Table of content

journal of al-qadisiyah for pure science(quarterly)

مجلة القادسية للعلوم الصرفة (فصلية).

ISSN: 19972490
Publisher: Al-Qadisiyah University
Faculty: Science
Language: Arabic and English

This journal is Open Access

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ISSN:1997-2490, The magazine published research sober after evaluation by teachers qualified with experience in the field of their specialty, as well as the Btadhad research and refinement in both Arabic and English and supervision of print in its position as scientific as well as the allocation of a copy of each number of researchers who presented their research for publication and send a copy to the ministry and the presidency of the University and the Central Library universities and colleges as well as the magazine to pay wages to the post of resident scientists

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Table of content: 2015 volume:2 issue:20

Article
Evaluation the efficacy of two varieties of bacteria Bacillus sphaericus ,Bacillus thuringiensis var. israelensis On the control of mosquito Culex quinquefasciatus Say (Diptera:Culicidae)
تقويم كفاءة نوعي البكتريا Bacillus sphaericus و B.thuringiensis var israelensis في مكافحة بعوض Culex quinquefasciatus Say (Diptera:Culicidae)

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Abstract

Abstract: the current research evaluation the efficacy of two varietes of bacteria Bacillus sphaericus and Bacillus thuringiensis israelensis and using vital factor to struggle mosquitoe Cx. quinquefasciatus, due to the impact of concentration of bacterial suspended and secondary metabolites in the larvae of mosquito of varieties, while there was no any destruction to the role of eggs and pupae and adults. Which recorded the higher rate of destruction for the larva 93.33% when treating the larva the first instar with concentration 1×106 spore / ml of bacterial suspension of Bacillus sphaericus, while the the lower rate of destruction 66.66% concentration of 1×103 spore/ml of bacterial suspension of Bacillus thuringiensis israelensis while the highest rate of destruction 90% while treating the first instar larvae concentrating of 3×106 spore/ml after 120 hours, while the lower rate of destruction 63.33% at the concentration 3×103 spore/ml at same period, for the respect of the effect of the concentration of secondary metabolic products the highest rate of destraction for the larvae of both species of bacteria mentioned 96.66% at the concentration of 100% after 72 hours of treatment, the lower rate of destruction reach for both species 66.66% at the concentration 25% in same period . أستهدف البحث الحالي تقويم كفاءة نوعين من البكتريا Bacillus sphaericus و B. thuringiensis var.israelensis وأستعمالهما عاملاً حيوياً في مكافحة بعوض quinquefasciatus Cx.، أثرت تراكيز المعلقات البكتيرية ونواتج الأيض الثانوية في يرقات هذا النوع من البعوض، بينما لم يحدث اي هلاك لدور البيوض والعذارى والبالغات. سجلت اعلى نسبة هلاك لليرقات 93.33% عند معاملة يرقات الطور الأول بتركيز 1×610 بوغ/مل من المعلق البكتيري لبكتريا B. sphaericus ، في حين كانت أوطأ نسبة هلاك 66.66% عند تركيز 1×310 بوغ/مل ، أما عند استعمال المعلق البكتيري لبكترياB. thuringiensis فبلغت اعلى نسبة هلاك 90% عند معاملة يرقات الطور الأول بتركيز 3×610 بوغ/مل بعد 120ساعة ، في حين كانت أوطأ نسبة هلاك 63.33% عند تركيز 3×310 بوغ/مل في المدة نفسها، أما بخصوص تأثير تراكيز النواتج الأيضية الثانوية فقد بلغت أعلى نسبة هلاك لليرقات لكلا نوعي البكتريا المذكورة 96.66% عند تركيز 100%بعد 72ساعة من المعاملة أما اوطأ نسبة هلاك فبلغت لكلا النوعين 66.66% عند تركيز %25 وفي المده ذاتها.


Article
Physiological harmony of hormones related to the thyroid gland function in cretinous sheep
التناغم الفسيولوجي للهرمونات المتعلّقة بوظيفة الغدّة الدرقيّة في الأغنام القميئة

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Abstract

The present study showed that cretinous rams and ewes revealed an obvious elevation of TSH values accompanied with a declined values of both T3 and T4 from the same samples. Each of these results revealed significant differences in probability values under 0.05 (p< 0.05) when compared with samples from normal sheep of the same species. It can be concluded that cretinous sheep had a disturbance in thyroid physiology opened the hypothesis that it is primary hypothyroidism type. أظهرت هذه الدراسة أن الأكباش والنعاج القميئة أظهرت إرتفاعاً واضحاً في قيم TSH مصحوبة بقيم منخفضة لكل من هرموني T3 و T4 من نفس العيّنات. كل من هذه النتائج كشفت عن إختلاف كبير في القيم الإحتماليّة تحت 0.05 (p< 0.05) عند مقارنتها مع عيّنات من الأغنام الطبيعيّة من نفس الأنواع. إنّ الإستنتاج الواقعي لدراستنا يبيّن أنّ الأغنام القميئة عانت إضطرابات في فسيولوجيا إفراز هرمونات الغدّة الدرقيّة ممّ يسند فرضيّة أنّ هذا النوع من العلل هو قصور الإفراز الدرقاني من النوع الإبتدائي.


Article
Study the efficiency of aqueous extracts of green tea and licorice on testicular tissue and epididymye for the male rats treatment by Dimethoate
ﻰﺼﺨﻟا ﺔﺠﺴﻧأ ﻰﻠﻋ سﻮﺴﻟا قﺮﻋو ﺮﻀﺧﻷا يﺎﺸﻠﻟ ﺔﯿﺋﺎﻤﻟا تﺎﺼﻠﺨﺘﺴﻤﻟا ةءﺎﻔﻛ ﺔﺳارد* ﺖﯾﻮﺜﻤﯾاﺪﻟا ﺪﯿﺒﻤﺑ ﺔﻠﻣﺎﻌﻤﻟا ﺾﯿﺒﻟا ناذﺮﺠﻟا رﻮﻛﺬﻟ ﺦﺑاﺮﺒﻟا

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Abstract

The present study aimsat assessthe protective role of aqueous extract of green tea and aqueous extract of licorice in reducing toxicity of the pesticide Dimethoate ongenital tissues of male rats. In this experiment (25) male from adult rats were randomly assigned to 5 equal groups, Each group comprises (5) animals. Control group (C) gavage with distilled water, First group (T1) gavage pesticide Dimethoateconcentration (12, 5) mg/kg of body weight, Second group (T2) gavage Dimethoateconcentration (12.5) mg/kg and aqueous extract of green tea a concentration of 100 mg/kg of body weight, Third group (T3) gavage Dimethoateconcentration (12.5) mg/kg and aqueous extract of licorice concentration of 100 mg/kg of body weight, The fourth group (T4) gavage Dimethoateconcentration (12.5) mg/kg and aqueous extract of green tea a concentration of 100 mg/kg and aqueous extract of licorice concentration (100) mg / kg of body weight. After 60 days,genitals eradicated for the purpose of textile on study them, Histological examination showed pathological changes in the occurrence of the testis and epididymis tissues in (T1) represented by its small diameter tubular deferens and the lack of high cells lining the tail of the epididymis. Also, the number of cells established for sperm cells and spermatid and leydig cells has been reducedand cells for sperm Spermatogoniaget vacuolation and necrosis of the facility. While the use of green tea extract and licorice individually or both has (synergistic combination) positive effects in the prevention of testicular tissue and culverts from the damage caused by the toxicity of the pesticide. سمیة مبید الدایمثویت على أنسجة الأعضاء التناسلیة لذكور الجرذان. استخدام في ھذه التجربة (25) ذكرا ً من الجرذان البالغة وزعت عشوائیا إلى (5) مجامیع متساویة, ضمت كل مجموعة (5) حیوانات. جرعت مجموعة السیطرة (C) بالماء المقطر, وجرعت المجموعة الأولى(T1) بمبید الدایمثویت بتركیز (5,12) ملغم/كغم من وزن الجسم , وجرعت المجموعة الثانیة(T2) مبید الدایمثویت بتركیز (5,12) ملغم/كغم والمستخلص المائي للشاي الأخضر بتركیز (100) ملغم/كغم من وزن الجسم , وجرعت المجموعة الثالثة (T3) مبید الدایمثویت بتركیز (5,12) ملغم/كغم والمستخلص المائي لعرق السوس بتركیز (100) ملغم/كغم من وزن الجسم, وجرعت المجموعة الرابعة (T4) مبید الدایمثویت بتركیز (5,12) (100) ﺰﯿﻛﺮﺘﺑ سﻮﺴﻟا قﺮﻌﻟ ﻲﺋﺎﻤﻟا ﺺﻠﺨﺘﺴﻤﻟاو ﻢﻐﻛ/ﻢﻐﻠﻣ (100) ﺰﯿﻛﺮﺘﺑ ﺮﻀﺧﻷا يﺎﺸﻠﻟ ﻲﺋﺎﻤﻟا ﺺﻠﺨﺘﺴﻤﻟاو ﻢﻐﻛ/ﻢﻐﻠﻣ ملغم/كغم من وزن الجسم. بعد 60 یوم استؤصلت الأعضاء التناسلیة لغرض أجراء الدراسة النسجیة علیھا, أظھر الفحص النسیجي تغیرات مرضیة في نسیج الخصى والبرابخ في (T1) تمثلت بصغر أقطار النبیبات الناقلة للمني وقلة ارتفاع الخلایا المبطنة لذیل البربخ وانخفاض معدل أعداد الخلایا المنشأة للنطف والخلایا النطفیة وطلائع النطف وخلایا لایدك وحصول تفجج وتنخر الخلایا المنشاة للنطف Spermatogonia. بینما كان لاستخدام مستخلصات الشاي الأخضر وعرق ﻦﻋ ﺞﺗﺎﻨﻟا رﺮﻀﻟا ﻦﻣ ﺦﺑاﺮﺒﻟاو ﻰﺼﺨﻟا ﺔﺠﺴﻧأ ﺔﯾﺎﻗو ﻲﻓ ﺔﯿﺑﺎﺠﯾإ تاﺮﯿﺛﺄﺗ (ﺔﯾرزﺄﺘﻟا ﺔﺒﯿﻛﺮﺘﻟا)ﺎﻤھﻼﻛ وأ هﺪﺣ ﻰﻠﻋ ﻼﻛ سﻮﺴﻟا .ﺪﯿﺒﻤﻟا ﺔ


