Table of content

Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

Table of content: 2017 volume:16 issue:4

Article
Identification of 27bp Variable Tandem Repeats in Endothelial Nitric Oxide Synthase (eNOS) Gene of Hypertensive Subjects in Kurdish Population from Erbil City

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Abstract

Hypertension is a multifactorial and polygenic disorder where several susceptible genes interact with the environmental factors. Endothelial nitric oxide synthase (eNOS) produces nitric oxide (NO) which is involved in many physiologic regulatory functions. Variable number of tandem repeats in intron 4 of endothelial nitricoxide synthase gene are reported to be associated with blood pressure regulation. In the present study, we examined possible association between the 27 base pair (bp) repeat polymorphism in intron 4 of the eNOS3 gene and hypertension in a Kurdish population from Erbil city. Fifty eight patients with hypertension and 42 apparently healthy controls were included in the study. Genotyping was performed by polymerase chain reaction (PCR). The genotype frequencies for eNOS4b/b, eNOS4a/b and eNOS4a/a were 55%, 23.8% and 21.2% in control group, and 70.7%, 24.2% and 5.1% in hypertensive group, respectively. The eNOS bb genotype (70.7%vs 55%) was found to be significantly associated with hypertension (P=0.000, OR= 7.14: 95% CI; 3.025-16.85) which indicated that the odds of hypertensive is about seven times higher in bb genotype compared to control (OR= 2.38,95% CI;1.55-3.64), whereas eNOS aa were significantly more frequent in controls (21.2 % vs. 5.1 P=0.014) which indicated that this genotype associated with decrease in the risk of having hypertension. (P=0.014)


Article
Biosorption of Chromium ions from Aqueous Solutions by using Date Palm Fibers

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Abstract

This research describes the removal of Cr (VI) ions by adsorption on date palm fibers. This plant is locally available in Iraq and nearby countries. Date palm fibers have porous structure as well as rich in carboxyl, hydroxyl, and carbonyl groups which cause the adsorption process. The biosorption of Cr (VI) ions from aqueous solutions by date palm fibers was studied in a batch experiments as a function of, contact time, metal ion concentration, mass of adsorbent and shaking speed. The adsorption data fit well with Langmuir adsorption isotherm model rather than Freundlich model. The adsorption percent was found to be about 93% when the operating parameters are: mixing speed 250 rpm, particle size 100-200 µm, initial chromium ions concentration of 100 ppm and adsorbent concentration 20 g/L. Two isotherm models were tested; Langmuir and Freundlich. Experimental data was found fitted well by Langmuir equilibrium isotherm model.


Article
The role of Human Cytomegalovirus infection in Iraqi brain tumor patients

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Abstract

A total of (40) astrocytoma patients tissues were enrolled in the present study which include (20) normal surrounding areas from similar tissues used as a control group. The fourty cases represented by formalin fixed paraffin embedded brain tumor tissues blocks and these blocks were collected from the archives of histopathology laboratories at Neurosurgery Teaching Hospital in Baghdad. During the period 2014 to 2015. In our study, the astrocytoma patients were classified into four groups according to their grades (I, II, III, IV). A retrospective study of (40) paraffin embedded samples which were previously diagnosed as brain tumors along with normal unaffected tissues or tissue surrounding the tumors as control were selected from different Histopathology laboratories. All the slides of the paraffin-embedded samples were re-examined and specific sections were selected to be prepared for the techniques (CISH). The Paraffin-embedded samples were sectioned to several sections with (3-4 mm) thickens on charged slides Using Chromogenic insitu hybridization procedure (CISH technique) was used to detect the HCMV on the embedded tissues by light microscope. All grade have positive results for HCMV nucleic acids but the higher percentage (100%) was present in high grades astrocytoma grades (IV).

Keywords

Cytomegalovirus --- Brain tumor --- CISH --- HCMV --- PCR.


