جدول المحتويات

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
الجامعة: جامعة بغداد
الكلية: معهد الهندسة الوراثية والتقنيات الاحيائية
اللغة: English

This journal is Open Access

حول المجلة

تاسست المجلة العراقية للتقانات الحياتية سنة2001 وصدر اول عدد منها سنة 2002، وهي مجلة علمية محكمة نصف سنوية تصدر عن معهد الهندسة الوراثية والتقنيات الإحيائية في جامعة بغداد، متخصصة في الهندسة الوراثية والتقانات الإحيائية في مجالات علوم الحياة والبيئة والعلوم الزراعية والطب البشري وطب الأسنان والصيدلة والطب البيطري والبحوث المتخصصة بموضوع المعلوماتية الحيوية Bioinformatics)).

Loading...
معلومات الاتصال

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

جدول المحتويات السنة: 2017 المجلد: 16 العدد: 2

Article
Effect of Prolactin Gene Polymorphisms on Egg Weight of White Leghorn and Hy-line Brown Laying Hen Strains.

المؤلفون: Alan A. Al-Sheikh , Ismail H. Ismail
الصفحات: 1-9
Loading...
Loading...
الخلاصة

This study was conducted at Poultry farm and Poultry Physiology laboratory /Animal Resources Department / College of Agriculture / The University of Baghdad during a period from 26/September/2013 to 1/June/2014 to find out the association of prolactin (PRL) gene polymorphisms with egg weight of laying hens. Two strains of laying hens, White Leghorn (n=100) and Hy-line Brown (n=60) were used in this study and reared in individual cages .Blood samples were collected from a brachial vein at 41 weeks of age. The PRL gene polymorphisms were determined using PCR-RFLP technique and AluI restriction enzyme. Two alleles C and T and three genotypes wild type CC (homozygous), heterozygous CT and mutant TT (homozygous) have been got. The size of the target gene fragment was 439 bp. A significant (p < 0.05) difference was showed in the Brown strain between genotypes CC and TT, the genotype CC was higher than TT in egg weight ( EW) at 34 week of age, in White strain there was a significant (p < 0.05) differences between CC and CT, also, CC with TT genotypes in EW at 30 week of age. The T allele frequency was 0.66, whereas, C allele frequency was 0.34 for Brown strain. In White strain, the C allele frequency was 0.98, whereas T allele frequency was 0.02. The distribution percentage of polymorphism of PRL gene in Brown strain were 56.60, 37.74 and 5.66 % for CT, TT and CC genotype respectively, whereas, the distribution percentage of polymorphism of PRL gene in White strain were 97.75, 1.12 and 1.12 % for CC, CT and TT genotype respectively, and the differences among percentages was significant (p <0.01). The allele frequency and distribution percentage of polymorphism for PRL gene was measured according to Hardy-Weinberg equilibrium.

الكلمات الدلالية

Egg weight --- Laying Hens --- Polymorphism --- Prolactin gene.


Article
The Effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on Leishmania tropica Promastigotes.

Loading...
Loading...
الخلاصة

This work evaluated the effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on Leishmania tropica promastigotes in vitro. The experiment included a control and tetra-plicate of L. tropica promastigotes exposed to Nd:YAG laser in 500 pulse between each pulse 6 sec, in wavelength 1060 A°; the effect of Semiconductor laser in (5, 10, 20, 30) minute , with wavelength 532 nm; also use He-Ne laser in (5, 10 , 20 , 30 ) minute , with wavelength 6328 A°; and effect of Beta and Gamma irradiation by 137Cs isotopes , in two doses (1.105607×10-7 Gy , for 2hr, and in dose 1.650992×10-7 Gy for 3hr), cesium isotopes that give two type of decay Gamma and Beta Rays. The effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on the viability of L. tropica promastigote was determined using the colorimetric MTT assay, the number of viable cells of L. tropica was fewer than control (without exposure to laser and irradiation ). Nd:YAG laser, Semiconductor laser, He-Ne laser and Beta , Gamma irradiation was efficient in killing L. tropica promastigotes in vitro, and the remain of L. tropica after exposure to laser and irradiation was devoid flagellum and may affect the capability of infection.


Article
Prevalence of HPV Genotype in Cervical Cells Among Iraqi Patients with Abnormal Pap Smears.

