Table of content

Al-Qadisiyah Journal of Veterinary Medicine Sciences

مجلة القادسية لعلوم الطب البيطري

ISSN: 18185746 23134429
Publisher: Al-Qadisiyah University
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

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Scientific Journal of Veterinary Medicine Science, Issued by College of Veterinary Medicine, University of Al-Qadisiyah

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جمهورية العراق - الديوانية- جامعة القادسية- كلية الطب البيطري
Iraq-Al-Diwaniyah, University of Al-Qadisiyah, College of Veterinary Medicine.
Email of Journal: vmjou@qu.edu.iq
Email of secretary:Saad.Ghmeiss@qu.edu.iq
Mobile: +964 7809236348
Web site: http://qu.edu.iq/vmjou

Table of content: 2017 volume:16 issue:1

Article
Comparative study of the conformational parameters head and neck traits in Holstein and local breeds of dairy cattle

Authors: Muslim F. Diwan
Pages: 1-4
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The objective of this study was to compare the morphological traits of the head and neck in Holstein and local breeds cows which carried out in AL- Diwaniyah city, to assess these traits of the head and neck in both Holstein and local breed cows; 140 animals were selected from each breed aged about (48 ± 4), to investigate the difference in measurements between them in the following parameters; the head length (HL), the line between medial canthi of the eyes (BE), the head side length (SL), head-neck collar (HNC), neck-body collar (NBC), neck length (NL) and mandibular width (MW). The results revealed showed that the Holstein breed cows had the superiority and increment significantly (p≤0.05) in all traits which had been measured (HL, BE, SL, HNC, NBC and MW) in Holstein cows when compared with local breeds. As followed 58.94±0.15; 50.27±0.11, 22.87±0.08; 19.6±0.09, 62.92±0.08 ; 46.5±0.08, 92.3±0.13 ; 59.58±0.07, 130.78±0.12 ; 120.35±0.12 and 23.23± 0.08; 22.76±0.08 (cm) respectively. Except the (NL) parameter which had the significantly (p≤0.05) and superiority in the local breed cows when compared with Holstein breed 44.0.7±0.09 and 42.64±0.11 (cm) respectively.

Keywords

head --- neck --- conformation --- Holstein --- local.


Article
Serological investigation of Brucella spp. and Toxoplasma gondii in horses by using rose Bengal and toxoplasma latex tests in Dhi-Qar province–Iraq

Authors: Jalil Abed Gatie --- Saleem Amin Hasso
Pages: 5-8
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To detect the infection by Brucella spp. and Toxoplasma gondii. This study was conducted using a screening Rose Bengal and Toxoplasma latex tests on 100 blood samples collected from horses (18 male +82 female) average of age (1month-25 years) living in different areas in the districts of Dhi-Qar province in southern Iraq, where results showed a spread of Brucella spp. antibodies germs by 8% and the spread of the parasite Toxoplasma gondii antibodies 21%. This study showed the spread of infection by bacteria Brucella and Toxoplasma parasite in horses in Dhi-Qar. province-southern Iraq.


Article
Serological diagnosis for Hypoderma infestation in cattle of Wasit province by using of an indirect-ELISA

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The aim of present study was to detect the specific Hypoderma IgG-antibodies in blood samples of cattle by using of indirect-ELISA. For this purpose, an overall of 476 cattle from different regions in Wasit province / Iraq, were submitted for blood samples collection and then for serological assay that revealed on 249 (52.31%) as a total result for serologically positive cattle. Also, the study was discussed the seropositive prevalence of hypodermosis with a number of epidemiological risk factors (age, sex, breed, and herd management), which showed that the highest seropositive rates have been reported in 3 years group (59.39%) more than 3 years group (43.72%) for age factor, in females group (56.72%) more than males group (36.54%) for sex factor, and in bad group (59.48%) more than good group (32.81%) for herd management factor. Statistically, at a level of (P0.05) the significant differences were reported within the age, sex, and herd management factors; but it was not detected between both groups of breed factor (local and cross-breed).


