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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2015 المجلد: 14 العدد: 2

Article
Monitoring of Microbial Pools Water Pollution Using Bioluminescence Assay

المؤلفون: Alaa K. M1, Hassan Kadhem Nemer2
الصفحات: 1-7
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الخلاصة

Microbial water pollution was investigated in pools lied in Baghdad city, which included (AL–Bayaa, AL-Jihad, AL-Yarmouk and AL-Raffedein). Swimming pools water was monitored using Bioluminescence assay which depends on the change of ATP amount that emitted from bacterial cells in the medium. Samples were analysed using two methods; first with traditional method (Heterotrophic Plate Count ) (HPC), second with Bioluminescence assay which featured by rapid and high accuracy. Also this assay does not need to an incubation period as in traditional method. Samples collected from pools were monitored by analyzing them during the period from the first day to the third day in which water has been renewed again after had been used by swimmers. Results showed continuous excess in the ATP amount through monitoring period and a strong relation between amounts of ATP and the number of growth colonies in the plates, the correlation coefficient was (R2=0.9).

الكلمات الدلالية

Microbial --- pools --- Bioluminescence.


Article
Detection of Enterotoxin (hblA,hblC and hblD ) Genes of Bacillus cereus Isolates from Different Food Samples Using Traditional and Molecular Methods

المؤلفون: Norrya A. Ali , Ahmed Saadoon Hussain
الصفحات: 8-20
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الخلاصة

A total of 245 samples of foods (pasteurized milk, cheese, yoghurt, meat, and coffee) collected from different local markets at Baghdad city. The samples were cultured on selective media , polymyxin B pyruvate egg yolk mannitol bromothymol blue agar (PEMBA) to isolate B.cereus. Results revealed that the isolation of fifty five B. cereus isolates distributed as 26.2 % in pasteurized milk , 22.8% in cheese, 30.2% in yoghurt, 15.6% in meat and 8% in coffee. To confirm the identification, all the fifty five B.cereus isolates were undergone to biochemical test , and grown on blood agar . PCR amplification resulted in the occurrence of each of the genes hblA, hblC and hblD in the percentages of 38.2%, 60% and 60% respectively out of the total number of isolates.


Article
Micronucleus formation and Sister Chromatid Exchange in Female Lymphocytes Associated with Recurrent Spontaneous Abortion

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الخلاصة

This study aimed to assess possible genomic instability in women with recurrent spontaneous abortion (RSA). Sixty four blood samples from women complaining with RSA and 41 normal fertile females, who had at least one or more child, 21 of them were pregnant women while 20 non-pregnant were collected. Frequency of micronuclei (MN) in Women with RSA was statistically increased (8.66+1.74) (p<0.05) compared with normal fertile women either pregnant (3.83+0.74) or non-pregnant (3.61+1.02). The difference between non-pregnant and normal pregnant groups was not significant. The results obtained from SCE analysis were used to detect DNA damage among RSA women and control. The result showed a significant increase (P<0.05) in SCE frequency in women with RSA (8.43+2.65) compared with normal control either non-pregnant (3.85+0.97) or pregnant women (3.52+0.94), with no significant differences between them. This concluded that the frequency of MN and SCE in cultured peripheral lymphocytes can be used as a biomarker of genomic instability in somatic cells.

الكلمات الدلالية

micronucleus --- sister chromatid exchanges --- recurrent abortion.


Article
Serum Levels of Prolactin and Complement Components (C3 and C4) in Women Infected with Trichomonas vaginalis

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الخلاصة

Trichomonas vaginalis (TV) is a sexually transmitted anaerobic protozoan parasite. In women, the infection frequently associated with infertility. However, the exact effect of the parasite that leads to infertility is a debate issue. This study aimed to determine the impact of TV infection on serum levels of prolactin (PRL), C3 and C4 and the correlation of these parameters with the infertility. A total of 142 women were investigated for TV infection and infertility. Serum levels of PRL, and complement (C3 and C4) were estimated using mini Vidas and single radial immune diffusion assay respectively. Infertile women positive for TV had significantly higher serum levels of PRL than infertile women negative for TV, while serum levels of C3 decreased significantly in both fertile and infertile TV-infected women compared with their counterparts of non-infected women. Thus, it can be concluded that increased serum levels of PRL could be incriminated as a cause of infertility in TV-infected women.

