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Article
The relationship between bcl-2, and p53 protiens in transitional cell carcinoma of the bladder
العلاقة بين بروتين Bcl- 2 و P53 في امراضية سرطان المثانة الانتقالي

Authors: Amina Y. Abd Ullateef أمنة يوسف --- Dina W. Abed دينا وائل --- Wasan A. Bakir وسن عبد الاله
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2011 Volume: 4 Issue: 1 Pages: 1-6
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Transitional cell carcinoma (TCC) of the bladder in patients has historically a favorable prognosis. Bcl-2 and p53genes are implicated in cell cycle regulation with roles on programmed cell death. Presence of nuclear accumulationof p53 and cytoplasmic accumulation of bcl-2 were proposed to confer a growth advantage to tumor cells. In this study,we investigated the roles of p53 and bcl-2 as prognostic factors in TCC of bladder.Method: Investigated 25 patients with transitional cell carcinoma (TCC) and 27 patients with cystitis (control group) forthe expression of Bcl-2 and P53 by immunohistochemical staining with specific monoclonal antibodies.Results: Bcl-2 was significantly expressed in TCC compared to those with cystitis (control). Moreover, P53 was significantlyexpressed in TCC compared to those with cystitis (control). A significant positive correlation between these twoparameters in Tcc.Conclusions: These observations detect that increased the expression of Bcl-2 and P53 in TCC play a potential role inthe development of bladder cancer.

الهدف : قياس مستوى تعبير بروتين Bcl-2 وp53 لمرضى سرطان المثانة الانتقالي .
الطريقة : استخدمت طريقة التحليل الكيميائي المناعي لكشف وتحديد مستوى بروتين Bcl-2 ,p53 في المقاطع النسيجية المطمورة في البرافين والتي تم الحصول عليها من 25 مريضا مصابا بسرطان المثانة الانتقالي Tcc )) والمجموعة الثانية تشمل 27 مريضا مصابا بالتهاب المثانة حيث اعتبروا محموعة سيطرة. النتائج : التحليل الكيميائي المناعي لمستوى بروتين Bcl-2 يزداد زيادة معنوية في حالة سرطان المثانة الانتقالي عن مجموعة السيطرة . ومستوى تعبير بروتين الp53 يزداد ايضا معنويا مقارنة مجموعة السيطرة .وهناك ارتباط موجب بين BCL-2 و P53 .
الاستنتاج : بروتينات لBcl-2 ,P53 تلعب دورا مهما في مرضى سرطان المثانة الانتقالي وان تعبير Bcl-2 يرتبط ارتباطا موجبا مع تعبير 53P .

Keywords

Bcl-2 --- P53 --- TCC --- IHC. --- Bcl-2 --- P53 --- TCC --- IHC.


Article
Prognostic Significance of Immunohistochemical Overexpression of P53 And Bcl-2 Proteins in Patients with Non-Hodgkin’sLymphoma

Authors: Farah Amer Albejow --- Hadeel Abdul-Elah Kerbal --- Najlaa Bader Alawadi --- Ahmed Raji Kharulla
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 721-729
Publisher: Babylon University جامعة بابل

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Abstract

Non-Hodgkin's lymphomas are the hematologic malignancy with the most prevalence worldwide, they are generally classified as B-cell or T-cell lymphoma .Theincidence rate of NHL for the total population is 5.16 % according to Iraqi cancer registry (ICR) in 2010.This study was designed to asses’bcl-2 and p53 overexpression in Iraqi patients with NHL and correlation with different prognostic indices (age, sex, stage, grade, and International prognostic index).Samples included in this study represented as 50 formalin-fixed paraffin embedded lymph node blocks of patients with NHL,during the period from September 2013 till November 2014,and these blocks collected from the histopathology laboratories of Al-Hilla Teaching Hospital as well as many private histopathology laboratories in Hilla city, These blocks stained Immunohistochemically for bcl-2 and p53 protein expression by using L-SAB+ staining methods.Results revealed Significant correlation between bcl-2, p53overexpression and different grades of NHL (p-value < 0.05), while there are no significant relationship between bcl-2, p53 and age, sex, stage, and IPI score (p-value > 0.05).In conclusion there are up regulation of P53 protein associated with down regulation of bcl2 protein in patients with high, intermediate to low grades of NHL while no significant association with IPI score& factors.