Article
The effect of garlic and neomycin supplementation in diet on productive and some blood parameters of experimentally infected broiler chickens with Salmonella typhimurium

Authors: Afrah Sabeeh Mhyson
Pages: 15-22
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The present study was carried out in order to demonstrate the effect of dietary supplementation of garlic and neomycin in reducing the experimental infection of broilers with Salmonella typhimurium &the improvement of productive, physiological parameters all over the duration 42 days.200 broiler chicks(Hubbard flex) were divided into four groups: T1 (negative control):chicks fed on diet without any addition without Salmonella challenge,T2:chicks fed on diet mixed with 5% garlic powder (at 3 day age) plus Salmonella challenge,T3:chicks fed on diet with neomycin 0.5% (at 3 day age) plus Salmonella challenge,T4 (positive control):chicks fed on diet free of any addition ,but challenged with Salmonella. The results showed that the addition of garlic powder to the diet of broilers caused significant reducing S.typhimurium infection resembling to that of neomycin addition both produced significant decreasing(P≤0.05) in the number of excreted Salmonella in the feces and the mortality rate in the treatments T2 and T3 in comparison with T4.The productive parameters showed significant increasing(P≤0.05)in body weight and weight gain inT1,T2,T3 when compared withT4 in spite of no significant differences in total food consumption and feed conversion ratio among the four treatment, on the other hand the blood parameters showed significant increasing(P≤0.05) in RBC count and PCV in T1,T2,T3 in comparison with T4, while the percentage of hemoglobin showed non-significant increasing in T1,T2,T3when compared with T4,and there are significant increasing in WBC count in T2,T3,T4 when compared with T1, also there are significant decreasing in the three treatments when compared with T4 in L/H ratio, while there was no significant difference in differential WBC between all the treatments, As conclusion, the results of our study had demonstrated the antibacterial activity of garlic and neomycin against the experimental infection with Salmonella via enhancement of resistance and immunity besides the improvement of the productive and blood parameters.


Article
Detection of cholecystokinin and glucagon like peptide in small intestine of Awassi sheep

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The enter endocrine cells in small intestine of sheep secreting some hormones that play key roles in regulation of certain important organs. The endocrine cells of GIT are generally divided into two types, the open and close type.The aim of this study was unveil the relative frequency and regional distribution of enteroendocrine cells in some portions of small intestine of the Awassi sheep, detecting by using immunohistochemistry techniques. Specimens of small intestine from ten of both sexes with different ages of sheep Ovis aries were used. The Immunohistochemistry technique formed using two types of hormones cholecystokinin (CCK-8) and glucagon like peptide (GLP-1). Result of immune detection findings demonstrated that in part of small intestine (duodenum, jejunum and ileum) there is clearly expression of the CCK-8 and GLP-1 subset of cells along the ‎villus and crypts. The cells are contained gut hormones appeared to be either triangular or flask-like in shape. I-cell which contain CCK-8 increase proximally of small intestine and decrease caudally, while L-cell which contain GLP-1 decrease proximally but increase caudally of small intestine


Article
Effects of different doses of aspirin on the liver and kidney functions of the female albino rats

Authors: Nuha SH. Ali
Pages: 31-37
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Abstract

The confer study was carried out to examines in detail the effect of Aspirin on the liver and kidney function of the female albino rats. (15) mature female albino rats (150-170 gm)were divided in to three groups :1st group which treated as negative control group ,it was drenched only 0.2 ml of PBS.2nd group was drenched with Aspirin (10mg/kg of B.W once daily for 30 days).3rd group was drenched with Aspirin (20mg/kg of B.W once daily for 30 days).Our results showed that 2nd and 3rd groups demonstrated significantly increased (P≤0.05) in the values of plasma GOT and GPT enzymes compared with control group. In the values of plasma total protein, the 2nd and 3rd groups showed meaningfully decreased (P≤0.05) in the relation with the control group. The values of plasma creatinine and uric acid, the 2nd and 3rd groups showed significantly increased (P≤0.05) as compared with control group. On the other hand, the microscopic examination of the histopathological sections of livers of all the treated and control groups showed that the 2nd group demonstrated congestion of central vein and presence of radially arrangement of hepatocyte around the central vein ,mild dilation of sinusoids ,mild hemorrhage and mild infiltration of inflammatory cells. In 3rd group, the histopathological examination of livers showed infiltration of inflammatory cells mainly macrophage and lymphocytes, congestion and hemorrhage in the hepatic tissue and vaculation of hepatocyte. In kidneys of 2nd group showed high cellularity of the glomeruli, mild infiltration of inflammatory cells, mild hemorrhage in the renal tissue and few dilation of renal convoluted tubule. The kidneys section of 3rd group showed atrophy and small in size of the glomeruli with marked dilation in the renal convoluted tubules. In addition, there is degeneration in the epithelial cells which lining the renal tubules and hemorrhage along the renal tissue.