Loading...
Loading...
الخلاصة

Cervical cancer is a leading cause of malignant related mortality among women specifically in developing countries. Research evidence has shown that Human Papilloma virus (HPV) is one of the most common sexually transmitted infections and the cause of the vast majority of cervical cancer cases. This study was designed to determine the prevalence of molecular 16 and 18 genotypes of HPV among Iraqi patients with abnormal Pap smear findings by utilizing endpoint real time PCR for qualitative detection.The study included 90 Pap smear specimens belonging to women complaining from gynecological problems and another ten specimens obtained from apparently healthy women. Samples were collected from two major Oncology hospitals in Baghdad during the period from April, 2015 to April, 2016. Total DNA (genomic, mitochondrial, and viral) isolated from the Pap smear samples for molecular studies was examined utilizing genomic DNA purification kits of Qiagen ( QIAamp DNA Mini Kit /Germany).Pap smear cytological examination revealed that lesions in the forms of ASCUS, LSIL, HSIL and Squamous Carcinoma were observed in 23.3. 22, 20 and 3.3% respectively. The molecular detection method (Real Time PCR) showed that 16 and 18 genotypes were demonstrated in 23% of patients with HPV infections. An effective method has been used for detection of HPV genotypes yielding higher sensitivity by using global diagnostics kit.

الكلمات الدلالية

HPV --- Gene Line Mutations --- Pap smears --- Iraqi Patients.


Article
Plasmid Profile of Locally Isolated Streptococcus mutans in Relation with Mutacin Production.

المؤلفون: Mohsan H. Risan , Hameed M. Jasim, Aya R. Salh
الصفحات: 28-37
Loading...
Loading...
الخلاصة

The present study was aimed to isolate and identify Streptococcus mutans from dental caries, and ability of bacterial isolates for mutacin production and selecting the efficient producer isolate and studying plasmid profile for the selected isolate and its role in mutacin production. In order to isolate Streptococcus mutans, 80 swab samples were collected from dental caries of patients attends Al-Dora health center and Al-Zewiya health center in Baghdad city. From these samples 80 bacterial isolates were obtained. Results of identification depending on morphological, cultural and biochemical tests showed that ten of these isolates were belonged to Streptococcus mutans, then results of identification were confirmed by using VITEK-Π identification system. All isolates were screened to examine their ability in mutacin production. Plasmid profile of S. mutans S2 was studied to investigate its role in mutacin production. Results showed that S. mutans S2 has only one plasmid DNA band. This plasmid was not responsible for mutacin production according to the results of curing experiment by using interchalating ethidium bromide dye in a concentration of 800 μg/ml. Cured cells were still have the ability of mutacin production which indicated that this trait is chromosomally encoded.

الكلمات الدلالية

Plasmid profile --- Streptococcus mutans --- mutacin.


Article
Assessing the Knowledge, Attitude and Practices Towards Cervical Cancer Screening Among a Sample of Iraqi Female Population.

Loading...
Loading...
الخلاصة

Cervical cancer is one of the most common malignancies and the leading cause of cancer related deaths among the female population in developing countries. To assess the level of knowledge, attitude and practices related to cervical cancer risk factors and prevention among Iraqi women. The cross-sectional study included a total of 343 educated female participants who were divided into two groups. Group I comprised 203 employees from the Ministry of Higher Education and Scientific Research while Group II included 140 students from Baghdad Nursing College and the Technical Institute. Participants were asked to complete pre-coded standardized questionnaire prepared by the researchers to elicit information regarding their socio-demographic characteristics and their knowledge, attitudes and practices towards cervical cancer risk factors and its prevention. Overall the answers of the respondents were sub optimal; scored in average as "Poor" among the Employees and "Good" among the Students (32.5% and 68.6% respectively). The difference in scores between the two groups was statistically significant (p < 0.5). Only 38.4% of the employed respondents have heard about Pap smear test versus 85% of the students; the main source of information in the latter was the university curriculum, while among the employed sector the media and TV played the major role. It was displayed that 17% of the married respondents in the study were subjected to Pap smear test before; two thirds of those were examined during the past two years. Favorable positive attitudes towards screening were demonstrated in only 30.3% and 40% among the employed and students groups respectively. The main barrier to screening was fear among the employed group (44.4%), while the lack of sufficient time (60%) was a more important factor behind neglecting screening among the students. In general, only 8.5% of the married respondents believed in the significance of adhering to a routine screening test. The presented findings documented deficit knowledge, attitude and practices related to cervical cancer and Pap smear among the educated Iraqi female population. Efforts should be directed towards establishing national community-based cervical cancer control programs which comprise educational components that focus on elevating the level of awareness among Iraqi women on the risk factors of cervical cancer and the means for its prevention.

الكلمات الدلالية

Cervical Cancer --- Knowledge --- Attitude --- Practice --- Pap smear --- Iraq.