الكلمات الدلالية

Trichomonas vaginalis --- prolactin --- complement --- women infertility.


Article
Gene Number and Heredity of Yeild and Yeild Component of Maize

المؤلفون: Nadhum Y. Abed
الصفحات: 37-46
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الخلاصة

A field experiment was conducted at the field of Crop Science Department, College of Agriculture – University of Baghdad during spring and fall seasons 2009-2010. Two maize inbreds; Nz25 and B73 were planted to produce single crosses hybrid, F2, F3, BC1, BC2, BC3 and BC4. The 9 genotypes were compared using RCBD with three replicates. The hybrid vigor for the single crosses and inbreeding depression for F2 , F3 ,BC1 and BC4,the variances of environment ,additive and dominance and the degree of dominance and heritability, number of genes were estimated ,for number of ears.plant-1(NE.P-1), number of row.ear-1(NRE ),number of grain.row-1(NGR), weight for 300 grain (gm)(GW), number grain.ear-1(NGE )and grain yield.plant-1(GY) . The experimental results showed significant differences in all characters. In this study, the highest hybrid vigor especially in GY.P-1 of 161.53%. There was a decline in the characters in F2 , F3 , BC1 and BC2 so that we cannot recommend to use seeds for commercial production. The dominance genetic variance was significantly superior in all characters in this study than the additive, which indicate that the genetic variance is very important in influencing the studied traits .The estimations of the average of degree of dominance were more than one for all the studied characters. The broad sense heritability was high which ranged from 0.656 in NE.P-1 to 0.978 for GW, while there is a decline in narrow sense heritability which indicates an important dominance genetic superiority in the studied characters. The number of genes which control NE.P-1 initiation was accede 5 pairs and 4 for the NKR.E-1 imitation 14 for NK.R-1 and 18 for theNK.E-1 and16 pairs for the GW and more than 34 pairs for the GY.P-1, all these results which indicate that these characters should be improved by crossing followed by selection , for they are quantitative traits.


Article
Study of Phylogenetic Tree and Morphology of Aporrectodea Based on Mitochondrial Marker (16S rRNA gene) in Some Area South of Baghdad/ Iraq

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الخلاصة

This study aimed to show the phylogenetic structure of Aporrectodea genus in order to verify its cladistics nature and its taxonomic validity. In this work, collection of Aporrectodea genus from three locations from South of Baghdad, (AL-Karrada, AL-Zafranya and New Baghdad) are studied. First, we used usual morphological characteristics to identify each species than molecular phylogenetic analyses are based on the sequences of mitochondrial 16S rRNA gene regions and used software MEGA6 and Raptorx software. Rresults of the two methods (MEGA 6 and Raptorx software) were cluster groups (organisms of 8 sample from Group1A and Group3) in one group and with distance equal to 0.006, clustering of group 2 as a single group, and reached the highest value between group 2 and group 1(B) with distance equal to 0.272 and to move away genetic traits, Raptorx software, conformation of protein for 16SrRNA appeared as a result of the similarity of Mega6. The marker mitochondrial 16S rRNA gene is a powerful tool for identifying species of earthworms and provides a useful complement to traditional morphological taxonomy.

الكلمات الدلالية

16S rRNA --- Aporrectodea --- Raptorx software.


Article
Production, Characterization and Antimicrobial Activity of a Bioemulsifier Produced by Acinetobacter baumanii AC5 Utilizing Edible Oils

المؤلفون: Nadhem H. Hyder
الصفحات: 55-70
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الخلاصة