Keywords

Aspirin --- liver --- kidney


Article
The expression of gonadotropin releasing hormone receptor gene in ovaries and uterus cells of Iraqi and Damascus goat breed

Authors: Alaa kamil Abdulla
Pages: 38-44
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Iraqi goats have a major economic role in production of meat, milk and leather as well as it considered a financial source for owners as reproduce twice a year, yet the Damascus goats have great importance than Iraqi goats owing to the number of twin births. The gonadotropin releasing hormone (GnRH) and its receptors have great importance in the reproduction and eugenics. To make a comparison between the Iraqi and Damascus goats in terms of this receptor gene expression in the ovaries and uterus tissue cells, the study was performed, in which used the (∆Ct Using a Reference Gene method) by quntitive -real time PCR technique. Results were found a significant difference (p<0.05), as the gene expression of (GnRH-R) higher in the ovaries and uterus tissue cells in Damascus goats compared with the Iraqi goats. In conclusion; the multiple pregnancies of twins in Damascus goats may be due to an increase gene expression of (GnRH-R) in the ovaries and uterus tissue


Article
Effect of iron dextran injections on some synovia markers of Iraqi sport horses

Authors: Qayes Taref Ali
Pages: 45-49
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In the past decades, many veterinarians and trainers had been tried to use iron preparations as performance enhancers for racing, or for treatments such as ˝anemiȁ. The study was conducted on (12) Iraqi sport horses. The principal aims of the study were to investigate the effect of iron dextran on some synovial fluid constituents at the rest and the endurance exercise of Iraqi sports horses. The physical markers of synovial fluids of the sports horses which are included in this study, shows: (TG-1) (gallop and iron dextran treatment) are mostly slight turbid, dark yellow, very low viscid, and normal mucin clot formation. (TG-2) (Iron dextran only) are mostly clear, yellow, viscid and normal mucin clot formation. While (CG-3) (distil water only) are mostly clear, pale yellow, viscid and fair mucin clot formation. The effect of iron dextran on some biochemical markers of the synovial fluid constituents of the sports horses which included in the study were shows as in table-3: the PH value of (TG-1) was very low than normal 6.15±0.85 which was significantly different at P≤0.05 with the PH value of the (TG-2) 6.64±0.36 while the PH of (CG-3) was within normal value 7.57±0.85 and there was no significant different at P≤0.05 with (TG-2). The WBC value of (TG-1) was 8.61±0.38 (WBC/mm3) which was different significantly at P≤0.05 with (TG-2) 6.95±0.60 (WBC/mm3) but (TG-2) was not different significantly at P≤0.05 with (CG-3) 5.45±0.20. (WBC/mm3).The glucose value of (TG-1) was reduced than normal 52.89±1.58 (mg/dl), which was different significantly, at (P≤0.05) with (TG-2) 51.48±1.12 (mg/dl), but (TG-2) was not different significantly at (P≤0.05) with (CG-3) 53.14±1.04 (mg/dl). The total protein value of (TG-1) was increased than normal 3.68±0.38 (g/dl) which was different significantly at P≤0.05 with (TG-2) 2.45±0.23(g/dl) but (TG-2) was not different significantly at (P≤0.05) with (CG-3) 1.52±0.21. (g/dl). The hyaluronic acid values were significantly different at P≤0.05 between all groups, (TG-1) 0.33±0.03 (mg/ml), (TG-2) 0.21±0.03 (mg/ml), and (CG-3) 0.37±0.03 (mg/ml) respectively. The alkaline phosphatase values also were significantly different at P≤0.05 between all groups, (TG-1) 38.44±1.61 (IU/L), (TG-2) 27.25±0.22 (IU/L), and (CG-3) 25.31±1.79 (IU/L), respectively. In conclusion, iron injections had no any improvement to the sport horses' synovia.

