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Article
Occurrence of Helicobacter pylori Specific antibodies in patients with Iron-deficiency anemia
التحري عن اضداد النوعية لبكتريا Helicobacter pylori عند مرضى فقر الدم نوع نقص الحديد

Author: Ishraq Ahmed Chiad
Journal: AL-TAQANI مجلة التقني ISSN: 1818653X Year: 2010 Volume: 23 Issue: 5 Pages: 41-45
Publisher: Foundation of technical education هيئة التعليم التقني

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Abstract

This study evaluates the prevalence and significance of Helicobacter pylori (H. pylori) infection in patients with iron- deficiency anemia. Analysis serum samples for the presence of H. pylori antibodies by ELISA was performed in 123 iron deficiency anemia sample and 100 sample for blood doners as control. Highly percentage of H. pylori antibodies positive was found in gastric patients with iron- deficiency and it was higher in females (66%) than males (55%) respectively. Majority of cases (more than 79%) from rural area. Percentages of patients with history of anemia and positive H. pylori is (55.2%), while (84%) for history of gastritis and positive H. pylori in blood doners (P < 0.001). From this study concluded that the sero-prevalence of H. pylori in patients and doners are (59%), (22%) respectively.

قيمت الدراسة مدى شيوع واهمية الأصابة بالـ H. pylori لدى مرضى فقر الدم نوع نقص الحديد. حيث حددت الأجسام المناعية المضادة للبكتريا بواسطة اختبار مقاييسة الروز المناعي المرتبط بالأنظيم. شملت الدراسة لدى 123 عينة لمرضى نقص الحديد و 100 عينة اخرى لمتبرعي الدم كسيطرة. وجد ان اعلى نسبة اصابة بالـ H. pylori عند المرضى المصابين بالتهاب المعدة مع وجود فقر دم نوع نقص الحديد. ان نسبة الاصابة عند الأناث اعلى من الذكور 66%, 55% على التوالي. كما عرف ان غالبية الحالات (اكثر من 79%) من المناطق الحضرية. نسبة الأصابة بالـ H. pylori لدى مرضى لهم تاريخ سابق بفقر الدم كانت 55.2% بينما 84% لمتبرعي الدم ولهم تاريخ بالتهاب المعدة, وكانت هذه النتائج ذات تأثير معنوي مهم (P <= 0.001). نستنتج من هذه الدراسة ان نسبة شيوع H. pylori عند مرضى نقص الحديد اعلى من متبرعي الدم وبنسبة تتراوح 59%, 22% على التوالي.

Keywords

H. pylori --- Iron-deficiency --- Anemia


Article
Peptic Ulcer in a Group of Iraqi Diabetic Patients
القرح الهضميه لدى مجموعه من المرضى العراقيين المصابين بداء السكري

Authors: Sarab K. AL-Rawy د.سراب خاشع الراوي --- Sabeha Al-Bayati د.صبيحه البياتي
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2010 Volume: 23 Issue: 3 Pages: 190-195
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Both acute and chronic hyperglycemia can lead to specific gastrointestinal (GI) complication.H.pylori infection is well established cause of dyspepsia, although all the three factors; lifestyle, digestive fluids, and H.pylori infections may play a role in ulcer development.Objective:-This study attempts to determine the role of some factors like lifestyle, duration of dyspeptic symptoms, state of diabetic control and H.pylori infection in diabetic patients.Methods:-The study was carried in the endoscopy unit of Al-Yarmook Teaching hospital. The total blood samples of 178 subjects with 80 antral biopsies obtained from four groups; 30 diabetic with peptic ulcer (P.U) as patients group and control groups involving 54 non diabetic with no peptic ulcer, 50 non diabetic with peptic ulcer and 44 diabetic with no peptic ulcer. All participants answered a special questionnaire, and every gastric biopsy specimen was subjected to cultural, histology, rapid urease test for H.pylori diagnosis in addition to blood samples for enzyme- linked immunesorbent assay ( ELISA) as a serologic study and to estimate plasma glucose level.Results: Some dietary factors such as spicy foods 60% and smoking 54% had more influence on the incidence of H.pylori infection with peptic ulcer disease (PUD). Some dyspeptic symptoms (nausea/ vomiting 93.3%, loss of weight 80%, hematemesis /N-melaena 67%) are seen more in diabetic with P.U.D(patients group) when compared with the other control groups. ELISA test for diagnosis of H.pylori infection revealed that there is no significant difference (P>0.05) to the incidence of H.pylori infection in diabetes either with PU 40% or with no PU 59%. The H.pylori infection was positive in diabetic with P.U (patients group) 73.3% who have dyspeptic symptoms <1year, when compared with (control group) non diabetic with P.U 34% (P<0.01).Conclusion: Some dietary factors and smoking increased the incidence of P.U.D. Poor glycemic control was not associated with significant increase in the incidence of P.U.D. Duration of dyspeptic symptoms < 1 years with positive result for H.pylori infection was higher in diabetic patients with P.U.DKeyword: H.pylori, peptic ulcer, diabetes mellitus.

