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Basrah Journal of Veterinary Research.

مجلة البصرة للابحاث البيطرية

ISSN: Print:18138497 E; 24108456
Publisher: Basrah University
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

About

. Basrah Journal of Veterinary Research (Bas.J.Vet.Res.) is the official journal of College of Veterinary Medicine, University of Basrah, Iraq. The Journal is a biannual international peer-reviewed, open access research publication covering all aspects of veterinary science. The journal publishes original papers in English, case reports and reviews relevant to any area of veterinary sciences and veterinary medicine.

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Contact info

Mobile;07801034925
E.mail;alaasawad24@gmail.com

Table of content: 2017 volume:16 issue:1

Article
HISTOLOGICAL AND MOLECULAR STUDY OF SPLEEN IN Japanese Quail UNDER THERMAL CONDITION

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Abstract

The present study was conducted to determine the histological and Molecular changes in the spleen of adult male Japanese quail after being exposed to effect of thermal stress was recorded. This study included two groups, each group consisting twelve male birds, the control group (A) was exposed to normal temperature for 45days, while the group (B) exposed to temperature (42OC) for 45 days. The histological and molecular changes were studied during (15, 30 and 45) days of the experiment, histological changes in spleen were represented by necrosis, degeneration hemorrhage and shrinkage fibrosis of lymphocytic nodules, cytoplasmic vaculation, infiltration of lymphocytes, metamorphosis of lymphocyte, nuclei pyknotic, dilation in the central vein of lymphatic nodules, edema, dilation of sinusoids and congested blood vessels. Molecular examination showed that the heat shock protein (hsp70) gen is present in temperature group that is also found in the control group. This confirms that the hsp70 gen is present in birds at normal and abnormal conditions.


Article
WANDERING THYROID FOLLICLES WITHIN THE RENAL TISSUE OF CARP FISH "Cyprinus Carpio".

Authors: Amer M. Hussin
Pages: 11-19
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Abstract

Twenty healthy adult Carp fish were collected from Dijlah river in Iraq in Summer and winter times of the year (Ten for each ). The fish were sacrificed and fixed in 10% neutral buffered formalin solution. Routine histological preparations were carried out. Hematoxylin - Eosin stain was used. The finding of the current study showed that clear connective tissue capsule was not observed. Instead, wide loose connective tissue was surrounded the follicles. The thyroid follicles were distributed individually or in groups in the kidney and sub pharyngeal region( around the ventral aorta). The thyroid tissue was represented by thyroid follicles of different sizes and shapes. In Carp fish, the building up and organization of the follicular cells of thyroid follicles were simple and poorly organized. There was a close relationship between the morphological state of thyroid follicles and the season( temperature). The results of the present study also demonstrated that the spread of thyroid follicles in summer was more than in winter where the follicles appeared as aggregated at the periphery of renal tissue and also the results of this study showed that large and small follicles were combined. The study also registered that, during winter, the large follicles were mostly formed by the aggregation of smaller ones and that according to the physiological status of the thyroid tissue. The study concluded firstly that these follicles had the ability to wandered within the renal tissue. Moreover, the study concluded that the thyroid follicle was dynamic structure, and their sizes depending on the amount of colloid present within .


Article
STUDY THE EFFECT OF FLAX LIGNAN EXTRACT OF LINUM USITATISSIMUM AND CONJUGATED ESTROGEN ON PHYSIOLOGICAL PARAMETERS IN FEMALE RATS

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Abstract

This study was carried out in the animal house of the College of Veterinary Medicine /Basrah University to evaluate the effect of lignan extract of flax seed on haematological, biochemical parameters and histological examination in female rats, compared with conjugated estrogen drug. For this purpose, eighteen female rats weighed (180-200g) and aged (4-5 months) was divided into 3 equal groups (6 rats / group) for 14 days. First group: Drenched 1ml of normal saline (0.9% of NaCl)for 14 days. Second group: Drenched 0.10mg/kg B.W of conjugated estrogen dissolved in normal saline (0.9% of NaCl) for 14 days. Third group: Drenched 20mg/kg B.W of lignan extract of flaxseeds dissolved in normal saline (0.9% of NaCl)for 14 days. Blood samples were collected from heart by cardiac puncture from all experimental animals. These samples are used for the measurement of haematological and biochemical parameter as well as hormonal assy. Thyroid glands, liver, kidneys, ovaries and uterus were removed for histopathological study. The results revealed that, the female rats treated with lignan extract of flax seeds showed an increase in body weight. The result also showed that treated female rats with phytoestrogen had no significant effect on blood parameters except a significant effect on lymphocytes. (P≤ 0.05) in serum lipid profile except HDL compared to control. Moreover the results of biochemical parameters were indicated the affected in female rats treated with lignan extract that the revealed a significant(P≤ 0.05) decrease in serum lipid profile except HDL-Ch of female rats treated with lignan extract compared with the control group. Also the treatment with lignan extract caused significant (P≤ 0.05) increase in LH hormone, estradiol and progesterone compared with .While it has no significant effect on FSH. The treatment showed some ameliorative effect on histological structure of studied organs.