Keywords

Iron --- synovial fluid --- Iraqi --- sport horses


Article
Comparative study on early pregnancy diagnosis by three different techniques in cows

Authors: Baraa D. Al-Watar
Pages: 50-54
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The aim of this study was to determine the best method, most appropriate and high precision method between rectal palpation, ultrasonography and measuring level of progesterone for diagnosis of early pregnancy in cows. 20 cows were examined in Teaching Hospital / College of Veterinary Medicine / Mosul University, at the period between 1/11/2013 - 1/3/2014, cows undergone early pregnancy diagnosis using three different methods at 45, 60 and 90 days of pregnancy. Results of rectal palpation showed that the percentage of pregnancy was 0% 60% 85% at days 45, 60 and 90 of pregnancy, respectively, while results of ultrasonography showed that diagnosis early pregnancy was 30, 80 and 100 at days 45, 60 and 90 of pregnancy, respectively. Results of estimation of progesterone concentration by ELISA technique showed that pregnancy diagnosis was 60% 85% 100% at days 45, 60 and 90 of pregnancy, respectively. In conclusion, the best and fastest way to diagnose early pregnancy in cows was measure concentration of progesterone in blood serum of cows then followed by ultrasonography, while method of rectal palpation was inefficient in detecting early pregnancy in cows.


Article
A prevalence Study of ectoparasites on the long-eared hedgehog (Hemiechinus auritus) in AL-Muthanna province-Iraq

Authors: Farhan A. Eabaid --- Muhammed Oda Mallah
Pages: 55-59
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This study was carried out in AL-Muthanna province during the period from November 2014 to May 2015.A (41) animals of long eared hedgehog Hemiechinus auritus(27 male, 14female) were collected from different area of Al-Muthanna Province (Rural and Urban regions). A total number of 41 hedgehogs, 19 were infested with ticks (46.34%). All ticks were belonged to Rhipicephalus turanicus species, also the fleas of the species Archaeopsylla. erinacei were found on 12 hedgehog of 41 (29.26%) examined hedgehog. Also the results showed, that the rates of infestation with R. turanicus and A. erinacei were difference significant (P<0.05) between the sexes (male and female) of hedgehogs that the rate of infestation with ticks was 12(44.44%) for male and 7(50%) for female, while the fleas was 8(29.62%) for male and 4(28.57%) for female. Highest occurrence of infestation in both tick and flea was in April 62.5%. Prevalence of ectoparasite in March, April and May was 50% and 59.1%. While there are no infection in November, December, January and February. The significant variations were no observed among months in both ectoparasite species (P>0.05).


Article
Molecular detection of invA gene for Salmonella spp. isolates from poultry in Babil province-Iraq

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A total of 150 poultry samples (age from 1 to 49 day) were collected from different locations at Babylon province ) Al-hashimiya , Al-madhatiya and Al-Qasim ) from November 2015 to April 2016. These samples were collected from different part of the body (Liver tissue, Yolk sac content, and cecal swab). Salmonella spp. was isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E and serotyping by monovalent antisera. Polymerase chain reaction (PCR) was used to detect invA of Salmonella spp. The results revealed that the rate of Salmonella isolates from poultry specimens were (11) 7.3% using cultural and biochemical tests, the results of serotyping revealed these isolates belong to Salmonella spp. The PCR technique was used to detect invA gene, these Salmonella isolates appeared to contain this gene since DNA amplification showed one distinct band (size 389 bp) when electrophoresed on agarose gel. The results of this study revealed that the PCR technique had a high specific in detection of Salmonella spp. When compared with other conventional detection methods

Keywords

Poultry --- Salmonella --- PCR --- invA gene


Article
Molecular determination of extended spectrum b-lactamases antibiotics resistance genes in E.coli isolated from diarrhea in cattle

