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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2015 المجلد: 14 العدد: 1

Article
Epigenetics, Biotechnology, and Other Scientific Advances: The Role of Iraqi Researchers

المؤلفون: May R. Talha Arnaot
الصفحات: 1-8
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الخلاصة

Abstract: The past two decades has witnessed an amazing outburst of scientific developments and discoveries accompanying the so-called Genetic Revolution. The advances that followed the deciphering of the Human Genome in 2003 particularly in the molecular biology and genetics were not possible without parallel developments in the biotechnologies and the bio-informatics. The new science of Epigenetics was developed in the aftermath with surprising revelations. In this article we will be examining the relevance of these developments to the Iraqi research and the problems of environmental pollution 1991-2004 with the consequent rises in cancer and birth defects. We submit a new theory that birth defects in Iraq are more likely to be caused by Epigenetic factors than carcinogenetic factor and we call for a well oriented and problem targeted planning in scientific research in Iraq. The successful themes in the scientific research of the 21st century are the culture of team work and co-operation and the integration of expertise among specialists. The American example of a highly successful, well planned and problem oriented team research work that lead to deciphering the human genome and the development of the science of epigenetics is discussed. The role of governmental support is illustrated. Recommendations and suggestions are made in the coarse of developing a problem oriented scientific research in Iraq such as to allow the Iraqi scientists to be ahead of other scientists in solving the Iraqi problems.


Article
Improving of Antibacterial Activity for Antibiotics by Purified and Characterized Lectin from Acinetobacter baumannii

المؤلفون: Sahira Nsayef Muslim
الصفحات: 9-22
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الخلاصة

Abstract: Lectin has a wide range of applications in many fields as anti-tumor, anti-insect , antiviral and antifungal drug. Here we reported lectin production from Acinetobacter as there is no report on lectin production from Acinetobacter. In the present study a novel strain, Acinetobacter baumannii S3 isolated from sputum samples gave the highest production level of lectin by microscopic glass slide and microtiter plate methods and the hemagglutination activity was not blood-type specific. Lectin was purified to homogeneity with a recovery yield of 72.72% and 40.76 fold of purification by using ammonium sulfate at 35% saturation followed by QAE-Sephadex ion exchange chromatography and sephadex G-200 gel filtration chromatography. The optimum activity of purified lectin was found at 30ºC and pH 7. The best cofactors for lectin activity were Mn++ and Mg++ with relative activities of 132 and 122%, respectively and severely inhibited in presence of Fe3+, Cu2+and detergents. Lectin led to increase antimicrobial activity of antibiotics and had synergism effect, thus lectin may be useful adjuvant agent for the treatment of many bacterial infections in combination with antibiotics.

الكلمات الدلالية

lectin --- Acinetobacter baumannii.


Article
Molecular Typing of Staphylococcus aureus by DNA Restriction Fragment Length Polymorphism of coa Gene

المؤلفون: Kawthar R. Lateef1 , Ismail H. Aziz2, Ashna J. Faik1
الصفحات: 23-28
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الخلاصة

Abstract: The aim of this study was to determine the genotypic characteristics of S. aureus isolates by using coagulase gene typing and polymerase chian reaction-restriction fragment length polymorphism (PCR-RFLP) of coagulase gene. One hundred and seventy two wound swab specimens were collected from patients in AL-Kindy Teaching Hospital, Al-Wasity Hospital, Baghdad Al-Taalemey Hospital and Al Shaheed Gazi Hospital in Baghdad during the period from September 2013 to January 2014. All isolates were diagnosed depending on microscopical and biochemical tests. It was found that 47(27.33%) isolates were identified as S.aureus. The amplification of the coa gene of S.aureus strains generated 5 different genotypes: I, II, III, IV, V based on different size of polymorphism raning from 500-900bp. The majority (44.7%) of the strains were of coa gene type II. PCR-RFLP of coa gene exhibited 11patterns which were obtained with AluI digests of PCR products. The number of fragments varied from one to three with sizes of the fragments varied from 80 to 700 bp. Type IX was the most common and accounted for 27.7% of the isolates. PCR-RFLP analysis, showed discriminatory power, reproducibility, easy interpretation and can be considered as a promising alternative for the epidemiological typing of S.aureus isolates.

