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Article
Is Chlamydia Pneumonia An Independent Risk Factor In Ischemic Heart Disease

Authors: Eman Sh. AL-Obeidy --- Eman Sh. AL-Obeidy
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 2 Pages: 151-154
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Immune system may interplay between Chlamydia pneumoniae infection and ischemic heart disease (IHD).Major histocompatibility genes regulate innate and adaptive immunity..OBJECTIVE:This study was established to shed light on the possible association between ischemic heart disease (IHD) with Chlamydia pneumoniae infection and HLA antigens.PATIENTS AND METHODS:Microlymphocytotoxicity assay has been applied for HLA-typing of 150 blood samples of 100 IHD patients and 50 healthy normal controls, In addition enzyme linked immunosorbant assay (ELISA) used to detedct C pnuminiae IgA and IgG.RESULTS:An increased frequency of HLA-A*2, B*35 alleland HLA-A*2-B*35 haplotype was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively.CONCLUSION:This finding demonstrated that the HLA-B*35 positive haplotypes confer the C.pneumoniae-related risk for IHD. HLA-DR3,DR4 and DR7 might play a role in AIH susceptibility


Article
Association of Fibrinogen as a Myocardial Infraction Risk Factor in Men

Authors: Basil. N. Saeed --- Eman Sh. AL-Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 4 Pages: 392-394
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Acute myocardial infarction (AMI) is an irreversible myocardial injury and necrosis caused by serious and long term ischemia. Fibrinogen is considered as
one of the probable risk factors of myocardial infarction.
Patients and methods: 1.8-mL venous blood specimen from 40 patients with MI and 50 control subjects was obtained and put it into tubes containing 0.2 mol/L trisodium citrate. Plasma fibrinogen level was determined by the method of Clauss (Diagnostic Stago, France). Results The mean age of patients was (45.2 ± 6) years in patients with premature myocardial infarction and (45.06±5) years in the control group (p =0.07). There were no statistically significant relationships between the two groups in history of premature myocardial infarction in their first-degree relatives (p =0.05), cigarette smoking (p =0.06), diabetes (p =0.08), or hypertension (p =0.071). The mean plasma fibrinogen in patients (495 ±10.2mg/dL) was elevated markedly compared with the control group (465±8.4 mg/dl) ( p =0.01) . Hyperfibrinogenemia (>450 mg/dL) was detected in 87.5% of patients and 52.0% of controls. Conclusion: This study introduced fibrinogen as a risk factor for premature coronary artery disease in Iraqi men


Article
HLA prevalence in Iraqi patients with ischemic heart disease

Authors: Eman Sh. AL-Obeidy --- Basil N. AL-Dileamy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 2 Pages: 182-184
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The etiology of ischemic heart disease (IHD) is believed to have an immunological component. Association with human leukocyte antigens (HLAs) has been previously reported, particularly with DR6.Patients and methods: 75 cardiac patients were admitted to the coronary care unit, Baghdad Teaching Hospital over the period October 2008-May 2009 with the clinical diagnosis of acute coronary syndrome and STEMI myocardial infarction their ages range was (25-82) years the number of male was (55) (73.3%) and female was (20) (26.7%). All cases have routine ECG, cardiac marker’s measurements, routine haematological, Biochemical test and 2mls of blood reserved for HLA study.
Results: It was found that HLA-DR1 (8%) (P 0.001) has significant risk factor in the development of ischemic heart disease while HLA B (62%) (P 0.0009) has a protection factor in ischemic heart disease.
Conclusions: Our result suggests that strong relation between the incidence of acute ischemic episode (acute coronary syndrome and STEMS myocardial infarction and HLA-DR1) which mean that there may be predisposing genetic factor for the development ischemic heart disease.


Article
Possible Association of HLA-DR and DQ Molecules with Autoimmune Hepatitis in Iraqi Patients

Authors: Raghad J H AL-Akayshi --- MBChB, PhD --- Eman Sh. AL-Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 1 Pages: 49-52
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region seem to influence susceptibility to certain diseases.
Patients and methods: Polymerase chain reaction-Sequence Specific Primers PCR-SSP is the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls.
Results: An increased frequency of HLA-DR3, DR4 and DR7 was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively, while DR*0211 (DR2) may be formed the basis for protection against the disease. HLA-DQ on the other hand, yielded on association in Iraqi patients with AIH.
Conclusions: This finding demonstrated that HLA-DR3, DR4 and DR7 might play a role in AIH susceptibility.
Key words: autoimmune hepatitis, Human leukocyte antigen (HLA), PCR –SSP assay.

References:

Keywords


Article
Production of Different Cytokines in Acute and Chronic Hepatitis C Virus.
انتاج مختلف السايتوكينات في التهاب الكبد الفايروسي ج الحاد والمزمن

Authors: Khalid A. Al-Khazraji د.خالد الخزرجي --- Eman Sh. Al- Obeidy د.ايمان العبيدي
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2010 Volume: 23 Issue: 2 Pages: 134-140
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Summary:Background: the role of pro-inflammatory cytokines has been documented in acute phase reactions in the liver, in normal proliferation of hepatocytes, in autoactivation of Kupffer cells and proliferation of immunocyte, in cirrhotic processes in the liver and in regeneration of the organ in chronic hepatitis C.Objective: assess whether there is a differential production of Th1 or Th2 associated cytokines in the course of acute and chronic HCV infection.Patients & methods: Serum level of IL2, INF gamma (Th1 associated cytokines) and IL10 (Th2 associated cytokines) were measured in 12 patients with acute HCV, 50 patients with chronic HCV infection and 50 healthy volunteers. Results: mean serum level of IL-2 was significantly higher in chronic hepatitis C (30.5 ± 16.02) than in acute cases (26.9 ± 13.01) and controls (11.3 ± 2.03). Acute HCV cases had a higher mean IL-10 level (99.3 ± 17.0) than controls (5.0 ± 2.0) and chronic HCV cases had a higher mean IL-10 level (100.9 ± 53.7) than acute HCV cases. Mean serum level of INF gamma where nearly equal in all three groups. Conclusion: this study suggests weak response of Th1 cells in acute HCV infection and a possible suppressive Th2 cell response in chronic HCV, which might explain the failure of viral clearance and a weak cellular immune response.Key wards: Chronic hepatitis-C; Pro-inflammatory cytokines; IL-2; IL-10 NF-gamma.

الملخص:الخلفية:تم اثبات دور السايتوكينات المؤيدة للالتهاب في تفاعلات المرحلة الحادة في الكبد, في التكاثر الطبيعي لخلايا الكبد,في التنشيط الذاتي لخلايا كفر,في تكاثر الخلايا المناعية,في عمليات التشمع في الكبد,وفي تجدد العضو في التهاب الكبد الفايروسي ج المزمن. الاهداف:تقييم فيما اذا كان هناك انتاج نوع مختلف من السايتوكينات المرتبطة بخلاياTالمساعدة نوع1و2(Th1andTh2)خلال مرحلة العدوى بالتهاب الكبد الفايروسي ج الحاد والمزمن.المرضى وطرق العمل:تم قياس مستوى المصل من انترلوكين2 و انترفيرون كاما(سايتوكينات مرتبطة بخلاياTالمساعدة نوع1Th2)في 12 مريضا مصابا بالتهاب الكبد الفايروسي ج الحاد و50 مريضا مصابا بالتهاب الكبد الفايروسي ج المزمن و50شخصا اصحاء متطوعين.٭٭ معدل مستوى المصل من انترلوكين2كان اعلى بصورة ملفتة للنظر في التهاب الكبد الفايروسي ج المزمن(16,02±30,5)من التهاب الكبد الفايروسي ج الحاد(26,9±13,01)والكونترول(11,3±2,03) وسجلت حالات التهاب الكبد الفايروسي ج الحاد مستويات اعلى من انترلوكين10(99و3±17)من الكونترول(5±2).اما حالات التهاب الكبد الفايروسي ج المزمن فسجلت مستويات اعلى من انترلوكين10(100,9±53,7)من التهاب الكبد الفايروسي ج الحاد.٭٭معدل مستوى المصل من انترفيرون كاما كان تقريبا متساويا في الحالات الثلاث.الاستنتاجات:هذه الدراسة تبين استجابة ضعيفة للخلايا المساعدة1Th1في الحالات الحادة واستجابة مثبطة محتملة للخلايا المساعدة2Th2في الحالات المزمنة والذي من الممكن ان يفسر فشل تنقية الفايروس وضعف الاستجابة المناعية الخلويةالكلمات المفاتيح: التهاب الكبد الفايروسي ج المزمن,السايتوكينات المؤيدة لللالتهاب,انترلوكين1,انترلوكين10,انترفيرون كاما


Article
Hepatitis G virus infection among Iraqi patients with Chronic liver diseases