Biosurfactant or Bioemulsifier amphiphilic compounds are; produced by microorganisms as primary or secondary metabolites. The unique properties of biosurfactants mean that they have the potential to supplement, or even replace, chemical surfactants used in food, in the cosmetics and pharmaceutical industries; and in the environment. In the present study ten Acinetobacter sp. isolated from different sources were tested for their capability to produce bioemulsifiers in mineral salt medium with addition of 1% (v/v) edible oil as the sole source of carbon. Out of the ten Acinetobacter isolates tested, five showed lipase activity and produced bioemulsifiers exhibiting an emulsification index (EI24%) of 40 – 78%. The results revealed that the isolate Acinetobacter baumanii AC5, a gram negative, oxidase negative, aerobic and a diplococcoid rod bacterium was the best bioemulsifier producer. Optimization studies indicated that bioemulsifier production was associated with bacterial growth, and that the presence of inducer edible oils in the medium also enhanced bioemulsifier production. On the other hand, bioemulsifier production decreased when hydrocarbon (gasoline and diesel oil) were used. Crude bioemulsifier was recovered from the culture supernatant by a solvent system of chloroform: methanol (2:1 v/v), with the extraction producing 5.05 g/l of crude bioemulsifier. Partial purification and chemical analysis of the bioemulsifier revealed that it is a lipoglycan in nature with lipid content of 63%, carbohydrate 35% and a minor fraction of protein 2%. The crude bioemulsifier; showed strong antimicrobial and antifungal activity against tested pathogenic microorganisms.

الكلمات الدلالية

Acinetobacter --- Bioemulsifier --- Lipases --- Oils --- Lipoglycan --- Antimicrobial.


Article
Molecular Study of Azithromycin-Resistant P. aeruginosa

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الخلاصة

The aim of the present study was to investigate the occurrence of azithromycin resistance among P. aeruginosa swabs isolates. Out of 60 pus, burn and wound swabs, 16 (26.6%) P. aeruginosa were isolated. The susceptibilities of the P. aeruginosa isolates to the macrolides azithromycin, chloramphenicol and aminoglycosides streptomycin, gentamicin and kanamycin were evaluated by disc diffusion experiments. The azithromycin was observed to be less resistance with high susceptibilities rate 13 (81.25) as compared to aminoglycosides and chloramphenicol. Partial 23S rDNA gene sequences of 12 isolates demonstrate new single base substitution in the resistant isolates in positions A1807G, C1808A, A1823G and A1819G which confer the azithromycin resistance with low frequency rate 0.9 for each one. As we recommend the azithromycin is the drug of choice in P. aeruginosa treatment, further study is needed to whole 23S rDNA gene in local isolates to identify mutations outside the partial selected region.

الكلمات الدلالية

Azithromycin --- 23Srrna --- P. aeruginosa.


Article
Association of t (14; 18) Chromosomal Translocation to Hodgkin’s Lymphoma in Iraqi Patients Using Multiplex PCR Technique

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الخلاصة

Translocation t (14; 18) (q32;q21) is a common cytogenetic abnormality in B- cell lymphoma, especially in non-Hodgkin’s lymphoma, but it is rarely seen in Hodgkin’s lymphoma (HL). In the current study, t(14; 18) chromosomal translocation was detected in Hodgkin’s lymphoma of Iraqi patients by multiplex PCR with multiple primers and direct sequence by Sanger method with Agencourt® CleanSEQ® dye-terminator Removal Kit. DNA was extracted from 50 samples of paraffin- embedded tissue, 25 samples of blood by using QIAamp DNA Mini Kit (Qiagen /Germany) and 10 samples from reactive hyperplasia as control. The t (14, 18) was detected in 26(35%) cases that showed IGH/BCL2 gene rearrangement with a significant difference compared to control (P<0.01) or hyperplasia. The detected breakpoints represent as 5(19.2%) for major breakpoint MBR1, 4(15.38%) for MBR2 and 3(11.5%); 2(8%); 7(27%) for 3ʹMBR1, 3ʹMBR2 and 3ʹMBR4 respectively. Break points of MCR1, MCR2, 5ʹMCR were detected as 4(15.38%); 1(4%) and 2(8%)) respectively. On the other hand, no breakpoint was detected for 3ʹMBR3. Thus, this study reported an increased frequency of breakpoints in MBR regions in HL patients than mcr cluster and an increase in t (14; 18) translocation in adult’s age and in male gander. In conclusion t (14;18)(q32;q21) was detected in B- cell lymphoma by multiple primers for all three breakpoint regions, the MBR, 3ʹ MBR and mcr with multiplex PCR amplification

الكلمات الدلالية

Hodgkin’s lymphoma --- t(14;18) --- IGH/BCL2 --- MBR.