الخلفيه:يؤدي ارتفاع نسبه السكر بالدم سواء الحاد منه او المزمن الى حصول اختلاطات معدّيه معويه ،حيث ثبت ان الاصابه بجرثومه الملويه البوابيه يسبب عسر الهضم ،على الرغم من ان العوامل الثلاثه وهي :طريقه المعيشه ،السوائل الهاضمه والاصابه بجرثومه الملويه البوابيه بمجموعها تلعب دورافي تطور القرح الهضميه.الهدف:تهدف الدراسه لتحديد دور عده عوامل منها :طريقه المعيشه ،ارتفاع نسبه السكر بالدم، المده الزمنيه اللازمه لظهوراعراض عسر الهضم وغيرها والتي تعد كعوامل مؤثره في امكانيه حدوث امراض القرح الهضميه وبالتالي تشخيص الاصابه بجرثومه الملويه البوابيه لدى المرضى المصابين بداء السكري.طريقه البحث:اجريت الدراسه بوحده الناظور/مستشفى اليرموك التعليمي،و تضمنت الدراسه 178 عينه دم و80 خزعه نسيجيه والتي اخذت من الغار المعدّي ،حيث شملت الدراسه 30 من المرضى المصابين بداء السكري والقرح الهضميه ومجاميع السيطره المتضمنه 54 غير مصابين بداء السكري ولا القرح الهضميه و50 مصابين بالقرح الهضميه فقط و44 مصابين بداء السكري فقط. لقد اجاب المشاركين علىالاسئله الخاصه بنموذج الاستطلاع ،كما واخضعت عينات الخزع النسيجيه التي اخذت من الغار المعدّي لكل مريض يعاني من القرح الهضميه سواء أكان مصابا او غير مصابا بداء السكري الى الزرع البكتريولوجي ،الفحص النسيجي وتحليل اليوريز السريع لتشخيص جرثومه الملويه البوابيه كما وشخصت باستخدام طريقه مقايسه الممتز المناعي المرتبط بالانزيم والتي اجريت على مصول كل من مجاميع المرضى والسيطره والذين تم فحص مستوى السكر بالدم لديهم. النتائج:اوضحت النتائج ان هناك بعض العوامل المرتبطه بعادات الغذاء كالتوابل وبنسبه60% والتدخين وبنسبه54% تكون ذات تاثير اكبر على المرضى المصابين بالقرح الهضميه. لوحظ ان بعض اعراض عسر الهضم ( كالغثيان والتقيء 93.3 % ، وفقدان الوزن 80% ،وقيئ الدم 67%) كانت بنسبه اعلى عند المرضى المصابين بداء السكري والقرح الهضميه (مجموعه المرضى) مقارنه بمجاميع السيطره،وان 59% من مرضى السكري والمصابين بالقرح الهضميه( مجموعه المرضى) و40% من المصابين بالسكري فقط( مجموعه السيطره)قد اعطوا نتائج ايجابيه لجرثومه الملويه البوابيه باستخدام طريقه مقايسه الممتز المناعي المرتبط بالانزيم والتي اجريت على مصولهم حيث لم تكن النتائج ذات تاثير مقبول معنويا،كما واعطت التنائج الخاصه بتشخيص جرثومه الملويه البوابيه نسبه 73.3% لدى المرضى المصابين بداء السكري والقرح الهضميه( مجموعه المرضى) واللذين يعانون من ظهور اعراض عسرالهضم(<1سنه) مقارنه بالمرضى المصابين بالقرح الهضميه فقط (مجموعه السيطره)34% حيث كانت النتائج مقبوله احصائياP<0.05.الاستنتاجات: ان لبعض العادات الغذائيه والتدخين تاثير في زياده نسبه الاصابه بالقرح الهضميه لدى المرضى المصابين بداء السكري كما وان عدم او ضعف السيطره على نسبه السكر بالدم لا يعد من العوامل التي تزيد من نسبه الاصابه بالقرح الهضميه وان وجود اعراض عسر الهضم لمده تقل عن السنه واللذين اعطوا نتائج ايجابيه لجرثومه الملويه البوابيه كانت بنسبه اعلى لدى المرضى المصابين بداء السكري والقرح الهضميه.


Article
Molecular and Immunopathological Role of Nuclear Factor K B Detected By Insitu Hybridization in Pathogenesis of Chronic Atrophic Gastritis in Iraqi Patients

Author: Ali Ibrahim Ali
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 187-214
Publisher: Diyala University جامعة ديالى