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None response to the treatment by an antibiotic called antibiotics resistance result from some genes called resistance genes .This mechanism is widespread in most of the bacteria, like E.coli . All of the extended resistance genes called (ESBIS) is a typical example for study of some genes that resistance beta-lactam antibiotic is subject of this research. Fifty feces sample were collected from cattle suffering from diarrhea in alqaissiyah city were cultured on selective media for E.coli , then DNA was extracted from all E.coli isolates for antibiotic resistance gene detection by PCR ; The results of this study revealed the prevalence of B-lactamase gene four B-lactamases genes in E.coli blaAmpc gene were (91.4%), the blactx-m gene were (80%), blaTem were (62.8%) and finally and blaSHV gene were (22%) among isolates E.coli ; blaAMPC gene has high prevalence than others genes while blaSHV was a lower percentage than other genes .


Article
Menopon gaillinae lice in the golden eagle (Aquila chrysaetos) and Marsh harear (Circus aeruginosus) in Najaf province-Iraq.

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Our study considered as the first work on ectoparasites of the Golden eagle (Aquila chrysaetos) and Marsh harear (Circus aeruginosus) in Iraq. Overall, we examined 17 eagles for the period from 01Nov2016 till 25Feb2017, out of which 4were found infected (23.5%). All infected birds were female. Aquila was hunted from Najaf sea area. Under the wing and between feathers of Aquila grossly examined for detect any parasites. Lice of genus Menopon gaillinae isolated from 4 eagles, from under the wing area. Infected eagles suffering from skin redness. 38 parasites isolated from infected eagle, we prepared a slide from these louse for spp. classification. This study on the first hand record of shaft louse (M. gallinae) in Golden eagle and Marsh harear in Iraq

Keywords

: eagle --- lice --- Iraq --- Najaf --- Menopon gaillinae.


Article
Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk

Authors: Hayder N. Ayyez
Pages: 75-78
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Over the last years, the number Coxiella burnetii infections increased throughout the wourld. A molecular detection and phylogenetic analysis were performed in Al-Diwaniayh city in which the first phylogenetic documentation of C.burnetii reported in this area with accession numbers KY576802, KY576803, KY576804 and KY576805. Molecular detection and phylogenetic analysis of C.burnetii targeting the transposase gene partial sequence was done on the milk samples from 50 apperently healthy goats. DNA sequence was amplified in 6 out of 50 (12%) milk samples collected from different regions of Al Diwaniya city. Four of these amplicons were submitted to sequences analysis and gave 99% nucleotides sequence similarity. Also the amplicon sequences of local strain were compared with global C.burnetti sequences in NCBI which revealed close related to NCBI-Blast C.burnetii transposase gene of Indian (AB84899.1) and Brazilian (JF970261.1) strains, whereas there was genetic variation to NCBI-Blast C.burnetii transposase gene for USA (DQ379976.1), Portugal (EU009657.1) and Taiwan (EU000273.1). This work confirm goats infection and shedding the C. burnetii in Al-Diwaniayh, Iraq and determined the phylogenetic tree of local strain of this bacterium.


Article
Cytological and bacterial analysis of transtracheal aspirate from stray cats in AL-Qadisiyah province/ Iraq

Authors: Muthanna Hadi Hussain
Pages: 79-81
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This study was conducted to count the cytological parameters; total protein (TP), white blood cells count WBCs and differential (WBCs); from transtracheal aspirate (TTA) and microbial detection from thirty cats in Al-Qadisiyah province. Both genders were involved and the cats ranged in 1-3 years old. The mean total protein measured by the spectrophotometer was (89±0.04) mg/dl, mean WBCs was (925±0.7) cell/µl and the mean differential WBCs record 61% alveolar macrophages, 31% eosinophils and 8% neutrophils. Microbial investigation has revealed that respiratory tract of cats have no flora and we could not observe any respiratory infection in the captured cats; it may be related to the physiological and immunological adaptation of the respiratory system through the presence of the alveolar macrophage with a high percentage concerning other WBCs. There was no obvious regard to gender or age on these limitations.