Keywords

Non-Hodgkin lymphoma --- P53 --- Bcl-2


Article
Protective Role Of Honey on The Dorsal Surface of The Tongue of Methotrexate Treated Rats (Histological and Immunohistochemical Study)

Authors: Ali Sultan Al-Refai --- Hassan Ali Al-Barazenchy --- Hanan Abdulla Abdulqader --- Ameera Kamal Khalil
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 3 Pages: 670 -678
Publisher: Babylon University جامعة بابل

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Abstract

The most common side effect of chemotherapy is oral mucositis and the effective treatment is considered important in cancer patient. This study was aimed to evaluate the effect of honey as a treatment for dorsaltongue mucositis caused by the methotrexate.Forty Albino rats were usedin the present study and grouped randomly into control and study groups(20 animals each). The rats in the control group were divided randomly into two groups: 10 animals were treated by distilled water and 10 animals were treated by (2.5gm/kg) honey two times daily for eight days using gavage needle, and a physiological salinewas intraperitoneally injected at day four for all the animals. In the study group, they were intraperitoneally injectedby60 mg/kg of methotrexate at day four and the animals were treated in the same way like control group. At day eight, all the animals were sacrificed;and a cross section fromthe tongue was removed for histopathological and immunohistochemical (Ki-67 and Bcl-2 immunolabeling) analysis.Honey can partially protect the tongue from methotrexate induced cytotoxicity, and attenuate the associated injury. Ki-67 immune expression was non significantly increased (p>0.05), but Bcl-2 immune expression was significantly increased in (p<0.05) in comparison with methotrexate treated group.Honeycan produce protection against methotrexate induced dorsal tongue mucositis.Flavonoids and phenolic compounds in honey can accelerate the healing process by its anti-oxidant and free radical scavenging properties.

Keywords

Honey --- Mucositis --- Chemotherapy --- ki-67 --- Bcl-2


Article
THE POSSIBLE PROTECTIVE ROLE OF BCL-2 IN RECURRENT ABORTION
الدور المحتمل لبروتين (BCL-2) في الحماية من الإجهاض المتكرر

Author: Haider Sabah Kadhim حيدر صباح كاظم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2010 Volume: 8 Issue: 3 Pages: 49-54
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Recurrent abortion is a worldwide problem, with undefined causes. Apoptosis could play a major role in the process.Objective: Detect the expression of Bcl-2 protein at the materno-fetal interface in patients with recurrent pregnancy loss (RPL).Methods: Immunohistochemistry analysis of Bcl-2 protein using paraffin embedded sections of curate samples obtained from 40 women divided into three groups: 24 women with recurrent abortion, 10 women with abortion for the first time, and 6 women with induced abortion.Results: The mean value of the expression of Bcl-2 protein was (57.9± 1.4), which is significantly higher than that of the second group (39± 1.9), and the third group (47.5± 2.4).Conclusion: High expression of Bcl-2 protein in women with recurrent abortion may have a protective role in preventing placental apoptosis that leads to failure of pregnancy. Keywords: Bcl-2, recurrent pregnancy loss (RPL).

خلفية الدراسة: الإجهاض المتكرر عند النساء الحوامل يعتبر معضلة طبية في كل إنحاء العالم. هدف الدراسة: التحديد الموضعي وتقييم بروتين (BCL-2) في حالات فقدان الحمل المتكرر.المرضى وطريقة الدراسة: استخدمت تقنية التصبيغ الكيميائي النسيجي المناعي لبروتين Bcl-2 في عينات الجرف الرحمي والتي تم الحصول عليها من 40 امرأة تم تقسيمهن إلى ثلاث مجاميع:المجموعة A وتشمل 24 امرأة حصل لها فقدان حمل متكرر،المجموعة B وتشمل 10 نساء حصل لهن إجهاض تلقائي للمرة الأولى، والمجموعة C وتشمل ستة نساء أجري لهن عملية إنهاء حمل علاجي.النتائج: أظهرت النتائج إن مستويات التعبير الموضعي بروتين (BCL-2) في حالات فقدان الحمل المتكرر ذات زيادة ملحوظة مقارنة مع المجموعتين الثانية والثالثة.الاستنتاج: إن ارتفاع نسبة التعبير الموضعي لبروتين (BCL-2) قد يلعب دور مهم في الحماية من موت الخلايا المبرمج في الرحم مما يؤدي إلى فشل الحمل.مفتاح الكلمات: بروتين BCL-2, الإجهاض المتكرر


Article
Immunohitochemical expression of Bcl-2in human colocrectal carcinoma
التعبير المناعي النسيجي للمعلم السرطاني ال(bcl-2 ) في سرطان القولون والمستقيم

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Abstract Background: Colorectal carcinoma is the most frequent type of malignancy in terms of incidence,it is the third most common cancer world wide in both sexes . bcl_2 expression has been described as been a better prognostic factor in colorectal carcinoma .Objcetives:-To determine the frequency and the pattern of Bcl-2 expression in colorectal carcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.Material and method: -Thirty cases of colorectal carcinoma were studied, these cases were diagnosed in private laboratories in Baghdad / Iraq from January 2015 to Juan 2015. Clinicopathological parameter such as age, gender , pathological diagnosis , tumor site , lymph nodes status , grade and stage of tumor were collected from patients files..Sections of 4µm were stained by hematoxylen and eosin stain, and immunohistochemical stained for Bcl-2.Results:-Nineteen (63.3%) cases were males, 11(36.7%) cases were females, with age distribution ranging from (39-89) years with a mean age of 56.5 years. Histologically the tumor grade range from moderately differentiated in 27 (90%) cases and poorly differentiated in 3 (10%) cases. Ten (33.3%)cases located in the right colon and sigmoid each 6(20%) cases in rectum,8(26.7%) cases in the cecum,3(10%)cases located in right colon and sigmoid each,6(20%) cases in the rectum and 8(26.7%) cases in the left colon. Regarding pathological staging (TNM system),2 (6.7%) cases were stage T2 ,24 (80%) were stage T3 ,4(13.3%) cases were T4. Lymph node involvement found in 14(46%) cases and distant metastasis was found in 9(30%) cases. Bcl-2 expression was present in 14(46.7 %) cases of 30 colorectal carcinoma.There was no significant correlation with age, sex, histopathological grade, location, lymph nodes status and tumor invation.Conclusion:-There was no significant statistical correlation between bcl expression by tumor cell of colorectal carcinoma and different clinicopathological parameters in this study.Keywords: Colorectal cancer, Bcl-2 expression

المقدمه:يعتبرسرطان القولون في معظم الأحيان هو اكثر أنواع الأورام الخبيثة من حيث الإصابة، هو ثالث أكثر أنواع السرطان شيوعا في جميع أنحاء العالم في كلا الجنسين.المعلم السرطاني bcl-2 هوالافضل في حالة سرطان القولون والمستقيم.الهدف من الدراسة: -تحديد نسبه وشكل التعبير المناعي ل bcl-2 في سرطان القولون والمستقيم بواسطه الفحوص المناعيه والنسيجيه، وتقييم العلاقه بين التعبير المناعي مع مختلف الدلالات السريريه والمرضيه. المواد والأسلوب: -ثلاثون حاله لمرضى سرطان القولون والمستقيم، تم دراستها وهذه الحالات تم تشخيصها في المختبرات الاهليه في بغداد / العراق خلال الفترة من كانون الثاني2015 لغايه حزيران2015. هذه الحالات تم تقيمها من ناحيه العمر والجنس والتشخيص النسيجي، وتشمل حجم الورم, موقع الورم ، عدد الغدد الليمفاوية ، تمايز الورم ومراحل انتشار المرضتم تقييم التعبير المناعي ل bcl-2لجميع الحالات بواسطه فحوصات المناعه النسيجيه الكيميائيه.النتائج:- تسعه عشر(63.3%) من الحالات كانوا ذكورا، و 11 (36.7%) من الحالات من الإناث، باعمار تتراوح من (39-89) سنة بمتوسط عمر 56.5 عاما. نسيجا تتراوح درجة التمييز بين معتدلة في 27 (90%) من الحالات ومتباينة سيئة في 3 (10%) من الحالات. عشرة (33.3%) من الحالات في القولون الأيمن والسيني و6 (20٪) من الحالات في المستقيم، 8 (26.7%) من الحالات في الأعور، 3 (10%) من الحالات تقع في القولون الأيمن والسيني و6 (20 ٪) من الحالات في المستقيم و8 (26.7٪) من الحالات في القولون الأيسر. وفيما يتعلق بمراحل المرض كانت 2 (6.7٪) من الحالات كانت في مرحلة الثانيه 24(80%) في المرحلة الثالثه، وكانت 4 (13.3%) من الحالات في المرحله الرابعه. اما حالات العقدة الليمفاوية الموجودة في 14 (46٪) من الحالات والانبثاث البعيدة في 9 (30٪) من الحالات. كان التعبير المناعي النسيجي bcl-2 موجودة في 14 (46.7٪) من حالات القولون والمستقيم 3 من اصل الثلاثون حاله سرطانيه0 ليست هناك علاقه بين التقدم في العمر، والجنس، الصف الأنسجة، والموقع، الغدد الليمفاوية والتعبير المناعي.الاستنتاج:- بالرجوع الى البيانات التي تخص التعبير المناعي لBcl-2 في سرطانات الفولون تبين انه لاتوجد علاقه احصائيه بين التعبير المناعي لBcl-2 مع مختلف الدلالات السريريه والمرضيه.كلمات مفتاحيه:- سرطان القولون والمستقيم, التعبير المناعي Bcl-2


Article
mRNA in situ hybridization analysis of p-53 cancer suppression gene and Bcl-2 oncogene in chronic lymphocytic leukemia

Authors: Saad A. Mustafa --- Haythem A. Al-Rubaie --- Abdulkareem M. Jaafar
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 2 Pages: 177-181
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Several factors render chronic lymphocytic leukemia (CLL) an interesting subject for study by researchers. These include marked progress in understanding the molecular biology of normal and neoplastic lymphocytes and recent advances in molecular genetics techniques. Among molecular markers, p53 cancer suppressor gene and the antiapoptotic gene Bcl-2 have been widely studied. Patients and methods: A retrospective cross-sectional study done on 60 patients with chronic lymphocytic leukemia compared with 20 controls (anemic patients), all recruited at the Medical City Teaching Hospital laboratories from January 2004 to December 2007. The bone marrow biopsy of each was re-examined histologically. In situ hybridization was performed utilizing biotin labeled p53 and Bcl-2 cDNA probes.
Results: The frequency of p53 positive signals in the study group was 28.3% (17of 60 cases). A significantly larger number of patients, with high score for p53 signal, were associated with high-risk clinical stage than patients with low score (p = 0.005). There was a significant direct positive correlation between increasing scores of p53-positive chronic lymphocytic leukemia cells and advancing clinical stage of the disease (p =0.002).
The frequency of Bcl-2 positivity was 50% (30 of 60 cases). No significant correlations found between Bcl-2 scores and clinical stage of the disease.
Conclusion: Although p53 alteration may occur early in the course of the disease, as shown by the p53 positivity in a proportion of patients in low and intermediate-risk stage of the disease, the highest frequency p53-positive cells, has been observed in high-risk stage of the disease. Therefore, p-53 score is an important prognostic variable in patients with chronic lymphocytic leukemia. However, Bcl-2, as assessed by ISH, is not regarded as an important prognostic marker.


Article
BCL-2 OVEREXPRESSION IN COLORECTAL CARCINOMA
فرط التعبير المناعي للـ Bcl-2 في سرطان القولون والمستقيم

Author: Alaa Ghani Hussain علاء غني حسين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2008 Volume: 6 Issue: 2 Pages: 66-76
Publisher: Al-Nahrain University جامعة النهرين

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Background: Colorectal cancer is a major cause of morbidity and mortality worldwide. Prognostic assessment influences the treatment of patients with colorectal cancer, including decisions about adjuvant therapy. Bcl2 over-expression is a genetic event associated with tumor progression and is a prognostic marker of this disease.Objective: Colorectal carcinoma is a major cause of morbidity and mortality worldwide. Bcl2 overexpression is a genetic event associated with tumor progression and is a prognostic marker for this disease. The aim of this study is to assess the expression of bcl2 in colorectal carcinoma and its correlation with other clinicopathological parameters.Methods: From January 2004- January 2005, thirty –five formalin fixed paraffin embedded tissue samples from patients with colorectal carcinoma were included in this study. Four-micrometer tissue sections were obtained for each case, two of them were stained by H&E and the diagnosis had been revised, and the other two were stained immunohistochemically by using avidin biotin alkaline phosphatase method for evaluating bcl2 expression. The presence of red cytoplasmic staining in less than 25% of tumor cells was considered a positive expression of bcl2.Statistical analysis of all the results were performed using Chi square test at level of significance alpha = 0.05 (P<0.05) regarded as statistically significant.Results: Bcl2 expression was significantly higher in low grade and early stage colorectal carcinoma .Non mucinous colorectal cancer showed more bcl2 expression than the mucinous type. An inverse correlation was found between bcl2 expression with the greatest diameter of the tumor and the lymph node status. Bcl2 expression was correlated neither with the age nor with the sex of the patient and the tumor location.Conclusion: Bcl2 over-expression correlates with many variables as low grade colorectal tumor, early stage, non mucinous type, small tumor size and negative lymph node status.

خلفية الدراسة: يعد سرطان القولون والمستقيم سبب رئيسي للفنائية والمرضية في جميع انحاء العالم . ان التقييم التكهني يؤثر على علاج سرطان القولون والمستقيم بضمنها القرارات حول العلاج المساعد .يعد التعبير المناعي للـ bcl2 هو حدث جيني مرتبط بتقدم الورم وهو مؤشر تكهني للمرض.هدف الدراسة: دراسة التعبير المناعي للـ bcl2 في سرطان القولون والمستقيم وبيان مدى علاقته بالعوامل المرضية والسريرية للورم.المواد وطرق العمل: للفترة من كانون الثاني 2004 – كانون الثاني 2005 تم اختيار 35 مقطع شمعي لمرضى مصابين بسرطان القولون استعاديا تغطي الفترة من كانون الثاني 2004 الى كانون الثاني 2005 وتم تحضير اربعة شرائح نسيجية بسمك 4 مايكروميتر, اثنان منها صبغت بصبغة الهيماتوكسيلين والايوسين وتم اعادة فحصها لغرض تشخيصها وتصنيفها نسيجيا والاثنان الاخران صبغت بطريقة التصبيغ الكيميائي النسيجي المناعي للكشف عن الـ bcl2 . ان وجود التفاعل السايتوبلازمي الاحمر في المقطع اعتبر تفاعلا ايجابيا . بدمج الشدة والكمية للصبغة المناعية فان الصبغة السايتوبلازمية الضعيفة مع كمية من الخلايا الورمية اقل من 25 % اعتبر ايجابيا للتعبير المناعي للـ bcl2 .تم التحليل الاحصائي لكل النتائج بأستعمال Chi square واعتبر ت قيمة P اقل من (0,05) كقيمة ذات مغزى او دلالة احصائيا.النتائــج: ان التعبير المناعي للـ bcl2 في الدرجة الدنيا كان ذا مغزى احصائيا . وجد ان تعبير ال bcl2 كان اكثر في الاورام عالية التمايز النسيجي (او ذات الدرجة الدنيا لتصنيف المرض ) وفي المراحل المبكرة للورم . اظهر سرطان القولون والمستقيم غير المخاطي الـ bcl2 اكثر من النوع المخاطي وكان هذا ذو قيمة او دلالة احصائيا. هناك علاقة معكوسة وجدت بين التعبير المناعي للـ bcl2 مع اكبر قطر للورم وحالة العقد اللمفاوية . ان التعبير المناعي للـbcl2 غيرمرتبط بعمر وجنس المريض وموقع الورم.الاستنتاج: ان ظهور بروتين bcl2 في سرطان القولون والمستقيم يترافق مع بعض عوامل المرض كالدرجة الدنيا لتصنيف المرض , المراحل المبكرة للمرض , النوع غير المخاطي لسرطان القولون والمستقيم , صغر حجم الورم والحالة السلبية للعقد اللمفاوية .


Article
Immunohistochemical Expression of P53 and bcl-2 in benign and malignant salivary glands tumors