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Abstract

Chronic Atrophic gastritis (CAG) is a histopathological entity characterized by chronic inflammation of the gastric mucosa with loss of gastric glandular cells and replacement by intestinal-type epithelium and fibrous tissue. Atrophy of the gastric mucosa is the endpoint of chronic processes, such as chronic gastritis associated with Helicobacter pylori infection. This study designed to determine the molecular role of NFkB signaling on Fas receptor mediated apoptosis in H.pylori associated CAG cases. Forty five patients suffering from CAG that proved via endoscopical and histopathological examination were eligible for this study. Males represent (73.33%) and the rest (26.67%) were females with a mean age of 39.26 years .twenty apparently healthy volunteers (10 male and 10 female) with the mean age 38 years and age range (23-58) years were enrolled as control group. Multiple mucosal biopsy specimens were taken from the inflamed area of gastric mucosa for rapid urease test other biopsy specimens were fixed with 10% buffered formalin for preparation of paraffin embedded tissue blocks to prepare slides for histological examination using haematoxylin-eosin stain to evaluate the grades of Polymorphonuclear leukocytes(PMNs) and lymphocytes infiltration . Insitu hybridization technique (ISH) using biotinylated long DNA probe for human NF-KB Gene and for human FAS gene as well as H.pylori Cag A gene were used for detection of NF-KB and Fas mRNA and Cag A mRNA expression in tissue infiltrated lymphocytes and neutrophils as well as gastric epithelial cells and for detection of H.pylori Cag A. This study explained that there was a statistical significant positive linear relationship between lymphocytes grade and Fas mRNA expression in PMNs and gastric epithelial cells. PMN grade has significant positive linear relationship with each of the following markers: Tissue Fas mRNA expression. Tissue NFkB mRNA expression, lymphocytes NFkB mRNA expression, PMN NFkB mRNA expression, Cag A mRNA expression. Lymphocytes Fas mRNA expression has significant positive linear relationship with PMN Fas mRNA expression and Cag A mRNA expression .PMN Fas mRNA expression has significant positive linear relationship with Cag A mRNA expression and negative linear relationship with tissue NFkB mRNA expression . Tissue Fas mRNA expression has significant positive linear relationship with tissue NFkB mRNA expression ; lymphocytes NFkB mRNA expression ; PMN NFkB mRNA expression ;Cag A mRNA expression . Tissue NFKB mRNA expression has significant positive linear relationship with lymphocytes NFkB mRNA expression; PMN NFkB mRNA expression. Lymphocytes NFkB mRNA expression has significant positive linear relationship with PMN NFkB mRNA expression. This study conclude that NFkB play a vital role in determination of immunopathological and inflammatory events of gastritis that leads finally to development of atrophic changes


Article
Helicobacter Pylori Infection in Patients with Hematological Diseases

Author: Najlaa Badir Al-Awadi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 1 Pages: 74-79
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori (H. pylori) is a well known bacterial pathogen implicated in gastric diseases. Some studies refer to it's possible role in some hematological diseases.OBJECTIVE:The aim of this study is to determine the association between Helicobacter pylori infection and some hematological diseases.MATERIALS AND METHODS:This is a case-control study of 337 patients with a hematological disease (including acute and chronic leukaemias, Hodgkin's and non Hodgkin's lymphomas, hairy cell leukaemia, multiple myeloma, immune thrombocytopenic purpura and aplastic anemia) who were admitted to or attended hematology center at Marjan Teaching Hospital – Babylon – Iraq during the period from 1/7/2006 to 1/1/2009.Age and sex matched control group of 337 patients who have no hematological disease. They were taken during the same period and from the same geographical area (Babylon governorate).Tests for H. pylori were done for both groups using One Step H. pylori Test Device with 93.7% total accuracy rate.RESULTS:H. pylori test was positive in 33.5% (113/337) and 37.3% (126/337) of control and patient groups respectively, while dyspeptic symptoms were found in 18.1% (61/337) and 39.4% (133/337) of control and patient groups respectively.CONCLUSION:There was no significant association between H. pylori infection and hematological diseases tested in this study in Iraqi patients from Babylon, however dyspeptic symptoms were more common in patients on steroids or chemotherapy


Article
Risk factors for perforated duodenal ulcer in sulaemania city
عوامل خطر الإصابة بقرحة الاثني عشر المثقبة في مدينة السليمانية

Authors: Muhammed Abulrahman Mahmood --- Zanko Sherko Fathalah --- Taher Abdullah Hawrame
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2010 Volume: 14 Issue: 3 Pages: 61-66
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and Objective: This prospective study was to evaluate the probable risk factors for perforation of duodenal ulcer, highlighting any prevalent one in the occurrence of perforation in Sulaemania city. The study was designed to be performed on patients with perforated duodenal ulcer attending the emergency department in Sulaemania teaching hospital over the period of this study.Method: This study was conducted between 1st of March 2009 till the 1st of October 2009 involving 50 patients with perforated duodenal ulcer attended the emergency department in Sulaemania teaching hospital. A number of probable risk factors for the patients were studied.Results: Out of 50 patients, 41(82%) were males, 9(18%) were females and the male to female ratio was (4.5:1), about 32% of the patients were more than 60 years old. Patients described themselves as having a nervous personality were 31(62%) and the number of patients lived in city center were 28(56%). Smoker patients were 36(72%) and 33 patients (66%) had a history of NSAIDs ingestion.Conclusion: Smoking, NSAIDs ingestion, sex, age and stress played a major role as risk factors in the occurrence in duodenal ulcer perforation. There is a seasonal variation in incidence of perforation including high incidence during spring and autumn.

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