Keywords

cytology --- transtracheal --- total protein --- cat


Article
Macroanatomical and histological study of kidney in porcupine (Hystrix cristate)

Authors: Abdularazzaq baqer kadhim
Pages: 82-86
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Involve this research grossly and histological study of the kidney of porcupine hystrix cristate. The study was achieved on ten kidneys from five adult (male and female porcupine (hystrix cristate). They were cautiously dissected and introspect after used the chloroform for anesthesia directly. The macroanatomical studies established the two kidneys of porcupine. the kidneys are located in retroperitoneal region in the posterior part of the abdomen on each side of the lumber vertebra .The renal papilla are consider end the outer cortex and inner medulla together as show after anatomical distinct regions of kidney. The indicate width of cortex and medulla of right kidney (5.500±0.371) mm, (5.588±0.180) mm respectively while the indicate width of cortex and medulla of left kidney (5.780±1.792) mm, (5.492±0.205) There is no obvious border between the cortex and medulla. The renal corpuscle is sphere-shaped created of glomerulus, which is consiste of Bowman’s capsule. Proximal complicated thin tubules carctrestic by have widest lumen and internally inside layer by epithelial cells are cuboidal which latent on the basal membrane. This cells described by have ground eosinophile cytoplasm, big center nuclei and the apical surface covered microvilli. as the epithelial lining of low volute tubules have widespread lumen and cuboidal cells have ground cytoplasm was visibly fewer eosinophilic campier that of the upper tubules and the apical surface of the distal complicated tubule was discovered by short microvilli


Article
Genotyping of Brucella melitensis isolated from human and sheep in Iraq

Authors: Khetam Qaid M. AL-Hamdawee
Pages: 87-92
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Brucellosis is a widespread endemic zoonotic disease as well as significant impact on human health together with ruminant’s manifests as abortions or other reproductive problems in different animal’s species. A specific sensitive PCR and DNA sequencing technique employed in this study to provide the first Iraqi profile about B. melitensis in Genebank to overcome the determinates posed by the others accurate diagnostic methods like isolation and serotyping. In Women's Maternity and Children Hospital, and Gynecology Outdoor Patient (OPD) in the city One hundred twenty two (122) samples (107 serum and 15 aborted fetus) collected from a women have a history of abortion and either aborted fetus, serum tested directly with rose Bengal while aborted fetus submitted to culturing. Seventy four (74) blood samples collected from different ewes with abortion history tested with Rose Bengal test conducted the positive cases to PCR then DNA sequencing. Out of 196 samples 6 samples (2 human and 4 sheep) were positive for PCR technique, while only 3 partial gene sequenced samples were identified as B. melitensis revealed three different biovars available under accession number (KX793714.1, KX793715.1, and KX793716.1) in Genebank A1, A2 strains isolated from sheep and A3 human strain. B. melitensis was the only species detected, ensuring its highest zoonotic potential in Brucella genus. A1 and A2 Sheep isolate were shown closed related to NCBI-Blast Brucella melitensis biovar 3 (DQ086122.1). Whereas, the Brucella spp. A3 Human isolate was shown closed relation to NCBI-Blast Brucella melitensis biovar 1 (DQ086119.1) and ( DQ086121.1).


Article
Comparative study of some hormones during rutting season in dromedary camel

Authors: Najlaa S. Ibrahim --- Nazih W. Zaid
Pages: 93-98
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This research was aimed to study the effect of age on steroids, cortisol and thyroids hormones during rutting seasons in camels. Blood serum were taken from 103 healthy camels from Al-Najaf province slaughterhouse, samples were divided according to age into two categories: pre-pubertal animals 1-4 years and mature 5-8 years. The estrogen, progesterone, cortisol, T3 and T4 hormones were tests by using Gamma counter. The recent results indicated that the age had an effect on estrogen, progesterone, cortisol, T3 and T4 and there were a significant differences (P<0.01) between mature and pre-pubertal ages. There were also increases in those hormones during rutting season in both age groups. We conclude that the age and rutting season had an effect on steroids, cortisol and thyroid hormones levels in dromedary male camels