Article
Effect of different concentrations of nitrate andphosphateon Geosmin and 2-Methylisoborneol production by some species of cyanobacteria

Authors: Fo'ad M. Alkam
Pages: 14-29
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Abstract

This study is considered the first of its kind in Iraq, It deals with compounds that never had been studied in Iraq called taste and odour compounds, mostfrequentweregeosmin and 2- MIB. Two species were identified as producers for taste and odour compounds, the first was Phormidiumretzii produced geosmin and Microcoleusvaginatus produced 2-MIB which were recorded for the first time in the world as a producer. The headspace solid phase microextraction method was used to extract these two compounds from aqueous solution, which were used for the first time in Iraq. Four concentrations of nitrate and phosphate were used (97, 350, 861 and 1500 µgNO3-- N/L) and(12, 40, 400 and 870 µgPO4--P/L), for observing its effect upon the production of geosmin and 2-MIB by two cyanobacterialspecies.Themaximalproductionofgeosmin was 281.38 ng/l occurred at 861µg NO3-N/L, whereas the higher production of 2-MIB was 296 ng/l occurred at the same concentration in the late of logarithmic phase. The high concentration of nitrate (1500 µgNO3--N/L) leaded to suppressed geosmin and 2-MIB productions.Themaximalproductionofgeosmin was 132.2 ng/l occurred at 870µg PO4-P/L, whereas the higher production of 2-MIB was 167.2 ng/l occurred at the same concentration in the late logarithmic phase.


Article
The study of bacterial growth on highand low-density polyethylene modified with AlhagiGraecorum
ﺔﻓﺎﺜﻜﻟا ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗا ﻰﻠﻋ ﺔﯾﺮﮭﺠﻤﻟا ءﺎﯿﺣﻻا ﺾﻌﺒﻟ يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ( AlhagiGraecorum) لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ةرﺎﺸﻨﺑ ﻢﻌﻄﻤﻟ

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Abstract

In the present study, high and low density polyethylene with 35% of polyethylene weight contents of AlhagiGraecorumfilms were investigated for their resistance to bacterial adhesion. All tested bacteriawere brought from the microbiology laboratory in college of Veterinary in Basrah University and diagnostic . The disc diffusion method was employed for this purpose. Bacterial growth on both highdensity and low-density polyethylene– AlhagiGraecorumcomposite were reported. We compared initial adhesion and surface growth of staphylococcus aureus , streptococcus pyogens, pseudomonas spp., aerugenosa, eschrishia coli and klebciala spp. A 5 mm polymer composites disc were inoculated in the 9 cm Petri dish for 24 hours and the zone of inhibition and bacterial growing were observed and recorded. After 24 hours the bacterial growing of tested bacteria were distributed all over the control agarwith surface growth on the polymeric composites films. A dense growth with much bacterial adhesion on the surface of polyethylene-AlhagiGraecorumdiscs.According to the nature of high density polyethylene surface, density ,diffuse biodegradability we found that the bacterial adhesion to LDPE is much than that of HDPE. It was found that the AlhagiGraecorumcontent have no clear inhibition against tested bacteria where this might be due to a limitation of the agar disc diffusion method. All obtained results of bacterial growth were explained in term of physicochemical properties of the microbial and biomaterial surfaces (AlhagiGraecorum in this case which comes from plant sources and in term of the cell wall of bacteria which is having negative electrical charge and the interaction between bacteria and other material depend on specific interactions, such as electrostatic and Lifshitz–Vander Waals forces, hydrophobic interactions and a variety of specific receptor–adhesin interactions .The differences in bacterial growing radius was obvious were the (1 -17) range in millimeter was obtained for both Escherchia coliو and Streptocouuspyogenes respectively. Finally our product can be consider friendly environmental material due to it’s biodegradability specially when buried in soil ا Escherichia ,Streptocouuspyogenes, Staphylcoccusaureas ﺎﯾﺮﺘﻜﺒﻟا ﻦﻣ ﮫﺴﻤﺨﻣاﺪﺨﺘﺳا ﺚﺤﺒﻟا اﺬھ ﻲﻓ ﻢﺗ ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ ﻦﻣ صاﺮﻗا ﻰﻠﻋ يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ﺔﺳارﺪﻟ coliPseudomonas.spp.,Klebsialla spp., عراﺰﻤﻟا ﻰﻠﻋ لﻮﺼﺤﻟا ﻢﺗ ذا .ضﺮﻐﻟا اﺬﮭﻟ صﺮﻘﻟﺎﺑ رﺎﺸﺘﻧﻻا ﺔﻘﯾﺮط ﺖﻣﺪﺨﺘﺳاو لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ﻖﺋﺎﻗﺪﺑ ﻢﻌﻄﻤﻟاو ﺔﻓﺎﺜﻜﻟا ﻦﻣ ﺎھﺪﯿﻛﺄﺗ ﻢﺗو هﺮﺼﺒﻟا ﮫﻌﻣﺎﺟ يﺮﻄﯿﺒﻟا ﺐﻄﻟا ﮫﯿﻠﻛ ﻲﻓ ﺔﯾﺮﮭﺠﻤﻟا ءﺎﯿﺣﻻا ﺮﺒﺘﺨﻣ ﻦﻣ ماﺮﻛ ﮫﻐﺒﺼﻟ ﺔﺒﻟﺎﺴﻟاو ﺔﺒﺟﻮﻤﻟا ﺔﯾﺮﯿﺘﻜﺒﻟا ﮫﯿﺼﯿﺨﺸﺘﻟا ﺔﯾﻮﯿﺤﻟا تارﺎﺒﺘﺧﻻا لﻼﺧ ﺖﻨﻘﺣ .%35 ﻲھ (لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ﻖﺋﺎﻗد/ ﻦﯿﻠﯿﺛا ﻲﻟﻮﺑ) يﺮﻤﯿﻟﻮﺒﻟا ﻂﯿﻠﺨﻟا ﺐﯿﻛﺮﺗ ﻲﻓ ﮫﻠﺧاﺪﻟا ﺔﯿﻧزﻮﻟا لﻮﻗﺎﻌﻟا ﮫﺒﺴﻧ ﺖﻧﺎﻛ هﺪﻤﻟ ﻢﻠﻣ 90 ﺮﻄﻗ تاذ ﺔﯿﺟﺎﺟﺰﻟا قﺎﺒطﻹا ﻲﻓ ﺮﺘﻤﯿﻠﻣ 5 ﺮﻄﻗ تاذ ﺔﻓﺎﺜﻜﻟا ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﮫﯿﻋﻮﻨﺑ ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗا ﺔﯾﺮﯿﺘﻜﺒﻟا عراﺰﻤﻟا مﻮﻤﻌﻟ يﺮﯿﺘﻜﺑ ﻮﻤﻧ رﻮﮭظ ﺔﻠﺼﺤﺘﺴﻤﻟا ﺞﺋﺎﺘﻨﻟا ﺖﻨﯿﺑو يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ﮫﻘﻄﻨﻣ ﻞﯿﺠﺴﺗو ﮫﺒﻗاﺮﻣو ﺔﻋﺎﺳ 24 ﮫﻌﯿﺒط ﻰﻠﻋ ادﺎﻤﺘﻋا ﺞﺋﺎﺘﻨﻟا تﺮﺴﻓو ﮫﻓﺎﺜﻛ ﻰﻠﻋﻻا ﺪﻨﻋ ﮫﯿﻠﻋ ﻮھ ﺎﻤﻋ ﺊطاﻮﻟا ﻦﯿﻠﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗأ لﻮﺣ ﺔﻓﺎﺜﻜﻟﺎﺑ هزﺎﯿﺘﻣا ﻊﻣ فﺎﻀﻤﻟاو ﻒﯿﻀﻤﻟا ﺮﻤﯿﻟﻮﺒﻟا ﻦﯿﺑو ﺎﯾﺮﺘﻜﺒﻟا راﺪﺠﻟ ﺔﺒﻟﺎﺴﻟا ﺔﻨﺤﺸﻟا ﻦﯿﺑ ﻲﻜﯿﺗﺎﺘﺳوﺮﮭﻜﻟا ﻞﻋﺎﻔﺘﻟاو ﺔﯿﺋﺎﯿﻤﯿﻜﻟا ﺔﯿﺋﺎﯾﺰﯿﻔﻟا صاﻮﺨﻟا اﺬھ لﻮﺣ ﻮﻤﻨﻟا اﺬھ رﺎﻄﻗا ﺖﻨﯾﺎﺒﺗ ﺚﯿﺣ ءﺎﻤﻠﻟ ﮫھرﺎﻜﻟاﻮﮭﺒﺤﻤﻟا ﻊﯿﻣﺎﺠﻤﻟاو ﺰﻟﺎﻓرﺪﻧﺎﻓ هﻮﻗ دﻮﺟو ﺎﻀﯾاو لﻮﻗﺎﻌﻟا ﮫﺘﺒﻧ ﻖﺋﺎﻗد القرص ضمن المدى (1ملم و17 ملم ) لكل من بكتریا pyogenes , .spp.Streptocouus,coli Escherchia وعلى التوالي ویمكن القول ان ھذا النوع من البولیمرات ذات اھمیھ كبیره في مجال الحفاظ على البیئة من خلال قابلیھ التحلل .ﮫﺑﺮﺘﻟا ﻲﻓ ﺎﮭﻨﻓد ﺪﻨﻋ ﺎﺻﻮﺼﺧو ﻲﺋﺎﯿﺣﻻ

