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An Evaluation of the Antibacterial Effects of Rue and Sage Extracts

Authors: Siba M Al–Haliem --- Maha M Al–Bazzaz --- Ghada Y Abdul– Rahman
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 1 Pages: 55-61
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To investigate the antimicrobial effect of different concentrations of ethanolic and aqueous extracts of Sage and Rue (0.5%, 0.05%, 0.005%) on bacterial samples. They were iso-lated from root canal system in vitro, and the results were compared with that of chlorhexidine glu-conate (0.2%) as a positive control and normal saline as a negative control. Materials and Methods: Eighteen hours cultured bacterial samples of the root canal were used. To each 4ml of Brain Heart Infusion Broth (BHI), 0.1 ml of both bacterial sample and the examined solution Sage, Rue and chlorhexidine were added. Cultures were incubated at 37°C for 48 hours. The absorbance val-ues of the cultures were determined by the turbidity method using spectrophotometer at (550 nm). Re-sults: The results showed that both ethanolic and aqueous extracts of Sage and Rue have anti-bacterial effects in all concentrations with a significant variance compared with chlorhexidine against root canal microorganisms, while normal saline failed to show any antimicrobial activity. The results also showed that the ethanolic extracts of both plants have a higher antibacterial activity than the aqueous extracts. Conclusions: Rue and Sage demonstrated antibacterial effects on the root canal microorganisms (both aerobic and anaerobic) when compared with chlorhexidine.

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Article
Disinfection of Extracted Teeth for Den-tal Researches

Authors: Arjwan M Shukur --- Wiaam MO Al-Ashou --- Ghada Y Abdul-Rahman
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 1 Pages: 158-161
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To determine the effectiveness of different disinfection methods on extracted human teeth using five types of bacteria Proteus species, Escherichia coli, Kelebsiella species, Staphylococcus aureus, Streptococcus mutans. Materials and Methods: In this study extracted non-carious teeth were divided into five groups according to the type of bacteria that were inoculated inside the pulp chambers. Each group of the teeth were subdivided into six groups; group A: teeth were immersed in 2.5% NaOCl for 1week, group B: teeth were immersed in 1% NaOCl for 1week. Group C: teeth were autoclaved at 121C for at 15 Ibs psi for 15 minutes, group D: teeth disinfected using microwave for 3 minutes, group E: teeth disinfected using microwave for 6 minutes, group F: control group in which the teeth im-mersed in normal saline for seven days at room temperature. Each tooth aseptically placed in individual test tube with growth media. Simples were examined after 24h. Results: showed that autoclave, mi-crowave (when used at sex and three minutes), sodium hypochlorite at 2.5% prevented the growth completely in all types of the bacteria that were used to infect the teeth involved in this study.

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