Keywords

dromedary camel --- rutting --- hormones


Article
Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah Province- Iraq

Authors: Khalefa Ali Mansour
Pages: 99-104
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In this study, fifty samples from nodules of skin tissue collected from cattle were clinically supposed to be infected with lumpy skin disease (LSD) from deferent area in Al-Qadisiyah province .These specimens were provided to PCR assay. The endpoint of conventional PCR assay was sent to sequence analysis. The results revealed that thirty-nine samples out of fifty were positive to lumpy skin disease virus at 752bp PCR product of p32 gene, the sequence analysis of five positive samples were done as a confirmative diagnosis to PCR assay. The sequences of these five samples can be found under the accession numbers (KR066462.1,KR066463.1,KR066464.1,KR066465.1 and KR066466.1) at NCBI-Gen Bank submission. In conclusion; the sequence analysis of five local isolate of lumpy skin disease virus was close related to NCBI-Blast reference lumpy skin disease virus isolate (KU720359.1) and Kurdistan isolates, whereas others "Turkish and Egyptian isolates" were different.

Keywords

LSDV --- P32 gene --- PCR --- sequence analysis --- Phylogeny


Article
First molecular diagnosis of ovine pulmonary adenocarcinoma in Awassi sheep in Iraq

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Background: Ovine pulmonary adenocarcinoma (OPA), formerly known as sheep pulmonary adenomatosis is a contagious tumor of the respiratory epithelium. Aim: The aim of this study to investigate about ovine pulmonary adenocarcinoma (OPA) in Iraqi Awassi sheep. Material and methods: One hundred and twenty blood samples from Awassi sheep clinically had respiratory problematic were collected as well as sixty tissues samples. included twenty of each of (lung, lung fluid, mediastinal lymph nodes ) were collected from lungs with abnormal pathological lesions , all samples tested by RT-PCR by using specific primers and probe were designed in this study by using conserved region in envelope protein gene (NCBI-Gen Bank Code: KT279065.1) . Results: Ten sheep (5.55%) were found to be positive for JSRV by RT-PCR with higher infection rate in lung fluids 20% , mediastinal lymph nodes 10%, lung tissues 5% , and the less infection rate in blood . 2-5 years aged sheep were more susceptible for infection than other age ,but gender of examined sheep not affected on APO infection . Conclusion: The first detection of Ovine pulmonary adenocarcinoma (OPA) in Iraq in Awassi sheep in field and abattoir.

Keywords

Adenocarcinoma --- Jaagsiekte --- Sheep --- RT-PCR --- Iraq


Article
A study on plasma estradiol and progesterone profile at days 11, 12 and 13 post ovulation undergone embryo transfer at day 7-post ovulation in local Egyptian mares.

Authors: Khalid Mohammed Karam
Pages: 111-115
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This study was conducted on 28 recipient local mares after synchronizing and recovery of embryos of Arabian mares (donor mares) and transferring them to recipients on day 7 post ovulation, all mares were raised in the studs of police academy –Cairo Egypt under same circumstances in the breeding season from February till may of 2013. Seventeen recipient mares were pregnant and 9 mares were non pregnant when ultrasound pregnancy check was done on day 21 post ovulation, blood samples were taken on days 11,12 and 13 to detect the steroidal hormonal profile (estrogen and progesterone) via Elisa technique of the recipient mares plasma steroid level and its role in early pregnancy and maternal recognition. The results were significantly higher (P≤0.05) between pregnant (n=17) and non pregnant (n=9) recipient mares in the plasma progesterone concentrations which were 10.99±0.16 vs 9.59±0.11, 12.69±0.16 vs 11.79±0.22 and 14.4±0.15 vs 13.78±0.23 ng/ml on days 11,12 and 13 post ovulation respectively, significant difference(P≤0.05) was observed when comparison between pregnant mare’s plasma progesterone concentrations on days 11,12 and 13 post ovulation. Plasma estrogen concentration were significantly higher (P≤0.05) in non pregnant and pregnant mares which were15.17±0.18 vs 14.84±0.14, 14.74±0.27 vs13.94±0.12 and 14.14±0.3 vs13.12±0.16 pg/ ml on days 11,12 and respectively, on the other hand when comparison between days11,12 and 13 plasma estrogen levels were significantly different (P≤0.05) in pregnant mares while no significant difference was found in the same days between non pregnant mares, thus might be the main reason for early embryonic death when detected in early pregnancy check via ultrasonography in 21 days post ovulation.