Keywords

polyethylene --- Hi density --- AlhagiGraecorum --- Disc diffusion --- bacteria --- polymer --- ﺔﻓﺎﺜﻜﻟا ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗا ﻰﻠﻋ ﺔﯾﺮﮭﺠﻤﻟا ءﺎﯿﺣﻻا ﺾﻌﺒﻟ يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ( AlhagiGraecorum) لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ةرﺎﺸﻨﺑ ﻢﻌﻄﻤﻟا 2015/1/26: لﻮﺒﻘﻟا ﺦﯾرﺎﺗ 2013/6/30 : مﻼﺘﺳﻻا ﺦﯾرﺎﺗ يدﺎﻤﺣ قازﺮﻟا ﺪﺒﻋ ﺪﯿﻤﺣ ةﺎﯿﺤﻟا مﻮﻠﻋ ﻢﺴﻗ --- ﺔﻧﺮﻘﻟا /ﺔﯿﺑﺮﺗ ﮫﯿﻠﻛ ةﺮﺼﺒﻟا ﮫﻌﻣﺎﺟ Email:hamymham@gmail.com : ﺔﺻﻼﺨﻟا Escherichia --- Streptocouuspyogenes --- Staphylcoccusaureas ﺎﯾﺮﺘﻜﺒﻟا ﻦﻣ ﮫﺴﻤﺨﻣاﺪﺨﺘﺳا ﺚﺤﺒﻟا اﺬھ ﻲﻓ ﻢﺗ ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ ﻦﻣ صاﺮﻗا ﻰﻠﻋ يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ﺔﺳارﺪﻟ coliPseudomonas.spp. --- Klebsialla spp. --- عراﺰﻤﻟا ﻰﻠﻋ لﻮﺼﺤﻟا ﻢﺗ ذا .ضﺮﻐﻟا اﺬﮭﻟ صﺮﻘﻟﺎﺑ رﺎﺸﺘﻧﻻا ﺔﻘﯾﺮط ﺖﻣﺪﺨﺘﺳاو لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ﻖﺋﺎﻗﺪﺑ ﻢﻌﻄﻤﻟاو ﺔﻓﺎﺜﻜﻟا ﻦﻣ ﺎھﺪﯿﻛﺄﺗ ﻢﺗو هﺮﺼﺒﻟا ﮫﻌﻣﺎﺟ يﺮﻄﯿﺒﻟا ﺐﻄﻟا ﮫﯿﻠﻛ ﻲﻓ ﺔﯾﺮﮭﺠﻤﻟا ءﺎﯿﺣﻻا ﺮﺒﺘﺨﻣ ﻦﻣ ماﺮﻛ ﮫﻐﺒﺼﻟ ﺔﺒﻟﺎﺴﻟاو ﺔﺒﺟﻮﻤﻟا ﺔﯾﺮﯿﺘﻜﺒﻟا ﮫﯿﺼﯿﺨﺸﺘﻟا ﺔﯾﻮﯿﺤﻟا تارﺎﺒﺘﺧﻻا لﻼﺧ ﺖﻨﻘﺣ .%35 ﻲھ (لﻮﻗﺎﻌﻟا ﺔﺘﺒﻧ ﻖﺋﺎﻗد/ ﻦﯿﻠﯿﺛا ﻲﻟﻮﺑ) يﺮﻤﯿﻟﻮﺒﻟا ﻂﯿﻠﺨﻟا ﺐﯿﻛﺮﺗ ﻲﻓ ﮫﻠﺧاﺪﻟا ﺔﯿﻧزﻮﻟا لﻮﻗﺎﻌﻟا ﮫﺒﺴﻧ ﺖﻧﺎﻛ هﺪﻤﻟ ﻢﻠﻣ 90 ﺮﻄﻗ تاذ ﺔﯿﺟﺎﺟﺰﻟا قﺎﺒطﻹا ﻲﻓ ﺮﺘﻤﯿﻠﻣ 5 ﺮﻄﻗ تاذ ﺔﻓﺎﺜﻜﻟا ﺊطاﻮﻟاو ﻲﻟﺎﻌﻟا ﮫﯿﻋﻮﻨﺑ ﻦﯿﻠﯿﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗا ﺔﯾﺮﯿﺘﻜﺒﻟا عراﺰﻤﻟا مﻮﻤﻌﻟ يﺮﯿﺘﻜﺑ ﻮﻤﻧ رﻮﮭظ ﺔﻠﺼﺤﺘﺴﻤﻟا ﺞﺋﺎﺘﻨﻟا ﺖﻨﯿﺑو يﺮﯿﺘﻜﺒﻟا ﻮﻤﻨﻟا ﮫﻘﻄﻨﻣ ﻞﯿﺠﺴﺗو ﮫﺒﻗاﺮﻣو ﺔﻋﺎﺳ 24 ﮫﻌﯿﺒط ﻰﻠﻋ ادﺎﻤﺘﻋا ﺞﺋﺎﺘﻨﻟا تﺮﺴﻓو ﮫﻓﺎﺜﻛ ﻰﻠﻋﻻا ﺪﻨﻋ ﮫﯿﻠﻋ ﻮھ ﺎﻤﻋ ﺊطاﻮﻟا ﻦﯿﻠﺛﻻا ﺮﻤﯿﻟﻮﺑ صاﺮﻗأ لﻮﺣ ﺔﻓﺎﺜﻜﻟﺎﺑ هزﺎﯿﺘﻣا ﻊﻣ فﺎﻀﻤﻟاو ﻒﯿﻀﻤﻟا ﺮﻤﯿﻟﻮﺒﻟا ﻦﯿﺑو ﺎﯾﺮﺘﻜﺒﻟا راﺪﺠﻟ ﺔﺒﻟﺎﺴﻟا ﺔﻨﺤﺸﻟا ﻦﯿﺑ ﻲﻜﯿﺗﺎﺘﺳوﺮﮭﻜﻟا ﻞﻋﺎﻔﺘﻟاو ﺔﯿﺋﺎﯿﻤﯿﻜﻟا ﺔﯿﺋﺎﯾﺰﯿﻔﻟا صاﻮﺨﻟا اﺬھ لﻮﺣ ﻮﻤﻨﻟا اﺬھ رﺎﻄﻗا ﺖﻨﯾﺎﺒﺗ ﺚﯿﺣ ءﺎﻤﻠﻟ ﮫھرﺎﻜﻟاﻮﮭﺒﺤﻤﻟا ﻊﯿﻣﺎﺠﻤﻟاو ﺰﻟﺎﻓرﺪﻧﺎﻓ هﻮﻗ دﻮﺟو ﺎﻀﯾاو لﻮﻗﺎﻌﻟا ﮫﺘﺒﻧ ﻖﺋﺎﻗد القرص ضمن المدى (1ملم و17 ملم ) لكل من بكتریا pyogenes --- .spp.Streptocouus --- coli Escherchia وعلى التوالي ویمكن القول ان ھذا النوع من البولیمرات ذات اھمیھ كبیره في مجال الحفاظ على البیئة من خلال قابلیھ التحلل .ﮫﺑﺮﺘﻟا ﻲﻓ ﺎﮭﻨﻓد ﺪﻨﻋ ﺎﺻﻮﺼﺧو ﻲﺋﺎﯿﺣﻻا ﺮﻤﯿﻟﻮﺑ --- ﺎﯾﺮﺘﻜﺑ --- صﺮﻘﻟﺎﺑ رﺎﺸﺘﻧﻻا --- لﻮﻗﺎﻌﻟا --- ﺔﻓﺎﺜﻜﻟا ﻲﻟﺎﻋ --- ﻦﯿﻠﯿﺛا ﻲﻟﻮﺑ


