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CYTOTOXICITY AND INHIBITORY EFFECT OF PARA-AMINO PHENYL MERCURY(II) ACETATE AGAINST GROWTH OF SOME BACTERIA (IN VITRO)
ألسميه الخلويه والتأثير المثبط للمركب بارا- أمينو فنيل خلات الزئبق ضد نمو بعض انواع البكتيريا (مختبريا).

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Abstract

It was found that 0.1gm of para-aminophenyl mercuric acetate PAPMA dissolved in 10ml of distilled water added in to,Muller-Hinton agar, inhibited the growth of four standard strains bacteria [E.coli ATCC25922, S.aureus ATCC 25923, P.aeruginosa ATCC27853 and S.aureus NCTC6571] and four clinical strains bacteria positive and negative to gram stain [Klebsiella sp.,from blood isolate , E.coli, from stool isolate, Staphylococcus sp., from blood isolate, and Proteus sp.,from urine isolate]. Higher concentrations of PAPMA solution in to the medium inhibited growth of bacteria under study more strongly. The minimal inhibitory concentration (MIC) and cytotoxicity of PAPMA were studied against human blood and it was found that it has no hemolytic in RBCs for human in 1– 5µg/ml. The acute toxicity LD50 of PAPMA was studied and it was about 11.3mg/Kg.

وجد ان 1,. من المركب بارا-أمينوفنيل خلات الزئبق (PAPMA) المذاب في 10ملليلترمن الماء المقطر قد ثبط نمو أربعة سلالات مرجعية: S.aureus NCTC6571 E.coli ATCC25922P.aeruginosa ATCC27853S.aureus ATCC25923 بالاضافه الى أربعة عزلات سريرية: Staphylococcus sp.Klebsiella sp.Proteus sp.E.coliكما وجد ان التراكيز العالية من المركب المضاف الى الوسط الزرعي يؤدي الى تثبيط النمو بشكل اكثر.تمت دراسة التركيز المثبط الادنى وكذلك السمية الخلوية لكريات دم الانسان ووجد انه لايوجد تحلل لكريات الدم عند تراكيز تراوحت بين 1 مايكروغرام/ملليلتر 5 مايكروغرام/ملليلتر ويبدأ التحلل عند تراكيز اعلى ,كما تمت دراسة الجرعة القاتلة الوسطى من المركب فوجد انه 11.3 ملغم/كغم وتعتبر هذه الدراسة هي الخطوة الاولى لدراسة هذا المركب الجديد والذي ممكن الأستفاده منه في المستقبل كدواء....................


Article
Cytotoxicity and Antibacterial Effect of 2-(2-hydroxy naphthylazo) phenyl mercuric chloride and 4-(2-hydroxy naphthylazo) phenyl mercuric chloride against some bacterial isolates in vitro
السمية الخلوية والتأثيرضد الجرثومي لمركبي 2- (2- نفثايل آزو) فينايل كلوريد الزئبق و 4- (2- هايدروكسي نفثايل آزو) فينايل كلوريد الزئبق ضد بعض العزلات الجرثومية خارج الجسم الحي.

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Abstract

The 2-(2-hydroxynaphthylazo) phenyl mercuric chloride and 4-(2-hydroxynaphthylazo) phenyl mercuric chloride were evaluated for their biological activity against standard strains of Staphylococcus aureus ATCC25923 and Ecsherichia coli ATCC 25922. The results showed that there is a potent antibacterial activity for these compounds. Minimal inhibitory concentration was determined for two compounds, it was 20µg/ml, 40µg/ml for Staph. aureus, and 50µg/ml, 60µg/m for E. coli respectively. Cytotoxicity assay was carried out against human red blood corpuscles, the two compounds exhibited a toxic effect in all used concentrations.

قدرت الفعالية البايولوجية لمركبي 2- (2- نفثايل آزو) فينايل كلوريد الزئبق و4- (2- هايدروكسي نفثايل آزو) فينايل كلوريد الزئبق ضد السلالات المرجعية لجرثومتي المكورات العنقودية الذهبية والأشريكية القولونية وأظهرت النتائج الفعالية ضد الجرثومية العالية للمركبين. حدد التركيز المثبط الأدنى للمركبين وكان 20مايكروغرام/مل ،40مايكروغرام/مل لجرثومة المكورات العنقودية الذهبية و50مايكروغرام/مل ،60مايكروغرام/مل لجرثومة الأشريكية القولونية على التوالي ولكلا المركبين. أختبرت السمية الخلوية للمركبين المحضرين ضد كريات الدم الحمراء للأنسان، وأظهر المركبان تأثيرآ سامآ في كافة التراكيز المستخدمة.


Article
Protective effect of Lactobacillus plantarum againstSalmonella typhimuriumin mice

Authors: Zahraa K. Zedan --- Shahlaa M. Salih*
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2010 Volume: 52 Issue: 4 Pages: 475-479
Publisher: Baghdad University جامعة بغداد

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Abstract

Background:Probiotics are defined as living microbial food supplements that beneficially affect the host by improving its intestinal balance. In recent years there is a great interest for viable microorganisms that promote or support a beneficial balance of microbial population of gastrointestinal tract. Probiotics may be consumed in different forms, comprising foods, mainly in fermented state and pharmaceutical products, mainly as capsules or in microencapsulated forms.
Methods:Twelve albino mice were divided into four groups designated as 1, 2, 3 and 4. Each group consists of 3mice, and subject to the followings treatments: Group 1: This group was used as a control. Group 2: This group was dosed with 0.1ml. of 0.5 × 105 cfu/ml of Lactobacillus plantarumculture. Group 3: This group was dosed with 0.1ml. of 0.5 × 105 cfu/ml Salmonella typhimurium culture. Group 4: this group was dosed with 0.1ml of 109cfu/ml Lactobacillus plantarum culture, and infected with 0.1ml of 0.5 × 105cfu/ml culture of Salmonella typhimurium, then histological sections were made from intestine and examined to evaluate protective effect of Lactobacillus plantarum.
Results:Histopathological study exhibited that intestinal sections of mice infected with Salmonella typhimurium showeda necrosis, degenerative changes and inflammatory cells infiltration as compared with normal sections taken from uninfected mice, while treating with Lactobacillus plantarum prevented the histopathological effect of Salmonella typhimurium on mice intestine.Conclusion: Probiotic L. plantarumhad a protective effect on intestine sections of mice infected with Salmonella typhimurium.

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