Article
Generation of STR Profile From Touched Glass Surface.

المؤلفون: Mohammed M. Al-Zubaidi, Majeed A. Sabbah
الصفحات: 48-54
Loading...
Loading...
الخلاصة

Crime scene investigation involves analysis of surfaces for criminal traces. Touch DNA analysis now one of the essential tests performed for criminal identification. The aim of this study was to investigate the possibility of generation STR profile from touched glass surface. Window glass surface was touched with clean hand for 15 seconds then a double buccal swab methods used to collect possible skin cells. DNA extraction was performed using Chelex method then quantified by real time PCR. Quantified DNA amplified by STR kit (Minifiler) then analyzed using ABI 3130XL genetic analyzer. The results showed that the extracted DNA was quantified and analyzed successfully to give intact STR profile. These results indicated the capability of generating full DNA STR profile from glass surface using Chelex method.

الكلمات الدلالية

Touch DNA --- Glass --- STR --- Minifiler --- forensic.


Article
Reliable Reference Gene for Normalization of RT-qPCR Data in Human Cancer Cell Lines
Subjected to Gene Knockdown.

المؤلفون: Inam J. Lafta
الصفحات: 55-66
Loading...
Loading...
الخلاصة

Quantitative real-time Polymerase Chain Reaction (RT-qPCR) has become a valuable molecular technique in biomedical research. The selection of suitable endogenous reference genes is necessary for normalization of target gene expression in RT-qPCR experiments. The aim of this study was to determine the suitability of each 18S rRNA and ACTB as internal control genes for normalization of RT-qPCR data in some human cell lines transfected with small interfering RNA (siRNA). Four cancer cell lines including MCF-7, T47D, MDA-MB-231 and Hela cells along with HEK293 representing an embryonic cell line were depleted of E2F6 using siRNA specific for E2F6 compared to negative control cells, which were transfected with siRNA not specific for any gene. Using RT-qPCR, Ct (threshold cycle) values of 18S and ACTB were determined in transfected cells and compared with control cells. In the selection of the above cell lines, 18S was identified as the most stably expressed reference gene than ACTB in gene knockdown experiments.

الكلمات الدلالية

Reference gene --- RT-qPCR --- human cancer cell lines --- gene knockdown --- 18S --- ACTB.


Article
The Evaluation of Serum IFN-γ and TGF-β in Some Patients with Type1 Diabetes mellitus in Al-diwanyah Province.

المؤلفون: Ghasoun M. Wadai
الصفحات: 67-73
Loading...
Loading...
الخلاصة

The diabetes mellitus type one (T1DM) is considered as autoimmune disease due to the destruction of Langerhans beta cell, which is the insulin-producing cell as a result of the invasion by cells of the immune system with vital roles. There is evidence that interferone-γ IFN-γ is responsible for the T1DM disease developing, and so the transforming growth factor type beta ( TGF-β) was known as an important regulator of the immune response. Throughout of this study gain more understanding about the two cytokines role in this disease and the correlation between them to provide immunotherapeutic strategies for T1DM. A total of 35 with T1DM (19 female, 16 male), in addition to (15) healthy control subjects undertook the measurement of serum IFN-γ and TGF-β by ELISA technique. The result explains higher mean serum levels of IFN-γ (684.867 ± 246.23 pg/ml) were observed in the investigated patients compared to healthy control (11.75 ± 4.56 pg/ml). Whereas observed decline in the serum TGF-β (302.2± 65.43 pg/ml) as compared to healthy control (1153 ± 186.35 pg/ml) at the level (p<0.01), and so the result explained a significant negative correlation between TGF_β and INF_γ concentration (p<0.05). The conclusion is that T1DM patients were detected with a significantly increase in INF_γ and decrease TGF_β serum level, and they have the antagonistic effect on the T1DM development.

الكلمات الدلالية

IFN-γ --- TGF-β --- T1DM.


Article
Cytotoxicity of 1, 2-Dihydroxybenzene Towards Hep-2 and AMGM5 Cancer Cell Lines in Presence of SuperOxidase Dismutase and Peroxidase .

المؤلفون: Marwa I. Salman
الصفحات: 74-80
Loading...
Loading...
الخلاصة

The cytotoxic effect of 1,2-Dihydroxybenzene(catechol) was studied in human cancer cell lines, Epidermoid larynx carcinoma (Hep-2) and Cerebral glioblastoma multiforme (AMGM5), catechol undergo autoxidizes in physiological buffer to quinones. The results showed that catechol has a fatal effect to these cells after 72h of exposure .This toxicity was connected to the creation of quinones. There was a noticeable defeat of cell viability in a dose reliant manner in both cell type. Cytotoxicity was vetoed by the adding of 100ul SOD, while the addion of 500 ul POD or SOD combined with POD did not upturn the inhibition encouraged by SOD alone in both cells type.

