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Article
Sequencing characterization of housekeeping genes among Klebsiella pneumoniae isolated from burn patients

Authors: Abbas Atyia Hammoudi --- Azhar N. Hussein
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 111-119
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Burn wound infections are one of the most important impairments that occur in the acute period following injury and colonization by the pathogenic agents, including gram-positive, gram-negative bacteria and yeasts. The study included 210 clinical swab samples have been collected from burn- wound patients and cultured on blood agar, MacConkey agar and Eosin methylene blue agar, the period from 1/3/2016 to 30/8/2016 at different hospitals in Baghdad city. By microscopic characterizations, morphological and biochemical reactions, the results showed that 42 (37.5 %) isolates belong to Klebsiella pneumoniae. The analysis of (10) clinical origin of Klebsiella pneumoniae isolates by multilocus sequence typing show the relationship between the local and global isolates which belonged to 7 housekeeping genes (rpoB;beta-subunit of RNA polymerase, gapA; glyceraldehyde 3-phosphate dehydrogenase, mdh; malate dehydrogenase, pgi; phosphoglucose isomerase, phoE; phosphorine E, infB; translation initiation factor 2, tonB; periplasmic energy transducer). The present study the results showed the 10 isolates of K. pneumoniae were identified into different sequence type (ST): ST 14 and 15 for (IQK1, IQK2, IQK3, IQK4, IQK5, IQK6 and IQK7), ST 266, 54, 709, 728 and 1177 for (IQK8 and IQK9) and ST 665, 975 and 2149 for (IQK10). In addition, the result showed 100% identities with previously reported genes. There was no information on the sequence type (ST) (an allelic profile) of K. pneumoniae in Iraq. According to the results of the present study, the most occurrence clones found in Baghdad hospitals were endemic ST14 and 15, which accounted for 70% of the isolates (n=10). The presence of the ST14 and 15 clones in Iraq which came closer to global (14 and 15 STs) clones might be indicating intercontinental transmission because these clones were added to the list of the strains that isolated from different countries.

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Article
Curing of plasmid contents of Proteus spp. isolated from urinary tract infections in AL- Diwaniyah city

Authors: Ali A. AL-Nashaa' --- Azhar N. Hussein --- Adnan H. AL-.Hamadan
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2007 Volume: 2 Issue: 3 Pages: 160-169
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

This study included isolation and identification of Proteus spp . from urinary tract infections of Gynecology and padiatrics Hospital teaching in Diwaniyah cityand investigated the virulence factors connected with pathogenicity of this bacteria. It aims also at curing of plasmid contact by means of using some chemical materials. The bacteria Proteus spp. Isolates formed 16% which belong P. mirabilis and P. vulgaris from bacterial causes of urinary tract infection in children. The isolates of this bacteria has some physiological characteristics which represent virulence factors related to the ability of bacteria to make infection .It seems that 80% from these isolates were capable to Haemagglutination of RBCs . These isolates produce also Haemolysine enzyme. It was found that percentage of production was 60% . As for B- lactamase enzyme production 40% of these bacteria isolates showed ability to produce his enzyme. Moreover, only 20% from Proteus isolates showed ability to produce bacteriocin production. All these isolates were not able to produce capsule production.When testing the resistance of Proteus isolates toward 8 different commonly used antibiotics in treatment of urinary tract infection, results showed that the isolates have different resistance depending on the type of antibiotic and the strain of the isolates. All these isolates were completely resistance Nitrofurantion in 100% while the least resistance toward Nalidixic acid and Gentamicin reached 20% for both.The results showed that there were DNA plasmid in 3 bacteria isolates but there was no relationship between the plasmid bands and resistance toward antibiotics. The P. mirabilis isolate which contained 4 plasmid bands was treated with SDS and Ethidiun bromide in an attempt to curing plasmid bands. The result showed that this isolate lost the ability to produce the majority of virulence factors that caused this disease and lost its resistance for som antibiotics especially Nalidix acid , Ampicillin and Choramphenicol. The ethidium bromide was the most effective in curing process since the resultant strain aest all plasmid bands.

شملت الدراسة عزل وتشخيص بكتريا Proteus spp. من اخماج المسالك البولية من الأطفال المراجعين لمستشفى النسائية والأطفال في مدينة الديوانية، والتحري عن عوامل الضراوة المرتبطة بامراضية هذه البكتريا، والعمل على تحييد المحتوى البلازميدي باستخدام بعض المواد الكيميائية. شكلت عزلات بكتريا Proteus Spp. 16% والعائدة للنوعين P. mirabilis و P. vulgaris من المسببات الجرثومية لخمج المسالك البولية في الأطفال . تميزت عزلات هذه البكتريا بمجموعة من الخصائص الفسلجية التي تمثل عوامل ضراوة ترتبط بقدرة البكتريا على أحداث الخمج حيث أظهرت 80% من عزلاتها قدرة على تلازن كريات الدم الحمر، كما كانت هذه العزلات منتجة لأنزيم الهيمولايسين الذي يحلل الدم وبنسبة 60%، أما أنتاج أنزيمات البيتالاكتاميز فقد أظهرت 40% من عزلات هذه البكتريا قدرتها على إنتاج هذه الأنزيمات . ولم تظهر ألا 20% من عزلات بكتريا Proteus spp قدرتها على أنتاج البكتريوسين، كانت جميع هذه العزلات غير قادرة على تصنيع المحفظة. عند اختبار مقاومة عزلات البكتريا Proteus spp تجاه ثمانية مضادات شائعة الاستخدام في علاج اخماج المسالك البولية، أظهرت عزلاتها مقاومات متفاوتة حسب طبيعة المضاد ونوع العزلة، فقد كانت جميع العزلات مقاومة للمضاد Nitrofurantion وبنسبة 100% بينما أظهرت أدنى مقاومة تجاه المضادين Nalidixic acid و Gentamicin بلغت 20% لكليهما. ظهر وجود الدنا البلازميدي في ثلاثة عزلات بكتيرية، لكن لوحظ عدم وجود علاقة بين عدد الحزم البلازميدية التي تمتلكها العزلة وعدد المضادات التي قاومتها . عوملت العزلة P. mirabilis التي تحتوي على أربع حزم بلازميدية بمادتيSDS وبروميد الاثيديوم في محاولة لتحييد محتواها البلازميدي، وقد تبين أن هذه العزلة فقدت قدرتها لإنتاج معظم عوامل الضراوة المسببة للمرض، كما أنها فقدت مقاومتها لبعض مضادات الحياة خاصة Nalidixic acid و Ampicillin و Chloralmphnicol وكانت مادة بروميد الاثيديوم هي الأكثر فعالية في عملية التحييد، إذ فقدت السلالة الناتجة جميع حزمها البلازميدية.

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