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المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

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معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2017 المجلد: 16 العدد: 1

Article
Association of Helicobacter Pylori Infection and Gastric Cancer

المؤلفون: Shurooq A. Lafta, Abdul Hussein M. ALFaisal
الصفحات: 1-9
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الخلاصة

Helicobacter pylori (H. pylori) is the most important etiologic factor for gastric cancer. It is one of the most common human pathogens, which colonizes in the mucus layer of the gastric epithelium in more than 50% of the population. The study include 78 samples of gastric cancer in addition to 42 blood samples. The results from 78 gastric cancer samples showed that gastric cancer can occur red at any age, but it increases in older ages and the incidence of H. pylori infected positive gastric cancer (HIP-GC) is higher than of H. pylori infected negative gastric cancer (HIN-GC). Male is the predominant in both groups and female affected by H. pylori more than male and the intestinal type was the predominant type.

الكلمات الدلالية

Helicobacter Pylori --- Gastric Cancer.


Article
Evaluation of Micronuclei and Other Nuclear Anomalies in Buccal Cells of some Iraqi Women with Breast Cancer

المؤلفون: Farha A. Shafi, Israa H. Hamzah, Maha M. Al-Sayyid
الصفحات: 10-15
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الخلاصة

The present study was designed to evaluate the frequencies of micronuclei and other nuclear anoma¬lies in buccal mucosa cells of Iraqi women with breast cancer. This case –control study included 20 primary diagnosis breast cancer patients. They were recruited at The Oncology Teaching Hospital, Baghdad, Iraq. From January to February 2016. As control 20 apparently healthy women were enrolled in the present study. Each participant was interviewed personally about her date of birth, marital status, habits and health state, occupation using a comprehensive questionnaire. Buccal smears were taken from all participants after asking them to rinse their mouths with tap water. The analysis of micronucleated cell and nuclear anomalies were done under a total magnification of X1000, 2000 cells per subjects ( patient and control group) were scored and the results are represented as the number of micronucleated ,binucleated and karyolysis cell per 2000 cells. The scoring of the level of the micronuclei in exfoliated cells of oral mucosa showed a significant increase (P = 0.0001) in the breast cancer patients in relation to control. The mean score of micronuclei for the breast cancer patients was (19.20 ± 1.36 ) it was twofold higher than that of healthy women (control group).The frequency of binucleated cells was higher in breast cancer patients than in control, the difference was statistically significant (P > 0.05). The number of Karyolytic cells was also elevated in breast cancer patients, but this increase did not attain a significance. Increased frequency of binucleated and micronucleated cells in the buccal mucosa of breast cancer patients shows the genomic instability may be correlated with breast cancer. The results suggest that The Buccal Micronucleus Cytome (BMCyt) assay serves as a sensitive tool for studying genomic ¬ instability in primary cancer patients.

الكلمات الدلالية

Cytome assay --- binucleated cells --- karyolysis cells.


Article
Association of Androgen Receptor Gene Polymorphisms at three SNPs and their Haplotypes with Severe Oligozoospermia Risk in Iraqi Patients

المؤلفون: Mustafa F. Khadhim, Ismail A. Abdul-Hassan
الصفحات: 16-28
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الخلاصة

Infertility in otherwise healthy males be caused by a variety of disorders. These include acquired, as well as heritable conditions. Genetic causes of male infertility are of special interest to reproductive biology, because these disorders can be passed on to the offspring, single nucleotide polymorphisms are considered as one of reason of male infertility Androgens are critical steroid hormones that determine the expression of the male phenotype. Their actions are mediated by a single androgen receptor (AR) which, upon ligand binding, translocate to the nucleus to regulate the expression of androgen-responsive genes. Mutations in androgen receptor gene may lead to a disturbance in the function of the androgen receptor which, in turn, can lead to several forms of infertility. The aim of this study was to determine the frequency of rs962458(SNP1), rs6152(SNP2) and rs2361634(SNP3)and test their associations with severe oligozoospermia risk .This study was conducted using 50 severe oligozoospermia patients and 50 apparently healthy subjects (Control). The frequency of GA genotype of SNP2 was significantly (p≤ 0.05) increased in patients versus control (10 and 0%, respectively, OR=0.561, X2=4.329),while the frequency of GG genotype of SNP2 was significantly (p≤ 0.01) decreased in patients versus control(84 and 100%, respectively, OR=0.871, X2=6.524). The results of TAA and TAG haplotypes were not significant. The frequency of TAG/TAG haplotype combination was significantly (p≤ 0.01) decreased in patients versus control(78 and 100%, respectively, OR=1.072, X2=8.35),while TAG/TAA haplotype combination frequency was significantly (p≤ 0.05)increased in patients versus control(14 and 0%,respectively, OR=0.664,X2=4.69). In conclusion, both GA genotype of rs6152A>G and TAG/TAA haplotype combination may be correlated with severe oligozoospermia risk in Iraqi patients.