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Abstract

Thirty soil samples were collected from Hilla City. Thirteen actinomycetes isolates was isolated. Antifungal activity of these isolates were tested against C. albicans . Results showed that actinomycetes. 4 was given higher inhibition zone compared with other isolates. Actinomycetes. 4 isolate was selected for extraction of antifungal agent. According to cultural and biochemical tests of Actinomyces. 4 these isolates was belong to Streptomyces spp. Streptomyces spp.4 isolate was grey aerial mycelium, and yellow-green substrate mycelium , no melanin production and no diffusible pigment, able to ferment sugars, negative for catalase and H2S and positive for citrate utilization. Crude extract of Streptomyces spp.4 was active against C.albicans and A.niger with inhibition zone (22mm) against C. albicans and (16 mm) against A. niger. MIC of Streptomyces spp.4 antifungal agent showed that MIC values against C. albicans , A. niger were 22, 38 µg/ ml respectively. UV spectrum of absorption for antifungal agent showed that it have a single peak with maximum absorption (λ max) 293 nm.


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Abstract

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Abstract

The black tea from more types of hot drinks mission in Iraq in particular and beverage job in all parts of the world in general, because the tea because of the composition of chemicals have a close relationship to human health, and is the tea of hot drinks that contain antioxidants, dubbed (Flavoonoidz) which protect the arteries as these antibiotics inhibit cholesterol gathered on the inner walls of the arteries and thus maintain human health. For this reason, has been studied load microbial for tea and to make sure they are free of harmful bacteria and ensure the safety of the product depending on the specification of Iraq through microbial content knowledge and comparing them to standard microbial content and microbial tests included (The Total Count of Bacteria, Molds and Yeasts promised last resort counting total Coliform) and has also study the composition of chemical tea by estimating the metal elements trace like element(Lead, Copper, Iron, Cadmium, Zinc) for black tea available in the local Markets of the City of Baghdad and fourteen brand include (Prairies, Lions, Knock, Blable).for tea Black Phil and type (Jehan, Perfume, Apple, Mahmud, Mahboba) type of black tea filled and (Ahmed Hill, Lipton, Alkozy peach, AlkozyCurrant,Ahmed perfumed) for the type of tea bags is melting fast. Taking the test results showed the presence of Microbial numbers of bacteria, yeasts and molds in tea products, as well as showing that all these free samples of the total Coliform. The test results showed chemotherapy for a higher concentration of the Lead was (0.2263µg/g) for mark tea prairies, while appeared less concentration of the Lead was (0.0000µg/g) ofTea mark (knock, Apple, Mahmud, Mahboba).As for Cadmium element higher concentration was (0.0426μg / g) for tea sign prairies and appeared less concentration of Cadmium was (0.0009μg / g) for tea brand apple. As for the Iron element and higher concentration was (2.6117μg / g) for tea brand Lipton and less focus of iron elemental appeared (0.3868μg / g) for tea brand Mahboba. As for the element Copper higher concentration was(0.2798μg / g) for tea sign Alkozy peach and less concentration of the element Copper appeared (0.1490μg / g) for tea brand perfumed. As for Zinc the highestlevelofthe element concentration was(0.0196 µg/g)sign to Lipton tea and less focus of the element concentration was(0.0026 µg/g)sign to apple tea. یعد الشاي الاسود من النباتات التي تزرع في الكثیر من بقاع العالم لكن موطنھ الاصلي الصین یعتبر الشاي الاسود من اكثر انواع المشروبات الساخنة المھمة في العراق بصورة خاصة ومن المشروبات المھمة في كافة انحاء العالم بصورة عامة وذلك لان الشاي وبسبب تركیبتة كیمیائیة لھا علاقة وثیقة بصحة الانسان , ویحتوي مضادات الاكسدة التي یطلق علیھا اسم (الفلافونویدز) التي تحمي الشرایین اذ ان ھذة المضادات تمنع تجمع الكولسترول على الجدران الداخلیة للشرایین وبالتالي تحافظ على صحة الانسان. ولھذا السبب تمت دراسة الحمولة المیكروبیة للشاي وذلك للتاكد من خلوھا من البكتیریا الضارة وللتاكد من سلامة المنتوج بالاعتمادة على المواصفة العراقیة من خلال معرفة المحتوى المیكروبي لھا ومقارنتھا بالمحتوى المیكروبي القیاسي وتضمنت الفحوصات المیكروبیة (العد الكلي للبكتریا , وعد الاعفان والخمائر واخیر عد بكتریا القولون الكلیة ) وتمت ایضا دراسة التركیب الكیمیاوي للشاي من خلال تقدیر بعض العناصر المعدنیة النزرة مثل عنصر( الرصاص , النحاس , الحدید , الكادمیوم , الزنك) للشاي الاسود المتوفر في الاسواق المحلیة لمدینة بغداد و لااربع عشر علامة تجاریة تتضمن (البراري , الاسدین ,خبط , بلابل) للشاي اسود نوع فل و (جیھان, العطور ,تفاحة, محمود , محبوبة) للشاي الاسود نوع معبئة و( احمد بالھیل, لبتون,الكوزي بالخوخ ,الكوزي بالكشمش,احمد معطر) للشاي نوع اكیاس سریعة الذوبان . واذ اظھرت نتائج الفحص المیكروبي وجود اعداد من البكتریا والخمائر والاعفان في منتوجات الشاي وكذلك تبین خلو جمیع ھذة العینات من بكتریا القولون الكلیة. واظھرت نتائج الفحص الكیمیاوي بالنسبة لعنصر صﺎﺻﺮﻟا ﺮﺼﻨﻌﻟﺰﯿﻛﺮﺗ ﻞﻗا تﺮﮭظ ﺎﻤﻨﯿﺑﻮﯾراﺮﺒﻟا ﺔﻣﻼﻌﯾﺎﺸﻠﻟ (0.2263 µg/g) صﺎﺻﺮﻠﻟ ﺰﯿﻛﺮﺗ ﻰﻠﻋﺄﻓ صﺎﺻﺮﻟا (10-g/0009μg.0 ) للشاي علامة ( خبط , تفاحة ,محمود , محبوبة ). اما بالنسبة لعنصر الكادمیوم فأعلى تركیز لة (g/μg 0426.0) للشاي علامة البراریوظھراقل تركیز لعنصر الكادمیومكانت(g/μg 0009.0) للشاي علامة تفاحة .اما بالنسبة لعنصر الحدید فأعلى تركیز لة (g/μg 6117.2) للشاي علامة لبتونواقل تركیز لعنصر الحدیدظھرت (g/μg 3868.0) للشاي علامة محبوبة .اما بالنسبة لعنصر النحاس فأعلى تركیز لة (g/2798μg.0) للشاي علامة الكوزي بالخوخواقل تركیز للعنصر النحاس ظھرت (g/μg 1490.0) للشاي علامة العطور .اما بالنسبة لعنصر الزنك فأاعلى تركیز لة (g/μg 0196.0) للشاي علامة لبتون واقل تركیرلة (g/μg 0026.0) للشاي علامة التفاحة.


Article
Physiological harmony of hormones related to the thyroid gland function in cretinous sheep

Authors: Hassan, A-S. U.
Pages: 37-43
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Abstract

The present study showed that cretinous rams and ewes revealed an obvious elevation of TSH values accompanied with a declined values of both T3 and T4 from the same samples. Each of these results revealed significant differences in probability values under 0.05 (p< 0.05) when compared with samples from normal sheep of the same species. It can be concluded that cretinous sheep had a disturbance in thyroid physiology opened the hypothesis that it is primary hypothyroidism type.