Article
Detection of Periplasmic Nitrate Reductase Enzyme in Proteolytic Pseudomonas aeruginosa

المؤلفون: Khawlah Jebur Khalaf
الصفحات: 91-98
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الخلاصة

A total of 50 P. aeruginosa isolates are leading reasons of hospital acquired infections (burns) were collected from AL-Kindy Hospital. This study focused on the quick synthesis of silver nanoparticles by this bacteria as assay for periplasmic nitrate reductase enzyme. The silver nanoparticles were described by using Atomic Force Microscopy (AFM) and found the average size was 93.55nm. From the screening profile of 50 isolates enlisted in this study the percent of napA gene positive encoding periplasmic nitrate reductase was 100%. Results revealed that nitrogen bases sequencing for PCR product of one isolate correspond reaching up to 88% as compared with nitrogen base sequence of the napA gene of P. aeruginosa strain of National Center for Biotechnology Information (NCBI).


Article
Association Between Polycystic Ovary Syndrome and Genetic Polymorphisms of CYP 17 Gene in Iraqi Women

المؤلفون: Marwa B. Mohammed1, Salwa J. AL-Awadi2, Mahfoodha A. Omran3
الصفحات: 99-110
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الخلاصة

Polycystic ovary syndrome (PCOS), whose genetic basis is not completely well understood, it is the most common endocrine disorder in women in their reproductive years. Blood samples were collected from two groups. The first group included 61 females with PCOS and the second group included 30 normal females to detect the presence of mutation in the CYP17 gene. The two groups were genotyped and a comparison was done between them. The results showed a significant difference (p<0.05) in FSH levels in patients and normal females and there is no significant difference in the levels of Testosterone, prolactin and LH. Moreover, it showed a significant difference (p<0.05) in the levels of HDL in both patients and normal females and no significant difference in the levels of (LDL, VLDL, Triglyceride and cholesterol). Two comparisons for genotype were done: one between age and genotype and the second between BMI and genotype for each group. The results showed two types of genotype, which were a TT wild type and a heterozygote TC mutant type. Furthermore, the results showed a significant difference (p<0.05) in genotype TT and TC in the group of age less than 25 years old and no significant difference in these genotypes in age groups (25-35) and those with more than 35 years old. It is concluded from this study that this single nucleotide polymorphism in the CYP 17 gene was not associated with PCOS in Iraqi women.

الكلمات الدلالية

PCOS --- BMI --- CYP17 --- SNP --- RFLP --- genotype.


Article
Extraction, Partial Purification and Characterization of Inulinase Produced from Aspergillus niger AN20 by Solid State Fermentation

المؤلفون: Ghazi M. Aziz1, Zainab W. Abdulameer1, Ali J. R. AL-Sa'ady1
الصفحات: 111-122
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الخلاصة

Inulinase produced by Aspergillus niger AN20 was partially purified using two chromatographic techniques, ion exchange chromatography by DEAE-cellulose and gel filtration by Sephadex G-150. The two steps gave specific activity of 810 U/mg proteins, the purification fold was 8.1 and enzymatic yield was 33.5 %. Some biochemical characteristics of the partially purified inulinase were determined and the results revealed that the enzyme have a molecular weight of 42 KDa., optimum pH for inulinase activity was 4.5, and the pH of the enzyme stability was the range 4.0-8.0. The maximum activity of purified inulinase activity from A. niger AN20 was determined as 50 ᴼC, while the thermal stability ranges from 20-50 Cᵒ. The aim of this study was the extraction and purification of inulinase produced from Aspergillus niger AN20 by solid state fermentation.


Article
Molecular Diagnosis of Lactobacillus Microbiota in Some Healthy Iraqi Women

المؤلفون: Marrib N. Rasheed
الصفحات: 123-134
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الخلاصة

: Molecular diagnosis of Lactobacillus species resident in vaginal ecosystem in some healthy women of Baghdad city was investigated using 16S-23SInternal Transcribed Spacer region (ITS-1) in multiplex PCR reactions with two stages. The first stage used for grouping of Lactobacilli, followed by second stage multiplex reactions. Different PCR products derived from 35 vaginal swaps were obtained, 10 of these products were chosen randomly and subjected for sequencing. Sequences alignment and in silico diagnosis using BLAST against nr databases, showed that Lactobacillus crispatus comprised the majority (40%),followed by Lb. gasseri (30%), the rest Lb. jensenii, Lb. acidophilus were 10% for each. It was noticed that Lb. crispatus and Lb. gasseri showed a little variation which is expected due to inter individual and environment variation.

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