الكلمات الدلالية

Staphylococcus aureus --- molecular typing --- Coa gene --- PCR- RFLP.


Article
Estimation of Genetic Diversity and Proline Content in Some Iraqi Bread Wheat Cultivars

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الخلاصة

Abstract: The genetic diversity of six Iraqi bread wheat cultivars: (Dijlah, Furat, Ibaa-99, Inia-66, Uruq and Tamooz 2) were studied with three treatments of salt stress (0,5 and 10ds/m). The RAPD technique was used to estimate the genetic diversity. Salt stress pattern was used. RAPD data by using cluster analysis revealed that Dijlah and Furat cultivars are closed to each other and behave as one group, and Inia-66 and Uruq cultivars are closer to each other because they are sharing the same origin. Ibaa-99 cultivar was nearer to the second group (Inia-66 and Uruq). Under salt stress condition, differential response in term of leaves proline content was observed, and it is ranged between (15.16 to 6.55 M mole/g). The results showed that there is a genetic diversity among mentioned cultivars with relation to the proline accumulation by salt stress condition.

الكلمات الدلالية

Wheat --- Proline --- Diversity --- Stress.


Article
Desulfurization of Dibenzothiophene by Pseudomonas aeruginosa Isolated from Iraqi Soils

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الخلاصة

Abstract: A dibenzothiophene (DBT) utilizing bacteria, as the sole source of sulfur, were isolated from soil samples contaminated with hydrocarbons. In this study, an effective enrichment technique was applied to isolate bacterial isolates with capabilities to utilize dibenzothiophene (DBT) as a model compound of polyaromatic sulfur heterocyclic compounds (PASHs). From forty samples, sixty three bacterial isolates were obtained and three of them were characterized as biodesulfurising Pseudomonas aeruginosa. A convenient spectrophotometric assay (Gibbs’ assay) was used to determine the quantity of desulfurized product (Hydroxybiphenyl HBP) by these isolates, also these isolates were capable to utilize ethanol as C- source.

الكلمات الدلالية

Biodesulfurization --- dibenzothiophene --- Pseudomonas aeruginosa --- Gibbs’ assay


Article
Detection of Codon 12/13 g.6262G>A Mutation of H-ras Gene in Iraqi Bladder Carcinoma Patients

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الخلاصة

Abstract: DNA was extracted from blood and urine samples from 45 patients with bladder carcinoma (age 20-87 years) in addition to samples from 25 apparently healthy persons as controls. Restriction fragment length polymorphism (RFLP) analysis was performed to determine genotypes of the H-ras codons12,13 using Msp1 enzyme. The healthy results showed that two fragments (165 bp and 55 bp) were produce from the digestion with the enzyme for H-ras codon 12/13. These results indicated that the PCR amplified region of the codon 12/13 has one restriction site for the enzyme Msp1. The molecular analysis of the patient samples revealed that among 45 patients included in this study, 28 patients (62.2%) were with normal pattern (165 bp and 55 bp) and 17 patients (37.8%) were homozygous mutants (g.6262G>A). The frequency of g.6262 C>G mutation in patients was significantly higher than in apparently healthy subjects (37.3% versus 0%, OR= 0.033; X2=0.966*, P<0.01).

الكلمات الدلالية

Bladder carcinoma --- H-ras --- MSP1 --- RFLP --- g.6262G>A


Article
Detection of 16S Ribosomal RNA Methylation in Extended-Spectrum β-lactamase-Producing Klebsiella Pneumoniae Clinical Isolates from Baghdad Hospitals

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الخلاصة

Abstract: Fifty-eight Clinical isolates of Klebsiella pneumoniae were collected from patients at Al-Kindey teaching hospital, Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad from Urinary tract infection (UTI) patient. These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E, isolates all screened for antibiotic resistance by rapid detection of β-lactamases production method and their minimum inhibitory concentrations(MICs) were conducted by polymerase chain reaction assay. Detection of blaCTX –M gene was performed and highly resistant isolates (n=17) were selected to determine aminoglycoside resistance mediated by methylation 16S rRNA that combined with the detection of blaCTX –M gene responsible for Extended Spectrum β-lactamase (ESβLs) production. Seven 16S rRNA methylase genes were amplified, the ArmA, RmtA, RmtB, RmtC, RmtD, RmtF and npmA beside amplifying blaCTX –M gene. This study explained the physically link between blaCTX-M gene (ESβLs) production among Klebsiella pneumoniae in correlation with 16S rRNA methylation.