الكلمات الدلالية

SNP --- Androgen receptor --- PCR --- Infertility.


Article
Detecting mRNA Expression of LUNX Gene by RT-PCR in Lung Cancer Specimens of Iraqi Patients

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الخلاصة

This study was conducted to evaluate the clinical significance of mRNA expression of lung-specific X protein (LUNX) in specimens obtained from Iraqi patients. In the present study, whole blood was isolated from 140 individuals distributed into four groups as follows; Group 1: 40 Samples from smoker patients affected by lung cancer, Group 2: 40 Samples from non-smoker patients affected by lung cancer, Group 3: 30 Samples from smokers apparently healthy individuals and Group 4: 30 Samples from nonsmokers apparently healthy individuals. The messenger RNA (mRNA) expression levels of LUNX in the peripheral blood were analyzed using reverse transcription‑polymerase chain reaction (RT‑PCR). The expressions of LUNX mRNA in these groups were 11.0 folds of that in group 1 compared with group 4, 10 folds of gene Expression in group 2 compared with group 4 and 3.0 folds of gene expression in group 3 compared with group 4, using GAPDH as Housekeeping Gene. Statistical results showed that there was a high correlation between the LUNX gene expression and lung cancer; where the correlation coefficient was 0.879 and a low correlation between LUNX gene expression and smoking; where the correlation coefficient was 0.446. The results indicate that the mRNA expression of LUNX could yield a first picture to the early detection of lung cancer in the peripheral blood and thus is of significant clinical value for the diagnosis of lung cancer.

الكلمات الدلالية

mRNA --- LUNX Gene --- RT-PCR --- Lung Cancer --- Iraq.


Article
Synthesis and Antimicrobial Screening of new 9 ,10-dihydro Anthracene-9,10-endo-α,β-succinimides Bearing Pharmacologically Active Components

المؤلفون: Ahlam M. Al-Azzawi , Ahmed S. Hassan
الصفحات: 40-52
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الخلاصة

This operation five early cyclic imides bearing biologically an effect components were Syntheside. 9,10-dihydro anthracene-9,10-endo-α,β-Succinic anhydride was synthesized then used in the synthesis of target imides. Two nitrogen containing hetero cycles namely pyridine and quinazoline are selected as biologically active components to be used in this work beside two β-lactam antibiotics namely Ampicillin and Cefotaxime in addition to active folic acid. The new cyclic imides were synthesized by two steps in the first one 9,10-dihyro anthracene-9,10-endo-α,β-Succinic anhydride was introduced in reaction with 4-amino pyridine,1-amino-quinazoline-2-one, Ampicillin and Cefotaxime producing the corresponding N-(drug) or N-(heterocycle)-9,10-dihydro anthracene-9,10-endo- (α,β)-Succinamic acid with acetic anhydride which in turn were dehydrated. In the second step via reaction involving ,acetic anhydride and sodium acetate, anhydrous below reflux conditions producing the target N-(drug) or N-(heterocycle) ,10-dihyro anthracene-(9,10)-endo-α,β-Succinimides. N-(folic acid )-9,10-dihyro (anthracene-9,10-endo-α,β)-Succinimide was synthesized via direct reaction between folic acid and 9,10-dihydro anthracene-9,10-endo-α,β-Succinic anhydride in glacial acetic acid under reflux. Results of antimicrobial activity evaluation of the newly synthesized imides showed that the new imides exhibit very high antimicrobial activity against the tested bacteria and fungi.

الكلمات الدلالية

9 --- 10-dihydro anthracene-9 --- 10-endo-α --- β-Succinic anhydride --- Ampicillin --- Cefotaxime --- cyclic imides.


