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Article
Estimation of Immunoglobulin (IgG and IgM) in Hepatitis B Virus Infected Patients and Individuals Vaccinated with Recombinant HB Vaccine

Authors: Hiwa Abdul -Rahman Ahmed --- Rukia Muhammad Al-Barzinji
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 3 Pages: 257-261
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Hepatitis B virus (HBV) infection is a major public health problem. Successful clearance of the virus depends on a complex interaction between the virus and the host immune response.OBJECTIVE:The purpose of this study was to assessment of some immunoglobulins (IgG and IgM) in hepatitis patients and vaccinated groups compared with healthy control.METHODS:Study groups were classified into patient group 35 (15 acute (AC) and 20 chronic (CH)) and 35 vaccinated group (20 responder (RD) and 15 Non-responder (NRD)) and 18 healthy non vaccinated control group (HN). This study extended through a period from May to November 2007. Blood samples were taken from patients and hospitals staffs in Nanakaly, Erbil and Rizgary Teaching Hospital/ Erbil/ Iraq to detection of IgG and IgM level in serum by immunoturbidimetric test.RESULTS:The mean serum IgG level significantly increased in CH patients (p<0.05) compared to AH and HN control groups, in contrast IgM level increased high significant (p<0.001) in AH than CH and HN groups. No significantly differences in serum immunoglobulins (IgG and IgM) between vaccinated individuals and HN group (p>0.05).CONCLUSION:Present study indicate that serum IgG level was higher in CH patients whereas IgM level increased in AH. No significant differences in their levels were observed between vaccinated individuals and HN group.

Keywords

hepatitis --- immunoglobulin --- chronic --- acute --- vaccine


Article
EXTRACTION AND PURIFICATION OF IMMUNOGLOBULIN –G FROM HUMAN BLOOD SERUM
إستخلاص وتنقية الكلوبيولين المناعي صنفG من مصل دم الانسان

Author: عصام فاضل الجميلي1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 3 Pages: 578-591
Publisher: Baghdad University جامعة بغداد

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Abstract

Immunoglobulin G has been extracted from human blood serum by using ammonium sulphate salt in saturation percentage ranged between 0-35%, then immunoglobulin were separated by using ion exchange chromatography with the use of DEAE-Cellulose exchange and to complete separation and purification, gel filtration chromatography technique has been used with Sephadex G-200 column for purification of IgG. The molecular weights of the extracted immunoglobulins was identified with the use of Sephacryl S-200 column, and it was found that the molecular weight of IgG which was extracted from human blood serum was found to be 152 KD. The extracted immunoglobulins concentration was also determined with Bradford method were the concentration was about 9.5% mg/ml. The radial immunodiffusion test was done during all the stages of extraction, separation and purification to insurance degree of purity of immunoglobulins after every stage.

أستخلص الكلوبيولين المناعي صنف G من بلازما دم الانسان بطريقة الترسيب بكبريتات الأمونيوم بنسبة إشباع30 %. بعدها فصلت الكلوبيولينات المناعية بإعتماد تقنية كروماتوغرافيا التبادل الأيوني بإستعمال مبادل ثنائي أثيل امينو اثيل سليلوز DEAE-Cellulose. ولإتمام عملية التنقية أستعملت كروماتوغرافيا الترشيح الهلامي هلام Sephadex G-200 لتنقية الكلوبيولين المستخلص. أكدت النقاوة الكلوبيولين المناعي المستخلص بطريقة الترحيل الكهربائي بإستعمال هلام الاكريل امايد المتعدد عند ظروف غير ماسخة للبروتين. تم تعيين الوزن الجزيئي للكلوبيولين المناعي صنف G المستخلص من بلازما الدم بإستعمال عمود Sephacryl S-200 ووجد أن وزنه الجزيئي يبلغ 152 كيلو دالتن. قدر تركيز الكلوبيولين المستخلص بإستعمال الطريقة برادفورد فكان 9.5مليغرام/مليلتر. كما أجري فحص الانتشار المناعي الشعاعي خلال جميع مراحل الاستخلاص والفصل والتنقية للتأكد من درجة نقاوة البروتين بعد كل مرحلة.

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