الكلمات الدلالية

Klebsiella pneumoniae --- ESβLs --- blaCTX-M gene --- 16 rRNA methylation genes.


Article
Circulating Human IL-10 - Secreting Regulatory B Cells in Acute and Chronic Ischemic Heart Disease

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الخلاصة

Abstract: Ischemic heart disease (IHD) is a leading cause of death worldwide. Suppression of immune system after the resolution of infection or inflammation is an important process that limits immune-mediated pathogenesis, therefore, in this study for the first time in Iraq we highlights the importance of IL-10 secreting regulatory B cells (B10/Br1/BREG) in ischemic heart disease immunoregulation. Peripheral blood lymphocytes were isolated from 83 patients with ischemic heart disease, then IL-10 secreting regulatory B cells were detected by using double staining immunocytochemistry (DS-ICC) with both CD19 and IL-10 monoclonal antibodies. Results showed the decreased number and mean percentage of BREG in the peripheral blood of both acute and chronic ischemic heart disease in general when compared with controls, but these cells recorded higher number and mean percentage in acute than chronic ischemic heart disease, and there was a significant difference in the mean percentage of BREG cells among the patient's groups. In conclusion, IL-10 secreting regulatory B cells play an important but limited role in ischemic heart disease immunoregulation due to their lower numbers, and further studies must be done for future directions correlated with how to manipulate these cells in immunoregulation with more advanced pathways.

الكلمات الدلالية

IL-10 --- B cell --- CIH --- DS-ICC --- IHD.


Article
Association of Glutathione S-Transferase (GSTM1, T1) Gene Polymorphisms with Type 2 Diabetes Mellitus (T2DM) in the Iraqi Patients

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الخلاصة

Abstract: Diabetes mellitus is associated with an increased production of reactive oxygen species (ROS) and a reduction in antioxidant defense. The oxidative stress becomes evident as a result of accumulation of ROS in conditions of inflammation and Type 2 diabetes mellitus (T2DM). The genes involved in redox balance, which determines the susceptibility to T2DM remain unclear. In humans, the glutathione S-transferase (GST) family comprises several classes of GST isozymes, the polymorphic variants of GSTM1, T1 genes result in decreased or loss of enzyme activity. Aims: The present study evaluated the effect of genetic polymorphisms of the GST gene family on the risk of developing T2DM in the Iraqi patients. GSTM1, T1 polymorphisms were genotyped in 25 T2DM patients and 25 healthy controls from Iraq to analyze their association with T2DM susceptibility. Materials and Methods: Analysis of GSTM1 and GSTT1 gene polymorphisms was performed by multiplex polymerase chain reaction (PCR) .There was significant association in the GSTM1 gene polymorphism and Type-2 Diabetes Mellitus (P<0.05). Moreover, significant relationship was found between the polymorphism of GSTT1 genes and higher risk of Type-2 Diabetes Mellitus among Iraqi subjects (P<0.05).


Article
Isolation of Uropathogens from Pediatric Associated UTI, with Special Focus on the Detection of Proteus Vulgaris

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الخلاصة

Abstract: The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014. Mid –stream urine of 75 patients were cultured. Out of these 67(89.33%) patients` urine were positive for bacteriological culture. The distribution of infection was 50.7% infants aged 2-9 months and 46.3% children aged 1-4 years. Total of six bacterial species isolated were E.coli 35(52.2%), Enterobacter spp. 16(23.9%), Proteus spp.11 (14.9%), Klebsialle spp. 3(4.7%), Pseudomonas spp. 1 (1.5 %) and Staphylococcus spp. 1(1.5 %). The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized. PCR technique was used to detect Proteus vulgaris by using species – specific primers Urease C, the results of primer urease C observe 3 isolate were positive. Rapid diagnosis of the pathogen in a clinical sample is always very important.