Article
Study of Ossification Stages in Quail Embryo (Coturnex japonica)

Authors: Hashim M.A-Kareem
Pages: 44-52
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Abstract

A morphogenesis study has been made for the skeleton formation of the quail (Coturnex japonica ) which included the analytical study to make the skeletal elements and the transitional changes that happens in the two processes of cartilage formation (Chondrofication ) and bones (Ossification) during the growth of the different natural phases for the diverse bones of the body. A quail was gathered of about (100) birds and brought up at home. Then the fertilized egg was gathered from those birds in about (7-9) days, those egg were incubated by an Ukranian electronic incubator in a about ( 37,5-38 Co) and 70% of temperature and humidity respectively. The embryos were used starting from day 3 of incubation to day 16 of incubation , which is the hatching day. Embryos were extracted from the egg starting from day 3 of incubation,they were treated with alcian blue pigment that is related to paint the (non ossified) cartilaginous parts of bones, and alizarin red – s pigment that is related to paint the ossified parts of the bones as an indicator for calcium in order to recognize the ossified parts from those that didn't ossified for each bone of the skeletal system of the quail.The method that used to treat the embryos with the two pigments differed with the age progress of the embryo , also it's concentration increased with the progress of the embryos age due to the formation of the organs, skin and muscles as well as the strengthening of the cartilage and it's transformation into solid bones day after day. The successive phases for the growth of the different bones were traced with complete concentration on the evolution of the cartilage (Chondrofication) and it's transitional transformation into bone (Ossification) for each bone of the vertebral skeleton , thorax gage , and the fore and hind limbs bones.


Article
Molecular Study of Aeromonasspp. Isolated from Clinical and Environmental Samples

Authors: Safaa M. Salman
Pages: 45-55
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Abstract

Receved :7/4/2015 Accepted : 9/6/2015 and Azhar N. Hussien AL-Qadisiya University / Collegeof Education / Biology Department E-Mails: Abstract A total of 259 specimen from different clinical sources from patients of AL-Diwanyia's hospitals and environmental sources ( water) were collected. Biochemical and morphological characterization tests besides the use of API 20 E and Vitek System and Polymerase Chain Reaction (PCR)Technique which used 16S rDNA geneshowed that seventeen isolates were identified as Aeromonas spp. These Isolates distributed as : 8 isolates belong to A. hydrophila , 4 isolates belong to A. sobria , 4 isolates belong to A. caviae and one isolate belong to A. salmonicida . PCR was used for detecting virulence factors such as exotoxin genes : hemolysin (hly A) , aerolysin(aer A) and cytolytic enterotoxin (aer) , results showed that 77.7% and 62.5% of environmental and clinical isolates had hly A while 44.4 % and 50 % 54 2015 ﺔﻨﺳ 2 ﺩﺪﻌﻟﺍ 20 ﺪﻠﺠﻤﻟﺍ ﺔﻓﺮﺼﻟﺍ ﻡﻮﻠﻌﻠﻟ ﺔﻴﺳﺩﺎﻘﻟﺍ ﺔﻠﺠﻣ had aer A , aer gene just found in clinical isolates in rate 25% while didn't found in environmental isolates .The same technique (PCR)was also used for detecting resistance genes of beta - lactam antibiotic : blaCMY , blaTEM , blaCTX-M , and blaSHV genes which used in this study . The results showed that 11.76% had blaCMYgene , 5.88% had blaTEM gene and 17.64% had blaCTX-M gene of all isolates , while blaSHV gene didn't found in all the types of isolates which isolated from clinical and environmental sources .


Article
Molecular Detection of Virulence Factors Genes in Pseudomonas aeruginosa Isolated from Different Infections Cases in AlDiwaniyaHospital

Authors: Wafaa A. J. Al-Kaaby
Pages: 53-58
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Abstract

Pseudomonasaeruginosa is the most important and dangerous organism in human infections due to production of several extracellular and cell-associated virulence factors that cause severe tissue damage, the most important virulence factors including (exoenzyme S, exotoxinA and lipoprotein), these factors was encoded by (exoS, toxA and oprIgene respectively). In this study we used polymerase chain traction technique (PCR) for detection virulence factor genes producing by P.aeruginosa that isolated from wound, burn and pulmonary tract infections patient swab samples. The PCR results was show that all P. aeruginosa isolates was carried virulence factors genes with difference in prevalence between them. The burn infection P. areuginosa isolates were show high prevalence of virulence factors genes more than wound and pulmonary tract infections isolates as well as the virulence factor gene (ToxA) was show high production in most isolates. In this study we concluded that the production of virulence factors genes in P. aeruginosa is important to human infection especially (ToxA) gene and the PCR technique is very specific and fast method in detection virulence factor genes in P. areuginosa .


Article
Molecular Detection of Virulence Factors Genes in Pseudomonas aeruginosa Isolated from Different Infections Cases in Al-Diwaniya Hospital
الكشف الجزيئي عن جينات عوامل الضراوة في الزائفة الزنجارية المعزولة من حالات إصابات مختلفة في مستشفى الديوانية

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Abstract

Pseudomonas aeruginosa is the most important and dangerous organism in human infections due to production of several extracellular and cell-associated virulence factors that cause severe tissue damage, the most important virulence factors including (exoenzyme S, exotoxinA and lipoprotein), these factors was encoded by (exoS, toxA and oprI gene respectively). In this study we used polymerase chain traction technique (PCR) for detection virulence factor genes producing by P. aeruginosa that isolated from wound, burn and pulmonary tract infections patient swab samples. The PCR results was show that all P. aeruginosa isolates was carried virulence factors genes with difference in prevalence between them. The burn infection P. areuginosa isolates were show high prevalence of virulence factors genes more than wound and pulmonary tract infections isolates as well as the virulence factor gene (ToxA) was show high production in most isolates. In this study we concluded that the production of virulence factors genes in P. aeruginosa is important to human infection especially (ToxA) gene and the PCR technique is very specific and fast method in detection virulence factor genes in P. areuginosa. الزائفة الزنجارية هي الكائن الأكثر أهمية وخطورة في الإصابات البشرية نتيجةً لإنتاجها عوامل ضراوة عِدة خارج الخلية والخلايا المرتبطة التي تسبب التلف الشديد للأنسجة، ومن أهم تلك العوامل المتضمنة (exoenzyme S و exotoxinA والبروتين الدهني)، وهذه العوامل تكون مشفرة بواسطة جينات (exoS و toxA و oprI على التوالي). إستخدمت في هذه الدراسة تقنية سلسلة إنزيم البلمرة (PCR) للكشف عن جينات عوامل الضراوة المنتجة بواسطة بكتريا الزائفة الزنجارية P. aeruginosa التي عزلت بواسطة مُسحات أخذت من مرضى الجروح والحروق وإصابات المسالك الرئوية. وبينت نتائج ألـ PCR أن جميع عزلات P. aeruginosa كانت حاملة لجينات عوامل الضراوة مع وجود فرق في الإنتشار فيما بينهما. عزلات P. aeruginosa من إصابات الحروق أظهرت إرتفاع معدل الإنتشار لجينات عوامل الضراوة أكثر من الجرح وعزلات إصابات المسالك الرئوية وكذلك جين عامل الضراوة (ToxA) الذي أظهر أعلى إنتاج في غالبية العزلات. وإستنتج من هذه الدراسة أن إنتاج جينات عوامل الضراوة في P. aeruginosa هو مهم بالنسبة للإصابات البشرية خاصةً جين (ToxA), وتقنية PCR هي طريقة سريعة جداً ومحددة في الكشف عن جينات عامل الضراوة في P. aeruginosa.

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Article
Comparative anatomical study of liver in males Falco peregrinus,CoturnixcoturnixandAnascrecca

Authors: DhferahJaafarAbd Ali
Pages: 56-64
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The current study was conducted in the laboratory of histology for Graduate Studies in the Department of biology/ College of Education for women / University of Kufa . The study included, anatomical study to the liver in male thirty birds, Falco peregrinus, Coturnixcoturnix, and Anascrecca(10 birds for each type). The aims of this study were achieved to estimate anatomical charecterstic and structers in three of the birds and compare between these species The results of the current study showed that the liver of the male three types of birds are located in the middle of celomic cavity. The liver composed of two lobes, small right lobe and large left lobe in F.peregrinus and C. coturnix while the right is the biggest in A. crecca, furthermore, left lobe in C. coturnix was divided into two portion, dorsal and ventral. The color of liver was graduated from dark brown – dark red in F.peregrinus and A. crecca while light brown - dark brown in C. coturnix.And the percentage of the weight of the liver in male F.peregrinus represents the lowest rate when compared C. coturnix where significant differences showed the level (p <0.02), while the difference in the percentage when compared A. crecca was clear level (p <0.06) but did not reach the level of morale.


