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Article
Actinobacillus actinomycetemcomitans in periodontal infections

Authors: Ghada Y Abdul–Rahman --- Blend S Abdullah
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2006 Volume: 6 Issue: 3 Pages: S79-S83
Publisher: Mosul University جامعة الموصل

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Abstract

To isolate and identify Actino–bacillus actinomycetemcomitans because of its importance in periodontal diseases, to show their incidence in periodontal infections, and to study their important virulence factors. Materials and Methods: The isolating procedure had been done through using the selective Dentaid–1 medium, with anaerobic cultivation at 37 °C for 48 hours, and different microscopic features and biochemical activities were carried out. Also the study included the detection of virulence factors that owned by this type of bacteria through various tests. Results: The rate of infection was 45% in patients suffering from periodontal disease and the isolated bacteria had the ability to adhere to human epithelial cells of the mouth, also had the ability to produce bacteriocins as the substantive factors for the bacterial growth among the closer species of bacteria, and the presence of capsule, which was the most important virulence factor. Susceptibility of the isolated bacteria to selected types of antibiotic showed that 100% of the isolates were sensitive to tetracycline and ciprofloxacin, 100% were resistant to erythromycin and vancomycin. Conclusion: Actinohatillus actinomycetemcomitans was very important periodontal pathogen; having a large number of pathogenic and virulence factors; ciprofloxacin is the drug of choice.


Article
The bactericidal efficiency of Chlorhexidine as an endodontic irrigant

Authors: Abdul–Haq A Suleiman --- Ghada Y Abdul–Rahman --- Wiam M. Al–Ashou
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2006 Volume: 6 Issue: 3 Pages: S71-S78
Publisher: Mosul University جامعة الموصل

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Abstract

to determine the bactericidal efficiency of 0.75% Chlorhexidine in vitro . The time required for this irrigant to start its antimicrobial effect on the selected microorganisms isolated from the infected root canals and unidentified samples taken from root canals with necrotic pulps were evaluated. Materials and Methods: The substantive antimicrobial effect of 0.75% Chlorhexidine in vitro is also considered. Samples taken from 13 teeth with necrotic pulp from patients attended the Department of Conservative Dentistry, College of Dentistry at Mosul University. The turbidity method was applied to determine the antimicrobial effect of 0.75% Chlorhexidine and the combination of 0.5% Chlorhexidine and 0.5% sodium hypochlorite comparing with the antimicrobial effect of 2.5% sodium hypochlorite, the time required for these materials to start their antimicrobial effect on the selected microorganisms was determined using contact test. The antimicrobial effectiveness were evaluated at different time intervals, immediately, 5, 10, 15 minutes after the contact of the microorganisms with the irrigating solutions. Results: Both 0.75% Chlorhexidine and 2.5% sodium hypochlorite are effective on microorganisms collected from root canal. There is no significant difference between Chlorhexidine and sodium hypochlorite in their antimicrobial effect on the anaerobic microorganisms, but Chlorhexidine is more effective on the aerobic microorganisms. Conclusion: Chlorhexidine 0.75% and 2.5% sodium hypochlorite has an immediate effect on the selected microorganisms and unidentified samples from the teeth with necrotic pulps. The combination of Chlorhexidine 0.5% and 0.5% sodium hypochlorite has an effect started after 5 minutes

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