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Evaluation of Antimicrobial Activity of Mineral Trioxide Aggregate (MTA)

Authors: Ennas Y Shehab --- Maha M Al–Bazzaz --- Mahmoud Y Taha
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2009 Volume: 9 Issue: 2 Pages: 216-219
Publisher: Mosul University جامعة الموصل


Aims: To evaluate the antimicrobial activity of MTA against selected microorganisms compared with a
widely used root end filling materials. Materials and methods: Fifteen mm discs of MTA, GIC and
Amalgam were prepared and three types of microorganisms; two bacteria and one fungus, were grown
in 4 ml of brain heart infusion broth for 18 hr. Then 0.5 ml of each growth was spread over selected
media (three plates for each sample) and the discs were applied on the agar, incubated for 24–48 hr and
the zone of inhibition was measured. Results: Amalgam did not demonstrate any antimicrobial activity,
whereas MTA showed antimicrobial effect against all tested microorganisms and was highly significant.
GIC showed antibacterial activity comparable to MTA but failed to produce antifungal effect.
Conclusions: MTA demonstrated antibacterial and antifungal effect, while GIC showed only antibacterial
activity, whereas Amalgam did not show any activity.


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