Article
Synthesis and Characterization New Types Antimicrobial Polymers and Study Biological Activity
تخليق وتشخيص انواع جديدة من البوليمرات المضادة للجراثيم ودراسة فعاليتها البايولوجية

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This research describes the synthesis new co-polymers which can synthesized from the reaction of (Toluen sulphonic acid , 2,4-Di chloro benzoic acid , p-hydroxy benzoic acid, Aniline, and p-Bromo phenol ) with (Hydroquinone, chloro benzoic acid, 5-Sulphosalicalic acid,and p-Bromo aniline ) respectively and formaldehyde in presence of HCl as catalyst, These polymers were identified by FT-IR spectroscopy The antibacterial,antifungal and antiyeast activities of the synthesized polymers were also screened on various bacteria ,fungal and yeast .All the prepared polymers show excellent antimicrobial activities as compared to the standard ciprofloxacin and amphotericin –B drugs. يصف هذا البحث تخليق انواع جديدة من البوليمرات المشاركة التي يمكن تخليقها من تفاعل تللوين ملامل الوللفوني , 4,2 داي - كللور ملامل البوي يل , بلارا هايدر كولي ملامل البوي يل , اننللين بلارا بر ملو ويولو مل) هايلدر كيووك, كللور ملامل - البوي يل , 5 سللفو ملامل الوالوللي , بلارا بر ملو انللين عللو التلوالي الفورمالديهايلد بوجلود ملامل الهايلد كلوري كعامل - مواعد, قد تم تشخيص هذه البوليمرات المضادة للجرالثيم بواسطة التحلي الطيفي FT-IR , ميث اظهرت جمي) البلوليمرات التلي تلم تخليقهلا وعاليلة نشلطة مضلادة للجلراثيم, البكتريلا, الفطريلات الخملاترا جميل) البلوليمرات المخلقلة اظهلرت وعاليلة جيلدة مضلادة للجراثيم مقارنة م) الويبر ولوكواسين ب انمفوتريوين كعقاقير قياسية


Article
Sensitivity of Bacteria Isolated From Patients With Urinary Tract Infection for Antibiotics

Authors: Mohammed Oudah Hamad
Pages: 65-76
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Department of Ecology , Faculity of Science , University of Kufa Mohammedhamad23@gmail.com Abstract Atotal of four hundred and fourty three samples were collected from midstream urine taken from patients suffering from urinary tract infections. 130 isolates of bacteria were isolated and identified depending on their morphological properties( cultural and microscopical) and biochemical tests. The study has declared that the ratio of separation E.coli was 51(39.2%) , P.aeruginosa 20( 15.4%) , KlebsiellaPneumoniae18( 13.8) ,Proteus mirabilis . 15( 11.5%) , S.epidermidis 11(8.4%) , Salmonella typhimurium , 7( 5.3%) and Shigelladysenteriae 6( 4.6%) ,S.aureus 2(1.5%). The antibiotics sensitivity of the isolates were tested against twenty four antibiotics, the most isolates showed high resistance. The meropenem, impinem ,Siftrixone , Cifotaxime ,amikacin and ciprofloxacin were found more effective. The minimum inhibition concentrations of isolates were higher. The combination of quinolones (ciprofloxacin and norfloxacin) with aminoglycoside (amikacin), and cephalosporins (sefitrixon) and aminoglycoside with quinolones showed active synergism effect of these mixers against multidrugs resistance Pseudomonas aeruginosa and K. pneumoniae isolates.


Article
Study the effect of Topsin (Thiophanate-Methyl)fungicide on E. coli bacteria

Authors: May Hameed Mohammad Aldehamee
Pages: 77-84
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This study aimed to know the effect of three concentration of Topsin fungicide (0.05, 0.005, 0.0005) mg/l on E. coli colony isolated from sediment of Euphrates river and find if possible half lethal LC50 from pesticide to bacteria. The results appeared that different concentrations have different effects on bacterial colonies growth which were average (137-232) colony/ml, (462-538) colony/ml and (605-657) colony/ml with percentage (17.41 -29.71)%, (60.16- 65.44)% and (71.51-83.06)% to three concentration respectively. The result pointed to the value of LC50 was 0.0099 mg/l. Also test tubes that have pesticide showed growth, which pointed to bacteria adaption for these concentration. The results pointed to a significant differentiation between growth average in probability at level (0.05) and reverse correlation coefficient between concentration and average of growth.


Article
Study the effect of Topsin (Thiophanate-Methyl) fungicide on E. coli bacteria
دراسة تأثير المبيد الفطري توبسان (Thiophanate-Methyl) على بكتريا E. coli

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This study aimed to know the effect of three concentration of Topsin fungicide (0.05, 0.005, 0.0005) mg/l on E. coli colony isolated from sediment of Euphrates river and find if possible half lethal LC50 from pesticide to bacteria. The results appeared that different concentrations have different effects on bacterial colonies growth which were average (137-232) colony/ml, (462-538) colony/ml and (605-657) colony/ml with percentage (17.41-29.71)%, (60.16-65.44)% and (71.51-83.06)% to three concentration respectively. The result pointed to the value of LC50 was 0.0099 mg/l. Also test tubes that have pesticide showed growth, which pointed to bacteria adaption for these concentration. the results pointed to a significant differentiation between growth average in probability at level (0.05) and reverse correlation coefficient between concentration and average of growth. تهدف هذه الدراسة معرفة تأثير مبيد التوبسان بثلاث تراكيز مختلفة هي ( 0.05, 0.005, 0.0005) ملغم/لتر على نمو مستعمرات بكتريا E. coli المعزولة من رواسب نهر الحلة وايجاد ان امكن التركيز نصف القاتل LC50 من هذا المبيد لهذه البكتريا. اذ اظهرت النتائج ان التراكيز المختلفة للمبيد اظهرت تأثيرات مختلفة على نمو مستعمرات البكتريا وكانت معدلاتها تتراوح ما بين (137- 232) مستعمرة/ مل و (462- 538) مستعمرة/ مل و (605- 657) مستعمرة/ مل وبنسبة مئوية تتراوح ما بين (29.71-17.41)% و(65.44-60.16)% و(83.06-71.51)% لكل من التراكيز الثلاثة على التوالي. كما اشارت النتائج ان قيمة التركيز نصف القاتل LC50 من مبيد التوبسان كان 0.0099 ملغم/لتر. اظهرت انابيب الاختبار الحاوية على المبيد والبكتريا نموا في بعضها مما قد يشير الى تكيف البكتريا مع التراكيز المضافة من المبيد. اشارت النتائج وجود فروق معنوية بين معدلات النمو عند مستوى احتمالية (0.05) ووجود معاملات ارتباط عكسية بين التراكيز ومعدلات النمو.

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Article
Study the effect of Topsin (Thiophanate-Methyl) fungicide on E. coli bacteria
دراسة تأثير المبيد الفطري توبسان (Thiophanate-Methyl) على بكتريا E. coli

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Abstract

This study aimed to know the effect of three concentration of Topsin fungicide (0.05, 0.005, 0.0005) mg/l on E. coli colony isolated from sediment of Euphrates river and find if possible half lethal LC50 from pesticide to bacteria. The results appeared that different concentrations have different effects on bacterial colonies growth which were average (137-232) colony/ml, (462-538) colony/ml and (605-657) colony/ml with percentage (17.41-29.71)%, (60.16-65.44)% and (71.51-83.06)% to three concentration respectively. The result pointed to the value of LC50 was 0.0099 mg/l. Also test tubes that have pesticide showed growth, which pointed to bacteria adaption for these concentration. the results pointed to a significant differentiation between growth average in probability at level (0.05) and reverse correlation coefficient between concentration and average of growth. تهدف هذه الدراسة معرفة تأثير مبيد التوبسان بثلاث تراكيز مختلفة هي ( 0.05, 0.005, 0.0005) ملغم/لتر على نمو مستعمرات بكتريا E. coli المعزولة من رواسب نهر الحلة وايجاد ان امكن التركيز نصف القاتل LC50 من هذا المبيد لهذه البكتريا. اذ اظهرت النتائج ان التراكيز المختلفة للمبيد اظهرت تأثيرات مختلفة على نمو مستعمرات البكتريا وكانت معدلاتها تتراوح ما بين (137- 232) مستعمرة/ مل و (462- 538) مستعمرة/ مل و (605- 657) مستعمرة/ مل وبنسبة مئوية تتراوح ما بين (29.71-17.41)% و(65.44-60.16)% و(83.06-71.51)% لكل من التراكيز الثلاثة على التوالي. كما اشارت النتائج ان قيمة التركيز نصف القاتل LC50 من مبيد التوبسان كان 0.0099 ملغم/لتر. اظهرت انابيب الاختبار الحاوية على المبيد والبكتريا نموا في بعضها مما قد يشير الى تكيف البكتريا مع التراكيز المضافة من المبيد. اشارت النتائج وجود فروق معنوية بين معدلات النمو عند مستوى احتمالية (0.05) ووجود معاملات ارتباط عكسية بين التراكيز ومعدلات النمو.

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Article
The effect ofaqueous extract of garlic frozen on the blood picture , liver and kidneys, in the rats treated with cadmium chloride

Authors: هناء عناية ماهود
Pages: 85-93
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The study include two side : First: study the effect of cadmium chloride on blood picture (RBC,WBC,Hb,PCV) and the co unter system for the oxidation of glutathione and lipid peroxidation in addition to its effect on the liver and kidneys in male rats and the second study the effect of aqueous extract of garlic frozen on the toxicity of cadmium chloride, Thirty rats male were randomly divided into three equal groups : control group (c) which were given normal tap water, group treatment (M1) which given cadmium chloride concentration (30 mg / L) in their drinking water and group treatment (M2) which were given cadmium chloride concentration (30 mg / ml) and aqueous extracts of garlic frozen concentration (250 mg / L) in their drinking water . The results showed that the effect of cadmium chloride was significant, and the results showed that the aqueous extract of garlic frozen gave significant effect on the inhibition of the toxicity of cadmium chloride user concentration of (30 mg / L) .