Article
Molecular Diagnosis of Ornethobilharzia Turkestanicum in Maysan Province - Iraq

المؤلفون: Ismael W. Ismael, Hanaa N. Abdullah, Suzan A. Al-Azizz
الصفحات: 53-60
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الخلاصة

Ornithobilharzia turkestanicum is one of the Schistosomatidae family that causing cercarial dermatitis in humans, heavy infestations and considerable economic casualties in production of animals like; sheep, goats and cow. The present study were designed to detection of O. turkestanicum in sheep at the molecular level by using 28S rRNA gene. Worms were collected from Maysan province/ Southern Iraq. Adult worms were observed in the mesenteric veins of infected sheep and identified as O. turkestanicum by traditional methods. Adult flukes used to extraction of DNA from tissue and applied on conventional PCR program to amplification of DNA. Size of PCR product were analyzed by 1% gel agarose was 1009bp in matching with specific DNA ladder (10000bp). Confirmation of molecular diagnosis of O. turkestanicum by using sequencing technique. The sequencing of the amplified PCR products were 100% identical between the adult blood flukes and Gene Bank. The conclusion is the molecular techniques are more accurate to detection of adult O. turkestanicum in compares with ordinary methods.


Article
Isolation of Candida spp. from Women with Yeast Vaginitis and Study the Effect of Different Types of Douching on Candida spp.

المؤلفون: Alaa M. Hasan
الصفحات: 61-70
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الخلاصة

The interest of current research was to determine the effect of different types of vaginal douching (which are commercially available in pharmacies and legally prescribed by gynecologist to female patients with vaginal yeast candidiasis on Candida species). From ninety seven vaginal swab samples which were collected in the presence of specialized Gynecologist, sixty seven samples were positive, while thirty samples were negative. Candida isolates includes different species as follows, Candida albicans 46 isolates (68.65 %), C. tropicales 11 isolates (16.41 %), C. parasilopses 7 (10.44 %), and C. kyfer 3 isolates (4.47 %). The inhibitory effect (inhibition diameter) of douches was ranged from 50 mm in Gyno Baking Soda to 5 mm in Women Care, while some types of douches had no effect at all. Finally, it was concluded that despite the inhibitory effect for many types of douching on yeast isolates, such douches were cannot be used as a treatment for yeast vaginitis since they may increase the risk of yeast vaginal infection.


Article
Cloning Dictyostelium Paracaspase Protein in the Acanthamoeba Expression Plasmid

المؤلفون: Entsar J. Saheb
الصفحات: 71-79
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الخلاصة

Acanthamoeba castellanii and the slime mold Dictyostelium discoideum are members of Amoebozoa. Both microorganisms were applied as model organisms in different biological studies. A. castellanii metacaspase (Acmcp) and D. discoideum paracaspase (Ddpcp) proteins have been recently discovered. Determining the function of Acmcp and Ddpcp provides a valuable information about their role in more complex organisms. This study aims to clone the Ddpcp gene in the Acanthamoeba expression plasmids. The Ddpcp gene was inserted in a plasmids containing the TBP Promoter Binding Factor (TPBF) gene promoters from A. castellanii and enhanced green fluorescent protein (EGFP) as the reporter gene. The promoters for Acanthamoeba TPBF gene was used to drive constitutive expression of EGFP protein in stably transfected Acanthamoeba. Recombinant TPBF is able to bind DNA and activate transcription as the natural Acanthamoeba TPBF. The results showed a sucssesful constract of production of recombinant Ddpcp gene in pTPBf plasmid that will be able to use later to provide a valuable information about the function of Ddpcp in the Acanthamoeba parasite

الكلمات الدلالية

Paracaspase --- Acanthamoeba --- pTPBf-EGFP plasmid.


Article
Molecular Detection of Enterococcus Surface Protein (Esp) Gene in E.faecalis and E.faecium and their Role in Biofilm Formation

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الخلاصة

Total of (104) urine samples were collected from patients suffering from urinary tract infection with different age groups from five hospitals in Baghdad (Ibn- Albalady, Al Yarmouk, Medical city, Baghdad hospital and Al-Kandy) from the period of the beginning of September to the end of December 2015. All samples were examined by traditional methods based on cultural characteristics, biochemical test and API 20 strep. The results showed the revealed of 50 isolates to Enterococcus and this confirmed by polymerase chain reaction technique based on amplification of species specific genes. PCR were performed for E.faecalis and E.faecium in order to confirm the presence of Esp gene which coding for Enterococcus surface protein using specific primer for gene, the results showed Enterococcus contain a proportion of 54% of Esp. Biofilm production was detected in E.faecalis and E.faecium by use two methods: Congo red agar method and microtiter plate method, our results showed 22(44%) of Enterococcus isolates was strong biofilm production, 25(50%)as moderate and 3(6%) as week biofilm production. By use Congo red method, in microtiter plate method results showed that 20(40%) of bacterial isolates was detected as strong, 26(52%) as moderate and 4(8%) as week biofilm production.