الكلمات الدلالية

AMGM5 --- Hep-2 --- SOD --- 1 --- 2-dihydroxybenzen.


Article
Expression of Carbonic Anhydrase IX (CA9) in Human Tumor Tissue of Patients with Ovarian Cancer

المؤلفون: Jawdat N. Gaaib, Maisaa G. Jumaa
الصفحات: 81-88
Loading...
Loading...
الخلاصة

: The aim of the present study is to determine the expression levels of the CAIX gene in the Paraffin-embedded tissue blocks from 43 patients with different stages of newly diagnosed ovarian cancer as well as 14 tissue samples of patients with benign ovarian tumors were used as a control group. The study performed using a reverse transcription-quantitative polymerase chain reaction (RT-qPCR). According to malignancy status the percentage of patients with CAIX-positive gene expression was significantly higher in compare with benign tumor patients. Depending on the cutoff value, the present study showed that 40(93.02%) of ovarian cancer samples were CAIX positive, which is statistically significant comparing with CAIX-negative samples 3(6.97%). Statistically there was no significant difference in the levels of gene expression with age, menopausal state, and family history. In correlation with histopathologic tumor types and tumor stage, the present study showed significant associations of high level of CAIX gene expression with mucinous histopathologic tumor types( p<0.05), and stage I of ovarian cancer ( p<0. 0.05). The present study concluded that the possibility of using CAIX gene as a useful tool for discriminating malignant ovarian tumors from non-malignant ones. We also demonstrated the diagnostic value of CAIX gene for early diagnosis of ovarian cancer.

الكلمات الدلالية

carbonic anhydrase IX; CA9 ; ovarian cancer.


Article
Detection of siRNA-Directed DNA Methylation and Gene Silencing in Response to Salinity Stress

المؤلفون: Hatem K. Abbas, Mike Wilkinson, Penny Tricker
الصفحات: 89-110
Loading...
Loading...
الخلاصة

Small RNAs are able to organise a lot of biological machineries in organisms. Different types of sRNAs play divergent roles in the regulation of gene expression in plants, including RNA-directed DNA methylation (RdDM), which results from double-stranded RNAs and is often capable of directing DNA cytosine methylation at target sequences. Cytosine methylation forms the basis of an epigenetic regulatory system that has been shown to be involved in abiotic and biotic stress responses of plants. In this study applied a direct delivery method to introduce SOS1-siRNA (small interfering RNA) and HKT1-siRNA into Arabidopsis thaliana to test their ability to direct DNA methylation within promoters of the AtSOS1 and AtHKT1 genes in response to salt stress during the growth period. There was clear evidence of the siRNAs’ uptake and that the AtSOS1 promoter was methylated in the presence of HKT1-siRNA when treated with salt. In addition, lethal effects were observed on the phenotype, which suggested that AtSOS1 and AtHKT1 genes’ expressions had been affected. Direct siRNA delivery was therefore used successfully and it caused significant effects on the phenotype of plants. This method has the potential to allow targeted regulation of gene expression in response to stress.


Article
Detection of DNA Hypermethylation in Blood Samples of Breast Cancer Iraqi Patients

المؤلفون: Wiaam A. AL-Amili
الصفحات: 111-116
Loading...
Loading...
الخلاصة

In cancers, epigenetic alterations such as DNA methylation are involved in the earliest phases of tumorigenesis. Aberrant DNA methylation has been recognised as one of the most common molecular abnormalities in breast cancer and is considered a candidate biomarker of diagnosis .In this study DNA methylation was examined whether it could be correlated with breast cancer in Iraq using two classical restriction enzyme pairs HpaII – MspI. Thirty women with breast cancer (BC) Iraqi patients and 25 apparently healthy individuals were subjected to this study. Results show that 22 out of 30 (73.3%) Iraqi BC patients have DNA hypermethylation when compared with control group, which indicate that DNA hypermethylation may play an important role in breast cancer cases in Iraq. Results of this approach support the idea that DNA hypermethylation enable breast cancer prediction and using of this biomarker for prognostic and therapeutic goal. So, this research recommends for an extensive Iraqi study include larger sample size to confirm this important result.

الكلمات الدلالية

Breast cancer; DNA methylation; early detection.

جدول المحتويات السنة: المجلد: العدد: