research centers


Search results: Found 6

Listing 1 - 6 of 6
Sort by

Article
Helicobacter Pylori Infection in Diabetic Patients

Author: Sabeha M. Al-Bayati
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2010 Volume: 9 Issue: 1 Pages: 31-34
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background H Pylori infection could be regarded as the commonest infection worldwide, it had been suggested that H pylori infection is more frequent among diabetics. Objective This study had been designed to define any significant association between diabetes and H pylori infection, to evaluate different demographic features of patients with diabetes mellitus who have H pylori infection, assess any relation between H pylori infection and the metabolic control of diabetes mellitus and to estimate the frequency of different oesophagogastroduodenoscopy findings among diabetic population with H pylori infection. Methods This study enrolled 50 patients with diabetes mellitus and another 50 non-diabetic patients (as a control group). The patients attended Gastrointestinal unit at Al-Yarmouk Teaching Hospital Baghdad/Iraq during the period between the 1st of March2004, to the 31st of November 2004. Full history was taken and clinical examination, investigation to assess the glycemic control and esophagogastroduodenoscopy (OGD)had been done to all of them. H pylori status had been detected by positive Rapid Urease test and histopathology and/or ELIZA test for anti-H pylori IgG. Results This study revealed that 26diabetic patients (52%) were H pylori positive, while 14 non-diabetic patients (28%) were H pylori positive which is statistically significant (X2=6.0,P=0.01). patients aging 60 year old or more (24 patients, 48% of the sample) 18 of them had positive H pylori status, 20 patients out of the 26patients with positive H pylori status (76.9%) had poor glycemic control, 84.6% of those with positive H pylori status (22 out of 26 patients) had glycated hemoglobin level of 8% and greater, 61.8% of those with positive H pylori status (16 patients) were diabetic for more than 10 years. 61.5% of those with positive H pylori status had one or more of the chronic diabetic complications, 12 patients had OGD finding consistent with duodenitis, all of them were H pylori positive, OGD examination revealed 20 cases without active disease, 18 of them had negative H pylori status. Conclusions This study indicates that Helicobacter pylori infection is more common in diabetic patients. Frequency of H pylori infection is higher among elderly diabetics, those with long standing, poorly controlled and with diabetic complications.


Article
The Detection of Neutrophiles in Gastric Mucosa of Patients Suspected to be Infected with Helicobacter Pylori Using Leukostix Nassir Enssief Mohsun*,

Authors: Suhaila Saadallah *** --- Raji H AL-Hadithi**,
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 4 Pages: 480-483
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori has been detected in many populations and associated with inflammation ofgastro duodenal mucosa. Colonization of the stomach by Helicobacter pylori occurs in more thanhalf of human population worldwide. It is the principle cause of chronic active gastritis, peptic ulcerand gastric cancer.OBJCTIVE:To detect neutrophiles in homogenates biopsied gastric mucosa semiquantitatively using rapidleukocyte strip test (leukostix).METHODS:A total of 115 patients (74 males, 41 females) referred to The Gastrointestinal Tract Center andGastroscopy Department of Baghdad Medical City and subjected to gastroscopy were included in thisstudy during the period from November 2004 to May 2005.RESULTS:The sensitivity and specificity of leukostix at the initial examination were 95.8%, 88.23%respectively.CONCLUSION:The leukostix test, using biopsied samples of gastric mucosa was excellent for quantitativedetermination of neutrophils in patients infected with H pylori.


Article
IN SILICO STUDY FOR PREDICTION OF DRUGTARGETS IN HELICOBACTER PYLORI
Helicobacter pylori دراسة حاسوب للتنبؤ بالأهداف الدوائية للبكتريا

Author: زينب هاتف عباس الركابي
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 4 Pages: 753-769
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

.ABSTRACT
In Silico Study, was conducted to search and predict essential biotargets which
might be as drug targets in Helicobacter pylori. Gene sequences (Protein sequences)
of the bacterium and its host (Homo sapiens) were retrieved from the Database for
Essential Genes (DEG) (http://tubic.tju.edu.cn/deg/).
The retrieved sequences were subjected to alignment process using BLASTP
2.2.23+(http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG
_DEF=blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seq ) with
different bioinformatic parameters and different matrices such as
PAM70,BLOSUM62 at different levels of E-values "0.001, 0.01, 0.05" to estimate
the essential bacterial genes that non-homologous with the human. Results showed
that 55 genes out of 323 essential bacterial genes have no homology with human
essential genes (118). The products of these genes (55 genes) were subjected to
programs to estimate the cellular location such as PSORTb v.3.0
(http://www.psort.org/psortb/index.html) and TMHMM
(http://www.cbs.dtu.dk/services/TMHMM/). The two programs showed that 22%
of non-homologous genes (12 protein) were associated with cell membrane, 24%
(13 protein) had unknown location, and the rest 54% (30 protein) were found in
the cytoplasm. It could be predicted that the membrane proteins might be good
targets for vaccine development and as well for antibiotics affected the cell
membrane.