Article
Effect of Gamma, Beta, Alpha irradiation and effect of Semiconductor laser on Serratia marcescens (in vitro)

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Abstract

This work evaluated the effect of gamma , beta ,alpha and Semiconductor laser irradiation on Serratia marcescens The experiment included a control and 5 doses of each gamma , beta and alpha irradiation ,which ranged(1.317×10-4 -0.320µSv) and (10.573-96.950µSv) respectively.The total effect of Gamma , Beta and Alpha irradiation on S. marcescens viability was counted after exposure to (137Cs , 90Sr ,60Co , 241Am , 22Na) isotope for 3 hr. respectively, the percentage of killing was highly 83%, 85%, 75%, 90%, 82% respectively . The viable cells was fewer than control (without exposure to irradiation) . This work evaluated also the effect of Semiconductor laser on S. marcescens . experiment included a control and triplicate exposed to Semiconductor laser in power 5 mW , in Wavelength 650 nm . The effect of Semiconductor laser on the viability of S. marcescens was counted and percentage of killing was counted, the number of viable cells of S. marcescenc was fewer than control (without exposure to laser), but percentage of killing was high. Gamma, Beta, alpha irradiation and semiconductor laser was efficient to killing S. marcescens that cause many infection to human.


Article
Phytochemical, Antibacterial and Antioxidant Activities of dodonea viscosa Jacq. extracts Cultivated in Iraq

Authors: Ahmed H. AL-Azawi
Pages: 37-46
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Abstract

In this present study, methanolic and aqueous crude extracts of leaves of Dodonaea viscosa were investigated for their antibacterial activity against Shigella dysenteriae, Salmonella typhi and Bacillus cereus using the agar well diffusion method. The high concentrations of methanolic and aqueous extracts (30 and 40 mg/ml) have inhibitory effects against microorganisms used in this study. The phytochemical analysis of the leaf extracts of D. viscosa were investigated the results show that the methanolic and aqueous extracts contain flavonoids, alkaloids, tannins, saponins, coumarins and steroids. In addition, the antioxidant and free radical scavenging activities were evaluated. The EC50 values of methanolic extract (8 μg/ml) has shown possess DPPH radical scavenging activity compared with reference substances BHT and vitamin C (EC50= 4 and 4.2 μg/ml) respectively.


Article
Genetic Analysis of Coagulation Factor IX (FIX) Gene in Sample of Iraqi Patients With Hemophilia B.

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Abstract

Hemophilia B (HB) is an X-linked recessive bleeding disorder caused by mutations in the coagulation factor IX (FIX) gene. Genotyping patients with HB is essential for genetic counseling and provides useful information for patient management. The aim of this study was to characterize the factor IX gene mutations in 20 unrelated Iraqi males hemophilia B patients from Thi-qar provence. This study was carried out in the Institute of Genetic Engineering and Biotechnology –University of Baghdad, during the period from April, 2016 to April, 2017.Their ages ranged from 4 -30 years .Polymerase chain reaction (PCR) and direct sequencing was performed to detect the mutations in patients. Three functionally regions of the gene (exon 4,exon 5 and exon 7)were screened. Twenty four point mutations were identified, including two missense variant (g15340 del G , p.Aspartic acid convert to Methionine acid) and (.22639 del A, p. Asparagine convert to Threonine) , two mutations were silent (g. 22632 del A, g. 22635 del A), while the rest of mutations were intronic mutations. These results conclude that mutations related with of haemophilia B disease.


Article
Study of some hormonal changes in women with polycystic ovary syndrome in Kirkuk city

Authors: Layla Abd-Al-Sattar Sadiq Laylani
Pages: 55-59
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Abstract

The present study was conducted to find the correlation between certain hormones levels and PCOS. The study used two hundred volunteers (One hundred forty eight with polycystic ovary syndrome and fifty two apparently healthy subjects). Patients were divided to three groups according to their age. The first group was include apparently healthy and patients between 12-21 year, while second group was include age between 22-32 year, the third group was include age 32-41 year. Serological assays showed an increas in LH, Leptin, inhibin b and insulin resistance in all patient groups. Moreover, all parameter levels showed significant increas (P<0.05) in all patients compared with all healthy subject groups. While, FSH levels still normal in both groups and there is no significant changes comparing with healthy groups. It was concluded from this study that the polycystic ovary syndrome led to several hormonal changes.