Authors: Dr. Husam Omer Mohammed M.Sc ** د. حسام عمر --- Dr. Jamal N. Ahmed M.Sc* د. جمال احمد --- Dr. Natheer H. AL-Rawi M.Sc, Ph.D * د.نذير الراوي
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2007 Volume: 4 Issue: 1 Pages: 13-21
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Objectives: Salivary glands tumors are relatively uncommon neoplasm with widely variable histopathologic and biologic characteristics. Alteration in some proto-oncogenes and tumor suppressor genes may lead to the development and progression of these tumors. The purpose of the present study was to analyze the immunohistochemical expression of P53 and bcl-2 in some salivary gland tumors in relation to tumor size, histological grade and extent of invasion.Study Design: The series consisted of 22 formalin-fixed paraffin embedded blocks of salivary glands tumors collected from the files of Oral pathology Department./College of Dentistry/ Baghdad University. The specimens consisted of (10) benign salivary gland tumors ( Pleomorphic Adenomas) and (12) malignant salivary gland tumors [ (2) Carcinoma ex Pleomorphic Adenoma, (3) Muco-Epidermoid Carcinoma, (2) Adenocarcinoma (5) Adenoid cystic carcinoma]. These were analyzed for immunohistochemical detection of P53 and bcl-2 in their cell types.Results: Pleomorphic Adenoma showed negative expression of P53 and bcl-2 in 70% of cases, whereas all malignant salivary glands tumors were positive for P53 and bcl-2. P53 and bcl-2 immune-reactivity were associated with larger tumor size, higher tumor grade and greater extent of invasion without reaching statistical significance level.Conclusion: P53 expression could be used as a useful tumor marker for malignant transformation of Pleomorphic adenoma and bcl-2 expression could be used as a useful monitor for measuring the aggressiveness of these neoplasms.


Article
Etanercept ameliorate cardiac damage and Apoptosis Induced by Myocardial Ischemia/Reperfusion in male mice.

Authors: Najah R. Hadi --- Fadhil G. Yousif --- Maitham G Yousif --- Saif M. Hassan
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 105-118
Publisher: University of Kufa جامعة الكوفة

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Abstract

Ischemia-reperfusion of cardiac tissues may lead to a prominent damage of the cardiomyocyte through either necrosis or apoptosis that seems to be the predominant modes of death during this period. In this study, we investigated the effects of Etanercept in regional ischemia/ reperfusion injury and apoptosis. White albino adult male mice were divided into four groups (six mice per group). Sham group, mice were subjected for all surgical procedure without ligation of the left interior descending coronary artery (LAD). Control group, in which LAD was ligated. Control vehicle and Etanercept treated groups, mice subjected to the surgical procedure with ligation of LAD for 30 minutes followed by 120 minutes reperfusion, mice treated with normal saline (etanercept vehicle) and Etanercept (5 mg/kg, I.P. 5 minute before reperfusion). At the end of reperfusion, cardiac tissue caspase 3 and Bcl-2, as well as plasma cardiac troponin I (cTnI) were measured. It has been found that Etanercept treated group showed significant reduction (P˂0.01) in caspase 3and cTnI but increase (P˂0.01) the level of the Bcl-2 as compare with the control groups. Histopathology study revealed that the treatment with Etanercept significantly (P˂0.01) improved cardiac injury as compared with control groups and the total severity scores showed showed 16.7 % of the group had no damage and 50% had mild cardiac injury and 33.3% had moderate cardiac injury of Etanercept treated group. It is concluded that Etanercept reduces cardiac damage and apoptosis associated with ischemia/reperfusion injury.


Article
The Relationship between Human Papilloma virus (HPV) infection and Gene Expression of miRNA-744, BCL-2, CASPASE-3 genes Associated with Cervical Cancer in Iraqi Patients

Author: Tabark S. Jassim , Abdul Hussein M. AlFaisal
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 30-35
Publisher: Baghdad University جامعة بغداد

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Abstract

: Most types of HPV are harmless and some types are high risk and can cause cervical cancer or abnormal cells in the lining of the cervix that sometimes turn into cancer. A total number of 90 subjects (70 Iraqi patients with cervical dysplasia (abnormalities) and 20 apparently healthy women) were included in this study. The patients and healthy were aged between 25-55 years. The molecular detection in our study showed that 30 of patients were positive to HPV and 40 patients were negative to virus compared to negative results in healthy women. The most common genotypes is HPV-16. Also multiple infections of HPV-HR were observed in the infected women. The correlation between gene expression, histopathology and HPV virus showed a significant increase of folding values of the genes mi-RNA744 and caspase-3 parallel to decrease of folding value of the gene Bcl-2 which indicated that the apoptosis rate was increased with the histopathology grade where the highest level of expression of the genes was in carcinoma. The results also revealed that mi-RNA744 could be up regulator of the gene caspase-3 and down regulator of the gene Bcl-2.

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