الخلاصةللبحث عن الأهداف الحيوية التي يمكن أن تصلح أن In Silico تم إجراء دراسة على الحاسوبتم جمع تواليات الجينات الأساسية .Helicobacter pylori تكون أهدافاً دوائية في بكتريامن قاعدة المعلومات (Homo sapiens) (البروتينات الأساسية) للبكتريا ومضيفها الإنسانأُخضعت نواتج الجينات الأساسية .(http://tubic.tju.edu.cn/deg) للجينات الأساسيةBLASTP للبكتريا والإنسان لعمليات الإصطفاف بإستعمال+ 2.2.23http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=لتحديد . blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seqالبروتينات الأساسية غير المتشابهة للبكتريا مع بروتينات المضيف لتمثل أهدافاً دوائية وبإستعمال مؤشراتE-value وبقيم توقع مختلفة المستويات "PAM70,BLOSUM مختلفة من إذ المصفوفات " 620.05,0.01,0.001 ". أسفرت مجمل النتائج على إن هناك 55 جين من جينات البكتريا الأساسية " 323 " غير "متشابهة مع جينات الإنسان الأساسية " 118 ". أُخضعت البروتينات غير المتشابه لبرامج تحديد الموقع مثلTMHMM و (http://www.psort.org/psortb/index.html) PSORTb v.3.012 بروتين) هي بروتينات ) % وكانت النتائج 22 ،(/http://www.cbs.dtu.dk/services/TMHMM)30 بروتين) فكانت من ) % 13 بروتين) بروتينات غير محددة الموقع، أما الباقي 54 ) % غشائية و 24البروتينات السايتوبلازمية. يتوقع أن تكون البروتينات الغشائية أهدافاً جيدة سواء لإنتاج اللقاحات الوقائية أوالمضادات الحيوية التي تعمل على الأغشية. تهدف الدراسة محاولة إيجاد أهدافاً جديدة للأدوية نظراً لكونالبكتريا أصبحت مقاومة للمضادات والأدوية المستعملة في الوقت الحاضر


Article
Study the Prevalence of Helicobacter pylori Infection by Different Diagnostic Methods

Authors: Aseel I. Ibrahim --- Ibtihal N. Saeed ابتهال نوري سعيد
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2010 Volume: 19 Issue: 1 Pages: 74-81
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

A total of 41 patients with gastro duodenal symptoms (show signs of inflammation with or without duodenal ulcer) . 21 males (51.2%) and 20 female (48.8%) with an average age 0f (20 – 80) years old under going gastrointestinal endoscopy at Baghdad teaching hospital in internal disease clinical laboratory , between (February – June) 2009 . Biopsies specimen of antrum , gastric fundus ,& duodenal bulb were examined by the following methods (rapid urease test , Giemsa stain section to detect bacteria , & Haematoxilin and Eosin stained section for pathological study which are considered the gold standard methods , sera or plasma from these patients were tested by immunochromotography (ICM),serological method for IgG antibodies to H. pylori. History picture are( use of certain medication , tobacco , alcohol, and current infection are taken). The results showed that the percentage of prevalence (positive results)were (83%) by histopathological method while it gave only(73%) by serological method and(66%) by rapid urease test, and the prevalence in males was more than in females (44%), (39%)respectively ,and also the prevalence increase with age (40 – 60) 14 out of 15, most patients show gastritis and duodenal ulcer, 25 (60%) by endoscopy diagnosis and 7 (17%) show malignant cancer ,while 9(22%) without any symptoms. The sensitivity of urease test (82%) and specificity (88.1%) and by ICM sensitivity (86%) and specificity (67%) comparing with gold standard methods 100% . The aim of this study is to compare the different diagnostic techniques of Helicobacter pylori infection by using invasive methods (histological examination of gastric & duodenal biopsies stained by Giemsa &Haematoxilin & Eosin methods , & rapid urease test which is considered the gold standard methods & non-invasive serological methods such as ICM rapid test , all these tests provide information about the incidence and prevalence of H. pylori in population , diagnostic value for each test also the eradication of person.

