroland selenium were significant P< 0.05 decrease in all groups of patients male and female in both kinds of leukemia's when compared to control group.


Article
Micro propagation of four Potato Cultivars Solanum tuberosum L. in vitro
الإكثار الخضري الدقيق لاربعة اصناف من البطاطاSolanumtuberosumL. خارج الجسم الحي

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Vegetative buds excised from tubers for four varieties of potatoes namely: Arnova, Brovento, Rivira and Bureen (after breaking the dormancy phase) which sterilized with 2% Sodium hypochlorite for 10 minutes and cultured in the MS medium supplemented with 0.5 mg/l of IAA or IBA with different concentration of BA, as well as control treatment in the initiation stage, while different concentrations of BA (0.0, 0.5, 1.0, 1.5, 2 mg/l) with 0.5 IAA mg/l in multiplication stage. All cultures incubated at a temperature of 2 ± 25º C and an illumination of 1000 lux for 16 hours/ day. Data of mean number of branches shoot per plant and their length and leaves for two stages were investigated. The results showed that BA + IAA at 0.5 mg/L for each one, gave the highest initiated percentage, shoot per plant ,length, reached 75%, 1.5 shoot / bud, 2.21cm and 3.88, leaf/ bud, respectively. Results of multiplication indicated that there was a significant interaction effect of both cultivars and BA concentration on all characterizes, Based on this interaction, Arnova cultivars at 1.5 mg/L BA showed the highest mean of shoot per plant (4.0) While Rivera cultivars at 0.5 mg/L BA gave highest mean of length, leaves per plant reached 6.45cm,7.75 leaf/plant respectively.


Article
HLA Polymorphism in a Sample of Iraqi Acute Lymphoid Leukemia Patients
تعدد الأشكال الوراثي لمستضدات خلايا الدم البيض البشرية في عينة عراقية من مرضى ابيضاض الدم اللمفي الحاد

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The present study aimed to investigate the association between HLA class I (A, B and Cw) and class-II (DR and DQ) antigens and acute lymphoid leukemia (ALL) in Iraqi Arab patients (45 subjects). Based on their clinical subtypes the patients were divided into two subtypes, which were L1 (17 patients)and L2 (28patients). The patients were admitted to Baghdad Teaching Hospital and National Centre of Haematological Diseases at Al-Mustansiryiah University for diagnosis and treatment during the period May 2004 - October 2005. Control sample (blood donors) of 180 subjects, matched with patients for ethnic background (Arab Muslims) was also included. The total ALL patients showed significant increased frequencies of A2 and B12, and a similar observation was made in L1 and L2 subtypes in addition to CW3 The co-occurrence of two antigens belongs to two HLA loci in the same individual was also inspected 14.8,48.1,12.9,9.3, and then their percentage frequencies were determined. Such determination revealed that there were four allelic combinations, which showed a significant increased frequency in the patients. They were A1-DR7, A2-B12, A9-B12, and CW7-DQ1. The RR values of such deviations were 7.65, 26.93, 13.26, and 18.27, respectively of sample Iraqi patients.


Article
Reusing of Treated Wastewater by Membrane Bioreactorin Irrigation
إعادة استخدام مياه الصرف الصحي المعالجة بمفاعل الأغشية الحيوية في الري