الكلمات الدلالية

E.faecalis --- E.faecium --- Esp --- biofilm production.


Article
Association of the A1 allele of D2 dopamine Receptor gene Polymorphisms with Alcohol and Drug abuse among some of Iraqi Population

المؤلفون: Salwa J. AL-Awadi سلوى جابر العوادي
الصفحات: 92-100
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الخلاصة

Dopamine is known to be involved in several essential brain functions, such as locomotors behavior, cognition, motivation and neuroendocrine secretion, with its actions mediated via dopamine receptors. In particular the dopamine D2 receptors has been implicated in reward mechanisms in the brain. Dysfunction of the D2 dopamine receptors lead to aberrant substance seeking behavior. A widely studied of single nucleotide polymorphism (SNP), which so-called drd2/ANKK1 Taq1A polymorphism (rs1800497, Glu713Lys) is located ~10 kb downstream from the drd2 gene in the ankyrin repeat and kinase domain containing 1 (ANKK1) gene. The presence of A1 allele of the drd2 gene has been associated with a predisposition for addiction although there are limited data about its phenotypic expression in addiction. Methods: The case control study was designed to determine the association of D2 dopamine receptors gene polymorphism and substances abused on 115 individuals (80 control and 35 person addicted with alcohol and drug substances) were collected from Al- Hilla Prison Reform Central in Babylon. DNA was extracted from the peripheral blood of all participants, and the abovementioned single-nucleotide polymorphisms (SNPs) were genotyped by RFLP –PCR (Restriction Fragment Length Polymorphism). The results were confirmed by using sequencing technique. Results: The result of the RFLP PCR and DNA sequencing methods revealed that the TaqI A1/A1 drd2 genotype was significant associated with addiction (OR= 9.75 ; 95%, CI = 1.94-29.08, P= 0.003), among 80 control A1/A1 genotype was 2.5% (2/80), while in addicted individual was 20% (7/35) and the A1 allele frequency was related with 1.9 fold higher risk for addiction than the A2 allele (OR= 1.88;95%, CI= 1.04-3.4 , p= 0.028). In conclusion, the presence of the Taq A1 allele of the drd2 gene results in a significant increase in the risk of developing addiction.

الكلمات الدلالية

D2 dopamine receptors --- Alcohol --- drug substance --- Addiction --- drd2 gene --- A1allele.


Article
Identification of E-cadehrin Methylation in Iraqi Breast Cancer Patients

المؤلفون: Sammar F. Jaafer, Ismail H. Aziz
الصفحات: 101-105
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الخلاصة

E-cadherin (CDH1), a Ca++ dependent transmembrane glycoprotein functioning in cell to cell adhesion placed in 16q22.1, is one of the cardinal regulators of morphogenesis. Decreased levels of CDH1 expression related with the advanced stage and poorly differentiated cancers. This study involved detection of CDH1 methylation in breast tissue after surgery for breast cancer patints and control cases that involved cases of fibeoadenoma, benign breast tissue and normal breast tissue. The study revealed presence of CDH1 methylation in 93.3% of patients while the unmethylated control samples were 80%.


Article
The Effects of ERCC1 Expression Levels on the Chemo-resistance of Breast Cancer Patients Treated with Platinum based Adjuvant Chemotherapy

المؤلفون: Suhad K. Karim, Ban A. Abd. AL majeed, Mohammad I. Nader
الصفحات: 106-110
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الخلاصة

Excision repair cross-complementing 1 (ercc1) is reported to be involved in the sensitivity of cancer patients to platinum-based chemotherapy. The present study to evaluate the effects of ERCC1 expression on the chemosensitivity of platinum agents in breast cancer. ercc1 expression levels were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results demonstrated patients with low ercc1 levels had chemosensitive than those with high ercc1 levels. These results suggest that overexpression of ercc1 is correlated with platinum drug resistance in breast cancer patients .The patients with low levels of ercc1 expression demonstrate a benefit from platinum-based adjuvant chemotherapy.

الكلمات الدلالية

ercc1 --- breast cancer --- platinum chemotherapy --- qRT-PCR.

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