Authors: Faiza A.Mukhlis فايزة احمد --- Shatha F. Abdullah شذى فاروق --- Eman Sh.Al-Obeidy ايمان العبيدي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 3 Pages: 299-303
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The hepatitis G virus( HGV), also called hepatitis GB virus, as a member of the Flaviviridae family distantly related to hepatitis C virus (HCV), Little is known about the frequency of HGV infection, the nature of the illness, or how to prevent it. What is known is that transfused blood containing HGV has caused some cases of hepatitis. They infect humans, but are not known to cause human disease. This virus can be transmitted efficiently by blood transfusion and by other parenteral mechanisms. Transient and long lasting infections with HGV have been documented in man.Patients and methods: HBs Ag, Anti-HCV IgG and Anti-HGV IgG were detected by Enzyme-Linked Immunosorbent Assay (ELISA).HCV RNA on the other hand, has been detected using PCR technique in the serum of 75 Iraqi patients with chronic liver diseases in comparison to 15 healthy individuals.Results: HGV infection was detected in 25% of blood donors, 30% of chronic hepatitis C, 25% of chronic hepatitis B, and 20% of cryptogenic chronic liver disease. HGV infected patients tended to be younger than non-infected patients but no differences concerning sex, possible source of infection, clinical manifestations, biochemical and virological parameters, or severity of liver lesions were found.Conclusions: The percentage of HGV infection in chronic liver disease seems to be relatively high in our area 19 out of 90cases (21.11%). Infection with HGV does not seem to play a significant pathogenic role in patients with chronic liver disease related to chronic HBV or HCV infection, or in those with cryptogenic chronic liver disease. Key words: HGV, chronic liver disease, blood donors.


Article
POSSIBLE ROLE OF IL-1-α AND TNF-α IN BREAST CANCER
الدور الاحتمالي للمدورات الخلوية البين بيضاضي 1 –الفا وعامل تنخر الورم-الفا في سرطان الثدي

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Abstract

Background: Cytokines have been used as biomarkers in research for prognosis and have been associated with symptoms and adverse outcomes in multiple conditions, including breast cancer.Objectives: To estimate the concentration of IL-1-α and TNF-α in serum of breast cancer (BC) patients compared with control groups and to detect if there is association of serum levels of these interleukins with disease development. Subjects and Methods: The levels of IL-1-α and TNF-α were measured by ELISA method in sera of 45 BC patients, 12 patients with benign breast lesions and 23 apparently healthy controls.Results: Present study was demonstrated that IL-1-α and TNF-α levels were significantly elevated in serum of BC patients as compared with controls (p<0.001), this elevation were significantly associated with poor prognostic factors including advanced stage and estrogen and progesterone receptors-negative status.Conclusions: Evaluation the serum level of IL--α1and TNF-α may be helpful as predictive non-invasive tests for tumor development in breast cancer patients.Keywords: Breast cancer, IL--α1, TNF-α.

خلفية الدراسة: تستخدم المدورات الخلوية كعلامات بايولوجية في البحث لبيان مال المرض وارتبطت بالاعراض والنتائج المضادة في حالات عديدة من ضمنها سرطان الثدي. تستخدم المدورات الخلوية كعلامات بايولوجية في البحوث لبيان مأل المرض حيث وجد بان لها علاقة بالاعراض السلبية في العديد من الحالات المرضية من ضمنها سرطان الثدي. هدف الدراسة: لتقييم تركيزالبين بيضاضي1- الفا وعامل تنخر الورم-الفا في مصل مرضى سرطان الثدي مقارنة بمجموعتي السيطرة ولتحديد فيما اذا كان هناك مصاحبة بين المستويات المصلية لهذه المدورات الخلوية مع تقدم المرض.الاشخاص وطرائق العمل: قيست المستويات المصلية للبين بيضاضي1- الفا وعامل تنخر الورم-الفا بتقنية فحص مترابطة الخميرة بمادة ماصة المناعة (ELISA) لـ 45 مريضة مصابة بسرطان الثدي, 12 مريضة مصابة بامراض الثدي الحميدة و 23 امراة سليمة ظاهريا كمجموعة ضابطه. النتائج:أظهرت الدراسة الحالية ارتفاع احصائي مهم للمستويات المصلية للـ البين بيضاضي1- الفا وعامل تنخر الورم-الفا في مرضى سرطان الثدي مقارنة بمجموعتي السيطرة (p<0.001) , هذا الارتفاع كان مصاحبا وبشكل مهم احصائيا للمراحل المتقدمة للمرض والحالة السالبة لمستقبلات الاستروجين والبروجيستيرون. الاستنتاجات: تقييم المستوى المصلي للبين بيضاضي1- الفا وعامل تنخر الورم-الفا قد يكون مساعد كفحوصات غير متدخلة تنبؤية لتطور الورم في مرضى سرطان الثدي. مفتاح الكلمات:سرطان الثدي, البين بيضاضي1- الفا, عامل تنخر الورم-الفا.

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