Article
HORMONAL PROFILE OF IRAQI BITCHES DURING VARIOUS PHYSIOLOGICAL STAGES.

Authors: Nazih Wayes Zaid
Pages: 50-66
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Abstract

The study was conducted to investigate the success of inducted estrus and to track the hormonal levels during the estrus cycle, pregnancy and post-parturition in dogs. Ten females, aged 2-3 years and weighted 18-22 kg, were kept in cages at the College of Veterinary Medicine-University of Baghdad. Dogs were mated and tracked till they become conceive. Then, after one month of parturition, they treated with Bromocriptine (Parlodel) a dose of (0.05mg/kg/dog) twice daily to induce estrus. Eight dogs showed estrus and mated again with the same mature dogs. Blood serum was taken once a week from cephalic vein. Follicular Stimulating Hormone (FSH), Luteinizing hormone (LH), Estrogen and Prolactin were measured by using canine Gamma counter kits. In both, normal and induced estrus periods, FSH and LH hormone levels started to elevate in proestrus and estrus phases then they declined in pregnancy months and post-parturition. Estrogen and prolactin hormones had significant differences during the second month in the other non-responsive dogs. Estrogen levels indicated that the proestrus, estrus and treatment period showed higher significant differences (P<0.01) than anestrus period.

Keywords

Dogs --- Post-partum --- Bromocriptine


Article
THE EFFECT OF GALLIC ACID AS PROTECTIVE AGENT ON SOME BIOCHEMICAL PARAMETERS INDUCED HYPERCHOLESTEROLEMIA IN LABORATORY MALE RATS

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Abstract

The role of gallic acid was studied to alleviating some biochemical parameters alterations in hyper cholesterolemia in rats. For this purpose 24 sexually adult male rats were divided randomly in to 4 groups (6 rats each). The experiment was conducted at the animal house of the Veterinary Medicine College–University of Basrah, the control group, rats were injected intraperitoneally ( I.P.) with 0.9 % normal saline (N.S)and were supply with the stander ration. The first treated group were injected intraperitoneally ( I.P.) with 100 mg/ kg gallic acid dissolve in 0.5 ml distilled water daily and were supply with the stander ration. The second treated group were supplied with the stander ration in addition to 1.5% cholesterol of the stander ration . The third treated group were supplied with standard ration in addition to 1.5% cholesterol, and injected intraperitoneally ( I.P.) after one hour with 100 mg/kg gallic acid dissolved in 0.5 ml distilled water The obtained results indicated that the administration gallic acid as protective agent on high cholesterol diet tend to alleviated and improved lipid profile by a significant reduction in the serum total cholesterol (TC),LDL-C and VLDL compared with animals fed cholesterol 1,5% diet, hepatic enzymes activates of AST &ALT in the protective group almost were at the range of normal values and the protective urea blood value reach almost as the normal level as control group.


Article
PREVALENCE OF BRUCELLOSIS IN BUFFALOES OF BASRA GOVERNORATE, BASRA - IRAQ

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Abstract

The present study was conducted to identify the prevalence of brucellosis among buffaloes in Basra governorate, via examination of serum samples from 250 she buffaloes reared in different Basra reigns. Sera were examined firstly by rose Bengal test (RBT) followed by indirect enzyme linked immunsorbent assay (Elisa). The result of RBT indicated that from 250 buffaloes serum samples there were 27(10.8%) positive against Brucella abortus antigen. Elisa test was performed on 88 sera samples that included a 27 RBT positive sera and other 61 negative sera, and the result revealed that 21( 23.8%) seropositive sera for Brucella abortus. According to the regions of Basra Governorate the percentage rate of brucellosis were indicated in: Al Hartha 6(5,28%) then Al Qurna 5(4,4%), Al Dear 4(3.5), Al Zubair 3(2.6%), Al Medaina 2(1.76%) and Al Tanooma 1(0.88%). More over, infection in older animals found more significant ( P < 0.05) than in youngness, beside that infection rate were high in pregnant buffaloes in compared with non pregnant animals. Conclusion: the brucellosis of buffaloes in Basra governorate were caused by B. abortus and were more prominent in pregnant animals, therefore animals screening of suspected animals was advised,

Keywords

brucellosis --- buffaloes --- Elisa --- Basra


Article
PHYSIOLOGICAL AND BIOCHEMICAL CHANGES FOR 5- FLUOROURACIL DRUG (ANTI-CANCER) IN CANCER PATIENTS

Authors: Baida R. Ali
Pages: 87-99
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Abstract

The study was conducted to determine the effect of the 5-Fluorouracil (5-FU) drug is a medication used to treat cancer or the knowledge of 5-FU drug(anticancer) effect might help to attribute these side on physiological and biochemical parameters in human by injection into a vein it is used for colon cancer, esophageal cancer, stomach cancer, pancreatic cancer, breast cancer and cervical cancer. 80 blood samples were obtained adult human divided into two groups for each group (n = 40), the first group (control) and the second group treatment with drug 5-fluorouracil. There are a significant decrease (P <0.05) in the blood parameters included parameters Red blood cells count, the total number of white blood cells, platelets , hemoglobin and packed cell volume in men treatment with 5-fluorouracildrugcompared with control group. Causing the 5-FU drug significant decrease(P<0.05) in Triglycerides, cholesterol levels and total protein in the treatment group compared with control group. Also, the result showed a significant increase in Creatinine level of second group in compared with control group, while the results showed non- significant in urea level in human treatment when compared with control group. The results showed a significant decrease (P <0.05) in the level of liver enzymes, (ALT AST and ALP) in the treatment group with 5-FU when compared with the control group.