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Membrane Bio Reactor(MBR) methods is considered as one of the most advanced used technology in waste water treatment. The aim of this technology is to obtain high quality treated waste water. This study conducted to investigate the ability of using of sewage water from zaafrania city for irrigation and their effect on corn growth and some of soil chemical properties which include electrical conductivity and soil pH in extract soil paste and the micro nutrient content in soil and plant which include Fe, Mn, Zn, Cu, Cd, Pb. Three levels of wastewater were used 0, 50, 100% in two stages, the three levels of wastewater ( without soil fertilization ) were used in the first stage, where 21.8 Kg P/D +80 N Kg/D was added to the soil as fertilizer in the control 0% treatment and 10.9Kg P/D +40 Kg N/D were added to 50 and 100% levels in the second stage. Corn seeds were planted in 10kg plastic pots in Completely Randomized Block Design in three replicates. The results refer to a high significant increase in plant height, fresh and dry weight for all treatments in comparison with control treatment. The low add level of wastewater 50%+ half of fertilizer recommendation gives a significant increase of plant height and fresh and dray weight. The results showed a high increased of electrical conductivity for 50, 100% wastewater added levels for both stages compared with control treatment. The high add level 100% gives high significant increase of electrical conductivity compared with the low level of the wastewater. Whereas the values of soil PH were close to the neutral for all treatment. The result showed a significant increase in micro nutrients content (which include Fe, Mn, Zn, Cu, Cd, Pb) in soil and plant for all treatment compared with control treatment, this increased was continues with the increase of additional level of wastewater. However all the micro nutrient was within the allowed natural limits and not reached the toxic limits in soil and plant.


Article
Study the Antimicrobial effect of Kombucha tea on bacteria isolated from Diabetic foot ulcer
دراسة التأثير المضاد للمكروبات لشاي الكمبوشا على عزلات جرثومية من قرح القدم السكري

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The study was conducted from January to March 2012. In this study colorimetric VITEK-2 Compact system used for its accuracy and rapidity to identify isolates and to detect several antimicrobial resistances.The study also investigate the antibacterial effect of Kombucha tea on isolated bacteria from diabetic foot ulcer. The bacteria isolated were eight gram negative bacteria, namely, Acinetobacter baumannii 3 (2%), Enterobacter cloacae 5 (4%), Escherichia coli 13 (10%), Klebsiella pneumoniae 7 (6%), Citrobacter spp. 4 (3%), Proteus mirabilis 3 (2%), Proteus vulgaris 3 (2%) and Pseudomonas aeruginosa 44 (35%). Four gram positive bacterium, Enterococcus faecalis 6 (5%), Staphylococcus aureus 17 (13%), Staphylococcus epidermidis 13 (10%) and Streptococcus spp. 9 (8%). The antimicrobial activities of antibiotics showed that, all isolates are sensitive to Ciprofloxacin, Levofloxacin and Ofloxacin. The resistance to other types of antimicrobial differ with different isolate. The effect of Kombucha tea on all isolates wasclear at 7days of incubation; the diameter of inhibition was 6mm for Acinetobacter baumannii, Proteus vulgaris and Enterococcus faecalis. 7mm for Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Citrobacter spp., Staphylococcus epidermidis and Streptococcus spp. 8mm for Proteus mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus. The maximum activity of fermented tea was recorded at 14days incubation of Kombucha organism against all isolates, the diameter of inhibition was 21mm for Acinetobacter baumannii, 24mm for Enterobacter cloacae, 23mm for Escherichia coli, Staphylococcus epidermidis and Streptococcus spp., 16mm for Klebsiella pneumoniae, 22mm for Citrobacter spp. and Enterococcus faecalis, 25mm for Proteus mirabilis and Staphylococcus aureus, 20mm for Proteus vulgaris, 26mm for Pseudomonas aeruginosa. The antibacterial activity of Kombucha tea decrease with increase incubation periods28 days.


Article
Effect of soaking and germination on approximate composition and certain characterize of isolate protein (solubility and degree of hydrolysis) Of mung bean Vigna radiate
تاثير النقع و الانبات في التركيب التقريبي وخاصيتي الذوبانية ودرجة التحلل للبروتينات المعزولة في بذور الماش

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Four groups of local mung bean Vigna radiate were soaked for 24h. Three of these were germinated for 1, 2 and 3 days at room temperature 23˚Cusing wet cloths between technique while the fourth was depending as a soaking sample in addition to control treatment without soaking. Approximate chemical composition (crud protein, oil, ash, moisture, fibers and carbohydrates) and mung protein isolates (MPI) with its solubility SP and degree of hydrolysis DH, were determined for four dehulling groups besides control sample. Dehulling mung bean DMB flour contained 23.53, 1.83, 3.11, 7.5, 17.14 and 46.89% crud protein, oil, ash, moisture, fibers and carbohydrates, respectively. While the values of MPI, SP, and DH were 12.85, 1.5 and 1.4 % respectively .During germination significantly increasing in (CP) (MPI) and (DH) and the maximum values were 25.29, 15.34 and 2.11%respectively after two days, while maximum value was 9.53% for protein solubility after 3 days of germination. While oil content diminished a little and slight inconstant changes were shown in fibers and rest carbohydrates.


Article
Effect of Aspirin on Sperm Specification and Some Hematological Parameters in Male Albino White Rat
تــأثير الأسبرين على مواصفات النطف وبعض قياسات الدم في ذكور الجرذان البيضـاء

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Thirty male albino rats were divided into three groups, the first group was given aspirin at a dose of 25 mg / kg orally and twice a day, the second group dosage was 125 mg / kg at the same way, the third set group which considered as control was treated with physiological saline, each group took 0.5 ml amount. The period of experience took four weeks at the end of the experience, in the day 29the animals were anesthetized and eradicated the epididym is and calculated the total number of sperm and percentages of viable and dead sperm as well as the percentages of motile and non-motile sperm, the blood was taken for hematological tests, results confirmed the existence of a decreased significant value (P<0.01) in the total number of sperm of the 2nd group compared with control group and the decline value was less significant (p<0.05) in the first group compared with control group. In addition a significant value (P<0.01) increased in the percentage of dead sperm and non-motile sperm, also a significant value (p <0.01) decreased in the percentage of viable sperm and motile sperm, while the results proved the existence of a significant value (p<0.05) reductions in PCV, Hb, TWBC and monocyte values compared with control group. effect of aspirin on the male reproductive system may be due to being a Prostaglandins inhibitor, and These findings probably indicate that aspirin have deleterious effect on the blood chemistry of male albino rats.


Article
Detection of biological active substances and lipase inhibitors from aqueous extracts of some legumes in comparison with Orlistat drug
التحري عن المواد الفعالة بايولوجيا ومثبطات انزيم اللايبيز البقري في المستخلصات المائية لبعض البقوليات ومقارنتها مع فاعلية عقار الاورلستات التثبيطية

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This research was aimed to determine biological active substances qualitatively in vicia faba, lentil, green peas, soybean, beans, redbean. Lipase inhibitors were screened and compared with orlistat drug. All cold and hot extracts contained both saponin and tannin while polyphenol did not exist in vicia faba and soybean; rating found in all extracts except greenbeas. Tween 80 was used in lipase and lipase inhibitors screening; lipases activity were detected at cold aqoueus extracts of vicia faba, lentil, redbean and beans while heat stable lipase detected at vicia faba hot extract only. Extracts of Green peas, soy beans and chick pea lacking lipase activity. Qualitative and quantitative determination of lipase inhibitors activity showed that soybean and chick pea gave the highest lipase inhibition. Each extract caused 100% inhibition with 472.5 unit/ml compared with 85% inhibition for 12 mg/ ml of orlistat.


Article
Study antioxidant activity in Thalassemia Iraqi patients
دراسة الفعالية التأكسدية عند مرضى الثلاسيميا العراقيين

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The aims of this study was to estimate the total antioxidant activity level in serum among the Thalassemia Iraq patients. We estimated total free radical scavenging activity in the120 serum of Thalassemic patients their age range from 2-27years. The DPPH activity of serum ranged from 0.1-3.11µmol/ml, the mean serum level of total antioxidants in Thalassemia patients was significantly lower than the control group, and no relation to age or sex. We concluded that DPPH is useful for evaluating the total antioxidant capacity.


Article

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The effects of aqueous extracts of broccoli plant against liver enzymes (ALP, ALT,AST,GST) in the laboratory animals treated with carbon tetrachloride were studied. Conducted tests on the overlap between the three concentration 100, 200,300 mg / kgm of aqueous extract of broccoli and 3.2 mg / kgm of carbon tetrachloride with interaction included two types of treatment (pre- ccl4 and post-ccl4) through oral dosage and for a period of 7 days. The study shows that the concentration 300mg / kgm is the best concentration of aqueous extract there was used and study suggests that use the concentrations of this focus and fact that the plant is used for human consumption broadly.


Article
Effect of Magnetotron on the Rates of Growth and Fecundity in Ostracoda Cyprislaevis O.F Müller,1776 under the Laboratory Conditions
تأثير المغنايتوترون في معدلات النمو والخصوبة في الدرعي Cyprislaevis O.F.Müller,1776 تحت الظروف المختبرية

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The Magneototron technique considers as recent and advanced techniques in magnetic water treatment, which application in different; ecological, agricultures, industrial, medical and scientific fields. Therefore this study making to determine the effects of Magnetotron system with different intensities 0.05,0.10 and 0.15 Tesla on some biological aspects for one species of freshwater Ostracoda cyprislaevis O.F. Müller, 1776 for economical important, because both juveniles and adults of fishes feeding on it and as a tool for water cleaning, which consideration feeding on dead and decay animals and vegetables The samples of Ostracoda were collected from Al-Jaesh canal- Baghdad province for period from 1/9/2012 to 1/11/2012.The obtained results compared with this species of Ostracoda which lived in raw water (unexposed to magnetotron system).The present study demonstrated increased significance the rates of growth by increased the bivalve carapace dimensions (length and width) for both ostracodean males and females with increased intensity of magnetotron system comparative with untreated water. Although increase significant observed in fecundity in ostracodean females by increase the number and diameter of eggs with increased intensity comported with untreated females. Also this study detected to predominant females upon males in both treated and untreated water with magnetotron system. Then the magnetotron system does not effect on heterogonous in sexual formation for this species in same ecosystem.


Article
Production of inulinase from Rhizopusoryzae by solid state fermentation
انتاج انزيم الانيولينيز من الفطر Rhizopusoryzae بواسطة تخمرات الحالة الصلبة

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Fifty Rhizopus spp. isolates were obtained from local natural habitat. The ability of inulinase production by these isolates was screened. The isolate RC-2 which isolated from composite plants was the highest inulinase producer on modified Czapek-Dox agar in primary screening. Secondary screening revealed that the same isolate was the highest production on Nakamura broth medium. Isolate was identified as Rhizopusoryzae. Measurements of reducing sugars in crude filtrate which represent the products of enzyme revealed that Rhizopusoryzae RC-2 produced inulinaseextracellular. The optimum culture conditions for inulinase production by solid state fermentation included a mixture of Jerusalem artichoke Helianthus tuberosus tuber with sugarcane Saccharum sp. bagasse with a ratio (1:1) as carbon source, 2.5% of corn steep liquor as nitrogen source, moisturizing ratio (3:1) (v:w) with tap water, the best inoculums rate was 2.5×105 spore/ml and incubation at (30)ºC for 4 days.


Article
تقييم بعض التأثيرات الوراثية- الخلوية لمستخلص الهكسان لنبات القطب Tribulus terrestris داخل وخارج الجسم الحي

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The present study was carried out to evaluate the cytogenetic effects of the hexane extract of the plant Tribulus terrestris fruits. The cytogenetic evaluations involved mitotic index of bone marrow and spleen cells in albino male mice, micronucleus formation and sperm-head abnormalities. The micronucleus evaluations were further explored in blood lymphocyte cultures of healthy donors through an in vitro study. The search was carried out through three stages. In the first, the cytogenetic effects of three doses 5,10 and 20 mg/kg) of the plant extract were evaluated, while in the second stage, interactions Pre- and Post-treatments between the ideal dose 5 mg/kg of the plant extract and the drug Mitomycin-C (MMC). In stage three, the micronucleus formation was evaluated in the lymphocyte cultures of healthy individuals after treatment with three concentrations (5, 10 and 20 µg/ml) of the plant extract, in addition to interactions with the drug MMC which revealed significant mutagenic actions as judged by the investigated parameters. Reduced mitotic index, and increased frequencies of micronucleus formation (in vivo and in vitro) and sperm-head abnormalities were observed. The first stage revealed that the plant extract reduced the spontaneous formation of micronuclei and sperm-head abnormalities. The ideal dose 5 mg/kg of the plant extract was effective in modulating the mutagenic effects of the drug MMC. In this regard, the pre-treatment was more effective than post-treatment. The stage three showed that the plant extract was effective in reducing the spontaneous, as well as, MMC induced formation of micronuclei in lymphocyte cultures.


Article
The inhibition of genotoxicity effect of Methotrexate(MTX) in human lymphocyte in vitro using aqueous extract of Rosmari nusofficinalis
تثبيط الاثر السمي الوراثي للميثوتركسيت في الخلايا اللمفاوية (خارج الجسم الحي) باستخدام المستخلص المائي لإكليل الجبل (الروزماري) Rosmari nusofficinalis

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In vitro experiments were designed to investigate the role of aqueous extract of Rosmari nusofficinalisin the inhibition of genotoxic effect of methotrexate (MTX) in human lymphocyte. Mitotic index MI, chromosomal Aberation CA used us cytogenetic tests to detect the inhibition of genotoxic effect of Methotrexate using two concentration. The results showed that the aqueous extract of Rosmari nusofficinalis inhibited the genotoxic effect of Methotrexate in human lymphocyte. As well as the exposure of cells to aqueous extract before expose to Methotrexate T1 had a high inhibitory efficiency comparing with the combined exposureto the extract & Methotrexate together T2 and the exposure to the Methotrexate before extract. According to our obtained results it could be the aqueous extract of Rosmari nusofficinalis considereds as a antimutagene agent (Desmutagen) in first place and as a antimutagene agent (Bioantimutagene) in second place.


Article
The toxic effect of the malathion pesticide on laboratory animals
التأثير السمي لمبيد الملاثيون على الحيوانات المختبرية

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The research aimed to illustrate enzyme level such as liver and immune enzymes to detect toxic effect of chemical agriculture pesticides as biological indictors to chemical pesticides contamination. 102 laboratory mice were used as a model 20-25gm weight. 30 mice were used to determine LD50 which was 30 mg/mouse. Mice were divided in to three groups according to LD50. After exposed to three different concentration by three ways (Interaperitoneal,Oraly,and Spray). Results showed significant increased in enzymes levels of (ALP), (GOT), (GPT), and (ADA) after 7 days of the three different exposure, while after 14 days there was decreased in enzymes levels of (ADA), (GOT), and(ALP) with increases in (GPT) enzyme: according to use "interaperitoneal injection and spray method'', that companied by the appearance of gross pathological lesions in mice organs represented by hypertrophy of liver and there is many cysts filled with pus which related with increased in pesticide concentration and duration of exposure in addition to bile duct obstruction to some exposed group. The results show the possibility of the adoption of variation in the level of enzymes to give an idea of the type and the impact of the pesticide, which was subjected to the animals.


Article
Using Serial Fungal Bioreactor for Reducing the Colored Dyes Wastewater
استخدام الكتلة الحيوية لنوعين من الفطريات بطريقة المفاعل الحيوي المتسلسل في خفض الملوثات الصبغية الملونة

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Serial fungal bioreactor which contained two species of fungi, Trichoderma harzianum and Penicillium spp. were used to reduce the colored dyes wastewater in the textile factories in Al-Kadhmia city/ Baghdad. Different biomass of two fungi species which cultured in the laboratory ranged five and ten grams for each treatment the colored dyes wastewater for both blue and red dyes that used in cotton textile factories. The activity of bio-reduction was increasing during multi-stages of fungal bioreactor due to more decreasing of colored wastewater of textile dyes specially when happening some specific changes in its processes. Another environmental factors were measured such as absorbance, transmission and turbidity before and after treatment. Positively relationship of fungal biomass quantities were recorded extrusive relationship in bio-reaction processes forreducingthe colored textile wastewater treatment by serial fungal bioreactor.


Article
Effect of growth regulator on in vitro potato growth var. Arnova and Borin
تاثير منظمات النمو على نمو صنفي البطاطا ارنوفه وبورين خارج الجسم الحي

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This study was focused to test the effect of BA concentrations 0, 0.5, 1, and 2 mg/l on multiplication of two potato varieties, namely Arnova and Borin. In vitro microtubers production was tested by adding 4 mg/l of BA or kinetin with 80 gm sucrose/l. Results of multiplication indicated that there were significant differences between genotypes in leaves number. Studied of BA concentration and interaction between cultivars and BA revealed that there were significant effect in plant height, branch length/plant, leaves number/plant and cut number/plant. In case of microtubers induction, the result showed that there were no significant effect of varieties and plant growth on number and diameter of microtubers.


Article
Histopathological changes resulted exposure the common carp fish Cyprinus carpioL. to diazinon pesticide
التغيرات المرضية النسجية الناتجة من تعرض اسماك الكارب الاعتياديCyprinus carpioL. لمبيد الديازينون

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Abstract

In the present study, the effects of diazinon on Cyprinus carpio L. were investigated according to the following parameters: determination of median lethal concentration (LC50), gross pathological lesions and histopathological changes. To simulate the situation in the nature regarding the possible mode of exposure of fishes to pesticide, experiments of acute and accumulate subacute exposures were designed. For acute exposure, groups of fish were exposed to 0.025, 0.050, 0.100, 0.150, 0.200, 0.250 and 0.300)ppm, respectively for 72 hrs. at 15 C° and pH7.5 to simulate the subacute exposure, a group of fishes were exposed to 0.006 ppm as an accumulated concentration during 15 days. The most prominent results was as following the LC50 was found to be 0.150 ppm. Gross lesions and microscopically examination of tissues of acutely or chronically exposed fish after 3, 15, 30 and 45day from the end exposure was conducted the examination revealed histopathological changes in (gills, livers and kidneys) damage of all the tissues were observed as inflammatory response of fish to get rid of the toxic material also proliferation of bile duct in liver was observed.


Article
The genetic effect of N-methyl-N-nitro-N-nitrosoguanidine on callus induced from mature embryos of beans Phaseolus vulgaris
الكشف عن التأثير الوراثي للمطفر الكيمائي نايتروزوجواندين في الكالس الناشئ من ألأجنة الناضجة لنبات الفاصوليا Phaseolus vulgaris

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Abstract

This research was conducted to study the effect of the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine (NTG)on the chromosomes of callus induced from mature bean embryos, Harvester cultivar. Seeds were treated with 0.2 or o.4 mM of the mutagen that mixed with different percentages of ethanol for 24 hrs. Calli were induced on MS medium in the presence of 0.5 mg/L of Benzyl adenine (BA), 1 mg/L Indole acetic acid (IAA) and 100 mg/L from each of Casein hydrolysate, Glycine, Asparagine, Tyrosine, and Myo-Inositol. Samples were pretreated with 1, 2- benzene dichloride, Para –dichlorobenzene, or Colchicine. Two different staining methods were used to stain the chromosomes from root tips and calli.The results showed that Para –dichlorobenzene is the best pretreatment for both root tips and callicells. However, the stain acetoorcein was the best for the root tips while Feulgen stain was the best for calli cells. Chromosome count showed that there were 22 chromosomes in all the cells of bean root tips (control). While a wide range of chromosome numbers were obtained from calli cells with or without mutagen treatment. Ninety six percent of the non treatedcalli gave the normal number of chromosomes while only 60% of calli treated with (0.4 mM+4% ethanol) gave the normal number of chromosomes. Calli cells from all the treatments showed chromosome multiplication except in the presence of ethanol.

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