Article
FAS and FASL genes polymorphisms and their relationship with the incidence of severe oligozoospermia in a sample of Iraqi patients.

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Abstract

The interaction between Fas and FasL plays an important role in triggering the apoptotic pathway. Both Fas and FasL exist as membrane bound and soluble forms. It is suggested that Fas may be a marker of overall apoptosis triggering, at the same time regulating apoptosis by competing with the cell surface receptor. The aim of the present study was to detect the FAS and FASL gene polymorphisms and to demonstrate their relationship with incidence of severe oligozoospermia in a sample of Iraqi patients. Following semen analysis, blood samples were collected from severe oligozoospemic patients (n=50) and normozoospermic subjects (control, n=50). DNA was extracted using DNA extraction Kit (Geneaid Biotech). The genotypes of FAS (-670G/A) and FASLG (-844T/C) were determined by using Taqman (RT-PCR) Kit ( WizPure™ qPCR Master ( PROBE) , south Koria). The results of the present study, as related with Fas -670G to A SNP, indicated that the frequencies of both GA and AA genotypes were significantly (p<0.01) higher in severe oligozoospermia group than in normozoospermia group (30% versus 20% and 60% versus 0%, respectively). Also, as related with FasL -844C to T SNP, the frequencies of both CT and TT genotypes were significantly (p<0.01) higher in severe oligozoospermia group than in normozoospermia group (38% versus 16% and 50% versus 0%, respectively). In conclusion, there was an A allele-related risk factor for Fas -670G to A SNP and T allele-related risk factor for FasL -844C to T SNP with the incidence of severe oligozoospermia in Iraqi patients.


Article
InVitro: Detection TaSOS1 gene in four Iraq genotypes of Bread Wheat under different salt stress levels.

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Abstract

In this study, Four Iraqi genotypes of Bread Wheat (Iraq, 2H,3H and Hussein) were used to detection (TaSOS1) gene (a transmembrane Na+/H+ antiporter) under salt stress with different concentrations of NaCl (4,8,12 and 16 ds.m-1) by using PCR technique. The results showed that Iraq cultivar was sensitive to salinity, It gave less values in all trait studies. 2H and Hussein genotypes were salt tolerance, It showed in most trait studies (Callus relative growth , Calli water content , Proliferation efficiency of dry weight, Regeneration frequency , shoot length and K+/Na+ ) , 3H genotypes was salt tolerance but less degree from 2H and Hussein. The detection on gene by using PCR technique , Gene was shown in three genotypes (2H,3H and Hussein) but it was not found in Iraq . So this gene was found important to salt tolerance .


Article
Molecular Diagnosis of Chlamydia pneumoniae in Coronary Heart disease Patients in Diyala City

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Abstract

Chlamydia pneumoniae has been highly associated with & implicated in the procoagulatory & inflammatory component of atherosclerosis. The present study has been designed to detect C. pneumoniae DNA and C. pneumoniae IgG and IgM antibodies among Iraqi patients with coronary heart disease and the association between the bacterium and risk factors. The study involved 71 patients diagnosed with (angina, myocardial infarction and atherosclerosis) and 30 healthy controls. Out from 71 patients, 28.2% were smoking, 26.8% were diabetic and 45.1% were hypertensive. Detection of anti- C. pneumoniae IgM and IgG antibodies was done by Enzyme-Linked Immunosorbent assay. C. pneumoniae IgG antibodies were detected in 57.7% of patients and 14.6% of healthy controls, while seropositivity of C.pneumoniae IgM antibodies were detected in 9.9% patients. DNA were extracted from blood samples and PCR was used for detection of the C. pneumoniae genes. Thirty DNA samples of the coronary heart disease patients were amplified by PCR using three genes 16SrRNA with 194bp and outer membrane protein (OMP) and were included as internal with 239bp and outer with 499bp both specific gene for C. pneumoniae. The results of all genes revealed that 10 patients (33.3%) were positive , while it was negative for all control group.

