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Article
Antioxidant activity of Linalool

Authors: Majid S. Jabir --- Ali A. Taha --- Usama I. Sahib
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 1 Part (B) Scientific Pages: 64-67
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

In recent years, Essential oils from their various aromatic plants hadbeen reported to be used in treating of many types of cancer due to their antitumoractivity. In addition, numerous studies had investigated the highest capability ofchemopreventive phytochemicals compound to act as anticancer drugs. In thepresent research, the antioxidant activity of Linalool on free radicals compoundswas studied. The Antioxidant activity was performed using two methods, DPPHand Hydrogen Peroxide (H2O2) Scavenging Capacity. The DPPH scavengingactivity demonstrated that Linalool had antioxidant activity comparing withascorbic acid. Linalool demonstrated moderate antioxidant activity with50.57471% compared with ascorbic acid that showed 86%. Meanwhile, H2O2scavenging capacity methods investigated that Linalool exhibited moderatescavenging activity 56.36% comparing with ascorbic acid that showed 65%. Theresults of this study investigated that the Linalool can be used as easily accessiblesource of natural antioxidants. It can able to be used in the treating several typesof cancers as a result of antioxidant activity of it.


Article
KINETICS OF HYDROGEN SEPARATION FROM REFINERY OFF-GAS

Authors: Abbas Khalaf MOHAMMAD --- Nawras Shareef SABEEH
Journal: Al-Qadisiyah Journal for Engineering Sciences مجلة القادسية للعلوم الهندسية ISSN: 19984456 Year: 2018 Volume: 11 Issue: 3 Pages: 396-409
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Adsorption and desorption kinetic curves for equimolar hydrogen – methane mixture on molecular sieve type 5A were experimentally obtained for pressure range 0.122 – 3.546 MPa.The linear driving force rate expression model was used to simulate the dynamic of adsorption and desorption in adiabatic fixed bed adsorber. The model takes into account the interference effects for non-linear isotherms and non-isothermal system. The equations were solved by backward finite difference method with a fixed gridding technique. The individual mass transfer parameters were obtained by matching the theoretical with the experimental data and found to be equal to 8.510 s-1 and 0.783 s-1 for hydrogen and methane, respectively.The predicted effluent histories were shown to be in close agreement with the experimental data for the system. The lowest relative capacity of the bed for methane was almost approximately 95% of that predicted equilibrium capacity. The predicted temperature profiles tracked the experimental temperature data points, but with higher values. Furthermore, the maximum temperature increasing was observed for the adsorption of methane onto 5A molecular sieve at 35 atmospheres and was recorded as 44 K.


Article
Study the Dye Decolorization of Wastewater Using Bentonite, H2O2, Ultrasonic and Laser Techniques

Authors: A.Z. Mohammed --- A. Sh. Hamadi --- H.A.M. Redha
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 1 Part (A) Engineering Pages: 41-45
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

The bentonite is an adsorbent material that is used frequently in industrial fields. bentonite was used with laser to treat wastewater, when laser is a new technique that has ability to remove the dye. Using of laser as a source to refresh with high efficiency has the important in industrial field.In this study laser diode with power 50 mw, He-Ne laser with power 10 mw and DPSSL with power 500 mw were used with the help of ultrasonic bath and H2O2 to promote the work of the laser. AFM and FTIR testing were made to bentonite to study the characteristics of it. Minitab program was used to facilitate calculations and choose the optimum parameters of experiments. The optimum input conditions of experiments to remove the dye from wastewater were chosen. The best concentration of bentonite is 4 g/l, the best temperature of wastewater is 42◦C, the best time of work is 27 min and the best concentration of H2O2 is 10%. It found from study that the increase of each parameters cause decrease in output concentration of wastewater. The accuracy of work was determined from Minitab program, it was 94.54%.


Article
Background Radio emissions observation at 1.42 GHz

Authors: Kamal M. Abood --- Anmar M. Kitas
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 2A Pages: 786-791
Publisher: Baghdad University جامعة بغداد

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Abstract

In this work, a Radio Emission Background at 1.42 GHz; 21 cm Hydrogen line is carried out by using a 3-meter radio telescope, these telescope is placed on the roof of the building the Astronomy and Space Department at the college of Science, University of Baghdad. Background spectrometry files were received and arranged with a schedule of minimum observation time with span in (GHz). In this work, an observation program was set up to identify some variables related to the telescope and its study, including span, sweep time and central frequency using (MATLAB 2013 software) by reading four files which were selected from the dataset and in different observation times and different span


Article
Effect of Ascorbic Acid (Vitamin C) on H2O2 Induced Oxidative DNA Damage in Human Lymphocytes Estimated by Comet Assay
تأثير حامض الاسكوربك على تدمير الحمض النووي التأكسدي المحث بواسطة بيروكسيد الهيدروجين في الخلايا اللمفاوية البشرية والمقاسة بواسطة فحص المذنب.

