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Molecular detection of some virulence gene associated with pathogenicity of Klebsiella pneumoniae


In this study, only seven isolates of Klebsiella pneumoniae were subjected for detection espB, cesT/eae, and CNF1 genes in their genome.It was seen that esp gene was not found in all isolates understudy, whereas cytotoxic necrotizing factor-1(cnf1) was detected in four isolates of Klebsiella pneumoniae. Moreover, three isolates were shown to possess cesT/eae gene in their genome. The presence of such genes will provide the bacteria to invade and evade the host immune system and also will interfere with wound healing as a result of cnf1 production.

:- حيث تم التحري على احتواء Klebsiella pneumoniae في هذه الدراسة, تم عزل وتشخيص سبع عزلات من بكتريا العزلات للجينات espB, cesT/eae, and cnf1 . وقد وجد ان جميع العزلات غير حاوية على espB في حين وجد ان ثلاث عزلات فقط حاوية على cesT/eae . كما وجد ان اربع عزلات فقط حاوية على cnf1 جين. كما لوحظ ان وجود هذه الجينات في البكتريا يزيد من قابليتها على غزو وتجنب الجهاز المناعي ويزيد من قابليتها على حدوث الاصابة وبالأخص وجود جينات cnf1.


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