Article
Microscopic, serological and molecular detection of Babesia bigemina in buffaloes (Bubalus bubalis) in Wasit province- Iraq

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The goal of present study was to detect an incidence of Babesia bigemina in apparently healthy buffaloes using of three different diagnostic assays. An overall of 179 buffaloes (Bubalus bubalis) in some rural regions in Wasit province /Iraq, were submitted for collection of blood samples during the period of December 2016 to February 2017. Initially, a thin blood smear slides were prepared and stained with Giemsa stain for microscopy, afterwards, the blood samples have been centrifuged for obtaining of serums and blood clots that examined by an indirect-ELISA and PCR, respectively. The overall results of testing all samples were revealed on 3 (1.68%), 46 (25.7%) and 21 (11.73%) positive animals by microscopy, indirect-ELISA and PCR, respectively. The significant differences (P0.05) were showed between the results of three diagnostic tests, and within the cross-classification values of microscopy to indirect-ELISA, microscopy to PCR, and indirect-ELISA to PCR.


Article
Detection of endoparasites and hematological parameters changes in )Gallinula chloropus)

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Abstract

Gastro-intestinal parasites and blood parameters were examined in thirty five Moorhen birds Gallinula chloropus that hunted in different areas in AL-Diwaniya city during April –August 2016. Endoparasites were examined and diagnosed according to classical standard parasitological methods which revealed 57.14% of moorhen bird were infected endoparasite Protozoal infection with higher infection rate 20% then Cestodal infection 14% and the infection by Nematoda spp and Trematoda spp was 11.42 % respectively. The blood indices related to these parasite intestinal showed significant decrease of RBC count,PCV and Hb concentration, While WBC count increased significantly due to endoparasite infection


Article
Serological and molecular detection of Toxoplasma gondii in Columba livia hunting pigeons of AL- Qadisiyah province

Authors: Hiba Riyadth Jameel Al-abodi
Pages: 128-133
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Abstract

Toxoplasma gondii were detected and diagnosed in 80 Columba livia birds at the province of Al-Qadisiyah during the period started from January 2016 until June 2016 using latex test and rapid cassette test ,Moreover molecular diagnosis with Polymerase Chain Rraction technique for identification of B1 gene had been also used Results showed that antibodies were detected in 11 samples out of 80 (13.75%). of Columba livia pigeons in Al-Qadisiyah province, Since significant highest (P≤0.05) titration were indicated at 1/80 (36.37%),whereas the lowest titer indicated at 1/40 (9.09) and According to type of antibodies in Columba livia toxoplasmosis suspected samples using rapid test cassette, results were indicated that Six samples out of 80 (7.5%) were found positive. However IgG, IgM and IgG plus IgM were found in (33.34%),(16.66) and (50) respectively with no significance difference, furthermore results of PCR technique for detection of B1 gene revealed that 5% of samples from the Columba livia were only found positive ,Moreover the B1 gene were had a molecular weight of the private 399bp.It had been concluded that Columba livia birds were found infected with T.gondii with possible transmission to human being


Article
Using the fungus Entomophthora muscae (chon) Fresenius to eliminate some larval roles of Musca domestica

Authors: Walaa Yas Lahmood
Pages: 134-138
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Abstract

Studied effect serial concentrations from spores filtrate of fungus Entomophthora muscae on some larval roles of musca domestica in laboratory. Results were made clear that the insect roles are sensitive to fungus,and treated the food larva of musca domestica and sprinkle it by concentration 2.8×106 , 2.8×107, 2.8×108 (spore/ml) has led to get rates of destruction of cumulative faculty certified on the concentration and time its magnitude 16.60 , 47.67, 53.30 % respectively , also recorded some phenotypic distortion infected dead larva represent by contraction and blackening body. The treatment of pupael by sprinkling the previous fungus concentration recorded rate of destruction of accumulative faculty its magnitude 13.33, 26.67, 33.33% respectively, also the rates emergence of adults ranged between 66.67 – 86.67 % in comparison with rates of emergence of adults in control treatment 96.67% The results are made clear that adults treatment by sprinkle with last concentration from fungus spore filtrate recorded rates of distraction its magnitude 46.61, 56.67, 70% respectively after one week from treatment

Table of content: volume:16 issue:1