Authors: Hanaa Naji Abdullah هناء ناجي عبدالله --- Sura Ali Al-Asadi سرى علي الاسدي --- Mohammed Abduldaim Saleh محمد عبدالدايم صالح
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2018 Volume: 14 Issue: 1 - Part 1 Pages: 204-217
Publisher: Diyala University جامعة ديالى

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Abstract

"Oxidative DNA damage induced by reactive oxygen species and free radicals. Reactive Oxygen Species induced oxidative damage plays a key role in DNA damage". Our study aimed to identify the protective effect of ascorbic acid (AA, vitamin C) against hydrogen peroxide induced oxidative damage in DNA using Comet assay. Lymphocytes pretreated with or without antioxidants, incubated at 37C for 30 minutes, then H2O2 (100μM) was added, and incubated again at 37C for 60 minutes. Viability of cells was detected by trypan blue stain exclusion method. The decrease in viability brought about by H2O2 when the cells incubated for 60 minutes and the viability was present to be 39±3% from 80±4% and it was highly developed by the found of AA at 100 μM which appeared 72 ± 1%. These results indicate that the activity of the ascorbic acid as antioxidantas evidenced by its ability to suppress the oxidative effect against H2O2 and protect the lymphocytes. Estimation of comet tail moment and tail length in human lymphocyte treated with 100μM of hydrogen peroxide as positive control showed that 13± 4.5% of the cells showed no DNA damage while the DNA damage from low to very high damage were 19± 3.5%, 12.2± 2.3%, 14± 3.2% and 37± 3.0%, respectively. In contrast, treatment of the cells with 100 μM H2O2in combination with 10, 25,75 and 100 μM of AA reduced the percentage of DNA damage according to the concentrations used and they were 9± 2.3%, 4.5± 1.6%, 6± 0.5% and 10± 1.4%, respectively. In addition, H2O2 induced DNA damageat percentage of 78% at concentration of 100 μmol/L withoutthe addition of ascorbic acid. while the treatment of human lymphocyte with ascorbic acid was able to reduce oxidative DNA damage by 17% in comparison with control group.

مركبات الاوكسجين الفعالة والجذورالحرة تحث تلف الحمض النووي التأكسدي . تلعب تلك المركبات دور رئيسي في تلف الحمض النووي التأكسدي. هذه الدراسة تهدف الى التعرف على تأثير الوقائي لحمض الاسكوربك ضد بيروكسيد الهيدروجين المحث للتلف التأكسدي للحمض النووي في الخلايا اللمفاوية باستخدام مقايسةالمذنب. تم معاملة الخلايا اللمفاوية مع مضادات الاكسدة او بدونها وحضنت بدرجة حرارة 37 درجة مئوية ولمدة 30 دقيقة, ثم اضيف بيروكيد الهيدروجين وحضنت لمدة 60 دقيقة. تم التحري على حيوية الخلايا بواسطة طريقة صبغة تريبان الازرق. ان انخفاض حيوية الخلايا 39±3 بأضافة بيروكسيد الهيدروجين وحضن الخلايا لمدة 60 دقيقة من 80±4 وهنالك زيادة عالية بوجود حمض الاسكوربك عند 100مايكرومولر تظهر 72±1%. هذه النتائج تظهر نشاط حمض الأسكوربيك كمضادات الأكسدة اظهرت حماية عالية ضد بيروكسيد الهيدروجين. وتم قياس ذيل وطول المذنب في الخلايا اللمفاوية بوجود بيروكسيد الهيدروجين(100مايكرومولر) كسيطرة موجبة واشارت النتائج عدم وجود تحطم للحمض النووي(13±4.5) بينما تباينت نسب تحطم الحمض النووي من الواطىء الى العالي جدا على التوالي(19±3.5% ,12.2±2.3%, 14±3.2%,37 ±3%) على التوالي. بينما عند اضافة حامض الاسكوربك بتراكيز( 100مايكرومولر) الى بيروكسيد الهيدروجين بتركيز(10,25,50,75,100مايكرومولر) اظهرت النتائج انخفاض في نسبة تحطم الدنا على مختلف الدرجات. وان الانخفاض العالي. في حين عند معاملة بيروكسيد الهيدروجين(100مايكرومولر) لتراكيز مختلفة من حمض الاسكوربك(10,25,50,75,100مايكرومولر) و اظهرت انخفاض في نسب تلف الحمض النووي بمختلف الدرجات.وانخفاض عالي لتلف الحمض النووي بتركيز 100مايكرومولر لحمض الاسكوربك من تلف الواطىءالى العالي (9±2.3, 4.5±1.6, 6±0.5, 10±1.4) على التوالي. بالاضافة الى ذلك, بيروكسيد الهيدروجين بتركيز 100 مايكرومولر تحث تلف الحمض النووي بنسبة 78% بدون اضافة حمض الاسكوربك. بينما عند معاملة الخلايا اللمفاوية مع حمض الاسكوربك ينخفض تلف حمض النووي بتركيز 17% مقارنة بمجموعة السيطرة.


