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Article
EFFECTS OF ETHANOLIC EXTRACT OF TARAXACUM OFFICINALE LEAVES ON SOME PHYSIOLOGICAL AND REPRODUCTIVE PARAMETERS OF LOCAL ADULT MALE RABBITS

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Abstract

The twenty adult local male rabbits of mean body weight of 1 kgm and mean age of 6 months were randomly allocated into two groups of ten animals, each group was fed during the period of experiment on a standard diet . The first group was regarded as control and received 3ml of physiological saline orally for a month. The second group was drenched ethanolic extract of Taraxacum officinale (500 mg/kg/day) for one month . Those male were joined with females after treatment for a week to study the effect on fertility and reproductive efficiency. The following results were obtained: The ethanolic extract of Taraxacum officinale caused highly significant increase (p<0.01) in AST and significant decrease (P<0.05) in ALT of intact treated male rabbits.The ethanolic extract has a significant decrease in the testicular weight, seminiferous tubules diameter and decrease in sperm count.The prolonged drenching of T.officinale extract caused infertility. Prolonged drenching of T.officinale extract showed clear histological changes on all organs studied (pituitary,thyroid glands and testes) as compared with control

تم أجراء هذه الدراسة على عشرين ذكرا من الأرانب المحلية البالغة الخالية من الأمراض سريريا بمعدل وزن 1كغم ومعدل عمر ستة أشهر، وزعت عشوائياً إلى مجموعتان كل مجموعة احتوت على عشر ذكور. غذيت جميع المجموعات طيلة فترة التجربة على عليقة قياسية . اعتبرت المجموعة الاولى كمجموعة سيطرة أو مقارنة وجرعت 3 مللترات من المحلول الملحي الطبيعي لمدة شهر وجرعت المجموعة الثانية الخلاصة الكحولية لأوراق نبات الهندباء البرية بواقع 500 ملغم/كغم/باليوم لمدة شهر كامل . وضعت جميع ذكورالتجربة في الأسبوع الأخير منها مع إناث خصبة لغرض دراسة تأثير المعاملات المختلفة على الخصوبة والكفاءة التناسلية لذكور أرانب التجربة. توصلت الدراسة إلى أن الخلاصة الكحولية لأوراق نبات الهندباء البرية أدت إلى ارتفاع معنوي (p≤0.01 ) في مستوى أنزيم AST وانخفاضا معنوياًp≤0.05) ) في مستوى ALT في مصل الدم. أن التجريع الطويل الأمد لمستخلص الايثانول لأوراق نبات الهندباء البرية أدى إلى توقف خصوبة ذكور الأرانب (عدم حصول الحمل) وانخفاضا معنوياً (p≤0.05) في أوزان خصي ذكور الأرانب مصحوبآ بانخفاض معنوي في تركيز النطف في مجموعة الحيوانات المعاملة . أن التجريع الطويل الأمد لمستخلص الايثانول لأوراق نبات الهندباء البرية لذكور الأرانب أدى إلى حدوث تغيرات واضحة في التراكيب النسيجية لجميع الأعضاء المدروسة (النخامية- الدرقية- الخصى ( مقارنة بأعضاء مجموعة السيطرة

Keywords

Fertility --- AST --- Sperm count.


Article
In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients

Author: Saeeda A. M. Alanssari
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 4 Pages: 394-399
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients. Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes. Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied. Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte percentage was significantly higher (P


Article
DNA Content of Human Spermatozoa with Respect To Sperm Morphology

Author: Munaf Salih Daoud
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 2 Pages: 136-140
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The studies on the relationships of sperm morphology and DNA was backdated for nearly 25 years. DNA and morphology was studied in mammalian sperm by flow cytometry and DNA content of different sperm types for normal and abnormal subfertile humans was recorded. The effects of some factors like drugs, cigarette smoking, cryopreservation and sexual abstinence and others on sperm morphology and DNA damage, denaturation and fragmentation in both normal fertile and abnormal infertile men were reported. Several methods for the evaluation and estimation of DNA in spermatozoa have been reported.AIM OF THE STUDY:The objective of the present study was the determination of the DNA content of spermatozoa in subfertile persons and its correlation with sperm morphology.METHODS:A random sample of 66 subfertile males attending the Institute for Embryo Research and Infertility treatment, University of Baghdad,were enrolled in this study through years 2004-2005.The patients age ranged 20-45 years and their semen samples were assessed for sperm morphology by direct light microscopy. DNA content (μg/ml) was estimated using a microchemical spectrophotometric method, and data were classified into two groups according to sperm percent abnormality (below and above 50%).RESULTS:The results showed that no statistically significant difference (P > 0.05) of DNA content between the two groups was obtained. The DNA content (μg/ml) showed a negative non significant correlation with percent morphology in the normal and abnormal groups (P>0.05).CONCLUSION:The level of the DNA content in the spermatozoa was not related to its percentage of morphological abnormalities .


Article
Sperm DNA content: An overall correlation study with sperm count, motility and morphology

Author: Munaf S. Daoud*Ph.D د. مناف صالح داود
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 2 Pages: 277-280
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: To determine the DNA content in subfertile patients and correlate it with seminal sperm parameters, (count, motility and morphology).Design: Prospective observational study.Setting: College of Medicine, Department of physiological chemistry and Institute for Embryo research and Infertility treatment, University of Baghdad.Methods: A random sample of 58 subfertile male patients undergoing semen evaluation and their age ranged from 20-45 years were studied. Semen samples were assessed for seminal sperms (count , motility and morphology) by direct light microscopy. Sperm DNA content was estimated using a microchemical spectrophotometric method .The data were categorized into four groups according to normal and abnormal sperms count ( million / ml ),percent motility and percent morphology. The normal limits of those sperm function parameters were according to WHO criteria. The four groups were named as Normozoospermic (NZS) (control) , Normoasthenozoospermic (NAZS) ,Normoasthenoteratozoospermic (NATZS) and oligoasthenoteratozoospermic (OATZS) groups.Results: The Mean ± SD of the four groups were of significant difference (P> 0.05 ) with respect to sperm count, sperm percent motility and morphology with exceptions of certain groups. Moreover, a non significant difference (P< 0.05) was found with respect to DNA content (µg /ml) and (µg/sperm) except for Gr. IV which showed a significant difference when compared to others.The correlation coefficients (r values) between sperm count and sperms motility, morphology and DNA content (µg/ml) were non significant in the four groups. Noticeably, the DNA content (µg/sperm) was statistically of significant (P>0.05) negative correlation with sperm count in all groups.Conclusion: All groups were of significant difference (P<0.05) among their Mean ±SD values of their count, motility and morphology. There were few exceptions. No significant differences (P>0.05) were found with respect to DNA content (µg/ml or µg/sperm) except for oligoasthenoteratozoospermic group compared to others. No significant correlation was found between sperm count and each of sperms motility , morphology and DNA content (µg/ml) in the four groups .However, the DNA content (µg/sperm) had a statistically significant (P<0.05) negative correlation with sperm count in all groups.Keywords:DNA, sperm concentration(count million/ml ), Normozoospermic(NZS),Normoasthenoszoopermic(NAZS),Normoasthenoteratozoospermic(NATZS), Oligoasthenoteratozoospermic (OATZS ) .

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