يتضمن البحث دراسة انتشار بكتريا Helicobacter pylori المسببة لقرحة المعدة والاثنى عشري في عينة عشوائية من المرضى الذين ادخلوا إلى شعبة الناظور في مستشفى مدينة الطب التعليمي في بغداد وذلك باستخدام طرق تشخيصية مختلفة منها الفحص النسيجي باستخدام صبغة الهيماتوكسيلين وصبغة الكمزا وذلك لدراسة التغيرات النسيجية وفحص تواجد البكتريا في النسيج , كذلك فحص العينات بطريقة التحقق من إنتاج إنزيم اليورياز بالاعتماد على وسط اليوريا السائل الحاوي على دليل الفينول الأحمر وكذلك بالطريقة الغير مباشرة بالفحص السير ولوجي للأجسام المضادة IgG بالاعتماد على الطريقة السريعة ICM , وقد وجد ان نسبة الانتشار والإصابة تزداد مع تقدم العمر وخاصة بين الأعمار 45-60 سنة وان الإصابة بالرجال 44% نسبيا أعلى من الإناث 39% . ان التشخيص الأولي بالناظور يبين انه المرضى المصابين بقرحة المعدة والاثنى عشري هم حوالي 60% بينما المصابين بسرطان المعدة 22% والمرضى العاديين 17%وان اعلى نسبة للانتشار شخصت بطريقة الدراسة النسيجية 83% بينما كانت 73% بالطريقة السيرولوجية و 66% عن طريق فحص انزيم اليورياز , اما كفاءة وحساسية هذه الطرق فتتراوح بين 100% بالدراسة النسيجية و 86% , 82% بالطرق السيرولوجية والانزيمية على التواليز.


Article
Detection of CagA in H. Pylori Gastric Illness Using in Situ Hybridization

Authors: Nidhal A. Mohammed --- Ahmed S. Abood
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 85-90
Publisher: Diyala University جامعة ديالى

Loading...
Loading...
Abstract

Background: H. pylori had been recognized as the causative agent of several gastric diseases and possessed wide variety of virulence factors, one of most important factors is CagA. Objective: This study investigated the expression of CagA cytotoxin on the gastric epithelial cells in patients with Helicobacter pylori gastric illness by using In Situ Hybridization technique. Methods: Paraffin embedded tissue had been made form biopsies taken from 30 patients undergo O.G.D. and selected according to exclusion criteria. In addition 10 apparently healthy volunteers were included as a control group. Results: The CagA cytotoxin was detected in 21(70.0%) patients out of 30 and 9 (30.0%) gave negative results, on other hand control group showed no positive results. Conclusions: The CagA positive H. pylori strains cause the majority of H. pylori related gastric illness.


Article
Helicobacter Pylori Infection in Patients with Hematological Diseases

Author: Najlaa Badir Al-Awadi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 1 Pages: 74-79
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori (H. pylori) is a well known bacterial pathogen implicated in gastric diseases. Some studies refer to it's possible role in some hematological diseases.OBJECTIVE:The aim of this study is to determine the association between Helicobacter pylori infection and some hematological diseases.MATERIALS AND METHODS:This is a case-control study of 337 patients with a hematological disease (including acute and chronic leukaemias, Hodgkin's and non Hodgkin's lymphomas, hairy cell leukaemia, multiple myeloma, immune thrombocytopenic purpura and aplastic anemia) who were admitted to or attended hematology center at Marjan Teaching Hospital – Babylon – Iraq during the period from 1/7/2006 to 1/1/2009.Age and sex matched control group of 337 patients who have no hematological disease. They were taken during the same period and from the same geographical area (Babylon governorate).Tests for H. pylori were done for both groups using One Step H. pylori Test Device with 93.7% total accuracy rate.RESULTS:H. pylori test was positive in 33.5% (113/337) and 37.3% (126/337) of control and patient groups respectively, while dyspeptic symptoms were found in 18.1% (61/337) and 39.4% (133/337) of control and patient groups respectively.CONCLUSION:There was no significant association between H. pylori infection and hematological diseases tested in this study in Iraqi patients from Babylon, however dyspeptic symptoms were more common in patients on steroids or chemotherapy

Listing 1 - 6 of 6
Sort by
Narrow your search

Resource type

article (6)


Language

English (5)

Arabic (1)


Year
From To Submit

2010 (6)