Keywords

5-fluorouracil --- parameters --- Cancer.


Article
PATHOPHYSIOLOGICAL EVALUATION OF THE PROTECTIVE ROLE OF ALPHA LIPOIC ACID ON DIABETES MELLITUS NEPHROPATHY IN MALE RABBITS

Authors: Jihad Abdulameer Ahmed
Pages: 100-119
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Abstract

In order to estimate the ameliorating effects of alpha lipoic acid (ALA) on the nephropathy of diabetes mellitus (DM) associated with oxidative stress, this study performed in 18 adults male rabbits were divided into 3 equal groups, the group-I was regard as control while group-II Induced DM only by intravenously single dose (150 mg/kg b.w.) of alloxan-monohydrate; group-III was induced DM by intravenously single dose of (150mg/kg b.w.) of alloxan-monohydrate and then treated by (10mg/kg b.w.) daily of ALA intraperitoneally for 4 weeks. The biochemical results showed significant (P≤0.05) decreased of the serum values of GSH, CAT and SOD as well, significantly (P≤0.05) increased of MDA, peroxynitrate, creatinine and BUN of group-II when compared to control; also the result of group-III showed nonsignificant (P˃0.05) differences of GSH, SOD, MDA, creatinine, BUN and peroxynitrate when compared to control. The histopathological and histochemical results of kidney of group-II showed moderate thickness of basement membrane of glomeruli with compressed capillaries as well infiltration of glyco-proteinaceous materials in glomeruli and around renal tubules with some vacuolation of these tubules and mesangial cells; while the results of group-III showed normal architectures with very mild degree of vacuolation of few renal tubules in addition to disappearance of glyco- proteinaceous materials in this group; in conclusion, ALA had a dual protective effects on nephropathy by scavenging the oxidative stress free radicals and enhanced insulin metabolism.


Article
ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION

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Abstract

Eighty three local isolates of fungi were isolated from different resources (Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream, Iranian cream, Roquefort cheese and soil). These isolates were purified and identified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8 Aspergillus terreus,3Aspergillus parasaticus,3Alternaria spp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and 7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxic isolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) were removed.Aspergillus niger which was isolated from maize was choosing as the best lipoxygenase producer after Primary and secondary screening. The growth of the selected isolate colonies had the largest proportion than the Colonies of Penicillium sp. and Trichoderma sp..all so the same isolate had high enzymatic activity 801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120) units/ml respectively.


Article
AN IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOCRINAL CELLS IN THE EPITHELIUM OF THE DUODENUM MUCOSA OF TURKEY ( Meleagaris gallpava )

Authors: Abdulrazzaq Baqer Kadhim
Pages: 136-145
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Abstract

in some parts of the intestinal tract, which play an important and primary role in the secretion of certain hormones that regulate important organs of the body. The aim of this study is to examine the site endocrine cells in the mucous layer of the five male Turkey. The areas where endocrine cells in the mucous layer of the five male in the Turkish examined by using immunohistochemistry technique material. Samples were taken from five birds from the Turkish males ages ranging from 1-2 years. It was used immunohistochemistry textile technology and (ChromograinnA), which is a special detector Endocrine intestinal cells, four types of hormones. Immune chemical examination in the mucous layer duodenum and the presence of hormones, glucose insulin tropic polypeptide(GIP) results showed (GIP) and cholecystokinin (CCK) glucagon-like peptide2 (GLP-2) in epithelial cells group along the internal axis of the villi, chemical immunological results for many of the sections shown note chromogranin A detector particularly for intestinal endocrine cells, the cells that cholecystokinin(CCK) , glucose insulin tropic polypeptide contain hormones (GIP) and glucagon peptide GLP-1 2)) containing this reagent indicating confirm the location of these cells, a gastric endocrine cells . The presence of these hormones in the mucous layer of the Duodenal decreases as we move away from the duodenal


Article
HISTOLOGICAL STUDY OF THYROID GLAND IN CASE OF EXPERIMENTALLY INDUCED HYPOTHYROIDISM BY CARBIMAZOLE IN DOMESTIC FEMALE RABBITS (Lepus cuniculus domastica)

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Abstract

The present study aimed to investigate the histological ,histochemical changes of thyroid gland in case of induced hypothyroidism by carbimazole in domestic female rabbits . Histological results revealed many histpathological changes compared with the control group there are hyperatrophy of follicular epithelium , hemorrhage ,edema and damage in the epithelium lining of the follicles. Histochemical results revealed alterations in normal distribution of glycoprotein inside follicles of thyroid gland in hypothyrodic groups.

Keywords

glycoprotein --- Edema --- hypothyroidism

Table of content: volume:16 issue:1