Keywords

cadmium chloride --- liver --- kidney --- garlic


Article
The Concentrations, Distribution and Sources of Benzo (a) Pyrene in Vegetables from Al-Diwaniya Province, Iraq.

Authors: Faiq F. Karam1
Pages: 86-91
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Polycyclic aromatic hydrocarbons (PAHs) occur as pollutants in different types of food predominantly from environmental contamination, food packaging and food processing and the levels found depend on the source of the pollution. PAHs emissions from automobile traffic and industry activities are the main sources influence the PAHs levels and profiles in vegetables and fruits grown nearby. The present study was carried out to determine the levels of Benzo (a) Pyrene compound in samples of lettuce, tomato and cabbageand use these data to further estimate the dietary exposure of population how live in Al-Diwaniya province to Benzo (a) Pyrene compound. Samples of each vegetable were collected in six different places in AlDiwaniya Province and analysed for Benzo (a) Pyrene compound. The analytical method involved saponification with methanolic KOH, liquid–liquid extraction with cyclohexane, cleanup on silica gel column and determination by gas chromatography. The maximum levels of Benzo (a) Pyrene were 9.32 µg/kg in lettuce, 2.37µg/kg in tomato, 6.81 µg/kg in cabbage.


Article
Study of Some Physical Properties and thermodynamic functions for mercabtoacetamide derivatives by using calculations (DFT-Model).
دراسة بعض الخصائص الفيزيائية والدوال الثرموداينميكية لبعض مشتقات مركبتواسيتامايد باستخدام طريقة .(DFT)

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A study of (2-mercaptoacetamide) novelty ligands were carried out theoretical study by DFT/B3LYP(3-211G)(d,p) method by using the program (Gaussian 09) . The results have shown calculation that the compound (C10H12N2O2S2) has the highest value of thermodynamic functions (E0,H0,G0,A0, CV,CP,S0) but the compound (C4H8N2O2S2) has the lowest value of these functions when The results showed that both nitrogen atoms (N1(3),N6(12,4)) had the highest negative charge in the compound (C10H12N2O2S2) which makes it a strong legend when Linked to metal and the formation of the complex. For(C6H12N2O2S2,C10H12N2O2S2, C4H8N2O2S2) molecules calculated someof physical properties ( dipole moment μ in Debye ) , orbital energies (EHOMO,ELUMO in eV),IP (in e V) , (measurement stability ΔE ) , (hardness ɳ) , (Electron Affinity EA),(χElectronegativity)and(Ѡ Electrophilicity) in eV . Also For these molecules the calculated (ΔHf 0 (in KJ/mole) by using (semi-empirical method PM3 model in MOPAC program). Calculation results have shown that the compound (C6H12N2O2S2) the lower value of the heat of formation (the more Stability) as well as the compound (C10H12N2O2S2) has the lowest value of ΔE and IP. تضمنت هذه الدراسة اعتماد برنامج كاوس (Gaussian 09 ) لاستخدام طريقة الحساب التام على وفق طريقة نظرية دالة الكثافة (DFT) . وقد أظهرت نتائج الحساب أن المركب (C10H12N2O2S2)يمتلك أعلى قيمة من قيم الدوال الثرموديناميكية G0,H0,A0,E0, S0,Cp ,CV)). كما أظهرت نتائج الحساب بان لكل من ذرات النتروجين (N1(3),N6(12)) قيمة شحنة سالبة عالية في المركب (C10H12N2O2S2) مما يجعله ليكاند قوي عند ارتباطه بالفلز وتكوين المعقد. كما تم حساب بعض الخصائص الفيزيائية عزم ثنائي القطب in Debye)μ ) , طاقة المدارات(EHOMO ELUMO ), طاقة التأين (IP in eV) , فجوة الطاقةin eV) EΔ( ,الألفة الالكترونية ,الصلابة , الكهروسالبية و الالكتروفيلية, in eV) ɳ (Ѡ, χ , EA, على التوالي وكذلك تم اعتماد برنامج MOPAC لاستخدام الطريقة الشبه تجريبية PM3 لحساب حرارة التكوين للمركبات (ΔHf 0 in kJ/mole). وقد أظهرت النتائج الحساب أن المركب (C6H12N2O2S2) اكثر استقرارا لكونه يمتلك اقل قيمة في حرارة التكوين كذلك يمتلك المركب (C10H12N2O2S2) يمتلك أقل قيمة لفرق الطاقة بين المدارات (EHOMO , ELUMO) .


Article
Effect of seed size,number of irrigation peroids and nitrogen fertilization levels on grain yield of Fenugreek ( Trigonella foenum-graecum L .) and active subastance production.

Authors: Mohammed Swadi Zgher
Pages: 94-112
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Field experment was carried out to study the effect of seed size(: small 100seeds 7.56 mm 3 and large 100seeds 20mm3),irrigation(forth levels : 3 , 6 , 9 , 12 days period) and nitrogen fertilization ( 0 , 5 , 10 , 15 kgN/d). Experment set up in Black plastic Plots(9kgs capacities). The design of the experment was RCBD in split-split sub plot arragment in three replications ,seeds were sown at stayer pore in 20 gm peatmoss at 1/9/2013, and seeding trnsfer in to plots at 1/10 , sample of soil was took befor planting for analyzed to determine physical & chemical properties ,dry seeds were taken 4 month after planting for analyzed to showed grain yield, percentage of nitrogen , carbohydrate, protien, oil, proline and trigoline alkloide. Results indicated all facters and interactions were signifcant effects and incresed grain yield, percentages of carbohydrate, and oil at interaction of large seeds size and every 3 days irrigation and 5kgN/d , while max percentages of nitrogen , protein , proline and trigoline at interaction of small seeds size and 12 days irrigation and 5kgN/d.


Article
On Minimal Actions

Authors: SattarHameedHamazah Al-Janabi
Pages: 101-112
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In this paper, we introduce and study a new type of actions namely "Minimal Actions". Thought this paper, a new concepts have been illustrated including a Minimal Group, Minimal Proper functions, Minimal Bourbaki MG-Space, Minimal Cartan MG-Space and Minimal palais MG-Space and clarified their properties.


Article
Using the tasseled cap (TCT) and principle component analysis (PCA) transform on satellite image from area of Al diwaniya city
وتحليل المكون الرئيسي لصورة فضائية لمنطقة من مدينة الديوانية استخدام تحويل

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In this paper treated the tasseled cap transformation (TCT) and the principle component analysis (PCA) applied to satellite image of land sat 7 enhanced thematic mapper plus (ETM+). The image taken on march 25,2001 that included the area between Al diwaniya center and southern of its . These techniques are similar,the analysis of the transformation images obtained shows that the results of pca and tct was proved to be a reliable and convenient methods of landsat ETM+ data .the information on vegetation and soil easier to display and understand but pca is best than tct in the resolution of information . The tasseled cap transformation gives an information on the types of the vegetations and soil and the wetness was highlight as shown as the river color . وتحليل المكون الرئيسي لصورة فضائية للقمر لاندسات7 tasseled cap في هذا البحث تم استخدام تحويل اخذت بتاريخ 25 اذار من عام 2001 تتضمن منطقة بين مركز الديوانية والجنوب منها .ان النتائج التي تم الحصول عليها لكلا التحويليين تبين انها موثوقة وملائمة لبيانات لاندسات .ان المعلومة حول الغطاء النباتي والتربة اصبحت اسهل من ناحية العرض والفهم لكن تحليل المكون الرئيسي افضل من ناحية الدقة في المعطيات . اما التقنية الثانية فلها ميزة في اعطاء المعلوة عن نوعية الغطاء النباتي والتربة وان الرطوبة تظهر اعلى كما يتبين من لون النهر .