الكلمات الدلالية

uropathogens --- pediatric --- UTI --- proteus vulgaris --- urease C


Article
Detection of E. coli and Rotavirus in Diarrhea among Children Under Five Years Old

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الخلاصة

Abstract: Ninety four fecal specimens were collected from children diarrhea less than five years old from Al-Emamain Al-Kadhemain Medical City Hospital in Baghdad province. Samples collection was carried out from 25 May to 16 July 2014. Our study showed 25.53 % and 17% of samples had positive tests for Escherichia coli and Rota virus respectively. Low number of E. coli isolates, 4/24 (16.6%) were produced β-lactamase and 10/24 (41.6%) produced biofilm. However, half E. coli isolates (50%) produced hemolysis. The E. coli isolates showed different degrees of sensitivity to different antibiotics. All E. coli isolates were 100% sensitive to Ciprofloxacin, Gentamycin, and Norfloxocin. However, 16.6% of E. coli isolates were sensitive for Carbenicillin and Amikacin. But 33.3% of E. coli isolates were sensitive to Amoxacillin- clavulanic and Aztreonam. Our study showed a number of plasmid range between 750 to 10,000 bp in size.

الكلمات الدلالية

E. coli --- Rota virus --- antibiotic sensitivity --- plasmid.


Article
Detection of Anti-Lysozomal Antibodies and Beta-2-Microglobulin Protein in Patients with Typhoid Fever

المؤلفون: Thuraya Aamer Habeeb1 , Fatima Abood Chaloob1
الصفحات: 93-98
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الخلاصة

Abstract:Infection with many microbial agents can cause aberration in immune response through molecular mimicry or prolonged stimulation of immune system. Salmonella, the causative agent of typhoid fever, may have this feature. This study aimed to explore the possible association of serum levels of anti-lysozymal IgG antibodies (ALIA) and beta-2 microglobulin (B2M) with typhoid fever. A total of 52 typhoid fever patients and 35 healthy controls were recruited for this study. From each participant, a blood sample was obtained from which serum was separated. Enzyme linked immunosorbent assay was used to estimate serum levels of anti-lysozymal IgG antibodies and B2M. Patients with typhoid fever had higher significantly serum levels of ALIA than control (5.136±3.345 U/ml and 2.946±3.257 respectively), while there was no significant difference in serum levels of B2M between the two groups (0.889± 0.766 µg/ml and 1.173±0.81 µg/ml respectively). These results indicate that infection with Salmonella can cause increase in serum level of anti-lysozymal IgG antibodies but not beta-2 microglobulin.

الكلمات الدلالية

typhoid fever --- lysozyme --- microglobulin --- Salmonella --- ELISA


Article
The Effect of Fixed Space Maintainer Appliances on Salivary Level of Immunoglobulin A and other Saliva Properties

المؤلفون: Esra Hassan Abd Ali1 , Maha Abdul- Kareem Mahmood1
الصفحات: 99-106
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الخلاصة

Abstract: Secretary immunoglobulin A SIgA , play an important role in mucosal immunity, and the salivary level change in response to infection ,social , psychological and physical stress, also the change in level of salivary IgA and other saliva properties include salivary pH, salivary flow rate SFR, and body mass index BMI in children with fixed appliances were measured .The aim of this work was to compare the values of SIgA, PH, SFR and BMI in children with and without fixed appliances. This work included 20 children (10 males and 10 females) with mean age 6 years old who were users of fixed space maintainer for at least 12 weeks, and 20 children (12 males and 8 females) without fixed appliances and with mean age = 6.7 years. The level of SIgA in saliva were assessed by ELISA test. The results revealed that the mean values of SIgA and SFR were higher in fixed appliance group than the group without fixed appliance, with statistical significant difference, but no statistical significant differences were reported for salivary pH and BMI between the studied groups. The results also showed a weak positive correlation between SIgA and SFR (r = 0.07) while negative correlation between SIgA and pH in fixed appliance children (r = 0.1). In conclusion, children with fixed appliances associated with high level of SIgA and SFR and the level of salivary pH and BMI do not correlated with fixed appliance.

الكلمات الدلالية

Secretary immunoglobulin A --- fixed appliances --- body mass --- flow rate.

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