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The Location of Aerobactin Determinants of Uropathogenic E. coli in Association with Hemolysin Production Antibiotic Resistance and Patient Characteristics.

Author: Ibtesam Ghadban Auda
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 2 Pages: 223-229
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Aerobactin, Hemolysin and the resistance to some antibiotics axe important Features ofUropathogenic E. coli (UPEC). The characteristics of patients from which we isolate theUPEC have associate with these Features and their determinants location.AIM:We determined the phenotypic expression and gene location of Aerobactin and phenotypicexpression of Hemolysin among 60 UPEC isolates. We correlated the presence of Aerobactin systemwith antibacterial agents resistance. The expression of Hemolysin and the characteristics of patients.METHODS:Two methods of plasmid curin, sodium dodecyl sulfate and elevated temperature plasmid curing, areused then plasmid extraction and agarose gel electrophoresis are performed to determine thelocation of Aerobactin determinants and the size of Aerobactin plasmids, as well as the location ofthe determinants of some antibiotics resistance which were ampicillin (Am), Tetracycline (Tc),gentamicin (GM), Chloramphenicol (C) and co- trimazole (Co).RESULTS:Aerobactin and hemolysin expression among UPEC isolates were 93.3% and 35% respectively. Theisolates of non compromised patients produce statistically higher rate of expressed hemolysin (90.5%, p<0.01). Plasmid- borne Aerobactin was absent in UPEC isolates of non compromised patients(89.5%, p<0.01). On the contrary compromised patient isolates express plasmid Aerobactin of 59.5%(p<0.02). We also found that Aerobactin determinants are located on a plasmid in compromisedpatient isolates and associated with the absence of chromosomal Hemolysin production (82.9%,p<0.01). Yet, The chromosomal aerobactin is associated with hemolysin production (61.9%,p<0.02). Furthermore, UPEC isolates of compromised patients carry relatively large plasmid ofAerobactin (85.7%. p<0.05) and these large plasmids carry either chloramphenicol (83.3%.p<0.02) orgentamicin determinants (100%. P<0.01) but not co- trimazole, tetracycline or ampicillin.CONCLUSION:The isolates of non compromised patients carry chromosomal Aerobactin and hemolysin. While theisolates of compromised patients express plasmid Aerobactin and do not express chromosomalhemolysin. Aeobactin plasmids are relatively large plasmids and carry either chloramphenicol orgentamicin resistance determinants.

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