Article
Serological Study for Toxoplasmosis in newly born babies
دراسة مصلية لداء المقوسات في الأطفال حديثي الولادة

Authors: خيري عبد الله داود
Pages: 113-124
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Abstract

Toxoplasmosis is a serious problem for this the current study was conducted on newborns in the province of Diwaniyah at the period from the beginning of November 2012 until the end April 2013 through diagnosis by Rapid test primary and then tested by Latex agglutination test and verify the existence of antibodies IgM and IgG in the serum of newborns using a test ELISA test . Samples were collected from patients coming to the Children maternity teching Hospital 50 blood samples of, were collected the results showed the rapid test recorded a positive toxoplasmosis in the easiest and quick and cheapest in the diagnosis of toxoplasmosis , where results showed the current study by the results of Rapid test IgG-IgM percentage of Toxoplasmosis in 14 %, 4 % and 36% of IgG,IgM and IgG and IgM resfectivety ,where as 14% of tested samples were given a positive result when tested by Latex agglutination test, while ELISA test was appeared only 30% IgG positive. study showed that The highest infection rate in age group ( 29-20 ) and proportions infect was ( 4 % ) of the infect and acute (16 ) for interlaced infected while the infect was chronic infection rate in the age group ( ( 39-30) and at a rate (6%) , and (8 % ) using the test latex and (20 % ) using ELISA IgG examined within the age group ( 29-20 ) . داء المقوسات يمثل مشكلة خطيرة لذلك تم إجراء الدراسة الحالية على الأطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 ولغاية نهاية شهر نيسان 2013 وذلك من خلال التشخيص االمصلي بواسطة الفحص السريع Rapid test كاختبار أولي ثم اختبار تلازن اللاتكس Latex والتحقق من وجود الكلوبينات المناعية IgM and IgG في مصل حديثي الولادة وذلك باستخدام اختبارELISA . جمعت عينات الدراسة من المرضى الوافدين إلى مستشفى الأطفال والولادة التعليمي في محافظة الديوانية تضمنت جمع 50 عينة دم حيث أجريت عليها الفحوصات المصلية أظهرت نتائج الدراسة أن الفحص السريع هو الأسهل والأسرع والأرخص في تشخيص داء المقوسات حيث بينّت نتـــائج الدراسة الحــاليـة من خــلال نتائج الفحص السريع Rapid test IgG-IgM النسبة المئوية لحالات Toxoplasmosis الإيجابية (14%) (4%) (36) لكل من الإصابات المزمنة والحادة والمتداخلة على التوالي .كما أظهرت النتائج باستعمال اختبار اللاتكس Latex النسبة المئوية لحالات Toxoplasmosis الايجابية (%14) و باستعمال فحص الادمصاص المناعي المرتبط بالإنزيمEnzyme Linked Immunosorbent assay (ELISA IgG) (30) في حين لم تسجل أي إصابة باستعمال فحص ELISA IgM .بينت الدراسة الحالية بأن نسبة الإصابة أكثر شيوعا ًضمن الفئة العمرية (29-20) وبنسب إصابة بلغت (4%) للإصابة الحادة و(16) للإصابات المتداخلة بينما الإصابة المزمنة كانت نسبة الإصابة ضمن الفئة العمرية ((39-30 وبنسبة بلغت (6%) , و(8%) باستعمال اختبار اللاتكس Latex و(20%) باستعمال فحص ELISA IgG ضمن الفئة العمرية (29-20) .

Keywords


Article
The study of mechanical properties of the polyaniline: Alcaúlan clays nanocomposite
*د ا رسة الخواص الميكانيكية ) لبولي انيلين/ طين الكاؤولين ( المتناهي في الصغر

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Intercalation composite of polyaniline and clay has been reported.The composite was prepared by in polymerization of aniline with "Alcaúlan" Clay.Were studied of the mechanical properties of polyaniline/ Alcaúlan clay. Which was prepared in Almokhtbroand accurate size equal to(≤ 250)μm. Mechanical properties are studing several variables such as the coefficient Jonk, tensile strength, elongation, fracture resistance and hardness.The results showed that the hardness of PAni/clay increase with increasing PAni/clay increase with temperature. Also fracture strength increase with increasing temperatureتمت عملية اقحام مركب مكون من البولي انيلين وطين الكاؤولين بوساطة عملية البلمرة , وقد اجريت د ا رسة الخواص الميكانيكية للبولي انيلين / طين الكاؤولين الع ا رقي من خلال اعداده بالطريقة الميكانوكيميائية وبحجم حبيبي دقيق يساوي ) 250μm ≤( , ولغرض تطوير المادة تم د ا رسة الخواص الميكانيكية لعدة متغي ا رت مثل )معامل يونك , قوة الشد, الاستطالة , الصلادة , مقاومة الكسر( , حيث اظهرت النتائج ان الصلادة ومقاومة الكسر لطين الكاؤولين والمركب تزداد بزيادة درجات الح ا ررة .


Article
A Thermodynamic Study of Adsorption of Sulfadiazine on Tween 20 and Tween 40
: دراسة ثرموديناميكية لامتزاز السلفاديازين على سطحي الميسيلين Tween40 و Tween20 .

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: This study is concerned with Thermodynamic adsorption of antibiotic (Sulfadiazine) on Tween 20 & Tween 40 surfaces. Due to the high usage of Pharmacuticals and Personal Care Products (PPCP) by human, that might led to increase the rate of water pollution specially where no effective ways for treating sewerage waters. Uv-Visible Spectrophotometric technique has been used to study the adsorption isotherms. The results obtained show that adsorption isotherms on the two surfaces were of types S2 and S3 according to Giles classification which agrees with Freundlich equation of adsorption. the isotherms refers to increasing of initial adsorbate concentration substances leads to increasing of adsorption. The study is focused on the effect of temperature between the range of (303-318)K on the adsorption of sulfadiazine and the two surfaces. The results showed that the adsorption of sulfadiazine on Tween20 & Tween40 increase with increase temperature.The effect of acidic function was conducted as well,and the results of adsorption were following: pH= 9.5 ˃ 7 ˃ 4.5 ∆H values showing adsorption is physical. تضمن موضوع البحث دراسة ثرموديناميكية لامتزاز المضاد الحيوي السلفاديازين على سطوح كل من Tween 20 و Tween 40 . ظهر في السنوات الاخيرة خطر الادوية ومستحضرات العناية الشخصية (Pharmacuticals and Personal Care Products ) ((PPCPs والتي استرعت اهتمام وقلق العلماء والرأي العام بسبب كثرة استخدامها وزيادة تلويثها للبيئة. وأكتشف التلوث ب(PPCPs) في جميع انحاء العالم, وبسبب عدم وجود وسائل لمعالجة مياه المجاري في التخلص منها فأنها وكنتيجة حتمية ستذهب الى مياه الانهار(1). وتكمن خطورة تلوث المياه ب (PPCPs) في انها قد تصل الى المستهلكين لهذه المياه او عن طريق تناولهم للأغذية المروية بها, حيث سيتعرضون لجرعات متكررة من تلك الادوية ليسوا بحاجة اليها مما قد يسبب لهم خللا في وظائف الجسم وكذلك ضررا كبيرا للحياة المائية(2),هنالك ادلة قليلة تثبت تأثير هذه الملوثات على الانسان ولكن الادلة تتزايد حيث انه حتى بكميات قليلة من هذه الملوثات فإنها يمكن ان تسبب تغيرات هرمونية وسلوكية في الاسماك والثديات حيث وجد ان ذكور الاسماك في نهرPotomac River) ) قرب العاصمة الامريكية واشنطن اصبحت تمتلك أعضاءا لتكوين البيوض(3).اظهر J. M. Park (4) وجماعته مدى فعالية Tween 20 في ازالة الفينانثرين من التربة , حيث كان تركيز الفينانثرين 200 mg/Kg واظهر Tween 20 فعالية تتراوح ما بين %89-%85 في تنقية التربة مقارنة مع سطح الكاربون المفعل. استعملت مطيافية الاشعة المرئية/فوق البنفسجية في تعيين ايزوثرمات الامتزاز, وأوضحت النتائج ان ايزوثرمات الامتزاز على السطحين كانت من نوع ( ( S3 , S2 بحسب تصنيف ( ( Giles الذي يتفق مع معادلة فرندلش ولانكمير للامتزاز, حيث اتضح من هذه الايزوثرمات زيادة كمية الامتزاز مع زيادة التركيز الابتدائي للمادة الممتزه . تمت دراسة أثر درجة الحرارة ضمن المدى (318-303) كلفن على عملية الامتزاز, وأوضحت النتائج ان كمية الامتزاز تزداد بزيادة درجة الحرارة على كلا السطحين. كما تم دراسة تأثير الدالة الحامضية في عملية الامتزاز وكانت النتائج كما يأتي : 9.5 > 7 >4.5 pH= , وتوضح قيم H∆ ان الامتزاز فيزيائي.


Article
The Effect of Ionization Cross Section on Photoinduced Absorption of Bi12SiO20 Crystal

Authors: Haider Кamil Hanoon
Pages: 137-142
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This research aims to study the effect of cross section ionization (S1) on the photoinduced absorption in the bismuth silicate oxide crystal (Bi12SiO20) after it exposure to the laser with wavelengths (532nm, 633nm) and it is assumed that the theoretical model is to explain the experimental results that will get in laboratory ,where we take different values for cross section ionization in theoretical model and we noticed how it affects on the ratio(I/I0) of transmitting intensity and incident intensity.


Article
Studying of deformation effect on energy levels in heavy nuclei region

Authors: Qaissar Abdulhussein
Pages: 143-153
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The work aims to study nuclear structure for some heavy nuclei Using the first interacting bosons model (IBM-1) and it calculated low-lying structure levels To determine the type of dynamic symmetry that those isotones belongs to it (178Yb, 180Hf, 182W, 184Os ) It has been predicted angular momentum and parity for some energy levels that have not been a definite to be a specific has been confirmed each other and find the momentum and symmetry of some of the other not previously denitrified practically

Table of content: volume:2 issue:20