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A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice

Author: Shayma J. Ahmed* PhD
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 220-222
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications

Keywords

DNA --- Eukaryotes --- extraction --- Mice --- bone marrow --- Sperm --- Hind III digestion

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