research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
BLOOD PROGESTERONE AND ESTROGEN HORMONES LEVEL DURING PREGNANCY AND AFTER BIRTH IN IRAQI SHEEP AND GOAT
قياس مستوى هورموني البروجسترون والاستروجين خلال الحمل وبعد الولادة

Loading...
Loading...
Abstract

Serum progesterone and estrogen concentrations were investigated during pregnancy and few days after birth. Blood samples were collected twice / month from 24 numbered animals ( 12ewes and 12 does) . serum was isolated and kept under -20C untill hormonal analysis. Enzyme - Linked Immunosorbent assay (ELISA) using (ELISA Reader Dona 3200). Progesterone concentrations of pregnant ewes and does were showed steadily increased to reach 24.9+ 2.5 ng/ml and 30.34+ 2.3ng/ml in ewes and does, during 4th month and declined to 0.6 and 2.5 ng/ ml after birth in ewes and does, respectively. Estrogen hormone levels in ewes were increased significantly during pregnancy to maximum 98.7+4.3ng/ml by 5th month and sharply declined to 4.1+0.06 after birth. While in does estrogen level increased significantly and steadily to 1150.6+ 6.23 pg/ml during last month of pregnancy and to 5.9+ 0.4 pg/ml after birth. The present work indicated levels of progesterone hormone increased during 1st and 2nd months in ewes and does, while the significant increased from 3rd month on . Estrogen reach maximum concentrations during last month in doe Which higher about 11 times than that of ewes .It is useful means to diagnosis pregnancy of ewes and does by hormonal methods after mating 20-30 days.

يهدف البحث لقياس مستوى هرموني الحمل البروجسترون والاستروجين وبعد الولادة. جمع الدم من 24 رأس (12 نعجة و12 معزة )مرتين في ألشهر من كل حيوان . فصل وحفظ مصل الدم وتم قياس الهرمونات بطريقة Enzyme –linked immunosorbent assay(ELISA) وباستخدام (ELISA Reader Dona 3200) أعلى مستوى لهرمون البروجسترون كان في الشهر الرابع من الحمل 24.9+2.53 و 30.34+2.23 نانوعرام /مل في الأغنام والمعز وينخفض بصورة حادة بعد الولادة الى 0.6 و 2.5 نانوغرام /مل في الأغنام والماعز على التوالي . أما هرمون الاستروجبن في الأغنام والماعز فكان يزداد بصورة تصاعدية ومعنوية خلال أشهر الحمل ليصل إلى أعلى مستوى له في الأغنام 98.65+ 2.4 والماعز 1150.6+ 6.23 بيكوغرام / مل خلال الشهر الأخير من الحمل . بعد الولادة ينخفض إلى اقل مستوى فيصل 4.1 للأغنام و 5.9 للماعز. نستنتج من هذه الدراسة بان هرمون البروجسترون يرتفع خلال الشهر الثاني والثالث ليصل إلى أعلى مستوى للأغنام والماعز خلال الشهر الرابع للحمل .هرمون الاستروجين في الماعز يزداد خلال الحمل تصاعديا ويصل أعلى مستوى قبل الولادة و يصل مستو الهرمون 11 مرة أكثر مما في النعاج . من المفيد تشخيص الحمل في الأغنام والماعز بقياس هرمونات البروجستون والاستروجين بعد التسفيد أو التلقيح بـ 20-30 يوم. خلال أشهر الحمل

Keywords

: progesterone --- ELISA --- pregnancy


Article
Correlation of interleukin 6 (IL-6) with estrogen and progesterone receptor expression in breast cancer patients

Author: Amal K. Chaloob امل جلوب
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 4 Pages: 438-440
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Multifunctional cytokines play important and only partially defined roles in mammary tumor development and progression. Normal human mammary epithelial cells constitutively produce interleukin 6(IL-6) and a non-secreted form of tumor necrosis factor. Transformation of mammary epithelial cells by different oncogenesis is frequently associated with alterations of cytokine/ growth factor production and responsiveness. Methods: We measured levels of 1L-6 in 84 females with breast cancer and examined their correlation with clinicopathological variables including stages of the disease and estrogen and progesterone receptor expression on tumor cell.Results: Our results revealed significantly higher serum IL-6 in breast cancer patients (12.98 pg/ml) compared to a healthy group (2.05pg/ml) (P<0.05) and a direct association with different clinical stages. And we found that expression of this cytokine was inversely associated with estrogen and progesterone receptor cells.Conclusion: Serum levels of IL-6 are highly elevated in breast cancer patients and correlate with tumor progression. Assay for serum levels IL-6 can be used as predictive non-invasive tests for tumor progression in breast cancer patients; also our data suggest that cytokine could be involved in the aggressiveness of ER-PR negative breast tumors. Expression of interleukin 6(IL-6) correlates with estrogen and progesterone receptor in breast cancer patients. Keyword: Interleukin 6, breast cancer, estrogen-progesterone receptor

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2010 (2)