Table of content

Iraqi Journal of Science

المجلة العراقية للعلوم

ISSN: 00672904
Publisher: Baghdad University
Faculty: Science
Language: Arabic and English

This journal is Open Access

About

The Iraqi journal of science is a quarterly specified scientific journal issued by the Faculty of Science at the University of Baghdad. The members of the Editorial Board representing the Departments of Eight Scientific departments, college of science/ Baghdad University and its related units of research, all are professors, scientists with extensive experience and considerable skills in their field of science, as well as an advisory board in support composed of professors and scientists with great reputation in their field of science from other Colleges, universities, or research institutes. The specialty of publication includes the following fields:

1.Pure and Applied Physics.
2.Mathematical Sciences.
3.Computer Science and Information Technology.
4.Science of chemistry.
5.Bio-Science technologies.
6.Earth Sciences, geo-physics, and remote sensing.
7.Astronomy, Space Sciences, and Remote Sensing Unit.
8.Science and Research of tropical area

The visions, goals, and the mechanisms of the Iraqi Journal of Science is to publish scientific research sober in the areas of Applied and Pure Sciences and instructive Iraqi society, scientific research, scientific interest large to contribute to the development of various disciplines, which provides significant support to researchers in all scientific facilities to continue to support the development plans in Iraq.

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Contact info

Baghdad University
College of Science
Baghdad
Iraq
ijs@scbaghdad.edu.iq
07903375590
http://ijs.scbaghdad.edu.iq

Table of content: 2015 volume:56 issue:4B

Article
Physical Properties and Chemical Kinetics for the Interaction of Albumin with Amoxicillin
الخواص الفيزيائية والحركيات الكيميائية لارتباط الالبومين مع الاموكسيسيلين

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Abstract

Albumin is the most abundant protein in blood plasma, serves as a circulating depot for endogenous and exogenous (e.g. drugs) compounds due to its ligand binding properties, this work aim to get information about the binding of Amoxicillin (antibiotics) with albumin, and the influence of the solvent polarity and ionic strength on it by using UV -vis spectrophotometric measurements in phosphate buffer of pH7.4 and three different temperature (290, 300, 310) K. The UV absorption shows a change and a shift in the absorbency and a shift in albumin and amoxicillin peaks, the two changes are indicative of complex formation. The stoichiometry of the interaction were calculated by the method of continuous variations which was1:1at pH 7.4.The equilibrium constant was calculated at three different temperature and ∆G°, ∆H° and ∆S° also calculated. The kinetic studies for this interaction follows first order equation with rate constant value of 16×10-4 min-1.


Article
Spectrophotometric Determination of Vancomycin Hydrochloride (Batch and Flow-Injection) Using O-Nitroaniline as diazotized Chromogenic Reagent
تقدير الفانكومايسين هيدروكلورايد بالطرائق الطيفية )الدفعة والحقن الجرياني (باستخدام اورثو نايتروانيلين كعامل ازوتة

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Abstract

New, simple and sensitive batch and nFIA spectrophotometric methods for the determination of Vancomycin Hydrochloride in pure form and in pharmaceutical preparations were proposed, These method's were based on diazotization and coupling reaction between Vancomycin Hydrochloride and diazotized O-nitroaniline in alkaline medium to form Orange water-soluble dye that is stable and has a maximum absorbance at 465nm. Acalibration graph shows that aBeer's law is obeyed over the concentration range of 0.8-60 and 5-400 μg.mL-1 of Vancomycin Hydrochloride with detection limit's of 0.16 and 1.666 μg.mL-1 of Vancomycin Hydrochloride for batch and nFIA methods, respectively. The FIA procedure sample throughput was 80 h-1. All different chemicals and physical experimental parameters affecting on the development and stability of the colored product were carefully studied, and the proposed methods were successfully applied for the determination of Vancomycin Hydrochloride in pharmaceutical preparations.


Article
Synthesis and Spectroscopic Studies of Some transition metal Complexes with Mixed ligand of Metformin and Cysteine
تحضير ودراسات طيفية لمعقدات بعض العناصر الانتقالية مع مزيج ليكندي من الميتفورمين والسيستئين

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Abstract

Mixed ligand complexes of Cu(II), Ni(II) and Co(II) with metformin(MTF) as primary ligand and cysteine(Cys) as secondary ligand have been prepared and characterized by elemental analysis, atomic absorption, molar conductivity, magnetic susceptibility measurements, FTIR,UV-Vis ,1H-NMR and 13C-NMR spectral studies. The elemental analysis, atomic absorption data reveal the formation of [1:1:1] [M:MTF:Cys] complexes.The electronic spectra and magnetic moment measurements reveal the presence of complexes in an octahedral geometry and the molar conductivity studies of the complexes indicate their non-electrolytic nature. The infrared and NMR spectral were showed that the chelation behaviour of the ligands towards selected transition metal ions are through two imino groups of MTF and the sulphur atom and the amino nitrogen of cysteine.


Article
Study of Enteritis Morbidity Among Neonatal Goats in Iraq
دراسة مرض التهاب الأمعاء لدى مواليد الماعز في العراق

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Abstract

The study was aimed to investigate the morbidity of enteritis in goate from 2007 to 2011 by using 149 records contained veterinarian and breeding records from birth to yearling age to study factors refer to animals and that in relationship with enteritis morbidity included: breed, year, birth type, sex, birth weight, weaning weight, mother weight, season and age. General mean for enteritis incident rate was 0.94% and this not affected by birth type, sex, weaning weight, maternal weight, while significantly (p<0.05) affected by breed and birth weight and highly significant affect by year, season and age. This study indicated that local goats and shami have incidence rate received to 1.30% and 1.36% while for cross goats (local x Shami) and (Shami x cross) was 0.67% and 0.68% respectively, incidences variance among years the lower was 0.71% for year 2009 while occilated for other years between 1.11% and 1.04% for 2007 and 2011 respectively. According to twin and triplet neonatal their incidence higher than single one and were 1.02%, 1.01% and 0.97% respectively. Male and female equalities in their morbidity. The higher incidence was 1.12% for heaviest neonatal more than 4Kg and reduced for weights between 2-4Kg received to 0.94%, in the other hand neonatal with weaning weight less than 12Kg their lower morbidity percentage was 0.98%, while spread among those with weight more than 20Kg received to 1.03%, but according to mothers with weights lessthan35Kg and more than 50Kg their natal morbidity percentage were 1.01% and 1.02% and reduced to 0.98% for natal due to mother with weights between 35-50Kg. The occurrence of disease variance among different season received to 1.09%, 1.04%, 1.03, 0.86%, in spring, summer, autumn and winter respectively. This study observed that incidence rates for buckling and dueling were 0.58%, 1.33% for pre weaning and 0.67%, 1.45% for post weaning to local and Shami goats respectively. Heritability for incidence rate due to enteritis received to 0.54. Correlation coefficient between enteritis disease and each of breed and birth weight and age 0.213 (p <0.01), 0.060(non significant), 0.227 (p <0.01) respectively, correlation coefficient between breed and each of birth weight and age 0.113 (non significant) and 0.532 (p < 0.01), while correlation coefficient between birth weight and age received to 0.007 (non significant).

Keywords


Article
Study of Role for Allium Sativum L. and Melatonin in Reduce Methotrexate Side Effect on Normal Male Rabbets
دراسة عن دور الثوم والميلاتونين في اختزال التأثير الجانبي لعقار الميثوتركسيت في ذكور الأرانب السليمة

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Abstract

This study was to evaluate the role of garlic and melatonin in the reduction of the toxic effect of the drug methotrexate with concentrations 0.52 mg/ kg of body weight of rabbits on total count of white blood cells and enzymes liver functions, used in this study (90) of the male rabbits ,were randomly divided into five groups, six rabbits in each group, given all the animals normal food during the experimental period of 30 days, explained to the animals at the end of each period of experience and took blood samples to count white blood cells, measure liver function enzymes like Aspartate aminotransferase (AST), Alanine aminotransferase(ALT), Alkaline phosphatase (ALP), was reached the following conclusions: (1) The treatment of male rabbits with methotrexate reduced the rate of white blood cells, compared with a negative control and other experimental groups in the first week, but for the observed improvement in the levels of the number of white blood cells (WBCS) in the aggregates experimental treatment in addition to methotrexate, garlic and melatonin. (2)The treatment of animals by methotrexate alone showed an increase in enzyme levels (AST) ,(ALT) and (ALP) while there was a decrease in enzyme levels in treated experimental animals by methotrexate addition to both the garlic and melatonin levels with high spirits. (3) Thus, it can be said that the injection of garlic extract with dose 6 mg/kg of body weight of rabbets, hormone melatonin had a preventive role in reducing the side effects of methotrexate in the number of total white blood cells and activity of hepatic enzymes.

Keywords

Methotrexate --- Garlic --- Melatonin --- Rabbets


Article
Chromosomal Aberrations in Peripheral Blood Lymphocytes in Brick Kilns Workers
الانحرافات الكروموسومية للخلايا اللمفاويه للدم المحيطي للعاملين في معامل الطابوق

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Abstract

This study was conducted on the workers of the brick kilns at Al- Nahrawan area south-east of Baghdad city to investigate the effect of the pollutant emissions (fumes and gases) released from the burned fuel in this kiln. Blood samples were taken from group individuals of workers at this brick kilns and non-working individuals as a control. The influence of these emissions on the workers' health was measured by examining certain chromosomal aberrations among the workers. These aberrations were a formation of: chromosomal rings, dicentric chromosomes, breaks arm and acentric fragments. It has been observed that most of these aberrations were found within workers of age 40 years and above, represented by formations of averages 1.50 and 1.02 for chromosomal rings and fragments respectively, whereas the results of the group of age range 10-19 years old showed chromosomal aberration types (ring 0.05, dicentric 0.2, chromosomal break 0.15 and acentric fragment 0.05) while the control results for all chromosomal aberrations (ring 0.09 dicentric 0.07, chromosome break 0.12 and acentric fragment 0.10). An increase in levels of dicentric chromosomes, chromosome breaks and acentric fragments were observed in the group of age 40 years and above (0.25, 1.50 and 1.25) respectively. All these chromosomal aberrations showed significant differences when compared to the results of groups 10-19 years of age. The results also demonstrated a relation between smoking and serving years of workers, showing (0.27, 0.28 , 1.26 and 1.12) a significant increases in chromosomal aberrations (ring, dicentric, breaks and acentric fragments) at worker group of serving years 16-20 years (0.23, 0.25, 1.28 and 1.30) and group of 21-25 years (0.27, 0.28, 1.26 and 1.12) respectively comparing with healthy individuals of chromosomal aberrations types (ring 0.05, dicentric 0.20, chromosomal breaks 0.15 and acentric 0.05) Respectively. The aim of study is to investigate the effect of gases emission on chromosomes in brick kiln workers.


Article
Pathogenicity of Carbapenems and Third Generation Cephalosporins Resistant K. pneumoniae in Murine Urinary System
امراضية الكليبسيلا الرئوية المقاومة لمضادات الكاربابنيم والجيل الثالث سيفالوسبورين في الجهاز البولي للفئران

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Abstract

One hundred and nine clinical lactose fermenter isolates were collected from different samples (urine, stool, wound swab, blood, and sputum) , in a period from February 2014 till May 2014. All samples gathered from Alyarmok laboratories, Alkadimiya laboratories, and Baghdad teaching laboratories which are situated at Baghdad city. Fifty three (48,62%) isolates were identified as Klebsiella pneumoniae depending on microscopic characterization , conventional biochemical tests and then the identification confirmed with API 20E system . The rest of 56(51, 38%) isolates represented other bacteria.Susceptibility test was achieved to all fifty-three K. pneumoniae isolates using five antibiotic disks (Ceftazidime, Ceftriaxone, Cefotaxime, Imipenem, and Meropenem). Most of tested isolates were susceptible to Meropenem and Imipenem, 90.5% and 77.3%, respectively, and less susceptible to third generation Cephalosporin. Pathogenicity of Carbapenems and the third generation Cephalosporins resistant K. pneumoniae in murine urinary system in this study revealed Cabrapenem resistant isolates (K2 and K3) displayed severe histopathological changes compared to the third generation Cephalosporins resistant isolates (K6 and K46).


Article
Study Some Immunological Parameters in Rabbits Immunized with Cryptococcus neoformans
دراسة بعض المعايير المناعيه في الارانب الممنعة بخميره المكورات الخبيئة

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Abstract

The aims of this study were to prepare Cryptococcus neoformans killed whole cell antigen and evaluate their effects by using DTH- skin test and the differential white blood cells in local rabbits. Fourteen animals of both sexes were divided randomly into two groups. The first group (7 animals) was immunized with 1 ml of Cryptococcus neoformans killed whole cell antigen (1x 10 cells/ml), subcutaneously. A booster dose was given after 14 days of the first dose. The second group (7 animals) was considered as control group. Based on results of DTH-skin test, no significant differences (P< 0.05) were recorded between the concentrations, 15 mg/ml and 7.5 mg/ml after 24, 48 and 72 hrs., but there was a significant differences (P<0.05) between these concentrations and 7.5 mg/ml and 3.75 mg/ml and control site. There was no significant difference between all types of cells (neutrophils, lymphocytes, monocytes, and eosinophils) of immunized and control groups.


Article
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
المزروع المختبري النقي لطفيلي اللشمانيا الجلديه والاحشائية في الوسط الزرعي الخالي من مصل جنين العجل

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Abstract

Leishmania causes disease ranging from self-healing cutaneous to fatal visceral leishmaniasis (VL). Leishmaniasis is reported endemic in 88 countries, including Iraq, in which 82% in low-income countries. The diseases develop following the bite of sand flies injecting Leishmania promastigotes into skin. Promastigotes transform into amastigotes in vivo multiplying within macrophages. In this study we have investigated the ability of axenic procyclic promastigotes of cutaneous Leishmania tropica and visceral Leishmania donovani survive in M199 media with or without Fetal Bovine Serum (FBS) added. Three time incubation periods were adopted (24, 48 and 72) hours and the results showed that L. tropica was able to survive and multiply in both FBS-medium and FBS-free medium, while L. donovani showed significant decrease in surviving in the FBS-free medium at day three of follow up. Such results may indicate the difference in Leishmania species requirements in growth culture in vitro.


Article
Bioremoval of Lead and Cadmium by the Alga westillopsis prolifica
الازالة الحيوية لمعـدني الرصــاص والكادمــيوم بوسـاطة طحــلب Westiellopsis prolifica

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Abstract

Westiellopsis prlifica was exposed to 1, 2, 3, 5, 7 and 10 ppm from both lead & Cadmium, in order to measure their capacity to remove these metals from the polluted aquatic environment and to study its ability to tolerant them. The algae were grown under optimum conditions. Westiellopsis prlifica had the ability to remove the lead with percentages about 31.57, 54.42, 62.35, 61.8, 57.02 and 68.34% for the concentrations 1, 2, 3, 5, 7 and 10 ppm, respectively, but it was found that these percentages were be better in the last day of the experiment for some of the concentrations 1, 2 and 3 ppm, While the tolerant of it to lead was up to the concentration 10 ppm. Westiellopsis prlifica appears ability to remove Cadmium with percentages 42.32, 52.07, 58.18, 57.6, 62.31 and 73.2% for the concentrations 1, 2, 3, 5, 7 and 10 ppm, respectively. But it is ability to tolerant was up to the concentration 10 ppm.

Keywords

Bioremoval --- heavy metals --- Algae


Article
Evaluation of Some Immunological Markers in Patients with Behcet’s Disease
تقييم بعض المعلمات المناعية في مرضى مرض بهجت

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Abstract

The local study were selected 70 Iraqi Arab Patients (39 females and 31 males), who referred to HLA laboratory in teaching laboratories in Baghdad medical city from April 2009 – May 2010 were diagnosed with Behcete's disease, C-reactive protein concentration(CRP) was evaluated by ELISA technique, anti-nuclear antibody (ANA) and anti-neutrophil cytoplasmic antibody (ANCA) were evaluated by Indirect immunofluorescence technology. The result showed significant differences (P<0.05) of CRP concentration in patients group compared with control, negative antinuclear nuclear antibody in all cases while ANCA was positive in 8.5% of patients with type P (PANCA). These results lead to suggest that ANCA auto-antibody may have a role in triggering BD

Keywords

Behcet’s Disease --- auto-antibody --- CRP --- ANCA


Article
Effect of Plant Growth Regulators (IBA, BA, and CCC) on Some Flowering Characters of Three Hybrid Lily Cultivars of (Lilium spp. L.)
تأثير منظمات النمو (IBA و BA و CCC) في بعض الصفات الزهرية لثلاثة اصناف مهجنة من ال Lilium spp. L.

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Abstract

The present study was conducted in the nursery of Floriculture Unit, University of Baghdad in Iraq during September to December of 2013. Vernalized bulbs of hybrid lily cultivars were imported from the Netherlands. The experiments were included some hybrid lily cultivars which represent the main groups of lily (longiflorum hybrids, Asiatic hybrid and trumpet hybrids). The three lily cultivars named as Tiger (Oriental), Brunello (Asiatic) and White Heaven (Longiflorum) were sprayed two times, at five weeks after planting and at eight weeks after planting with a solution of Cycocel (100 mg /l), solution of Indole butyric acid (50 mg/l) and Benzyl adenine (50 mg /l). The results indicated that foliar spray with IBA lead to decrease in flower diameter in White heaven, increase in fresh and dry weight of bulbs and decrease the time required to flowering in Tiger Edition. BA led to increase in the time required for flowering and decrease in the flower number in Brunello, in Tiger Edition led to increase in dry weight of bulbs, while in White Heaven led to increase dry and fresh weight of bulbs with a decrease in flower diameter. While foliar spray with CCC led to increase in dry and fresh weight of bulbs and flower number in Tiger Edition, in Brunello led to increase in fresh weight of the bulbs and carbohydrate content of the bulbs ,in White Heaven led to increase in fresh and dry weight of the bulbs and carbohydrate content in bulbs.

Keywords

Lilium --- IBA --- BA --- CCC --- Flowering characters.

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