Article
Role of Alpha Lipoic Acid in Oxidant /Antioxidant Status and Gene Expression of Glutathione Reductase in Hydrogen Peroxide Exposed Rats: (Part -2)

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Abstract

This study was designated to evaluate the protective role of alpha lipoic acid against oxidative stress resulted by hydrogen peroxide on some oxidants/ antioxidants parameters and gene expression of glutathione peroxidase in adult Wistar rats. Forty adult male rats were randomly divided into four equal groups (10 rats /group) and were handled daily as follows for 56 days : Control group were intubated distal water and received ordinary tap water ; group T1 were intubated 60mg/kg B.W of alpha lipoic acid and received ordinary tap water ; group T2 were received hydrogen peroxide in tap water at concentration of 0.5% , while group T3 were intubated 60mg/kg B.W of alpha lipoic acid and received ordinary tap water containing 0.5% hydrogen peroxide. Fasting blood samples were collected at 0, 28 and 56 days of experimental periods for measurement of serum peroxynitrite and malondialdehyde concentrations, as well catalase activity. Furthermore, gene expression of glutathione reductase in liver was investigated. Administration of 0.5% hydrogen peroxide in drinking water (group T2) manifested a significant elevation in serum peroxynitrite and malondialdehyde with significant decrease in catalase and Glutathione, concentrations. Also, a significant decrease in gene expression of glutathione reductase was observed as compared to other treated groups. Nevertheless, rats in group T3 shows a significantly improvement in oxidant /antioxidant status with increase in folds changes of gene expression of glutathione reductase as compared to control and T2. In conclusion, supplementation of alpha lipoic acid to rats significantly reduced oxidative stress –induced by hydrogen peroxide and caused improvement of gene expression of glutathione reductase in liver via its antioxidant properties.

صممت هذه الدراسة لتقويم الدور الوقائي لحمض الفا ليبويك ضد الكرب التأكسدي المستحدث بإعطاء ببيروكسيد الهيدروجين ) في ماء الشرب للج H2O2( لِ رذان البالغة . أست عم أربعون من الجرذان الذكور البالغه وقسمت عشوائيا إلى أربع مجاميع متساوية يوما على النحو الآتي: مجموعة السيطرة اعطيت الماء المقطر ن 56 وعوملت يوميا ولمدة ع مياه الصنبور؛ مجموعة ً فضلا ملغم /كغم من وزن الجسم من حمض الفا ليبويك مع مياه الصنبور العادية؛ في حين أعطيت 60 المعالجة الأولى فأعطيت ملغم /كغم 60 ٪ ، أما المجموعة الثالثة أعطيت 0.5 المجموعة الثانية ماء الصنبور الحاوي على بيروكسيد الهيدروجين بتركيز ٪ . بعد تصويم الحيوانات 0.5 من وزن الجسم من حمض الفا ليبويك مع ماء الصنبور الحاوي على بيروكسيد الهيدروجين بتركيز يوما من التجربه لتحديد تركيز بيروكسينتريت و مالونديالدهيد و نشاط الكاتالازفي مصل 56 و 28 و 0 جمعت عينات الدم للمدد الدم. فضلا عن ذلك تم حساب التغيير في التعبير الجيني للغلوتاثيون ريدكتديز في الكبد . أظهرت النتائج وجود أرتفاع معنوي في تركيز البيروكسينتريت و مالونديالدهيد مع انخفاض معنوي في الكلوتاثايون و نشاط الكاتالاز في مصل الدم وفي التعبير الجيني مقارنة مع المجاميع الأخرى. ومع ذلك بينت النتائج وجود تحسن معنوي في T2 للغلوتاثيون ريدكتديز في الكبد في المجموعة معايير حالة الكسدة/ مانعات الكسدة في مصل الدم قيد الدراسة في مصل الدم مع زيادة في م مستوى التعبير الجيني للغلوتاثيون ريدكتديز في الكبد في المجموعة المعالجة الثالثة مقارنة مع المجموعتين السيطرة ومجموعة المعالجة الثانية . يستنتج من ذلك أن أضافة حمض الفا ليبويك أدى الى حدوث تحسن معنوي في الأنزيمات المانعه للأكسدة مع زيادة التعبير الجيني للغلوتاثيون ريدكتديز في الجرذان المعرضة للكرب التأكسدي المستحدث بأعطاء ببيروكسيد الهيدروجين بسبب فعاليته المضادة للاكسدة

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