Keywords

C. pneumoniae --- CHD --- Molecular detection --- PCR.


Article
Biofilm Formation and Detection of pslÁ Gene in Multidrug Resistant Pseudomonas aeruginosa Isolated from Thi-Qar, Iraq

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Abstract

Pseudomonas aeruginosa is considered as one of the major causes of hospital-acquired infections due to its high antibacterial resistance. Biofilm formation is a well-known pathogenic mechanism in P. aeruginosa infections. The detection of polysaccharide pslÁ gene can be important for biofilm formation by this bacteria. Therefore; A total of 96 swab sample were collected from burn and otitis infections at the two hospitals in THi_Qar province, Iraq, there were just 93 samples gave positive growth. By differential and selective media(McChonky agar,Cetrimide agar at 44oC, King A agar), biochemical tests(gram staining, oxidase, triple sugar iron agar and others) and growth at 42oC to detection and diagnosis the isolates as Pseudomonas species, followed by APi 20E assay supported by genotypic determined of 16S rRNA which proved there were 37(39.7%) identical isolates as P. aeruginosa. Antibiograms susceptibility test was performed via agar disk diffusion method were isolates subjected to 12 different items of antibiotics appeared that 12 and 4 isolates were multidrug resistant of burns and otitis respectively, with 3 isolates were pan-drugs resistance from burns. The ability of producing biofilm was examined by two phenotypic methods were Congo Red Agar and Crystal Violet Microtiter Plate Assay, the results showed that 32/37 (86.49%) isolates were slim adhesion formed in Congo red test, also there were 31/37 (83.78%) isolates produced biofilm formation, there were 43.47% and 21.42% strong biofilm formation from burns and otitis respectively by second method, all strong biofilm formation isolates were multidrugs resistance isolates. The detection and prevalence of the pslÁ genes among the isolates was determined by conventional monoplex polymerase chain reaction (PCR), the outcome exhibited that 34/37(91.89%) isolates carriage the pslÁ gene, also one and two from burns and otitis isolates respectively didn't carry this gene. The recent report is the first study with biofilm pslÁ gene in Iraq, and concluded that pslÁ gene harbored in most P.aeruginosa isolates even strong, moderate and weak biofilm but in different grade that mean there are another genes can contribute in biofilm synthesis must be investigated.


Article
Correlation between vitamin D receptor gene polymorphisms and levels of some hormones in Iraqi infertile women with polycystic ovary syndrome

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Abstract

The polycystic ovary syndrome (PCOS) is a disorder that characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphologic features. As defined by the diagnostic criteria of the National Institutes of Health (i.e., hyperandrogenism plus ovulatory dysfunction). The purpose of this study was to investigate the correlation between VDR gene polymorphisms and level of LH, FSH, TSH and Prolactin hormones .This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from November 2016 - August 2017, The PCOS patients were taken from the Kamal Al-Samarraee Infertility Treatment Hospital in Baghdad.Women with PCOS (n=50) and apparently healthy control group (n=50) , were enrolled . Genotyping of VDR gene (rs2228570) (rs7975232) , as well as (rs731236) SNPS between groups were determined by using Taqman genotyping assay . Hormonal analysis for LH, FSH, TSH and Prolactin was performed by using Automated Immune Assay (AIA). the results of the present study indicate that serum LH , FSH and TSH concentrations were unaffected by the studied SNPs of VDR gene within carriers of genotypes of rs2228570 , rs7975232 and rs731236 SNPs in VDR gene, while serum prolactin levels were significantly (p<0.05) higher in PCOS patients versus controls.

Keywords

PCOS --- VDR --- Polymorphism --- Infertile.

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